CN103211677A - Retina extractor - Google Patents
Retina extractor Download PDFInfo
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- CN103211677A CN103211677A CN2013101621847A CN201310162184A CN103211677A CN 103211677 A CN103211677 A CN 103211677A CN 2013101621847 A CN2013101621847 A CN 2013101621847A CN 201310162184 A CN201310162184 A CN 201310162184A CN 103211677 A CN103211677 A CN 103211677A
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- spoon
- extractor
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Abstract
The invention relates to a retina extractor used for an operation in the experimental ophthalmology. The retina extractor comprises a handle, a retina extracting spoon is arranged at one end of the handle, and a blade is arranged at the other end of the handle. A certain inclination angle is formed between the plane where the retina extracting spoon is located and an axis of the handle. A blade point is arranged at the front end of the blade. Insertion grooves are formed in the two ends of the handle respectively, and matched with the retina extracting spoon and the blade. Devices are arranged in the insertion grooves and used for fixing the retina extracting spoon and the blade. The handle, the extracting spoon and the blade are designed integrally, the design is exquisite and reasonable and meets the body mechanics principle, acquisition time of the retina can be greatly shortened, a using method and steps are simple and convenient to implement, and good conditions are created for the survival rate of nerve cells of the retina. The retina extractor is suitable for being used by researchers and medical workers at different levels in the field and worthy of being popularized.
Description
Technical field
The present invention relates to a kind of surgery medical apparatus and instruments, particularly relate to the retina extractor of operation usefulness in a kind of experimental eye science.
Background technology
Retina is the pith of eye structure, is the layer of transparent thin film that is positioned at the wall of eyeball internal layer, is made up of layer of retina,pigment epithelium and cerebral stratum of retina.The human information of obtaining from the external world comes from visual system more than 80%.Statistics shows that the whole world has 1.6 hundred million visual disorder patients approximately, 4,000 ten thousand legal blind persons.China is the maximum country of whole world blind person, has 7,000,000 approximately, and China has 450,000 people blind every year approximately, the injury that causes because of blinding oculopathy and can't estimate to the heavy burden that society causes.Retinal diseases is the common cause (surpassing 50%) of developed country's blinding, and blind about 200,000 (the accounting for 45%) of patient of the annual therefore class disease of China are the serious blinding oculopathy of still not having effective treatment means at present in the world.Therefore, carry out the research of retinal development and gene regulation rule energetically, for the pathogenesis of retinal diseases with to seek the research of therapy measure extremely urgent.
At present, in the visual science research field, the amphiblestroid common method of external acquisition is that these two kinds of methods are all consuming time longer, generally need 15-25 minute with enzymic digestion and microdissection separation method both at home and abroad.Facts have proved, after retina leaves live body 15-25 minute, the mortality rate of retinal neuronal cell can reach 40-60%, can this successfully will produce considerable influence to the retina basis of next carrying out with clinical research, very likely, cause the serious consequence of research failure because the retina isolated time is long.Therefore, be badly in need of improving existing retina and take out technology,, improve the survival rate of retinal neuronal cell, so that obtain real result of study, for basis and the clinical research of preventing blind blinding provides technical support so that can take out retina fast and efficiently.
Summary of the invention
The technical problem to be solved in the present invention: overcome the problem that the time is long, the retinal neuronal cell survival rate is low of drawing materials of retina in the prior art, a kind of retina extractor that can obtain retina fast, improve the retinal neuronal cell survival rate is provided.
Technical scheme of the present invention:
A kind of retina extractor comprises handle, and described handle one end is provided with retina and takes out spoon, and the other end is provided with blade, and described retina takes out spoon plane, place and handle axis is certain inclination angle, and the front end of described blade is provided with point of a knife.
The angle of described point of a knife is the 20-30 degree, and described inclination angle is the 15-30 degree.
Described retina takes out between spoon and the handle and is flexible connection between blade and the handle.
Described retina takes out spoon and takes organic plastics to make, and the spoon cross section that retina takes out spoon is circular, and diameter is 2.5-20mm.
Described handle adopts organic plastics or metal material to make, and the handle cross section is circular, and its diameter is 6-8mm, and handle is long to be 80-100mm.
Described retina takes out spoon and blade is disposable product.
Described handle two ends are respectively equipped with retina and take out the insertion groove that spoon and blade are complementary, and are provided with the device of fixedly retina taking-up spoonful and blade in the insertion groove.
The present invention has following advantage:
(1) retina extractor of the present invention, an end that adopts handle is that a retina taking-up spoon other end is the global design of blade, retina takes out spoon plane, place and handle place straight line is certain inclination angle, this inclination angle is different and difference to some extent according to the kind in retina source, the inclination angle that general meiofauna uses is the 15-20 degree, and the inclination angle that larger animal or people's eyeball use is about 30 degree.This deft design is reasonable, meets body mechanics's principle, can shorten the amphiblestroid acquisition time greatly, makes retina take-off time 15-25 minute by conventional method, shortens to about 5 seconds; Removing method and step are very easy, have avoided the phenomena of mortality of retinal neuronal cell in the retina taking-up process greatly, for the survival rate that improves retinal neuronal cell has been created good condition.
(2) a spoon cross-sectional diameter of retina taking-up spoon of the present invention is 2.5-20mm, both be fit to the less animal of eyeball and used (the about 4mm of eyeball of mouse diameter), also be fit to the bigger animal or human of eyeball and use (about 20 mm of Fructus Foeniculi Oculus sus domestica bulb diameter, the about 22mm of human eye bulb diameter), the scope of application is wider.
(3) retina of the present invention takes out between spoon and the handle, all adopts between blade and the handle and flexibly connect, and takes out spoon and blade and can adopt disposable product, can avoid repeated use, effectively prevents the mutual pollution between the species or between the cell.
(4) the present invention is easy to use, and method is easy to grasp, and is suitable for this area research worker at different levels and medical personnel and uses, and is worthy to be popularized very much.
(5) application of the present invention for amphiblestroid pathogenesis, retinal development and gene regulation Study on Mechanism, has been created favourable technical support.
Description of drawings
Fig. 1, retina extractor structural representation of the present invention;
The decomposition texture sketch map of Fig. 2, Fig. 1;
Fig. 3, perspective view of the present invention;
Among the figure: 1 for retina takes out spoon, and 2 is handle, and 3 is blade.
The specific embodiment
Embodiment 1The retina extractor referring to Fig. 1-3, comprises handle, and handle one end is provided with retina and takes out spoon, and the other end is provided with blade; The long 80mm of handle wherein, its cross section are circular, the about 6mm of diameter, and promptly handle is a cylindrical-shaped structure; The spoon cross section that retina takes out spoon is circle, and diameter is 2.5-10 mm, and the angle that takes out spoon plane, place and handle place axis is 15 degree; Blade is provided with sharp point of a knife, and the angle at point of a knife place is 30 degree.
The handle two ends are provided with insertion groove, and handle and retina take out and all adopt inserted mode to be connected between spoon, handle and the blade and fix.Handle adopts rustless steel or organic plastics to make.
The using method of retina extractor:
At first, with the phosphate buffer (pH7.4) of pre-cooling with contain antibiotic normal saline and fully wash the eyeball that derives from laboratory animal; Eyeball is placed on the eyeball fixer; Wipe out the affiliated group and the courageous and upright attachment of eyeball surface, keep four rectus, then four rectus are fixed on the eyeball fixer, cut complete all walls of eyeball along aequator oculi, remove anterior chamber of eye with the blade of retina extractor; Take out spoon with retina crystalline lens and vitreous body are taken out, reuse takes out spoon along the complete retina of retina cavity of resorption taking-up, and retina is put in advance in pre-cooling eppendorf pipe on ice, and whole experiment can be finished in 5 seconds.Obtain to carry out next step relevant experimentation behind the retina.
Embodiment 2Basic identical with embodiment 1, difference is:
The long 90mm of the handle of retina extractor, handle cross-sectional diameter are 7mm, and material adopts organic plastics; A retina spoon diameter is 11-16mm, and the angle that retina takes out spoon plane, place and handle axis is 20 degree,
Handle and retina take out and adopt existing spring gim peg or spring leaf to fix between spoon, handle and the blade.The angle at point of a knife place is 25 degree, and retina takes out spoon and blade all adopts disposable material to make.
Embodiment 3Basic identical with embodiment 1, difference is:
The long 100mm of the handle of retina extractor, handle cross-sectional diameter are 8mm; A spoon diameter 17-20mm, the angle that takes out spoon plane, place and handle axis are 30 degree, and point of a knife place angle is 20 degree.Retina takes out spoon employing organic plastics and makes.
Claims (7)
1. a retina extractor comprises handle, it is characterized in that: described handle one end is provided with retina and takes out spoon, and the other end is provided with blade, and described retina takes out spoon plane, place and handle place axis is certain inclination angle, and the front end of described blade is provided with point of a knife.
2. retina extractor according to claim 1 is characterized in that: the angle of described point of a knife is the 20-30 degree, and described inclination angle is the 15-30 degree.
3. retina extractor according to claim 1 is characterized in that: described retina takes out between spoon and the handle, be flexible connection between blade and the handle.
4. retina extractor according to claim 1 is characterized in that: described retina takes out spoon and takes organic plastics to make, and its spoon cross section is circular, and diameter is 2.5-20mm.
5. retina extractor according to claim 1 is characterized in that: described handle adopts organic plastics or metal material to make, and the handle cross section is diameter 6-8mm, and handle is long to be 80-100mm.
6. retina extractor according to claim 1 is characterized in that: described retina takes out spoon and blade is disposable product.
7. according to each described retina extractor of claim 1-6, it is characterized in that: described handle two ends are respectively equipped with retina and take out the insertion groove that spoon and blade are complementary, and are provided with the parts of fixedly retina taking-up spoonful or blade in the insertion groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310162184.7A CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310162184.7A CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
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| Publication Number | Publication Date |
|---|---|
| CN103211677A true CN103211677A (en) | 2013-07-24 |
| CN103211677B CN103211677B (en) | 2015-03-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310162184.7A Active CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014180272A1 (en) * | 2013-05-06 | 2014-11-13 | 郑州大学第一附属医院 | Method for isolating and preparing retinal nerve cell layer in vitro |
| CN111925988A (en) * | 2020-08-29 | 2020-11-13 | 郑州大学 | Method for preparing single cell suspension based on retina tissue/organoid-retina tissue |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2071060U (en) * | 1990-05-31 | 1991-02-13 | 孙承禄 | Eye muscle choroid insertion operation knife |
| CN103006374A (en) * | 2012-12-27 | 2013-04-03 | 尹澜 | Corneal foreign body draw-out spoon |
| CN203195839U (en) * | 2013-05-06 | 2013-09-18 | 郑州大学第一附属医院 | Retina taking-out instrument |
-
2013
- 2013-05-06 CN CN201310162184.7A patent/CN103211677B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2071060U (en) * | 1990-05-31 | 1991-02-13 | 孙承禄 | Eye muscle choroid insertion operation knife |
| CN103006374A (en) * | 2012-12-27 | 2013-04-03 | 尹澜 | Corneal foreign body draw-out spoon |
| CN203195839U (en) * | 2013-05-06 | 2013-09-18 | 郑州大学第一附属医院 | Retina taking-out instrument |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014180272A1 (en) * | 2013-05-06 | 2014-11-13 | 郑州大学第一附属医院 | Method for isolating and preparing retinal nerve cell layer in vitro |
| CN111925988A (en) * | 2020-08-29 | 2020-11-13 | 郑州大学 | Method for preparing single cell suspension based on retina tissue/organoid-retina tissue |
| CN111925988B (en) * | 2020-08-29 | 2022-09-23 | 郑州大学 | Method for preparing single cell suspension based on retina tissue/organoid-retina tissue |
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| Publication number | Publication date |
|---|---|
| CN103211677B (en) | 2015-03-18 |
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