CN103211145B - Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide - Google Patents
Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide Download PDFInfo
- Publication number
- CN103211145B CN103211145B CN201310107984.9A CN201310107984A CN103211145B CN 103211145 B CN103211145 B CN 103211145B CN 201310107984 A CN201310107984 A CN 201310107984A CN 103211145 B CN103211145 B CN 103211145B
- Authority
- CN
- China
- Prior art keywords
- peptide
- polysaccharide peptide
- active polysaccharide
- polysaccharide
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of active polysaccharide peptide with protective effect to alcoholic liver injury and a compound of the active polysaccharide peptide. The method is characterized by comprising the following steps of: preparing polysaccharide peptide by processes of smashing, alcohol precipitation, degreasing, centrifugation, composite enzymatic hydrolysis, centrifugal separation and the like on fresh balsam pear and maize yellow powder, wherein the proportion of polysaccharide to maize peptide is 1:3; and then regulating flavor by malic acid and xylitol to obtain a polysaccharide peptide alcohol dispelling and liver protecting oral liquid. on the basis that alanine and leucine concentration in blood is improved to generate stable NAD+ so as to effectively reduce ethanol concentration in blood, the inhibition ratio of hydroxyl radicals reaches 100%, and liver cell mitochondria is prevented from being oxidized and injured. The process of the active polysaccharide peptide is simple and easy to operate; and the active polysaccharide peptide has high extraction content, high absorption rate, strong function and the like; therefore, great social and economic benefits are achieved.
Description
Technical field
The present invention relates to and relate to a kind of biological products, particularly a kind of preparation method alcoholic liver injury to the active polysaccharide peptide of protective action and composite.
Background technology
The AML (Alcoholic liver Disease, ALD) that alcoholic liver injury causes is occurred frequently in states such as America and Europes, and along with the change of China's economic development and people life style, China ALD incidence of disease is in ascendant trend year by year.In the cooking culture of China, have dinner to drink, drink then often liquor-saturated, excessive consumption of alcohol causes hepatic injury.Alcoholic liver cause of disease is the common disease of developed country, but also reaches the degree that can not be ignored in recent years in China.According to statistics, in China wine people, the alcoholic liver injury incidence of disease is about 20%, and AML is own through becoming the second bugle look be only second to after the virus hepatitis such as hepatitis A, hepatitis B hepatopathy.The exploitation of current western countries antialcoholic drugs is still based on synthetic drug, and it is long with the drug therapy alcoholic liver injury cycle, and synthesis class medicine itself has toxic and side effect, in liver, metabolism again can liver injury further, so the antialcoholic drugs developed by synthetic method for many years is not made substantial progress.And the states such as China, Japan and Korea excavate the active material in natural products energetically on the basis of inheriting tradition medicine, some impressive progresses are achieved in some aspects, for the functional food developing facilitating alcohol metabolism and protecting liver opens wide prospect.
Acute alcoholism can cause hepatic injury, one of its damage mechanisms produces the peroxide such as a large amount of ultra-oxygen anion free radical, hydroxy radical by excited oxygen molecule, and exceed intracellular superoxide mutase (SOD), peroxidase (CAT), glutathione peroxidase (GSH2px), the detoxification ability of the body self-defence detoxification systems such as glutathione (GSH) and vitamin E, must cause serious harm to organelles such as mitochondria, endoplasmic reticulum and lysosomes, cause degeneration of liver cells, necrosis.For the pathogenesis of acute alcohol hepatic injury, the research and development of antialcoholic drugs are point of penetration mainly with anti-oxidation characteristics.Deeper research shows, superoxide anion is the initiator of radical reaction, under transition elements exists, is converted into hydroxy radical rapidly.Hydroxy radical can participate in the chain reaction of mitochondrial membrane lipid layer polyunsaturated fatty acid generation lipid peroxidation directly, and destroy the structure of film fat, its end-product is small molecule aldehyde, hydroperoxides and alkane gas etc.Above-mentioned product can bring out mitochondria swelling again, changes mobility and the atpase activity of film, and these changes are all destroy the complete factor of hepatic mitochondria structure and fuction.Therefore, hydroxy radical is harm hepatic mitochondria 26S Proteasome Structure and Function central factor.
The representational antialcoholic drugs of traditional Chinese medicine most mainly contains FI puerariae, the root of kudzu vine, ginseng, wilsonii etc., mostly is China's rare traditional Chinese medicine.These antialcoholic drugs adopt extraction, infusion process, percolation, circumfluence method, distilled method etc. more on reparation technology, and its composition is mixture, and active ingredient is indefinite, and the antialcoholic drugs more not screening hepatoprotecting factor using hydroxy radical as target spot comes out.
Along with the fast development of food biotechnology, from the food materials or crops processed side product of integration of drinking and medicinal herbs, obtained the material of physiologically active of sobering up by biological enzymolysis technology, in order to promote that the research of liver alcohol metabolism becomes focus gradually.Corn Toplink activates Alcohol-metabolizing enzymes, and be the more biologically active peptide of sobering up reported in recent years, Japanese scholars Yamaguchi points out, corn peptide produces stable NAD by improving alanine and leucic concentration in blood
+and effectively reduce the concentration of ethanol in blood, and corn peptide has stronger resistance to oxidation, particularly it can reach 50% to the inhibiting rate of hydroxy radical, if cooperative effect agent can be added in corn peptide, in stimulation tissue, alcohol metabolism enzymatic activity promotes its antioxidation while increasing, then can increase substantially the effect of its prevention acute alcohol hepatic injury.
Polysaccharide is the natural macromolecular substances of a class, is almost present in all organisms, comprises animal, plant (mainly higher plant), microorganism (bacterium and fungi) and marine alga.Active polysaccharide refers to and is present in organism, can promote or strengthen body health, there is control Cell Differentiation, regulating the non-specific broad immune conditioning agent of a class of Growth of Cells aging, being the important living matter material of a class, is modern medicine and the common focus paid close attention to of food function chemistry.Research shows, polysaccharide can scavenging free radicals, and then prevents hepatic lipid peroxidation, has protective effect to liver.Active component in usual functional food is all monofactorial, and our research shows, is undertaken composite by corn antioxidant peptide and polysaccharide, its to hydroxy radical inhibiting rate can reach 100%.The two extraction process is organically combined, finds out the new technology of a set of polysaccharide-peptide compound beverage and derivative new product thereof, then can promote that alcohol metabolism produces a kind of compensatory effect to corn peptide, at home and abroad there is no and study report in this respect.
summary of the invention:
The object of this invention is to provide and a kind ofly to alcoholic liver injury, there is the active polysaccharide peptide preparation method of protective action and composite,
Namely with balsam pear, maize yellow-powder for raw material, make through enzymolysis, alcohol precipitation, centrifugation, the processing step such as composite, technique is simple, is easy to operation, extracts content high, and be easy to absorb, by force functional, cost is low, without any chemical addition agent.
The invention provides a kind of preparation method alcoholic liver injury to the active polysaccharide peptide of protective action, it is characterized in that step is as follows:
A. polysaccharide preparation: fresh bitter is cleaned and removes flesh, cut into slices and put into 50 DEG C of oven for drying, the balsam pear slice of oven dry is ground into dry powder, get bitter melon powder, cell-wall degrading enzyme solution is added according to feed liquid weight ratio 1:40, cell-wall degrading enzyme solution including fiber element enzyme 0.5%, pectase 0.8%, surplus is the citrate buffer solution of 0.05mol/L, pH4.5; In 50 DEG C of water-bath lixiviate 3h, extract gets supernatant after the centrifugal 10min of 4500r/min, precipitates after supernatant reduced pressure concentration with absolute ethyl alcohol, and by centrifugal for mixed liquor 7000r/min 10min, collecting precipitation redissolves, and adds active carbon clarification;
B. zeins modification: maize yellow-powder particle pulverizer is pulverized, 100 orders sieve, obtain zein powder, the sodium bicarbonate solution that mass concentration is 40g/L is added by feed liquid weight ratio 1:12, on shaking table, constant temperature 40 DEG C carries out ungrease treatment 20 minutes, by maize yellow-powder protein mixed solution in the centrifugal 10min of 7000r/min, get supernatant, zeins after reduced pressure concentration utilizes 0.01mol/L, the borate buffer of pH9.0 carries out first time enzymolysis, hydrolysising condition is: temperature is 37 DEG C, ethanol contend specific concentration is 70%, trypsase mass concentration is 8%, hydrolysis time is 3h,
C. peptidoglycan preparation: the ethanol heating in protein hydrolyzate is steamed completely, part by weight by 3: 1 gets the polysaccharide solution mixing after protein hydrolyzate and clarification, neutral proteinase is utilized to carry out second time enzymolysis, hydrolysising condition is: 55 DEG C, pH is 7.5, and the weight ratio of enzyme concentration and albumen is 100:4; Be hydrolyzed 50min with this understanding, namely obtain the peptidoglycan complex liquid with antioxidation activity.
The invention provides the composite of a kind of peptidoglycan oral liquid, it is characterized in that: in peptidoglycan complex liquid, add malic acid, xylitol or honey, three's part by weight is 100:0.1:6, can peptide complex function drink.
Tool of the present invention has the following advantages:
1, the function factor in functional food is usually more single, the present invention take natural plant resource as raw material, utilize enzyme engineering technology to prepare and there is synergistic polysaccharide and the difunctional factor of peptide, and carry out complex process optimization, promoting its anti-oxidant and promotion alcohol metabolism effect, is a kind of functional food of energy facilitating alcohol metabolism and protecting liver.
2, facilitating alcohol metabolism and protecting liver active component mostly derives from rare traditional Chinese medicine as ginseng, the root of kudzu vine etc., the present invention prepares polysaccharide-peptide complexes from the vegetable material of integration of drinking and medicinal herbs, cost is low, without any chemical addition agent, functional characteristic is strong, more there is the market competitiveness, balsam pear and corn resources deep processing and utilization can be driven.
Detailed description of the invention:
The preparation of active polysaccharide peptide:
1, remove flesh by clean for fresh bitter, cut into slices and put into 50 DEG C of oven for drying; The balsam pear slice of oven dry is ground into dry powder (crossing 100 mesh sieves).Take bitter melon powder 100 grams, the ratio being 1:40 according to solid-liquid ratio adds cell-wall degrading enzyme solution (including fiber element enzyme 0.5%, pectase 0.8%, surplus is 0.05mol/L, the citrate buffer solution of pH4.5) 4L is in 50 DEG C of water-bath lixiviate 3h, extract obtains supernatant 3.5L after the centrifugal 10min of 4500r/min, supernatant is evaporated to 350ml, in the centrifugal 10min of 7000r/min after precipitating with 3 times of volume absolute ethyl alcohols, collecting precipitation redissolves to obtain polysaccharide solution 100ml, add activated carbon 15g wherein, again with the centrifugal 3min of 10000r/min after decolouring 10min, polysaccharide solution can be made to clarify.
2, take 100g zein powder, add the sodium bicarbonate solution that 1.2L mass concentration is 40g/L, on shaking table, constant temperature 40 DEG C carries out ungrease treatment 20 minutes, by maize yellow-powder protein mixed solution in the centrifugal 10min of 7000r/min, get supernatant, reduced pressure concentration, weigh.Zein powder is obtained after degreasing, get that zeins 50 grams adds 1000ml 0.1mol/L, the borate buffer of pH 9.0 carries out first time alcohol phase enzymolysis, hydrolysis temperature is 37 DEG C, and ethanol contend specific concentration is 70%, trypsase mass concentration is 8%, and hydrolysis time is 3h.With this understanding, degree of hydrolysis can reach 18.84%, and proteolytic rate also can reach 80%.Through first step hydrolysis, modified zeins can be changed into water-soluble.
3, protein hydrolyzate is heated to 70 DEG C steam completely to ethanol, polysaccharide solution 30 ml got after protein hydrolyzate 90 ml and clarification mixes, and utilize neutral proteinase to carry out second time enzymolysis, hydrolysising condition is: 55 DEG C, pH is under the condition of 7.5, and the weight ratio of enzyme concentration and albumen is 100:4; Be hydrolyzed 50min with this understanding, now proteolytic rate can reach 95.55%.By hydrolyzate in the centrifugal 10min of 4000rpm, namely obtain the peptidoglycan complex liquid with antioxidation activity.
Peptidoglycan oral liquid composite:
In 100ml peptidoglycan complex liquid, add malic acid 0.1 gram, xylitol/honey 6 grams, Antialcoholic liver-protecting peptidoglycan complex function drink can be obtained.
Process implementing result
The polyoses oral liquid that the present invention obtains, proteolytic rate can reach 95.55%, and the concentration ratio of polysaccharide and anti-oxidation peptide is 1: 3, can reach 100% to the inhibiting rate of hydroxy radical, can promote alcohol metabolism and control liver cell mitochondria oxidative damage.
Claims (1)
1. alcoholic liver injury is had to a preparation method for the active polysaccharide peptide of protective action, it is characterized in that step is as follows:
A. polysaccharide preparation: fresh bitter is cleaned and removes flesh, cut into slices and put into 50 DEG C of oven for drying, the balsam pear slice of oven dry is ground into dry powder, get bitter melon powder, cell-wall degrading enzyme solution is added according to feed liquid weight ratio 1:40, cell-wall degrading enzyme solution including fiber element enzyme 0.5%, pectase 0.8%, surplus is the citrate buffer solution of 0.05mol/L, pH4.5; In 50 DEG C of water-bath lixiviate 3h, extract gets supernatant after the centrifugal 10min of 4500r/min, and precipitate after supernatant reduced pressure concentration with absolute ethyl alcohol, then carry out the centrifugal 10min of 7000 r/min, collecting precipitation redissolves, and adds active carbon clarification;
B. zeins modification: maize yellow-powder particle pulverizer is pulverized, 100 orders sieve, obtain maize yellow-powder albumen, the sodium bicarbonate solution that mass concentration is 40g/L is added by feed liquid weight ratio 1:12, on shaking table, constant temperature 40 DEG C carries out ungrease treatment 20 minutes, by maize yellow-powder protein mixed solution in the centrifugal 10min of 7000 r/min, get supernatant, reduced pressure concentration, zeins is obtained after degreasing, zeins utilizes 0.01mol/L, the borate buffer of pH9.0 carries out first time enzymolysis, hydrolysising condition is: temperature is 37 DEG C, ethanol contend specific concentration is 70%, trypsase mass concentration is 8%, hydrolysis time is 3h,
C. peptidoglycan preparation: the ethanol heating in protein hydrolyzate is steamed completely, the polysaccharide solution mixing after protein hydrolyzate and clarification is got by the part by weight of 3:1, neutral proteinase is utilized to carry out second time enzymolysis, hydrolysising condition is: 55 DEG C, PH is 7.5, and the weight ratio of enzyme concentration and albumen is 100:4; Be hydrolyzed 50min with this understanding, namely obtain the peptidoglycan complex liquid with antioxidation activity.
2. a kind of method utilizing the active polysaccharide peptide having a protective action to alcoholic liver injury described in claim 1 to carry out compound oral liquid, it is characterized in that: in 100ml peptidoglycan complex liquid, add malic acid 0.1 gram, xylitol/honey 6 grams, peptidoglycan complex function drink can be obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310107984.9A CN103211145B (en) | 2013-04-01 | 2013-04-01 | Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310107984.9A CN103211145B (en) | 2013-04-01 | 2013-04-01 | Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103211145A CN103211145A (en) | 2013-07-24 |
| CN103211145B true CN103211145B (en) | 2015-01-21 |
Family
ID=48809590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310107984.9A Expired - Fee Related CN103211145B (en) | 2013-04-01 | 2013-04-01 | Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103211145B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103989223B (en) * | 2014-04-24 | 2015-11-18 | 绍兴文理学院元培学院 | The preparation method of water chestnut beverage for dispelling alcoholic intoxication |
| CN110981952A (en) * | 2019-12-27 | 2020-04-10 | 齐齐哈尔大学 | Preparation method and application of lactoferrin modified peptide with protection effect on ethanol-induced damaged liver cells |
| CN113480608B (en) * | 2021-08-16 | 2022-09-02 | 合肥工业大学 | Dry-pickled ham-derived polypeptide capable of relieving alcoholic liver injury and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147537A (en) * | 2007-10-26 | 2008-03-26 | 华南理工大学 | Method for high efficiency extracting momordica polysaccharide from balsam pear |
| CN101411494A (en) * | 2008-11-28 | 2009-04-22 | 华南理工大学 | Corn peptide beverage for sobering-up and preparation method thereof |
| CN101461514A (en) * | 2009-01-22 | 2009-06-24 | 韩金光 | Bitter melon extract and preparation method thereof |
| CN102021216A (en) * | 2010-11-11 | 2011-04-20 | 湖北远成药业有限公司 | Extraction method of antialcoholism peptide |
| CN102960808A (en) * | 2012-12-05 | 2013-03-13 | 上海普维健康食品有限公司 | Alcohol-alleviating anti-fatigue beverage |
-
2013
- 2013-04-01 CN CN201310107984.9A patent/CN103211145B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147537A (en) * | 2007-10-26 | 2008-03-26 | 华南理工大学 | Method for high efficiency extracting momordica polysaccharide from balsam pear |
| CN101411494A (en) * | 2008-11-28 | 2009-04-22 | 华南理工大学 | Corn peptide beverage for sobering-up and preparation method thereof |
| CN101461514A (en) * | 2009-01-22 | 2009-06-24 | 韩金光 | Bitter melon extract and preparation method thereof |
| CN102021216A (en) * | 2010-11-11 | 2011-04-20 | 湖北远成药业有限公司 | Extraction method of antialcoholism peptide |
| CN102960808A (en) * | 2012-12-05 | 2013-03-13 | 上海普维健康食品有限公司 | Alcohol-alleviating anti-fatigue beverage |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103211145A (en) | 2013-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5269160B2 (en) | Bioactive liquid composition and method for producing the same | |
| Verma et al. | Biotechnological production of bioactive compounds | |
| CN101768621B (en) | Method for preparing sea-buckthorn enzymes from seabuckthorn fruit mud | |
| CN108887673B (en) | Preparation method of stauntonvine enzyme rich in superoxide dismutase (SOD) | |
| JP2002262820A (en) | Method for extracting active ingredient of mushrooms | |
| CN102246961B (en) | Ganoderma gingko functional food, preparation method and application | |
| Kowalczewski et al. | Bioactive compounds of potato (Solanum tuberosum L.) juice: From industry waste to food and medical applications | |
| CN103564197A (en) | Compound plant extract feed additive and preparation method thereof | |
| KR101468029B1 (en) | The preparing method of agents for improving liver function | |
| JP6534443B2 (en) | Method for producing bamboo fermented extract and method for producing immunostimulatory food composition or immunostimulant | |
| CN107125675A (en) | A kind of preparation method of blueberry residue flavouring-soy-sauce | |
| CN103211145B (en) | Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide | |
| Liu et al. | Characterization of selenium-enriched mycelia of Catathelasma ventricosum and their antihyperglycemic and antioxidant properties | |
| CN106418111A (en) | Antioxidant extract derived from garlic stalks and preparation method thereof | |
| US20110293760A1 (en) | Method for extracting substances from soapberry fruit and seed and products made therefrom | |
| CN108624636A (en) | A kind of preparation method of lentinan | |
| Atila | Cultivation and utilization of shiitake mushroom | |
| Sharma et al. | Utilization of waste from tropical fruits | |
| CN101830747B (en) | Culture medium for use in artificial culture of Tibetan Yatung wild black fungi | |
| JP5684989B2 (en) | Enzyme-treated and gonococcal fermentation-treated product | |
| CN105175565A (en) | Method for simultaneously producing black peanut proanthocyanidin and non-starch polysaccharide microcapsule | |
| KR20130010775A (en) | A functional health food having antioxidative activity and method for preparation thereof | |
| CN111165694B (en) | Composite black jerusalem artichoke functional nutrient solution and preparation method thereof | |
| CN103445151A (en) | Cordyceps militaris oral liquid and preparation method thereof | |
| Widyani | Potency of herbal plants formulation as anticholesterole agent: In vitro studies |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150121 Termination date: 20170401 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |