CN103205513A - Extraction method for fructose from waste dextran fermentation broth - Google Patents
Extraction method for fructose from waste dextran fermentation broth Download PDFInfo
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- CN103205513A CN103205513A CN2012103279116A CN201210327911A CN103205513A CN 103205513 A CN103205513 A CN 103205513A CN 2012103279116 A CN2012103279116 A CN 2012103279116A CN 201210327911 A CN201210327911 A CN 201210327911A CN 103205513 A CN103205513 A CN 103205513A
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- dextran
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Abstract
The invention discloses an extraction method for fructose from waste dextran fermentation broth. The method comprises the following steps: subjecting waste dextran fermentation broth to ultrafiltration or nanofiltration separation, removing charged ions and carrying out condensation so as to obtain high purity fructose syrup (containing 90 to 95% of fructose); or adding a modifier and carrying out programmed temperature controlling, separation and drying so as to obtain a fructose crystal substance. The extraction method is a process route with the advantages of a few steps and no generation of other by-products and enables high purity fructose syrup or crystalline fructose to be extracted from waste dextran fermentation broth.
Description
Technical field
The invention belongs to medical material technical field and chemical feedstocks technical field, be specifically related to a kind ofly from dextran-fermentation liquid waste liquid, extract fructose, prepare the method for high purity fructose syrup or crystal diabetin.
Background technology
Preparing in the dextran process with sucrose, finishing the fructose that contains in the dextran extraction dextran-fermentation liquid waste liquid afterwards and can supply extraction and application.But because fructose molecular structure instability is heated and just is decomposed into the furfural material of band look easily; Simultaneously, the impurity component in the dextran-fermentation liquid waste liquid is of a great variety, and the technical difficulty that makes fructose extract is quite big, does not still have a kind of other by products that do not produce in the prior art, makes the satisfied method of people.Set up a cover and extract fructose from dextran-fermentation liquid waste liquid, preparation purity is the fructose syrup of 90-95% or the industrialized producing technology of crystal diabetin, and not produce other by products are pressing for of present relevant industries.
Summary of the invention
The invention provides a kind of method of extracting fructose from dextran-fermentation liquid waste liquid, this method adapts to the characteristic of fructose molecule, can dna purity be high purity fructose syrup or the crystal diabetin of 90-95% from the numerous and diverse dextran-fermentation liquid waste liquid of composition.
The present invention extracts the method for fructose from dextran-fermentation liquid waste liquid, its processing step is in regular turn:
Dextran-fermentation liquid waste liquid is done ultrafiltration to steps A or nanofiltration is handled;
The solution of step B is made the nanofiltration concentration;
Step C handles through the yin, yang resin bed;
Step D cryoconcentration gets the high purity fructose syrup;
Step e adds the ethanol modification;
The smart filter of step F decolouring;
The crystallization of step G temperature programmed control, the fractional crystallization thing, cryodrying gets crystal diabetin.
In the above-mentioned processing method,
Steps A is done ultrafiltration or nanofiltration to dextran-fermentation liquid waste liquid and is handled used filter membrane and can select for use in ultra-filtration membrane, the nanofiltration membrane anyly, and the membrane pore size scope of application is 300-2000 dalton.
It is 50-200 dalton that step B does its aperture of the used film of nanofiltration concentration.
Step C handles solution through the yin, yang resin bed, to remove the charging property material.
The method of step D cryoconcentration can be selected falling liquid film cryoconcentration method or vacuum and low temperature method of enrichment, is 30%-80% with solution concentration to sugar-containing concentration, gets the high purity fructose syrup.This high purity fructose syrup can be made for foodstuff additive usefulness as finished product, also can continue following processing step as intermediates by the inventive method, makes crystal diabetin.
It is solvent modified to the high purity fructose syrup that step e adds ethanol, makes the high purity fructose syrup contain alcohol concn and reach 50%-70%.The employed ethanol of this processing step should be dehydrated alcohol or high concentration ethanol.
The method that the high purity fructose syrup of step F after to modification implemented decolouring is resin absorption decoloring method or charcoal absorption decoloring method; Used filter method can be selected for use in filter stick filtration method, the molten filtration method of hammer, centrifugal filtration process, vacuum suction filter method and the filter press method any.
The suitable temperature of step G temperature programmed control crystallization is 20 ℃--60 ℃, crystallization time is 50-120 hours, and crystal solution sugar concentration is 30%--85%.Use centrifuging or filtration method fractional crystallization thing, again crystallisate is implemented cryodrying and get the finished product crystal diabetin.The method of used cryodrying is any in microwave drying, vacuum-drying, ultra red ray drying, the fluidized drying method.
The enforcement order of above-mentioned steps A and step B can be exchanged, that is, after elder generation makes the nanofiltration concentration to dextran-fermentation liquid waste liquid, remake ultrafiltration or nanofiltration and handle; The enforcement order of above-mentioned steps E and step F can be exchanged, that is, the smart filter of decolouring in advance adds the ethanol modification again after making the high purity fructose syrup.Do not influence the fructose quality product after the processing step transposing.
The alleged dextran-fermentation liquid of the inventive method refers to prepare by sucrose fermentation traditional technology method the fermented liquid of dextran gained.The inventive method is applicable to that also the molecular Control legal system gets the dextran waste liquor and the prepared by enzyme engineering that obtain behind the dextran and get the waste liquid that obtains behind the dextran, therefrom extracts high purity fructose syrup or crystal diabetin.
Compared with prior art, the present invention extracts fructose from dextran-fermentation liquid waste liquid method is easy relatively, the equipment less investment, yield is higher, do not produce other by products, be applicable to general medium-sized and small enterprises, avoided dextran-fermentation liquid waste liquid to cause environmental pollution as waste discharge simultaneously.
Embodiment
By the following examples technical scheme of the present invention is described in further detail.
Embodiment 1Get pol and be 4% dextran-fermentation liquid waste liquid 10000ml, through 800 dalton's nanofiltration membrane treatment, solution is crossed the yin and yang resin bed, being evaporated to pol below 75 ℃ is 80%, adds dehydrated alcohol 40ml, adds activated carbon decolorizing, smart filter, temperature programmed control, obtain the fructose crystallization when solution is cooled to 25 ℃ from 55 ℃, filter the wet product of fructose, about vacuum-drying gets fructose crystallisate dry product 85 and restrains.
Embodiment 2Get pol and be 3.5% dextran-fermentation liquid waste liquid 20000ml, through 500 daltonian nanofiltration membrane treatment, through concentration, getting pol is 10.6% solution, liquor capacity is reduced to about 6500ml, and solution is crossed the yin and yang resin bed, and being evaporated to pol below 75 ℃ is 83%, add dehydrated alcohol 85ml, add activated carbon decolorizing, smart filter, when temperature programmed control, solution are cooled to 25 ℃ from 55 ℃, filter the wet product of fructose, about vacuum-drying gets fructose crystallisate dry product 155 and restrains.
Embodiment 3Get pol and be 4.5% dextran-fermentation liquid waste liquid 20000ml, through 300 daltonian nanofiltration membrane treatment, again through concentration, getting pol is 12.5% solution, liquor capacity is reduced to about 7000ml, and solution is crossed the yin and yang resin bed, and being evaporated to pol below 78 ℃ is 81%, add dehydrated alcohol 85ml, add activated carbon decolorizing, smart filter, when temperature programmed control, solution are cooled to 25 ℃ from 55 ℃, filter the wet product of fructose, can get fructose crystallisate dry product 201 through vacuum-drying and restrain.
Embodiment 4Get pol and be 3.5% dextran-fermentation liquid waste liquid 20000ml, through 1000 daltonian ultrafiltration membrane treatment, again through concentration, getting pol is 9.4% solution, solution is crossed the yin and yang resin bed, add activated carbon decolorizing, smart filter, be evaporated to pol below 75 ℃ and be about 82% high purity fructose syrup 810ml.
Embodiment 5Get pol and be 4% dextran-fermentation liquid waste liquid 10000ml, through 1500 daltonian ultrafiltration membrane treatment, again through concentration, solution is crossed the yin and yang resin bed, add activated carbon decolorizing, smart filter, be evaporated to pol below 75 ℃ and be about 80% high purity fructose syrup 460ml.
Claims (10)
1. method of from dextran-fermentation liquid waste liquid, extracting fructose, its processing step is in regular turn:
Dextran-fermentation liquid waste liquid is done ultrafiltration to steps A or nanofiltration is handled;
The solution of step B is made the nanofiltration concentration;
Step C handles through the yin, yang resin bed;
Step D cryoconcentration gets the high purity fructose syrup;
Step e adds the ethanol modification;
The smart filter of step F decolouring;
The crystallization of step G temperature programmed control, the fractional crystallization thing, cryodrying gets crystal diabetin.
2. the method for from dextran-fermentation liquid waste liquid, extracting fructose according to claim 1, it is characterized in that, steps A is done ultrafiltration or nanofiltration to dextran-fermentation liquid waste liquid, and to handle used filter membrane be any in ultra-filtration membrane, the nanofiltration membrane, and membrane pore size is 300-2000 dalton.
3. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that it is 50-200 dalton that step B does its aperture of the used film of nanofiltration concentration.
4. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that, the method for step D cryoconcentration is falling liquid film cryoconcentration method or decompression cryoconcentration method, and solution concentration to sugar-containing concentration is 30%-80%.
5. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that, step e is after adding dehydrated alcohol or high concentration ethanol, and it is 50%-70% that the high purity fructose syrup contains alcohol concn.
6. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that, the method that the high purity fructose syrup of step F after to modification implemented decolouring is resin absorption decoloring method or charcoal absorption decoloring method.
7. the method for from dextran-fermentation liquid waste liquid, extracting fructose according to claim 1, it is characterized in that the method that the high purity fructose syrup of step F after to modification implemented smart filter is any in the molten filtration method of filter stick filtration method, hammer, centrifugal filtration process, vacuum suction filter method or the filter press method.
8. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that the temperature of step G temperature programmed control crystallization is 20 ℃--60 ℃, crystallization time is 50-120 hours, and crystal solution sugar concentration is 30%--85%.
9. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that the method for step G fractional crystallization thing is centrifuging or filtration method.
10. the method for extracting fructose from dextran-fermentation liquid waste liquid according to claim 1 is characterized in that the enforcement of steps A and step B is exchanged in proper order, that is, after elder generation makes the nanofiltration concentration to dextran-fermentation liquid waste liquid, remake ultrafiltration or nanofiltration and handle; The enforcement of step e and step F is exchanged in proper order, that is, the smart filter of decolouring in advance adds the ethanol modification again after making the high purity fructose syrup.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103279116A CN103205513A (en) | 2012-09-07 | 2012-09-07 | Extraction method for fructose from waste dextran fermentation broth |
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| CN2012103279116A CN103205513A (en) | 2012-09-07 | 2012-09-07 | Extraction method for fructose from waste dextran fermentation broth |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153244A (en) * | 2015-08-04 | 2015-12-16 | 山东金朗生物科技有限公司 | Method for extracting sugar solution from waste liquid resulting from production of dextran macromolecule 1000 |
| CN109811090A (en) * | 2017-11-20 | 2019-05-28 | 问清江 | A kind of method that dextran-fermentation liquid prepares fructose syrup |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153244A (en) * | 2015-08-04 | 2015-12-16 | 山东金朗生物科技有限公司 | Method for extracting sugar solution from waste liquid resulting from production of dextran macromolecule 1000 |
| CN105153244B (en) * | 2015-08-04 | 2018-10-09 | 山东金朗生物科技有限公司 | A method of it is produced from dextran macromolecular 1000 and extracts sugar juice in waste liquid |
| CN109811090A (en) * | 2017-11-20 | 2019-05-28 | 问清江 | A kind of method that dextran-fermentation liquid prepares fructose syrup |
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Application publication date: 20130717 |