CN103197411B - Phase plate assembly and microscope system with field depth changeable - Google Patents
Phase plate assembly and microscope system with field depth changeable Download PDFInfo
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- CN103197411B CN103197411B CN201310089625.5A CN201310089625A CN103197411B CN 103197411 B CN103197411 B CN 103197411B CN 201310089625 A CN201310089625 A CN 201310089625A CN 103197411 B CN103197411 B CN 103197411B
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Abstract
The invention relates to the technical field of optics, and provides a phase plate assembly and a microscope system with field depth changeable. The phase plate assembly comprises at least two phase plates and a flat plate, wherein positions between the phase plates and the flat plate are changeable. The microscope system with the field depth changeable comprises a microscope, wherein the phase plate assembly is arranged on an aperture position of the microscope, and the positions between the phase plates and the flat plate ensure that one phase platen among the phase plates or the flat plate is located on the aperture position of the microscope. The field depth of the microscope system is changeable.
Description
Technical field
The present invention relates to the microscope system of optical technical field, more particularly to a kind of phase board component and variable depth of field
System.
Background technology
Microscope is a kind of instrument for amplifying small items, enabling the object to be visually noticeable.From the 17th century
Being developed so far occurs in the practical microscope of middle period First, and microscopical structure is continually changing.Traditional microscope is by optical section
Divide, lighting part and mechanical part are constituted, using human eye as receptor.Later camera head was added in microscope, with photosensitive
Film is used as the device that can record and store target image.The mid-80, with the development of digital industry and computer industry,
Microscopical function gets a promotion also by them, is substituted with photoelectric sensor, camera tube and charge-coupled image sensor photosensitive
Film, and in combination with laser, computer, information technology etc., constitute collection, record, process and the storage one of microcosmic signal
The system optical signal of change.
Microscopical application is quite varied, and different applications propose different requirements to microscopical performance.Remove
Outside to the requirement of its imaging resolution, in some application scenarios, such as medical treatment, biology, the structure of matter, integrated circuit neck
Domain, needs the three dimensional structure for studying small items, and this not only needs high-quality fault imaging, also requires that wider array of imaging model
Enclose, now require that microscope will not only have high magnification, and there is the big depth of field.The depth of field of conventional optical microscope is all very
It is little, when observation has the object of certain depth, blur-free imaging is just unable to, and with the microscopical depth of field of the raising of enlargement ratio
Can become less, which limits the range of application of conventional microscope.
Middle nineteen nineties in last century, professor Dowski of Colorado universities imaging system laboratory proposes wavefront volume
Code technology, i.e., be demodulated to expand the imaging technique of the optical system depth of field using optical modulation and image restoration technology.By ripple
It is simple for what the imaging of three-dimensional body just became after front coding techniques is applied in microscopic system, as long as carrying out suitably
Filtering, it is possible in the case where object structures are not destroyed to the observation thing blur-free imaging in the bigger depth of field.But, at present still
The microscopic system for not having variable depth of field occurs.
The content of the invention
A kind of drawbacks described above of the present invention for prior art, there is provided the microscope system of phase board component and variable depth of field
System, makes the microscopical depth of field variable.The present invention is adopted the following technical scheme that：
A kind of phase board component, the phase board component includes at least two phase boards and a flat board, the phase board
Position and flat board between is changeable.
Preferably, the phase board component also includes base plate and spill spin block, at least two phase board and a flat board
It is installed on spill spin block, connection is rotated between the spill spin block and base plate.
Preferably, the phase board is three phase boards, cosine form phase board or logarithmic function phase boards.
Preferably, the phase board is used to extend the microscope depth of field.
A kind of microscopic system of variable depth of field, the microscopic system includes microcobjective, in the microcobjective light
Late place is provided with above-mentioned phase board component, by switch the position between the phase board and flat board make one of phase board or
Person's flat board is located at microcobjective stop position.
Preferably, the microscopic system also include object stage, cylinder mirror, Amici prism, reflecting mirror, eyepiece, imaging lens,
Photoelectric receiving device, computer and display；The reflecting mirror and eyepiece are arranged in the light path all the way of Amici prism output, institute
State imaging lens and photoelectric receiving device to be arranged in another road light path of Amici prism output, the computer and display with
The outfan of photoelectric receiving device is connected.
The invention has the beneficial effects as follows：The phase board component of at least two phase boards and a flat board is included by design,
Position between phase board and flat board is changeable, when the phase board component is applied in microscopic system, by toggle bit phase
Position between plate and flat board makes one of phase board or flat board be located at microcobjective stop position, so as to realize microscope
The depth of field it is variable.
Description of the drawings
Fig. 1 is a kind of structural representation of the microscopic system of variable depth of field of the embodiment of the present invention；
Fig. 2 is a kind of partial structurtes design diagram of the microscopic system of variable depth of field of the embodiment of the present invention；
MTF curve when Fig. 3 is 0.63mm for conventional microscope operating distance；
Fig. 4 is MTF curve when adding the working distance of microscope after three phase boards to be 0.63mm；
MTF curve when Fig. 5 is 0.62mm for conventional microscope operating distance；
Fig. 6 is MTF curve when adding the working distance of microscope after three phase boards to be 0.62mm；
MTF curve when Fig. 7 is 0.61mm for conventional microscope operating distance；
Fig. 8 is MTF curve when adding the working distance of microscope after three phase boards to be 0.61mm.
Reference is as follows：
1- object stages, 2- microcobjectives,
3- phase board components, 3（1）- the first phase board,
3（2）- the second phase board, 3（3）- flat board,
3（4）- base plate, 3（5）- spill spin block,
4- cylinder mirrors, 5- Amici prisms,
6- reflecting mirrors, 7- eyepieces,
8- imaging lens, 9- photoelectric receiving devices,
10- computers, 11- display.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
Refer to shown in Fig. 1, embodiments provide a kind of microscopic system of variable depth of field, the microscopic system
Including object stage 1, microcobjective 2, cylinder mirror 4, Amici prism 5, reflecting mirror 6, eyepiece 7, imaging lens 8, photoelectric receiving device 9,
Computer 10 and display 11.Sample is placed with object stage 1, a phase board component 3 is provided with the diaphragm of microcobjective 2, should
Phase board component 3 includes at least two phase boards and a flat board, and the position between phase board and flat board is changeable, the present embodiment
In, phase board component 3 is provided with two phase boards and a flat board, is respectively the first phase board 3（1）, the second phase board 3（2）With
Flat board 3（3）, by switching the first phase board 3（1）, the second phase board 3（2）With flat board 3（3）Position between three makes wherein
One phase board or flat board 3（3）Positioned at the stop position of microcobjective 2.Specifically, it is provided with base plate 3 on the phase board component 3
（4）With spill spin block 3（5）, the first phase board 3（1）, the second phase board 3（2）With flat board 3（3）It is mounted on spill spin block 3（5）On,
Spill spin block 3（5）With base plate 3（4）Between rotate connection, by rotate spill spin block 3（5）I.e. changeable first phase board 3（1）,
Two phase boards 3（2）With flat board 3（3）Position between three, required phase board or flat board are rotated to the light of microcobjective 2
Late position.The form of phase board and parameter select have many kinds, can select three phase boards, cosine form phase boards or right
Number function phase board, what is selected in the present embodiment is three phase boards.Meanwhile, the first phase board 3（1）With the second phase board 3（2）
Can also according to demand change, for example, replace with the 3rd phase board, the 4th phase board etc..Reflecting mirror 6 and eyepiece 7 are arranged on and divide
In the light path all the way of the output of light prism 5, imaging lens 8 and photoelectric receiving device 9 are arranged on another road light that Amici prism 5 is exported
Lu Shang, computer 10 and display 11 are connected with the outfan of photoelectric receiving device 9.
The operation principle of the present embodiment is：The light that sample on microscope carrier 1 sends is through microcobjective 2 and cylinder
After mirror 4,5 points of Jing Amici prisms are two-way：All the way reflected mirror 6, eyepiece 7 reach human eye；Another road reaches through imaging lens 8
Photoelectric receiving device 9, and shown by display 11 after being processed by computer 10.Position phase is added in the stop position of microcobjective 2
After board component 3, the pupil function at the emergent pupil of microcobjective 2 is changed so that the modulation transfer function (MTF) of microscopic system
（Modulation Transfer Function, MTF）And point spread function（Point spread function, PSF）To thing
Away from insensitive, at this moment the intermediate image data for receiving is sent to computer 10 by photoelectric receiving device 9, carries out restoring filtering etc.
Shown by display 11 after process.Different phase boards or flat board 3 in by switching phase board component 3（3）, obtain difference
The microscope field depth of degree, the purpose variable so as to reach the microscope depth of field.When phase board component 3 switches to flat board 3（3）
When, the microscope depth of field is constant, uses as conventional microscope；When phase board component 3 switches to the first phase board 3（1）Or the
Two phase boards 3（2）When, the increase of the microscope depth of field, the first phase board 3（1）With the second phase board 3（2）Form and parameter according to
Practical application request is designed, and specific implementation refers to parameters described below cases of design.Need when phase board component 3 switches
The high positioning precision of comparison, it is ensured that the phase board or flat board 3 after switching（3）Positioned at the stop position of microcobjective 2.
Specifically parameter designing case：
Present case devises the microscopic system of three phase board components of an addition, using optical design software ZEMAX
Relative analyses have been carried out to the imaging characteristic of conventional microscope system and the microscopic system of the present embodiment, 10 times of depth of field have been given
In the range of modulation transfer function curve.Analog result shows, compared with conventional microscope system, this designed enforcement
The depth of field can be extended 10 times by the microscopic system of example in the case where luminous flux and image planes resolution is kept.Designed system is joined
Number is as follows：
N.A.=0.65,
M=-40 ×,
F '=4.649mm,
W.D.=0.63mm,
DOF=2.0μm。
Wherein, N.A. is numerical aperture, and M is amplification, and f ' is focal length, and W.D. is operating distance, and DOF is the depth of field.
Using three phase boards in the form of for z=α (x3+y3), wherein α is 0.005.First phase board, the second phase board
Adopt optical plastic polymethyl methacrylate（Polymethylmethacrylate, PMMA）Make, its thickness is 2mm.
The microscopic system partial structurtes design diagram of present case is as shown in Fig. 2 phase board component 3 is located at microcobjective diaphragm position
Put.
Through test, the microscopic system of conventional microscope system and the present embodiment offer has been investigated respectively in the different depth of field
The point spread function PSF and modulation transfer function situation of position, wherein MTF curve is as shown in Fig. 3-Fig. 8.From result of the test
Draw：Conventional microscope system with the depth of field increase, the rapid disperses of point spread function PSF, modulation transfer function drastically under
Drop；And add the microscopic system point spread function PSF after three phase board components that fixed form, modulation transfer function (MTF) is presented
MTF keeps certain value in 10 times of field depth, can recover final image by follow-up digital processing.Therefore,
The present embodiment by traditional microcobjective stop position add phase board component reached extension the microscope depth of field purpose, and
The depth of field is adjustable.
The embodiment of the present invention includes at least two by arranging phase board component, phase board component at microcobjective diaphragm
The phase board and a flat board of multi-form are planted, it can freely be switched over, the purpose variable so as to reach the microscope depth of field.
Embodiment of the present invention simple structure, low cost, on the premise of not reducing luminous flux, not focusing, solve conventional microscope scape
Deep little shortcoming, improves the adaptability that microscope applications are observed in the different depth of field, and may be selected there is provided multiple phase boards, scape
It is deep adjustable.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (1)
1. a kind of microscopic system of variable depth of field, it is characterised in that the microscopic system include object stage, microcobjective,
Cylinder mirror, Amici prism, reflecting mirror, eyepiece, imaging lens, photoelectric receiving device, computer and display, put on the object stage
Be equipped with sample, be provided with a phase board component at the microcobjective diaphragm, the phase board component be provided with two phase boards and
One flat board, described two phase boards are respectively the first phase board, the second phase board, the phase board component be provided with base plate and
Spill spin block, first phase board, the second phase board peace plate are mounted on spill spin block, the company of rotation between spill spin block and base plate
Connect, be the position between changeable first phase board, the second phase board peace plate three by rotating spill spin block, by required position
Phase-plate or flat board are rotated to the microcobjective stop position, and described two phase boards are three phase boards, three positions
The form of phase-plate is z=α (x3+y3), wherein α is 0.005, and described two phase boards adopt optical plastic poly-methyl methacrylate
Ester is made, and first phase board, the thickness of the second phase board are 2mm, and it is defeated that the reflecting mirror and eyepiece are arranged on Amici prism
In the light path all the way for going out, the imaging lens and photoelectric receiving device are arranged in another road light path of Amici prism output, institute
State computer and display to be connected with the outfan of the photoelectric receiving device；What the sample on the microscope carrier sent
After the microcobjective and cylinder mirror, Amici prism is divided into two-way to light described in Jing：All the way reflecting mirror, eyepiece are reached described in Jing
Human eye；Another road reaches the photoelectric receiving device through the imaging lens, and is carried out restoring filtering by the computer
Shown by the display after reason, wherein, in the phase board component of the microcobjective stop position, change described micro-
Pupil function at object lens emergent pupil, by the first phase board, the second phase board or flat board in switching phase board component, obtains not
With the microscope field depth of degree, the variable purpose of the microscope depth of field is realized, and when the phase board switch between components are to flat board
When, the microscope depth of field is constant, uses as conventional microscope；When the phase board switch between components to first phase board or
During second phase board, the increase of the microscope depth of field；Wherein, the numerical aperture of the microcobjective is N.A.=0.65, is amplified
Multiple M=-40 ×, focal length f '=4.649mm, operating distance W.D.=0.63mm, depth of field DOF=2.0 μm.
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| CN201310089625.5A CN103197411B (en) | 2013-03-20 | 2013-03-20 | Phase plate assembly and microscope system with field depth changeable |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104765137B (en) * | 2015-04-24 | 2017-09-08 | 合肥京东方光电科技有限公司 | Microlens and the microscopic system for including the microlens |
| CN106248692A (en) * | 2016-08-04 | 2016-12-21 | 中国科学院长春光学精密机械与物理研究所 | A kind of method of micro-imaging field depth extending and microscopic imaging device |
| CN106327435B (en) * | 2016-08-09 | 2019-02-01 | 中国科学院长春光学精密机械与物理研究所 | Image restoration imaging system and recovery system, image obtainment method and restored method |
| CN107622934B (en) * | 2017-09-22 | 2024-04-12 | 中国科学院生物物理研究所 | Phase plate replacement transfer device for transmission electron microscope imaging |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB682635A (en) * | 1949-09-02 | 1952-11-12 | Zeiss Opton Optische Werke | Phase-contrast microscope |
| EP2437096A2 (en) * | 2010-10-01 | 2012-04-04 | Carl Zeiss MicroImaging GmbH | Microscope and microscopy method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8537461B2 (en) * | 2007-11-26 | 2013-09-17 | Carl Zeiss Microimaging Gmbh | Method and configuration for the optical detection of an illuminated specimen |
| JP2009300881A (en) * | 2008-06-16 | 2009-12-24 | Olympus Corp | Phase-contrast microscope |
| CN102735609A (en) * | 2011-03-31 | 2012-10-17 | 西门子公司 | Body fluid imaging system, method and depth extended imaging device |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB682635A (en) * | 1949-09-02 | 1952-11-12 | Zeiss Opton Optische Werke | Phase-contrast microscope |
| EP2437096A2 (en) * | 2010-10-01 | 2012-04-04 | Carl Zeiss MicroImaging GmbH | Microscope and microscopy method |
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