CN103196814A - Method for improving repeatability of platelet counting detection of citric acid anticoagulant blood sample - Google Patents
Method for improving repeatability of platelet counting detection of citric acid anticoagulant blood sample Download PDFInfo
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- CN103196814A CN103196814A CN2012100065755A CN201210006575A CN103196814A CN 103196814 A CN103196814 A CN 103196814A CN 2012100065755 A CN2012100065755 A CN 2012100065755A CN 201210006575 A CN201210006575 A CN 201210006575A CN 103196814 A CN103196814 A CN 103196814A
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Abstract
The invention provides a method to ensure a citric acid anticoagulant blood sample to gain good repeatability in detection by a blood analyzer. The method provides by the invention can enable the citric acid anticoagulant blood sample to gain good platelet counting repeatability during counting detection on platelet of the blood sample by a blood analyzer and similar instrument.
Description
One, technical field
The present invention relates to a kind of method of improving citric acid anticoagulation sample of blood platelet number testing result repeatability, stability, belong to medical science vitro diagnostic techniques field.
Two, technical background
Platelet counts be clinical and related scientific research in the index that usually detects.Blood analyser under general room temperature condition to just obtaining better blood platelet testing result with the blood examination of EDTA anti-coagulants.Repeatability is relatively poor as a result and adopt citric acid anti-coagulants blood examination blood platelet under similarity condition, therefore at present general platelet count detect blood sample all with EDTA as anti-coagulants.And blood sample employing citric acid anti-coagulants necessary or preferably detects platelet counts in some experiments.The invention provides a kind of improved method, can make citric acid anti-freezing blood sample obtain the repeatability of good platelet count during to the platelet counts count detection at blood analyser and analogous instrument.This technology belongs to medical science vitro diagnostic techniques field.
Three, summary of the invention
Blood analyser adopts the EDTA anti-coagulants can obtain better blood platelet testing result under general room temperature condition.Repeatability is relatively poor as a result and adopt citric acid anti-coagulants blood examination blood platelet under similarity condition.In order to solve citric acid anticoagulation count results problem of unstable when blood analyser detects, we have invented a kind of new improving one's methods, and make the citric acid anticoagulated blood when blood analyser detects, and testing result is stable, repeatability is good.Can be applied to corresponding scientific research or clinical detection.Of the present invention to the effect that to the blood-sample withdrawal constant temperature measures of temperature within the specific limits, make the blood platelet of citric acid anti-freezing blood sample be in stable, be convenient to the state of count detection, thereby make citric acid anticoagulation sample when blood analyser detects, can obtain good repeatability, satisfy the needs of clinical and related scientific research.
Four, embodiment:
According to 9: 1 ratio (blood: the citric acid anti-coagulants) when newly collecting blood sample, just add 2.5% sodium citrate, make blood sample be able to anti-freezing.Before blood sample carries out count detection, blood sample was placed on 37 ℃ of incubator constant temperature 5-10 minute, make the blood sample temperature reach 37 ℃, with pipettor blood sample is inhaled repeatedly simultaneously at incubation and to be dashed, make blood sample obtain mixing in constant temperature, then the blood sample that this is heated is put into blood analyser and is detected.Sample all reaches the condition of 37 ℃ of constant temperature and mixing before each the detection, can obtain satisfied blood platelet and repeat numerical result.Subordinate list 1 is to detect two groups of blood platelet data results that obtain at same blood analyser and compare to adopt the inventive method to detect with adopting with the general mixing blood sample of not heating respectively with a citric acid blood sample.As can be seen, employing the inventive method detects the platelet counts that obtains, and repeatability, stability obviously are better than not adopting this method to detect the result of acquisition as a result from the result.
Table 1: adopt the inventive method not detect the platelet count of acquisition according to comparison sheet with adopting the inventive method
Data analysis and conclusion:
Adopt the inventive method and general conventional method that 20 same samples are detected, the inventive method detection acquisition platelet counts CV% value as a result is 1.77%.Be 12.42% and adopt the CV% value of the platelet counts that the citric acid blood examination of not heating obtains.The two difference is fairly obvious.Illustrate and adopt the inventive method to carry out the platelet counts testing result than the citric acid blood examination repeatability of not heating, stability better.
Claims (4)
1. one kind is improved the method that citric acid anticoagulation sample of blood platelet number detects repeatability, it is characterized in that: this method can improve platelet count detection stability, repeated in the blood sample, the characteristics of this method are that tested blood sample is guaranteed to heat before detection to the temperature conditions scope of 34-37.0 ± 0.5 ℃, namely require blood sample when detecting, should reach arbitrary temp point in the 34.0-37.0 ℃ of scope, and should control in ± 0.5 ℃ the blood sample temperature contrast in testing process;
2. a kind of citric acid anticoagulation sample of blood platelet number of improving according to claim 1 detects repeated method, it is characterized in that, this method be applicable to adopt electrical impedance method blood platelet is carried out the blood analyser of count detection, also be fit to the instrument that other similarly detects direct platelet count;
3. a kind of method that citric acid anticoagulation sample of blood platelet number detects repeatability of improving according to claim 1 is characterized in that this method also is applicable to the blood sample that other anti-coagulants is handled;
4. a kind of citric acid anticoagulation sample of blood platelet number of improving according to claim 1 detects repeated method, it is characterized in that this method can also be by further guaranteeing the blood platelet testing result stable to the heating of instrument agents useful for same, parts parts such as (as sample needle, pipelines), temperature control condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100065755A CN103196814A (en) | 2012-01-10 | 2012-01-10 | Method for improving repeatability of platelet counting detection of citric acid anticoagulant blood sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012100065755A CN103196814A (en) | 2012-01-10 | 2012-01-10 | Method for improving repeatability of platelet counting detection of citric acid anticoagulant blood sample |
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| CN103196814A true CN103196814A (en) | 2013-07-10 |
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| CN2012100065755A Pending CN103196814A (en) | 2012-01-10 | 2012-01-10 | Method for improving repeatability of platelet counting detection of citric acid anticoagulant blood sample |
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Citations (5)
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| US6200500B1 (en) * | 1999-08-20 | 2001-03-13 | Streck Laboratories, Inc. | Hematology control and system for multi-parameter hematology measurements |
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| CN1853688A (en) * | 2005-04-25 | 2006-11-01 | 北京天力正元医药技术开发有限公司 | Chinese medicinal preparation for treating heart cerebrovascular disease and ischemic apoplexia and making method thereof |
| CN101019905A (en) * | 2007-02-13 | 2007-08-22 | 南京凯腾科技有限公司 | Medicine composition containing tribulus fruit furostanol saponin and its application |
| CN101961376A (en) * | 2010-07-23 | 2011-02-02 | 新疆医科大学 | Method for refining sweet basil herb extract |
-
2012
- 2012-01-10 CN CN2012100065755A patent/CN103196814A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6200500B1 (en) * | 1999-08-20 | 2001-03-13 | Streck Laboratories, Inc. | Hematology control and system for multi-parameter hematology measurements |
| CN1404839A (en) * | 2002-10-28 | 2003-03-26 | 陈臻 | Notoginseng total saponin oral liquor |
| CN1853688A (en) * | 2005-04-25 | 2006-11-01 | 北京天力正元医药技术开发有限公司 | Chinese medicinal preparation for treating heart cerebrovascular disease and ischemic apoplexia and making method thereof |
| CN101019905A (en) * | 2007-02-13 | 2007-08-22 | 南京凯腾科技有限公司 | Medicine composition containing tribulus fruit furostanol saponin and its application |
| CN101961376A (en) * | 2010-07-23 | 2011-02-02 | 新疆医科大学 | Method for refining sweet basil herb extract |
Non-Patent Citations (2)
| Title |
|---|
| 彭黎明,等: "《血小板功能检测及其应用的研究进展》", 《中国实验诊断学》 * |
| 高健,等: "《夏天无总碱抑制血小板聚集作用的研究》", 《苏州大学学报( 医学版)》 * |
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Address after: 211135 Nanjing, Jiangning, Qilin street, Po Shan Road, No. 7 Applicant after: SINNOWA MEDICAL SCIENCE & TECHNOLOGY CO., LTD. Address before: 211135 Nanjing, Jiangning, Qilin street, Po Shan Road, No. 7 Applicant before: Nanjing Sinnowa Medical Science & Technology Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: NANJING SINNOWA MEDICAL SCIENCE + TECHNOLOGY CO., LTD. TO: JIANGSU SINNOWA MEDICAL SCIENCE + TECHNOLOGY CO., LTD. |
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Application publication date: 20130710 |