CN103194372B - Method and device for filtering and subpackaging microorganism culture medium - Google Patents
Method and device for filtering and subpackaging microorganism culture medium Download PDFInfo
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- CN103194372B CN103194372B CN201310101698.1A CN201310101698A CN103194372B CN 103194372 B CN103194372 B CN 103194372B CN 201310101698 A CN201310101698 A CN 201310101698A CN 103194372 B CN103194372 B CN 103194372B
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Abstract
The invention discloses a method and a device for filtering and subpackaging a microorganism culture medium. A filtering sieve is arranged at the upper part of a filtering cup body; a filtering gauze is put on the filtering sieve; an overflow nozzle positioned below the filtering sieve is arranged on the filtering cup body; the microorganism culture medium is poured from the top of the filtering cup body; after the microorganism culture medium is filtered by the filtering gauze, a filtrate deposits at the bottom of the filtering cup body; and the deposited filtrate is poured into a subpackaging container through the overflow nozzle, so that the microorganism culture medium are filtered and subpackaged at one time. According to the invention, filtering and subpackaging are integrated in the same filtering cup body, during filtering, the operation is simple, quick, time-saving and labor-saving; during subpackaging, a microorganism culture solution only needs to directly flow out from the overflow nozzle, so that the microorganism culture solution is not required to be poured into a funnel, the operation steps are reduced, and the microorganism culture solution is difficult to cool and concentrate. Therefore, a phenomenon of concentration of the filtrate caused of low speed during filling in the conventional subpackaging mode is avoided.
Description
Technical field
The present invention relates to a kind of filter method and device, particularly a kind of method and device that filters packing microbiological culture media.
Background technology
In the process of the substratum such as making in laboratory PDA, oat, rye, often need to be by the residue filter in nutrient solution, traditional method is while hot the substratum having prepared to be filtered with filter paper or gauze.While using filter paper filtering, folded filter paper is become to corrugated shape, be layered on funnel and filter, the method is simple to operate, but filtration velocity is very slow, and nutrient solution easily solidifies.While using filtered through gauze, be folded into 4～6 layers and be directly enclosed within beaker mouth, the method speed, but gauze is easily fallen into beaker while filtering, and filtration yield is when more, and a people is not easy to operate, often needs two people just can complete together.In the time that the nutrient solution after filtering is carried out to packing, conventional method is that nutrient solution is transferred in the funnel that is connected with spring pinchcock, and the degree of tightness of pressing from both sides by control spring transfers them to Erlenmeyer flask or test tube.Due to funnel finite capacity, if operated slow, the very fast cooled and solidified of nutrient solution.But, at present in laboratory, also do not collect substratum and filter and be packed as the easy device of one.
Summary of the invention
Technical problem to be solved by this invention is: a kind of method and device that filters packing microbiological culture media is provided, and it can disposable filtration and the packing that completes microbiological culture media, and have advantages of simple in structure, easy to use, time saving and energy saving.
The method of filtering packing microbiological culture media that is achieved in that of the present invention, arranges filter sieve on the top of filtering cup, and on filter sieve, places and filter gauze, and the mouth that overflows below filter sieve is set on filtration cup; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through filtering after filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in separation container by overflowing mouth again, thus the disposable filtration packing that completes microbiological culture media.
Filter the device of the method for packing microbiological culture media, comprise filtration cup, the inside upper part of filtering cup is provided with inwardly protruded planar rings, in filtration cup, be provided with filter sieve, the top of filter sieve is corresponding with planar rings, be provided with bowl cover at the top of filtering cup, be provided with one and overflow mouth on the sidewall that filters cup, the link position that overflows mouth and filter cup is in filter sieve below.
Be provided with handle overflowing on the position that mouth is corresponding.Easy to use.
Be provided with scale marks in the lower sidewall of filtering cup.The amount of nutrient solution in instruction cup, being convenient to measure, observing and adding water, it is volume required to supply.
On mouth, be provided with lid overflowing.In the time filtering, can close, in the time of packing, open.
Be provided with internal thread in the outside of filtering cup top, be provided with corresponding with it outside screw in the inner side of bowl cover.The mode being threaded can ensure to filter reliable, the good sealing effect of being connected of cup and bowl cover, and conveniently takes off.
Owing to having adopted technique scheme, compared with prior art, the present invention adopts and is integrated into the mode in same filtration cup by filtering with packing, simple to operate when filtration, quick, time saving and energy saving; When packing, only microbial culture medium directly need to be flowed out from overflowing mouth, not need again to proceed to funnel, reduce operation steps, and be difficult for cooled and solidified.The situation of having avoided existing packing mode to cause filtrate to be solidified slowly due to speed when filling.Method of the present invention is simple, and the device adopting is easily made, with low cost, and result of use is good.
Brief description of the drawings
Accompanying drawing 1 is structural representation of the present invention;
The structural representation that accompanying drawing 2 is filter sieve of the present invention.
Embodiment
Embodiments of the invention: filter the method for packing microbiological culture media, on the top of filtering cup, filter sieve is set, and place on filter sieve and filter gauze, the mouth that overflows below filter sieve is set on filtration cup; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through filtering after filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in separation container by overflowing mouth again, thus the disposable filtration packing that completes microbiological culture media.
The structure of the device of the method for filtration packing microbiological culture media as shown in Figure 1, comprise and filter cup 1, the inside upper part of filtering cup 1 is provided with inwardly protruded planar rings 7, in filtration cup 1, be provided with filter sieve 2, the top of filter sieve 2 is corresponding with planar rings 7, be provided with bowl cover 3 at the top of filtering cup 1, be provided with internal thread in the outside of filtering cup 1 top, be provided with corresponding with it outside screw in the inner side of bowl cover 3, on the sidewall that filters cup 1, be provided with one and overflow mouth 6, the link position that overflows mouth 6 and filter cup 1 is in filter sieve 2 belows; On the position of overflowing mouth 6 correspondences, be provided with handle 5; Be provided with scale marks 4 in the lower sidewall of filtering cup 1; Be provided with lid overflowing on mouth 6.
In use, take off bowl cover 3, and on filter sieve 2, lay 4-6 layer gauze, the nutrient solution such as PDA or OA is poured on gauze and is filtered, after filtration completes, take out filter sieve 2, add water as required that it is volume required to supply, then tighten bowl cover 3, open the lid that overflows mouth 6, hold handle 5, pour nutrient solution into Erlenmeyer flask by overflowing mouth 6, if need test tube slant, connect a rubber hose with spring pinchcock overflowing mouth, pinch loose spring pinchcock, make nutrient solution flow into test tube, can avoid so filling time, speed is solidified slowly; While filtration, cup can be done the use of volume measuring cup.
The material selection stainless material or the high-temperature resistance plastice that filter cup 1, its external form is right cylinder, and volume is about 3000～4000mL, and concrete size, shape can design according to demand.
Claims (6)
1. a method of filtering packing microbiological culture media, is characterized in that: on the top of filtering cup, filter sieve is set, and on filter sieve, places and filter gauze, the mouth that overflows below filter sieve is set on filtration cup; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through filtering after filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in separation container by overflowing mouth again, thus the disposable filtration packing that completes microbiological culture media.
2. one kind adopts the device of the method for filtration packing microbiological culture media as claimed in claim 1, comprise and filter cup (1), it is characterized in that: the inside upper part of filtering cup (1) is provided with inwardly protruded planar rings (7), in filtration cup (1), be provided with filter sieve (2), the top of filter sieve (2) is corresponding with planar rings (7), be provided with bowl cover (3) at the top of filtering cup (1), on the sidewall that filters cup (1), be provided with one and overflow mouth (6), the link position that overflows mouth (6) and filter cup (1) is in filter sieve (2) below.
3. the device of filtration packing microbiological culture media according to claim 2, is characterized in that: on the position of overflowing mouth (6) correspondence, be provided with handle (5).
4. the device of filtration packing microbiological culture media according to claim 2, is characterized in that: be provided with scale marks (4) in the lower sidewall of filtering cup (1).
5. the device of filtration packing microbiological culture media according to claim 2, is characterized in that: be provided with lid overflowing on mouth (6).
6. the device of filtration packing microbiological culture media according to claim 2, is characterized in that: be provided with internal thread in the outside of filtering cup (1) top, be provided with corresponding with it outside screw in the inner side of bowl cover (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310101698.1A CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310101698.1A CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103194372A CN103194372A (en) | 2013-07-10 |
| CN103194372B true CN103194372B (en) | 2014-07-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310101698.1A Expired - Fee Related CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670906B (en) * | 2016-03-02 | 2018-01-16 | 王皓宇 | Hand is incubated packaging device from dual-purpose type microbiological culture media |
| CN109603245A (en) * | 2019-01-08 | 2019-04-12 | 贵州大学 | A kind of tissue culture filter device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200999248Y (en) * | 2007-01-19 | 2008-01-02 | 叶大林 | Micro-organism limited filtration incubator |
| CN201241126Y (en) * | 2008-05-10 | 2009-05-20 | 樊笑梅 | Disposable microorganism test board |
| CN201962275U (en) * | 2010-12-09 | 2011-09-07 | 中国热带农业科学院海口实验站 | Device for culturing rare microorganisms |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN203154904U (en) * | 2013-03-27 | 2013-08-28 | 贵州大学 | Microorganism culture medium filter device |
-
2013
- 2013-03-27 CN CN201310101698.1A patent/CN103194372B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200999248Y (en) * | 2007-01-19 | 2008-01-02 | 叶大林 | Micro-organism limited filtration incubator |
| CN201241126Y (en) * | 2008-05-10 | 2009-05-20 | 樊笑梅 | Disposable microorganism test board |
| CN201962275U (en) * | 2010-12-09 | 2011-09-07 | 中国热带农业科学院海口实验站 | Device for culturing rare microorganisms |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN203154904U (en) * | 2013-03-27 | 2013-08-28 | 贵州大学 | Microorganism culture medium filter device |
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| CN103194372A (en) | 2013-07-10 |
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