CN103194372A - Method and device for filtering and subpackaging microorganism culture medium - Google Patents
Method and device for filtering and subpackaging microorganism culture medium Download PDFInfo
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- CN103194372A CN103194372A CN2013101016981A CN201310101698A CN103194372A CN 103194372 A CN103194372 A CN 103194372A CN 2013101016981 A CN2013101016981 A CN 2013101016981A CN 201310101698 A CN201310101698 A CN 201310101698A CN 103194372 A CN103194372 A CN 103194372A
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Abstract
The invention discloses a method and a device for filtering and subpackaging a microorganism culture medium. A filtering sieve is arranged at the upper part of a filtering cup body; a filtering gauze is put on the filtering sieve; an overflow nozzle positioned below the filtering sieve is arranged on the filtering cup body; the microorganism culture medium is poured from the top of the filtering cup body; after the microorganism culture medium is filtered by the filtering gauze, a filtrate deposits at the bottom of the filtering cup body; and the deposited filtrate is poured into a subpackaging container through the overflow nozzle, so that the microorganism culture medium are filtered and subpackaged at one time. According to the invention, filtering and subpackaging are integrated in the same filtering cup body, during filtering, the operation is simple, quick, time-saving and labor-saving; during subpackaging, a microorganism culture solution only needs to directly flow out from the overflow nozzle, so that the microorganism culture solution is not required to be poured into a funnel, the operation steps are reduced, and the microorganism culture solution is difficult to cool and concentrate. Therefore, a phenomenon of concentration of the filtrate caused of low speed during filling in the conventional subpackaging mode is avoided.
Description
Technical field
The present invention relates to a kind of filter method and device, particularly a kind of method and device that filters the packing microbiological culture media.
Background technology
In the process of substratum such as making in laboratory PDA, oat, rye, often the residue in the nutrient solution need be filtered, traditional method is to filter with the substratum that filter paper or gauze will prepare while hot.When using filter paper filtering, folded filter paper is become corrugated shape, be layered on the funnel and filter, this method is simple to operate, but filtration velocity is very slow, and nutrient solution solidifies easily.When using filtered through gauze, it is folded into 4～6 layers directly is enclosed within the beaker mouth, this method speed is very fast, but gauze is easily fallen into beaker when filtering, and filtration yield more for a long time, and a people is not easy to operate, often needs two people just can finish together.When the nutrient solution after filtering was carried out packing, method commonly used was that nutrient solution is transferred in the funnel that is connected with spring pinchcock, and the degree of tightness of pressing from both sides by control spring is transferred to Erlenmeyer flask or test tube with it.Because the funnel finite capacity is slow as if operating, the very fast cooled and solidified of nutrient solution.Yet, also do not collect the easy device that substratum filtered and be packed as one in the laboratory at present.
Summary of the invention
Technical problem to be solved by this invention is: a kind of method and device that filters the packing microbiological culture media is provided, and disposable filtration and the packing of finishing microbiological culture media of its energy, and have simple in structure, easy to use, time saving and energy saving advantage.
The method of filtering the packing microbiological culture media that is achieved in that of the present invention arranges filter sieve on the top of filtering cup, and places at filter sieve and to filter gauze, is in the mouth that overflows below the filter sieve filtering cup setting; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through after filtering filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in the branch packaging container by overflowing mouth again, thus the disposable filtration packing of finishing microbiological culture media.
Filter the device of the method for packing microbiological culture media, comprise the filtration cup, the inside upper part of filtering cup is provided with inwardly protruded planar rings, in filtering cup, be provided with filter sieve, the top of filter sieve is corresponding with planar rings, be provided with bowl cover at the top of filtering cup, be provided with one at the sidewall that filters cup and overflow mouth, overflow mouth and be in the filter sieve below with the link position that filters cup.
Be provided with handle in the position of overflowing the mouth correspondence.The convenient use.
This has scale marks in the lower sidewall of filtering cup.The amount of nutrient solution is convenient to measure, observes and is added water and supplies volume required in the indication cup.
Be provided with lid overflowing mouth.When filtering, can close, when packing, open.
Be provided with internal thread in the outside of filtering the cup top, be provided with corresponding with it outside screw in the inboard of bowl cover.The mode that is threaded can guarantee to filter reliable, the good sealing effect of being connected of cup and bowl cover, and conveniently takes off.
Owing to adopted technique scheme, compared with prior art, the present invention adopts and will filter and packing is integrated into mode in the same filtration cup, and is simple to operate during filtration, quick, time saving and energy saving; During packing, only microbial culture medium directly need be got final product from overflowing the mouth outflow, not need to change over to again funnel, reduce operation steps, and be difficult for cooled and solidified.The situation of having avoided existing packing mode when can, to cause filtrate to be solidified slowly owing to speed.Method of the present invention is simple, and the device that adopts is made easily, and is with low cost, and result of use is good.
Description of drawings
Accompanying drawing 1 is structural representation of the present invention;
Accompanying drawing 2 is the structural representation of filter sieve of the present invention.
Embodiment
Embodiments of the invention: filter the method for packing microbiological culture media, on the top of filtering cup filter sieve is set, and place at filter sieve and to filter gauze, be in the mouth that overflows below the filter sieve filtering cup setting; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through after filtering filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in the branch packaging container by overflowing mouth again, thus the disposable filtration packing of finishing microbiological culture media.
The structure of the device of the method for filtration packing microbiological culture media as shown in Figure 1, comprise and filter cup 1, the inside upper part of filtering cup 1 is provided with inwardly protruded planar rings 7, in filtering cup 1, be provided with filter sieve 2, the top of filter sieve 2 is corresponding with planar rings 7, be provided with bowl cover 3 at the top of filtering cup 1, be provided with internal thread in the outside of filtering cup 1 top, be provided with corresponding with it outside screw in the inboard of bowl cover 3, be provided with one at the sidewall that filters cup 1 and overflow mouth 6, overflow mouth 6 and be in filter sieve 2 belows with the link position that filters cup 1; Be provided with handle 5 in the position of overflowing mouth 6 correspondences; This has scale marks 4 in the lower sidewall of filtering cup 1; Be provided with lid overflowing mouth 6.
In use, take off bowl cover 3, and at filter sieve 2 laying 4-6 layer gauze, nutrient solutions such as PDA or OA are poured on filter on the gauze, after filtration is finished, take out filter sieve 2, add water as required and supply volume requiredly, tighten bowl cover 3 then, open the lid that overflows mouth 6, hold handle 5, pour nutrient solution into Erlenmeyer flask by overflowing mouth 6, if need the test tube slant, then overflowing the rubber hose that mouth connects a band spring pinchcock, pinch loose spring pinchcock, make nutrient solution flow into test tube and get final product, speed is solidified slowly in the time of can avoiding can like this; Cup can be done the use of volume measuring cup when not filtering.
Filter material selection stainless material or the high-temperature resistance plastice of cup 1, its external form is right cylinder, and volume is about 3000～4000mL, and concrete size, shape can design according to demand.
Claims (6)
1. method of filtering the packing microbiological culture media is characterized in that: on the top of filtering cup filter sieve is set, and places at filter sieve and to filter gauze, be in the mouth that overflows below the filter sieve filtering cup setting; Microbiological culture media is poured into from the top of filtering cup, microbiological culture media is through after filtering filtered through gauze, filtrate is deposited on the bottom of filtering cup, and the filtrate of deposition is poured in the branch packaging container by overflowing mouth again, thus the disposable filtration packing of finishing microbiological culture media.
2. device that adopts the method for filtration packing microbiological culture media as claimed in claim 1, comprise and filter cup (1), it is characterized in that: the inside upper part of filtering cup (1) is provided with inwardly protruded planar rings (7), in filtering cup (1), be provided with filter sieve (2), the top of filter sieve (2) is corresponding with planar rings (7), be provided with bowl cover (3) at the top of filtering cup (1), be provided with one at the sidewall that filters cup (1) and overflow mouth (6), overflow mouth (6) and be in filter sieve (2) below with the link position that filters cup (1).
3. the device of filtration packing microbiological culture media according to claim 2 is characterized in that: be provided with handle (5) in the position of overflowing mouth (6) correspondence.
4. the device of filtration packing microbiological culture media according to claim 2 is characterized in that: this has scale marks (4) in the lower sidewall of filtering cup (1).
5. the device of filtration packing microbiological culture media according to claim 2 is characterized in that: be provided with lid overflowing mouth (6).
6. the device of filtration packing microbiological culture media according to claim 2 is characterized in that: be provided with internal thread in the outside of filtering cup (1) top, be provided with corresponding with it outside screw in the inboard of bowl cover (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310101698.1A CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310101698.1A CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103194372A true CN103194372A (en) | 2013-07-10 |
| CN103194372B CN103194372B (en) | 2014-07-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310101698.1A Expired - Fee Related CN103194372B (en) | 2013-03-27 | 2013-03-27 | Method and device for filtering and subpackaging microorganism culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670906A (en) * | 2016-03-02 | 2016-06-15 | 王皓宇 | Manual and automatic dual-purpose type microorganism culture medium thermal insulation subpackage device |
| CN109603245A (en) * | 2019-01-08 | 2019-04-12 | 贵州大学 | A kind of tissue culture filter device |
Citations (5)
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| CN200999248Y (en) * | 2007-01-19 | 2008-01-02 | 叶大林 | Micro-organism limited filtration incubator |
| CN201241126Y (en) * | 2008-05-10 | 2009-05-20 | 樊笑梅 | Disposable microorganism test board |
| CN201962275U (en) * | 2010-12-09 | 2011-09-07 | 中国热带农业科学院海口实验站 | Device for culturing rare microorganisms |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN203154904U (en) * | 2013-03-27 | 2013-08-28 | 贵州大学 | Microorganism culture medium filter device |
-
2013
- 2013-03-27 CN CN201310101698.1A patent/CN103194372B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200999248Y (en) * | 2007-01-19 | 2008-01-02 | 叶大林 | Micro-organism limited filtration incubator |
| CN201241126Y (en) * | 2008-05-10 | 2009-05-20 | 樊笑梅 | Disposable microorganism test board |
| CN201962275U (en) * | 2010-12-09 | 2011-09-07 | 中国热带农业科学院海口实验站 | Device for culturing rare microorganisms |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN203154904U (en) * | 2013-03-27 | 2013-08-28 | 贵州大学 | Microorganism culture medium filter device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670906A (en) * | 2016-03-02 | 2016-06-15 | 王皓宇 | Manual and automatic dual-purpose type microorganism culture medium thermal insulation subpackage device |
| CN109603245A (en) * | 2019-01-08 | 2019-04-12 | 贵州大学 | A kind of tissue culture filter device |
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| Publication number | Publication date |
|---|---|
| CN103194372B (en) | 2014-07-16 |
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Granted publication date: 20140716 Termination date: 20190327 |