CN103190886A - Novel optical method detection system for biological tissue pathological changes - Google Patents
Novel optical method detection system for biological tissue pathological changes Download PDFInfo
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- CN103190886A CN103190886A CN2013101023171A CN201310102317A CN103190886A CN 103190886 A CN103190886 A CN 103190886A CN 2013101023171 A CN2013101023171 A CN 2013101023171A CN 201310102317 A CN201310102317 A CN 201310102317A CN 103190886 A CN103190886 A CN 103190886A
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Abstract
The invention relates to a novel optical method detection system for biological tissue pathological changes. According to the novel optical method detection system, based on analyzing non-uniform polarization structures of fields, which are scatted from biological tissues, pathological changes of birefringence biological tissues are detected through measurement of spatial distribution of field parameters of biological tissue images.
Description
Technical field
The present invention relates to a kind of optical system of utilizing the singular point method of optics to come the detection of biological lesion tissue.The present invention utilizes coherent laser to obtain polarization diagram as the result of relational structure by biological tissue, and the measuring technique of utilizing the coordinate distribution of mutual degree of polarization (DMP), come polarize inhomogeneous image of biological tissue is made topology and described, the result shows that the half-breadth of auto-correlation function of the coordinate distribution of S profile and DMP value has relation.These information can become the basis of clinical diagnosis biological tissue canceration.
Background technology
Now, medical science is in a great change period.The emphasis of medical science is just by traditional changing to the treatment pattern that with information is foundation based on the symptom treatment pattern.It has been recognized that symptom only is the very coarse human body abnormal response that disease is lagged behind.More current significant medical important function for of research are placed on exploration to the starting point at the very start and cause on the bio information rule of disease, are in health status with control bio-logic information, and then reach the purpose for the treatment of disease.For this reason, people explore the new method of medical diagnosis and treatment from each subject (magnetics, acoustics, chemistry, optics etc.).At present, it is believed that optics is hopeful to play an important role in the great change of current medical science.The understanding propagation law of light in biological tissue, and laser is that high-performance light sources and the succeeding in developing of high sensitivity optical detector of representative is respectively theoretical foundation and the material base of this cognition.Many advantages though traditional optical detection and diagnosis have been compared with the method for traditional medicine, especially Non-Destructive Testing and the diagnostic techniques in 600nm to 1300nm " optical window " wave-length coverage is flourish, as the monitoring of detection, blood oxygen and the glucose content of tissue oxygenation and brain blood oxygen.The OCT technology that developed recently gets up aspect imaging technique also is subjected to people's great attention, but because multiformity and the complexity of biological tissue, traditional optical detects and in theory especially how diagnostic techniques still has many problems need strengthen research for clinical medicine provides reliable physiological parameter index, and the effectiveness and the reliability that detect and treat also have perfect space.The present invention is on the basis of noncontact, unionized, no wound or Wicresoft's wound and the high flexibility advantage of inheriting tradition optical detection pathological changes biological tissue, utilize the singular point optical means to detect pathologic pathological changes birefringence biological tissue, thereby can provide biological tissue's body field parameter more accurately for clinical medicine.
Summary of the invention
In order to improve the reliability of organism optical parameter, we have proposed a kind of novel optical method detection system.This system is different with the thinking that traditional optical detects, combine the field structure characteristics of singular point optics uniqueness, based on the non-homogeneous polarization structure of analyzing the field that scatters out from biological tissue, singular point polarization by measuring biological tissue's image and the spatial distribution of field parameter such as degree of polarization (DMP value) mutually detect the pathologic pathological changes of birefringence biological tissue.
Technical scheme of the present invention is: measuring system as shown in Figure 1, light source is the He-Ne Lasers that has collimated, (
λ=0.6328 μ m,
W=5.0 mw, diameter are 10 mm).Polarization is that the polarizer 4 is adjusted angle and the ellipticity of polarized lights by the control of 3 and 5 quarter-wave plate, wherein 0≤
α 0≤ 180
0, 0≤
β 0≤ 90
0The polarization diagram of biological tissue similarly is to be transmitted into ccd array 9 with microobject 7 to obtain, and the pixel of CCD is 800*600, and ccd array can be measured for biological tissue provides from 2 to 200 microns side scope.Can analyze biological tissue's image with the polarizer 8.
The present invention compares the field structure characteristics that can utilize singular point optics uniqueness with other optical detecting method, and the polarization parameter space of the field that acquisition biological tissue scatters out distributes, thereby detects the pathologic pathological changes of birefringence biological tissue.The present invention is simple in structure, is easy to make.
The present invention is further described below in conjunction with accompanying drawing.
Fig. 1 is the novel optical method detection system sketch map of biological tissue's pathological changes.
Fig. 2 is the research object among the embodiment one.
Fig. 3 is the experimental result among the embodiment one.
Fig. 4 is the research object among the embodiment two.
Fig. 5 is the experimental result one among the embodiment two.
Fig. 6 is the experimental result two among the embodiment two.
Fig. 7 is the experimental result three among the embodiment two.
Fig. 8 is the experimental result four among the embodiment two.
1 helium neon laser among Fig. 1,2 collimators, 3,5 quarter-wave plates, 4,8 polarizers, the object of 6 researchs, 7 microobjects, 9CCD camera, 10 processing units.
Cardiac muscular tissue's polarization diagram picture that 1 usefulness is coaxial and the 2 intersection polarizers obtain among Fig. 2." P " labelling be to be studied polarization topological structure zone.
The topology distribution of cardiac muscular tissue's image polarization singular point (S profile) among Fig. 3.S state stain labelling; The C point of dextrorotation is with red some labelling, left-handed C point Bluepoint labelling.
The dermal tissue of normal and 2 cancerations of 1 physiology is at the polarization diagram picture that intersects under the polarizer among Fig. 4.
The polarization ellipse rate of the dermal tissue image of normal and 2 cancerations of 1 physiology among Fig. 5
β(
r) coordinate distribute.
Single degeneracy of the dermal tissue of normal and 2 cancerations of 1 physiology and the coordinate of two degeneracys polarization singular points distribute among Fig. 6.
The DMP value distribution of the dermal tissue of normal and 2 cancerations of 1 physiology among Fig. 7.
The two-dimensional autocorrelation function that the DMP coordinate of the dermal tissue of 1 normal and 2 cancerations distributes among Fig. 8.The graphics of the auto-correlation function of the DMP correspondence of the dermal tissue of 3 normal and 4 cancerations.
Specific embodiment:
In embodiment shown in Figure 2, we selected a geometric thickness be 100 microns cardiac muscular tissue as object of study, the topological structure of the polarization non-uniform image that obtains by computer model and experiment is analyzed.What Fig. 3 showed is the topology distribution of S type and C type polarization singular point, this be the P zone of cardiac muscular tissue (see figure 2) the state of light source for
α 0=0
0 β 0=0
0Situation under experiment obtain.The result shows that all types of S profiles all occur in the polarization diagram picture of cardiac muscular tissue, with the similar of all S profile of the birefringence biological tissue of reality.
In embodiment shown in Figure 42, we have studied the dermal tissue of normal and canceration.1 is the micro-image of the corium polarization visual structure that obtains with cross polarization light among Fig. 4.The 2nd, the dermal tissue of canceration, it is accompanied by physical dimension and becomes big collagen protein.It is the ellipticity of the dermal tissue of 1 normal and 2 cancerations among Fig. 5
β(
r) the coordinate scattergram.The result shows that the boundary field of two kinds of dermal tissue samples all is that polarization is inhomogeneous.The main feature of the image polarization structure of the dermal tissue of canceration is
β(
r) physical dimension become big.The birefringence collagen structure of this and increased in size has relation.We have listed the coordinate distribution of polarization singular point in Fig. 6, and the point of green (representing the linear polarization attitude) and red (representing left-handed and polarization state dextrorotation) is stacked on the corresponding corium image.Fig. 7 is the coordinate distribution of 1 dermal tissue image calculation normal and 2 cancerations the DMP value of coming out.Fig. 8 has described the auto-correlation function of deriving from 1 dermal tissue DMP coordinate distributed images normal and 2 cancerations
G(| V
2|).The half-breadth of the auto-correlation function of two kinds of dermal tissues of the tangible demonstration of data and the size of S profile all approach.
Claims (2)
1. novel optical method detection system that can be used for detecting pathologic pathological changes biological tissue, by helium neon laser, collimator, quarter-wave plate, the polarizer, the object of research, microobject, composition such as CCD camera and computer processing unit is characterized in that: can utilize this system to obtain the polarization field of biological tissue, thereby measure biological tissue's image.
2. the polarizer 8 according to claim 1 is characterized in that: insert the polarizer 8 in system after, the angle of transmission axle is adjusted into Θ=0 successively
0, Θ=90
0, measure light intensity respectively
I (0)(
r m , n ),
I (90)(
r m , n ), then in Θ=0
0→ 180
0Scope in change the direction of the polarizer 8 transmission axles, measure the minimum and maximum light intensity that each pixel receives
I Max (
r m , n ) and
I Min (
r m , n ), the rotational angle Θ of measurement correspondence simultaneously (
r m,
n ) |
I(r
m,
n)=
IMin
, the coordinate that utilizes measurement data to calculate the light vibration polarization state of biological tissue then distributes and the DMP value of biological tissue's image.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050240107A1 (en) * | 2003-04-17 | 2005-10-27 | Research Foundation Of The City University Of New York | Detecting human cancer through spectral optical imaging using key water absorption wavelengths |
| CN101371129A (en) * | 2006-01-19 | 2009-02-18 | 香港中文大学 | Surface plasmon resonance sensors and methods for detecting samples using the same |
| US20090131800A1 (en) * | 2007-11-15 | 2009-05-21 | Carestream Health, Inc. | Multimodal imaging system for tissue imaging |
| CN102183466A (en) * | 2011-02-22 | 2011-09-14 | 复旦大学 | Time resolution elliptical polarization spectrum measuring system |
-
2013
- 2013-03-28 CN CN2013101023171A patent/CN103190886A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050240107A1 (en) * | 2003-04-17 | 2005-10-27 | Research Foundation Of The City University Of New York | Detecting human cancer through spectral optical imaging using key water absorption wavelengths |
| CN101371129A (en) * | 2006-01-19 | 2009-02-18 | 香港中文大学 | Surface plasmon resonance sensors and methods for detecting samples using the same |
| US20090131800A1 (en) * | 2007-11-15 | 2009-05-21 | Carestream Health, Inc. | Multimodal imaging system for tissue imaging |
| CN102183466A (en) * | 2011-02-22 | 2011-09-14 | 复旦大学 | Time resolution elliptical polarization spectrum measuring system |
Non-Patent Citations (5)
| Title |
|---|
| J.F.NYE: "Lines of circular polarization in electromagnetic wave fields", 《PRO. R. SOC. LOND. A》, 8 October 1983 (1983-10-08), pages 279 - 290 * |
| M.V BEERY 等: "Polarization singularities in isotropic random vector waves", 《PRO. R. SOC. LOND. A》, 8 January 2001 (2001-01-08), pages 141 - 155 * |
| O. V. ANGELSKY 等: "Polarization-correlation mapping of biological tissue coherent images", 《JOURNAL OF BIOMEDICAL OPTICS》, vol. 10, no. 1, 5 January 2006 (2006-01-05), pages 1 - 12 * |
| 邓凯 等: "奇点光学法诊断生物组织病理性病变", 《湖南师范大学自然科学学报》, vol. 34, no. 1, 27 February 2011 (2011-02-27), pages 27 - 36 * |
| 邓凯: "病理性病变生物组织的光学方法检测", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》, no. 12, 15 December 2011 (2011-12-15), pages 49 - 50 * |
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Application publication date: 20130710 |