CN103181957A - Preparation technology capable of completely removing acute toxicity of prepared aconite roots and keeping pharmacological activity of prepared aconite roots - Google Patents
Preparation technology capable of completely removing acute toxicity of prepared aconite roots and keeping pharmacological activity of prepared aconite roots Download PDFInfo
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Abstract
The invention provides a preparation technology capable of completely removing the acute toxicity of prepared aconite roots and keeping the pharmacological activity of the prepared aconite roots. The technology comprises the following steps: firstly, selecting medium-sized crude aconite roots, washing the crude aconite roots and then weighing the crude aconite roots, adding 25 L of edible brine and 12.5 L of fresh water into each 50 kg of the aconite roots to soak the aconite roots for 7 days, secondly, laying the aconite roots into fresh water to soak the aconite roots for 24 hours after boiling out the aconite roots for 1 hour, peeling crusts of the aconite roots, then soaking the aconite roots for 10 hours again, thirdly, longitudinally cutting the aconite roots into 2 to 3 mm slices, laying the slices into fresh air to soak the slices for 10 hours, drying the slices till folded corners appear, drying or baking the slices till the slices are completely dried after fuming the slices with sulphur, fourthly, adding the slices into water with the volume being 5 to 10 times of that of the slices to soak the slices for 30 minutes, then boiling the slices, decocting the slices for 2 hours in an open way, and fifthly, taking supernatant through centrifugation, then obtaining non-toxic aconite root liquor, and using the non-toxic aconite root liquor after concentration. The preparation technology has the main benefits that the method is simple and stable, the cost is low, aconite roots can be ensured to be completely detoxicated, a guarantee is provided for the clinical safe application of aconite roots, and good economic and social benefits can be expected to produce.
Description
Technical field
The present invention relates to Chinese medicine Radix Aconiti Lateralis Preparata field, be specifically related to a kind of preparation technology that can remove the Radix Aconiti Lateralis Preparata acute toxicity fully and keep its drug activity.
Background technology
Radix Aconiti Lateralis Preparata is the daughter root of cohosh Aconitum carmichjaelii Debx. (Aconi tum carmichaeli Debx), the little suffering of property, big heat, poisonous, and GUIXIN, kidney, spleen channel have recuperating depleted YANG and rescuing the patient from collapse, mend effect fiery supporing yang, dispersing cold for relieving pain, are described as " the recuperating depleted YANG and rescuing the patient from collapse first product medicine ".Forefathers are called the panacea in the medicine, are used for urgency, danger, serious symptom, and multipotency is made vigorous efforts to turn the tide; Impose on chronic stupid sick obstinate disease, also can play severe and lingering illness in the moment.It is clinical that the peculiar effect of Radix Aconiti Lateralis Preparata is widely used in it always.Yet this plant Herb is poisonous, and is the most malicious with the root head.The toxic ingredient of Radix Aconiti Lateralis Preparata is aconitine (Aconitine), the water-soluble and ethanol of energy, and toxicity is very big, and toxic reaction can take place in human oral 0.2mg aconitine, and 3～5mg can cause death, so classify low-grades as in Shennong's Herbal." poison " of Radix Aconiti Lateralis Preparata and " effect " are also deposited, virose one side, the one side that therapeutic efficiency is arranged again, with it improper toxic and side effects that takes place, and correct the use often produces quick-actingly, efficient, treats a lot of danger and disaster serious symptoms, so some doctor families are called the medicine of tiger and wolf and dare not use, but some well-known doctors then are called specific drug, and pertinacious disease obstinate disease multipotency answers hands to get effect.1 piece of Radix Aconiti Lateralis of Eastern Han Dynasty's Zhang Zhongjing, 1～3 piece of gun additioner is roughly equal to 15g and does not wait to 60g.Modern age, wear weighing apparatus, Liu Minshu, Li Jichang of old docter of TCM Wu was also big with the Radix Aconiti Lateralis Preparata amount, general all more than 30g.Wu Peiheng even single agent consumption 500g, no toxicity, evident in efficacy on the contrary.But, it is reported have the people once to obey Radix Aconiti Lateralis Preparata 9g and poison.Therefore, there is potential risk all the time in the clinical practice of Radix Aconiti Lateralis Preparata, and Radix Aconiti Lateralis Preparata toxicity is the problem of clinical primary solution.
Modern pharmacological research shows that Radix Aconiti Lateralis Preparata has effects such as antiinflammatory, analgesia, adjusting immunity, and in the clinical diseases such as treatment of arthritis, cervical spondylosis, prolapse of lumbar intervertebral disc, scapulohumeral periarthritis, ankylosing spondylitis that are usually used in of orthopaedics, drug effect is remarkable.Toxicity and the active ingredient of Radix Aconiti Lateralis Preparata are closely related, and its material base mainly is diterpene di esters alkaloid, comprise aconitine (aconitine), mesaconitine (mesaconitine) and hypaconitine (hypaconitine).Aconite alkaloids toxicity is very strong, easily neural and cardiovascular system is caused serious injury, and causes arrhythmia, ventricular tachycardia, ventricular fibrillation, systematicness paralysis, nauseating, vomiting, dizzy, shock and stupor etc.Simultaneously, the aconitine composition has very strong anti-inflammatory and antalgic activity again, can significantly suppress the rat paw edema that multiple proinflammatory agents such as carrageenin, Ovum Gallus domesticus album, histamine and 5-HT cause, obviously suppressing the capillary permeability that the swollen and histamine of mice caused by dimethylbenzene xylene ear, 5-HT cause increases, and reduces leukocytic oozing out in the inflammatory exudate.Nowadays aconitine is poisoned becomes the scruple of the maximum of Radix Aconiti Lateralis Preparata clinical practice.How bringing into play drug action to greatest extent and produce minimal side effects, is a great problem for Radix Aconiti Lateralis Preparata.
From ancient times to the present, doctor and scholar pursue " attenuation synergistic " of Radix Aconiti Lateralis Preparata always, have proposed attenuation strategies such as different concocting methods, various dose and different compatibilities.Many with Radix Glycyrrhizae, Rhizoma Zingiberis, the Radix Paeoniae Alba, Radix Et Rhizoma Rhei etc. and Radix Aconiti Lateralis Preparata compatibility in the classic prescriptions such as " Medical Treasures of the Golden Chamber " and treatise on Febrile Diseases, mainly play the effect of detoxifcation: namely absorb with the body that reduces poisonous alkaloids by reducing diester-type alkaloids content or changing metabolic pathway.Concocting also is the main method of Radix Aconiti Lateralis Preparata attenuation, sends Wu's weighing apparatus of wearing to wait doctor's domestic Radix Aconiti Lateralis Preparata to follow 3 principles as the god of fire: the one, and usefulness process of preparing Chinese medicine Radix Aconiti Lateralis Preparata; The 2nd, use with compatibilities such as Rhizoma Zingiberis, last Cortex Cinnamomis; The 3rd, fry in shallow oil for a long time.Wherein fry in shallow oil the most exquisitely for a long time, heavy dose of often fried in shallow oil 3 hours down and taste it, treat after half an hour not numb mouthful, just with its medicine with decoction it.Yet above-mentioned method of attenuating still can't guarantee the safety of Radix Aconiti Lateralis Preparata clinical practice, does not develop real nontoxic Radix Aconiti Lateralis Preparata.Trace it to its cause or the clinical unified standard that the degree of association boundary of Radix Aconiti Lateralis Preparata toxicity, dosage, concocting method is failed to understand and lacked the process of preparing Chinese medicine of Radix Aconiti Lateralis Preparata attenuation.In addition, invigorating YANG school uses heavy dose of Radix Aconiti Lateralis Preparata not build consensus clinically, disagrees with state-promulgated pharmacopoeia, has restricted giving full play to of Radix Aconiti Lateralis Preparata drug effect.The application and development achievement that the present invention obtains at the problems referred to above research just has application promise in clinical practice.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists, and a kind of preparation technology that can remove the Radix Aconiti Lateralis Preparata acute toxicity fully and keep its drug activity is provided.This class poisonous alkaloids of this technology utilization easily is heated the principle of hydrolysis, sets up Radix Aconiti Lateralis Preparata detoxification new technology.The chmice acute toxicity test shows do not possess acute toxicity fully by the Radix Aconiti Lateralis Preparata decocting liquid behind this technology detoxification; Toxic component aconitine in the efficient liquid phase chromatographic analysis proof Radix Aconiti Lateralis Preparata is degraded into compositions such as aconine fully in preparation process; The effect experiment result discloses, and the Radix Aconiti Lateralis Preparata behind the detoxification still possesses anti-inflammatory activity fully, can be to rat assist agent arthritis model performance drug action.
The objective of the invention is to finish by following technical solution.This preparation technology that can remove the Radix Aconiti Lateralis Preparata acute toxicity fully and keep its drug activity, this technology comprises following step: 1) get medium sized mud Radix Aconiti Lateralis Preparata, dry after cleaning and weigh, add the edible gallbladder Ba Shui of 25L and 12.5L clear water in every 50kg Radix Aconiti Lateralis Preparata and soaked 7 days; 2) decocting in water was put in the clear water behind the extremely saturating heart and was soaked 24 hours in 1 hour, and de-epithelization soaked 10 hours again; 3) rip cutting is 2 ~ 3mm thin slice, places clear water and soaks 10 hours, dries to the roll set angle, shines or dries by the fire to absolutely dry after smoking with sulfur; 4) add to soak after 30 minutes in 5 ~ 10 times of amount volume water and boil, uncoveredly fry in shallow oil 2 hours (intense fire boils, slow fire boiling 2 hours) for a long time; 5) obtain nontoxic Radix Aconiti Lateralis Preparata medicinal liquid behind the centrifuging and taking supernatant, concentrate the back and use.The detoxification Radix Aconiti Lateralis Preparata medicinal liquid that obtains, efficient liquid phase chromatographic analysis show that it does not contain the aconitine composition, contain mesaconitine (0.56 ± 0.02 μ g/g) and hypaconitine (8.73 ± 0.13 μ g/g) on a small quantity; Chmice acute toxicity detects proof, and it does not have an acute toxicity, and the highest filling stomach dosage (190g/kg) is mice survival rate 100% down; The rat pharmacological experiment discloses it and has anti-inflammatory activity, can effectively improve the adjuvant arthritis rats symptom.
As preferably, after step 4), with four layers of filtered through gauze, time decocting liquid of winning, medicinal residues add 4 ~ 8 times of amount volume water again, and intense fire boils back slow fire boiling 2 hours, with four layers of filtered through gauze, gets decocting liquid for the second time, discards medicinal residues; Merge decocting liquid twice, decocting liquid is condensed into extractum (50 ° of C concentrate on Rotary Evaporators), be the described Radix Aconiti Lateralis Preparata extract of removing acute toxicity fully and keeping drug activity.The Radix Aconiti Lateralis Preparata extract of half an hour, 1 hour is fried in shallow oil in preparation simultaneously for a long time, carries out following compositions, toxicity and drug effect relatively with detoxification Radix Aconiti Lateralis Preparata in the invention.
The aconite alkaloids composition be main effective ingredient in the Radix Aconiti Lateralis Preparata also be hypertoxic composition, mainly contain aconitine (aconitine), mesaconitine (mesaconitine) and hypaconitine (hypaconitine), can produce tangible Cardiovascular Toxicity, neurotoxicity and gastrointestinal toxicity.Diterpene diester-type alkaloids composition hydrolysis that can this class is poisonous by processing procedures such as decoctions becomes nontoxic diterpene non-ester-type alkaloid.Accordingly, the present invention uses the HPLC method content of frying in shallow oil half an hour, 1 hour and the contained above-mentioned three kinds of main toxic components of detoxification Radix Aconiti Lateralis Preparata is for a long time measured, when estimating detoxification technology, disclosing detoxification mechanism, for this technology provides the reference of respective quality control criterion.This is first technical parameter provided by the invention.Further, the present invention determines to fry in shallow oil for a long time 2 hours for best process conditions by the chmice acute toxicity test, is better than frying in shallow oil for a long time half an hour and 1 hour, can remove the Radix Aconiti Lateralis Preparata acute toxicity fully.This is another technical parameter provided by the invention, and also the quality control standard for this technology provides reference.
In addition, the present invention is by making up the rat assist agent arthritis model, to frying in shallow oil half an hour for a long time, 1 hour, and the detoxification Radix Aconiti Lateralis Preparata is low, in, the antiinflammatory drug effect of detoxification Radix Aconiti Lateralis Preparata is estimated under the high dose, still can improve adjuvant-induced arthritis behind the complete detoxification of index testing result proof Radix Aconiti Lateralis Preparata characterizes, with the half an hour that does not reach the detoxification effect, it is identical to fry in shallow oil the Radix Aconiti Lateralis Preparata effect in 1 hour for a long time, can be by suppressing the expression performance antiinflammatory action of il-1 (IL-1) and tumor necrosis factor (TNF-α), thereby the detoxification Radix Aconiti Lateralis Preparata among proof the present invention still can keep drug activity, has using value preferably.
Beneficial effect of the present invention is mainly reflected in: set up Radix Aconiti Lateralis Preparata detoxification technology and correlated quality control criterion thereof first; Prove the drug action of nontoxic Radix Aconiti Lateralis Preparata first and be applied to the experimentation of rheumatoid arthritis, for Radix Aconiti Lateralis Preparata clinical practice safely and effectively and diagnosis and treatment scheme optimization provide scientific basis.Method is easy, stable, and cost is low, can guarantee the complete detoxification of Radix Aconiti Lateralis Preparata, and for Radix Aconiti Lateralis Preparata provides safeguard in clinical Secure Application, expection can produce good economic and social benefit.
Description of drawings
Fig. 1 is the hydrolytic process sketch map of three kinds of poisonous diterpene diester-type alkaloids.
Fig. 2 is the high-efficient liquid phase chromatogram of the contained three kinds of poisonous alkaloids of Radix Aconiti Lateralis Preparata under the different detoxification conditions; Raw Fuzi: Radix Aconiti Lateralis; Bfp: the Radix Aconiti Lateralis Preparata of the common process of preparing Chinese medicine; DBfp-30: fry in shallow oil 30 minutes detoxification Radix Aconiti Lateralis Preparata for a long time; DBfp-60: fry in shallow oil 60 minutes detoxification Radix Aconiti Lateralis Preparata for a long time; DBfp-120: fry in shallow oil 120 minutes detoxification Radix Aconiti Lateralis Preparata for a long time; Wherein fry in shallow oil after 120 minutes Radix Aconiti Lateralis Preparata aconitine composition for a long time by complete hydrolysis.
Fig. 3 is that the content of the contained three kinds of poisonous alkaloids of Radix Aconiti Lateralis Preparata under the different detoxification conditions compares sketch map;
Fig. 4 causes cumulative mortality and the LD of mice for three kinds of detoxification Radix Aconiti Lateralis Preparatas (dBfp-30, dBfp-60, dBfp-120) in the acute toxicity testing
50Compare sketch map;
Fig. 5 be in the pharmacological evaluation three kinds of detoxification Radix Aconiti Lateralis Preparatas (dBfp-30, dBfp-60, dBfp-120) to the improvement effect sketch map of adjuvant arthritis rats pedal swelling degree;
Fig. 6 be in the pharmacological evaluation three kinds of detoxification Radix Aconiti Lateralis Preparatas (dBfp-30, dBfp-60, dBfp-120) based on the antiinflammatory action sketch map of interleukin and tumor necrosis factor.
The specific embodiment
Below by the present invention is further elaborated with the specific embodiment by reference to the accompanying drawings, embodiment will help to understand the present invention better, but the present invention is not limited only to following embodiment.
Reagent and material:
Radix Aconiti Lateralis (river, Sichuan oil), Radix Aconiti Lateralis Preparata (decoction pieces factory of Zhejiang University of Traditional Chinese Medicine, lot number: 100424), more than all through being accredited as the daughter root processed goods of ranunculaceae plant Aconitum carmichjaelii Debx. (Aconitum carmichaeli Debx).Aconitine aconitine(Nat'l Pharmaceutical ﹠ Biological Products Control Institute, lot number: 0720-9406), mesaconitine mesaconitine(Nat'l Pharmaceutical ﹠ Biological Products Control Institute, lot number: 0799-9203), hypaconitine hypaconitine(Nat'l Pharmaceutical ﹠ Biological Products Control Institute, lot number: 0798-9202); Acetonitrile (U.S. TEDIA reagent company), oxolane are chromatographically pure; Isopropyl alcohol, ethyl acetate, dichloromethane, ammonia, ammonium acetate, acetic acid etc. are analytical pure.Incomplete Freund (Freund's Incomplete Adjuvant): specification: 10ml/ props up, and SIGMA company produces, lot number: 908024LC.Freund's complete adjuvant FCA preparation: bacillus calmette-guerin vaccine added to be made among the FIA contain the FCA that bacillus calmette-guerin vaccine concentration is 10mg/ml, in agitator, vibrate, fully emulsified.
Laboratory animal and raising condition: the Kunming mice of SPF level body weight 180 ~ 220g male Wistar rat and body weight 18 ~ 22g male and female half and half provides production licence number by Shanghai Experimental Animal Center/Shanghai Slac Experimental Animal Co., Ltd. of the Chinese Academy of Sciences: SCXK(Shanghai) 2007-0005.Raise in barrier environment, 22 ± 1 ℃ of temperature, relative humidity 50 ~ 70% changes the wind number of times 15 ~ 20 times/hour, illumination 150 ~ 200Lx, light and shade replaced in 12 hours, and noise＜50dB is provided with control and display system automatically such as temperature, humidity, illumination, barometric gradient; The Co60 irradiation full nutrition pellet of feeding, occupancy permit: SYXK(Zhejiang) 2008-0115.
High performance liquid chromatograph (waters e2695, online degasser, quaternary pump, automatic sampler, column oven, VWD detector); 100,000/electronic balance (BS224S, German Sai Duolisi), ultrasonic machine, Chinese medicine grinder etc.UV-detector (German Knauer company, K-2501); Electronic balance (German Sai Duolisi, BS224S); L-210 type eddy mixer (the next prosperous science and trade in Beijing Co., Ltd) electric furnace, stainless-steel pan etc.
Embodiment 1: the preparation of detoxification Radix Aconiti Lateralis Preparata extract
Get medium sized mud Radix Aconiti Lateralis Preparata, dry after cleaning and weigh, add the edible gallbladder Ba Shui of 25L and 12.5L clear water in every 50kg Radix Aconiti Lateralis Preparata and soaked 7 days, decocting in water 1 hour is put in the clear water to the saturating heart and was soaked 24 hours, and de-epithelization soaks that rip cutting is 2 ~ 3mm thin slice after 10 hours again, placing clear water soaked 10 hours, dry to the roll set angle, shine again or dry by the fire to absolutely dry after smoking with sulfur, add 5～10 times of water gagings, soak more than the 30min, uncovered, intense fire boils, slow fire boiling 2 hours.With four layers of filtered through gauze, time decocting liquid of winning.Medicinal residues add 4 ~ 8 times of water gagings again, and intense fire boils back slow fire boiling 2 hours, with four layers of filtered through gauze, get decocting liquid for the second time, discard medicinal residues.Merge decocting liquid twice, gained detoxification Radix Aconiti Lateralis Preparata decocting liquid is condensed into extractum (50 ° of C concentrate on Rotary Evaporators), carry out dissolved dilution by variable concentrations before using.
Embodiment 2: chromatography and the assay of the contained poisonous aconitine of detoxification Radix Aconiti Lateralis Preparata
The preparation of reference substance solution: precision is measured mesaconitine reference substance 2.5981mg, aconitine reference substance 3.3345mg, hypaconitine reference substance 6.8230mg, and standardize solution is made three single mark reference substance solution in the 5mL volumetric flask respectively.Get an amount of 1mL, 0.5mL, 1mL respectively from above each reference substance, standardize solution is prepared into the hybrid standard product in the 10mL volumetric flask.
The preparation of need testing solution: remove malicious Radix Aconiti Lateralis Preparata extractum 10g, the accurate title, add ammonia solution 5mL, whirlpool 2min in the fixation tool plug conical flask.The accurate ether 50mL that adds blows and beats 1min repeatedly with pasteur pipet, and standing over night is divided and got ether layer, and lower floor uses the ether washed twice again, and each 25mL blows and beats 1min repeatedly with pasteur pipet, leaves standstill 30min, divides and gets ether layer.Merge three times ether layer, after low temperature reclaims, use the hydrochloric acid methanol standardize solution in the 5mL volumetric flask.
Chromatographic condition: chromatographic column HypersiLBDSC18; Detect wavelength 235nm; Flow velocity 1.0minmL-1; 30 ℃ of column temperatures; Mobile phase is acetonitrile-0.1% triethylamine (45:55).
Methodological study: precision is investigated: accurate absorption mesaconitine, hypaconitine, aconitine to the 5mL volumetric flask, are analyzed under above-mentioned chromatographic condition in right amount, and continuous sample introduction 5 times calculates peak area and RSD; Study on the stability: precision takes by weighing 1 part of detoxification Radix Aconiti Lateralis Preparata extractum, is prepared into need testing solution, under above-mentioned chromatographic condition, analyze, respectively 0,4,8,12,24, the 48h sample introduction, calculate peak area RSD; Linear relationship is investigated: the above-mentioned reference substance mixed solution of accurate absorption, and adopt a times times dilution process to get series standard solution, the accurate 20 μ L that draw analyze under identical chromatographic conditions, are abscissa with the sample concentration, and peak area is that vertical coordinate carries out linear regression; Repeatability is investigated: precision takes by weighing 6 parts of detoxification Radix Aconiti Lateralis Preparata extractum, is prepared into need testing solution, analyzes under identical chromatographic conditions, calculates content and RSD; Average recovery is investigated: precision takes by weighing the detoxification Radix Aconiti Lateralis Preparata extractum of 9 parts of known content, and not commensurability mesaconitine, aconitine and the hypaconitine reference substance of accurate adding according to being prepared into need testing solution analysis, calculates average recovery and RSD respectively.
The assay of poisonous diterpene diester-type alkaloids in the detoxification Radix Aconiti Lateralis Preparata: the method for learning in investigating prepares need testing solution according to the method described above, the accurate 20 μ L of absorption inject chromatograph of liquid, under above-mentioned chromatographic condition, analyze, three parts of every duplicate samples parallel assays calculate content and RSD according to external standard method.
The result shows: detoxification Radix Aconiti Lateralis Preparata mesaconitine is by complete hydrolysis, and concentration content is 0; Mesaconitine and hypaconitine content significantly reduce.
Embodiment 3: detoxification Radix Aconiti Lateralis Preparata acute toxicity is estimated
Fasting 12h after the above-mentioned Kunming mice group irritates stomach again and gives various dose (70,100,130,160,190g/kg) detoxification Radix Aconiti Lateralis Preparata.The blank group gives the equal volume sterilized water.Observed 14 days continuously, during carry out general physiology and detect and mortality statistics, and calculate median lethal dose(LD 50) LD
50, maximum tolerated dose MTD, minimal lethal dose MLD, no observed adverse effect level NOAEL etc.
The result shows: the detoxification Radix Aconiti Lateralis Preparata does not show acute toxicity to mice, and fatality rate is 0.
Embodiment 4: detoxification Radix Aconiti Lateralis Preparata antiinflammatory drug effect evaluation of effect
Above-mentioned Wistar rat is carried out random packet, go out normal group and carry out the adjuvant-induced arthritis modeling outward: the right back sufficient pawl intradermal injection of rat contains the Freund's complete adjuvant 0.1ml of bacillus calmette-guerin vaccine 10mgml-1.Every animal 1ml/100g irritates stomach and gives detoxification Radix Aconiti Lateralis Preparata (14g/kg crude drug amount), continuous 14 days.Viewing duration adopts quantitative or semiquantitative method, and body weight, anus temperature, dietary amount, amount of drinking water, urine amount, the wet degree of big dry stool, depilation, the excitation respond of each group rat are observed, detected.Carried out once in per 3 days.The rat paw edema degree is measured: measured rat toes volume in per 3 days with sufficient sole of the foot volumetric type meter, the test position is right back sufficient sole of the foot portion (to ankle joint), (0 day) test basic value before the modeling, it namely is the pedal swelling variable quantity that later each test gained result deducts basic value.
Pedal swelling rate E%=(Vt-Vn)/Vn
Annotate: Vn causes scorching preceding volume V t and causes scorching back volume.
The preparation of serum sample: with 10% chloral hydrate solution, 3ml/kg, anesthetized rat, abdominal vein is got blood and is put in the centrifuge tube, the centrifugal 10min(4 of 3000rpm ℃), separation of serum is managed packing with eppendorf, according to IL-1 and TNF-α test kit description operation, on the microplate reader of wavelength 450nm, detect its OD value.Being vertical coordinate with the OD value, is abscissa curve plotting figure with standard substance concentration, and OD value is per sample tried to achieve the concentration of each sample.Measurement data is relatively checked with t in twos; Checking its diversity, is the standard of its significance of difference with P＜0.05 or P＜0.01.All data all adopt SPSS17 statistical package statistics.
The result shows: the detoxification Radix Aconiti Lateralis Preparata can change the foot swelling degree (P＜0.01) of obviously kind adjuvant arthritis rats.And by regulating serum il-1, TNF-alpha levels (P＜0.01) performance antiinflammatory action.
At last, should be pointed out that above example only is the more representational example of the present invention.Obviously, technical scheme of the present invention is not limited to above-mentioned example, and many distortion can also be arranged, and all distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (2)
1. preparation technology that can remove the Radix Aconiti Lateralis Preparata acute toxicity fully and keep its drug activity, it is characterized in that: this technology comprises following step: 1) get medium sized mud Radix Aconiti Lateralis Preparata, dry after cleaning and weigh, add the edible gallbladder Ba Shui of 25L and 12.5L clear water in every 50kg Radix Aconiti Lateralis Preparata and soaked 7 days; 2) decocting in water was put in the clear water behind the extremely saturating heart and was soaked 24 hours in 1 hour, and de-epithelization soaked 10 hours again; 3) rip cutting is 2 ~ 3mm thin slice, places clear water and soaks 10 hours, dries to the roll set angle, shines or dries by the fire to absolutely dry after smoking with sulfur; 4) immersion was boiled uncovered frying in shallow oil for a long time 2 hours after 30 minutes in 5 ~ 10 times of amounts of adding volume water; 5) obtain nontoxic Radix Aconiti Lateralis Preparata medicinal liquid behind the centrifuging and taking supernatant, concentrate the back and use.
2. the preparation technology that can remove the Radix Aconiti Lateralis Preparata acute toxicity fully and keep its drug activity according to claim 1, it is characterized in that: after step 4), with four layers of filtered through gauze, win time decocting liquid, medicinal residues add 4 ~ 8 times of amount volume water again, and intense fire boils back slow fire boiling 2 hours, with four layers of filtered through gauze, get decocting liquid for the second time, discard medicinal residues; Merge decocting liquid twice, decocting liquid is condensed into extractum, be Radix Aconiti Lateralis Preparata extract.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113069487A (en) * | 2021-03-31 | 2021-07-06 | 建昌帮药业有限公司 | Radix aconiti lateralis praeparata tablet and preparation method thereof |
| CN116942735A (en) * | 2023-06-15 | 2023-10-27 | 浙江中医药大学 | Fuzi decoction granule capable of removing toxicity and retaining drug effect and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113069487A (en) * | 2021-03-31 | 2021-07-06 | 建昌帮药业有限公司 | Radix aconiti lateralis praeparata tablet and preparation method thereof |
| CN113069487B (en) * | 2021-03-31 | 2022-05-20 | 建昌帮药业有限公司 | Radix aconiti lateralis praeparata tablet and preparation method thereof |
| CN116942735A (en) * | 2023-06-15 | 2023-10-27 | 浙江中医药大学 | Fuzi decoction granule capable of removing toxicity and retaining drug effect and preparation method thereof |
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