CN103173391A - Method for applying bacteria on culture medium panel through balls - Google Patents
Method for applying bacteria on culture medium panel through balls Download PDFInfo
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- CN103173391A CN103173391A CN2013100877606A CN201310087760A CN103173391A CN 103173391 A CN103173391 A CN 103173391A CN 2013100877606 A CN2013100877606 A CN 2013100877606A CN 201310087760 A CN201310087760 A CN 201310087760A CN 103173391 A CN103173391 A CN 103173391A
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Abstract
The invention discloses a method for applying bacteria on a culture medium panel through balls. The method comprises the steps of: heating, sterilizing, drying and cooling balls to the room temperature for standby application; dripping a bacterium suspension liquid onto a culture medium panel, then adding the balls, covering a petri dish cover of the culture medium panel, and shaking the culture medium panel until the balls uniformly apply the bacterium suspension liquid on the surface of the culture medium panel; and opening the petri dish cover to pour the balls out, and sterilizing and cleaning the balls for repeated use. According to the method, the balls serve as application tools, the culture medium panel is shaken, and the balls are adhered with the bacterium suspension liquid to freely roll on the surface of the culture medium panel, so that the bacterium suspension liquid is uniformly applied. A rolling application mode is adopted, the surface of the culture medium panel is not easy to scratch, the bacterium suspension liquid is uniformly applied, the application efficiency is high and quick; most importantly, ball application is utilized, opening or semi-opening operation does not need, the contamination degree is decreased; and moreover, the balls can be sterilized and stored in a centralized manner, and the time for preparing the application tools is shortened.
Description
Technical field
The invention belongs to the Experiment on Microbiology technical field, be specifically related to a kind of quick, efficient is high, pollute the method for few culture medium flat plate rolling ball type coating bacterium.
Background technology
The coating bacterium is method commonly used in Experiment on Microbiology on culture medium flat plate, is widely used in the operations such as bacterium separation, bacterial count, drug sensitive test, antagonism detection.Utilize the culture medium flat plate culturing bacterium usually to adopt the spreading rod hardened coating, although easy, but this mode easily scratches media surface, more difficult coating is even, the problems such as personal errors also easily appear, and spreading rod operational requirement flat board must be in and open wide or half opening-wide state, and substratum and air are long duration of contact, are easy to cause living contaminants.For this reason, develop a kind of quick, efficient is high, pollute few coating bacterium method, is very favorable for Experiment on Microbiology.
Summary of the invention
The object of the present invention is to provide a kind of quick, efficient is high, pollute the method for few culture medium flat plate rolling ball type coating bacterium.
The object of the present invention is achieved like this, comprises the following steps:
A, ball sterilization: with dry after the ball heat sterilization, be cooled to room temperature standby;
B, the coating of rolling: bacterial suspension is dripped on culture medium flat plate, add ball, build the culture dish lid of culture medium flat plate, the wave and culture base is dull and stereotyped, until ball evenly is coated with the culture medium flat plate surface with bacterial suspension; Open the culture dish lid, pour out ball, the reserve recycling is cleaned in sterilization.
As spreading implement, dull and stereotyped by the wave and culture base, ball adheres to bacterial suspension at the media surface Free-rolling with ball in the present invention, thereby realizes the even coating of bacterial suspension.Spreading rod of the prior art is the mode of coating of sliding, and the present invention has adopted the mode of the coating of rolling, and compares more difficult scuffing media surface with the coating of sliding, and is coated with more evenly, and efficient is higher, faster; Of paramount importance is to utilize the ball coating, needn't open wide or half unlimited operation, has reduced pollution level; Moreover ball can concentrate sterilization to deposit, and has shortened the time that the coating apparatus is prepared.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, but never in any form the present invention is limited, and any replacement that is equal to based on technical thought of the present invention all falls into protection scope of the present invention.
The method of culture medium flat plate rolling ball type coating bacterium of the present invention comprises the following steps:
A, ball sterilization: with dry after the ball heat sterilization, be cooled to room temperature standby;
B, the coating of rolling: bacterial suspension is dripped on culture medium flat plate, add ball, build the culture dish lid of culture medium flat plate, the wave and culture base is dull and stereotyped, until ball evenly is coated with the culture medium flat plate surface with bacterial suspension; Open the culture dish lid, pour out ball, the reserve recycling is cleaned in sterilization.
The described ball heat sterilization of A step is that ball is contained in heat sterilization in thermally resistant container.The described heat sterilization of A step is wet-heating or dry heating method sterilization.
Add 5 ~ 8 balls on the described culture medium flat plate of B step.Be further, the described culture medium flat plate of B step uses plastics or glass culture dish, and the diameter of culture dish is 60mm, 90mm, 100mm or 120mm, and ball is the smooth spherosome of diameter 3 ~ 8mm.Described ball is stainless steel, pottery or glass ball.
Dull and stereotyped manual mode or the mechanical system of adopting of the described wave and culture base of B step shaken.Described mechanical system is shaken and is shaken for universal stage or shaking table (having another name called vibrator).Other can be horizontal the device that rotates of selecting type all can realize purpose of the present invention.
Principle of work of the present invention:
As spreading implement, dull and stereotyped by the wave and culture base, ball adheres to bacterial suspension at the media surface Free-rolling with ball in the present invention, thereby realizes the even coating of bacterial suspension.Spreading rod of the prior art is the coating of sliding, and the present invention has adopted the mode of the coating of rolling, and compares more difficult scuffing media surface with the coating of sliding, and is coated with more evenly, and efficient is higher, faster; Of paramount importance is to utilize the ball coating, needn't open wide or half unlimited operation, has reduced pollution level; Moreover ball can concentrate sterilization to deposit, and has shortened the time that the coating apparatus is prepared.
Embodiment 1
Select 3mm stainless steel ball, ball is contained in thermally resistant container, after adopting the wet-heating sterilization, drying is cooled to room temperature standby; Select the 60mm plastic culture dish to prepare culture medium flat plate, bacterial suspension is dripped on culture medium flat plate, then add 5 balls, build dull and stereotyped culture dish lid, manual swing plate is until ball is with the even spread plate of bacterial suspension surface; Open the culture dish lid, pour out ball, standby after sterilization, cleaning.
Embodiment 2
Select the 8mm ceramic balls, ball is contained in thermally resistant container, after adopting the dry heating method sterilization, drying is cooled to room temperature standby; Select the 90mm glass culture dish to prepare culture medium flat plate, bacterial suspension is dripped on culture medium flat plate, then add 8 balls, build dull and stereotyped culture dish lid, use the universal stage swing plate, until ball is with the even spread plate of bacterial suspension surface; Open the culture dish lid, pour out ball, standby after sterilization, cleaning.
Embodiment 3
Select 5mm glass ball, ball is contained in thermally resistant container, after adopting the wet-heating sterilization, drying is cooled to room temperature standby; Select the 100mm plastic culture dish to prepare culture medium flat plate, bacterial suspension is dripped on culture medium flat plate, then add 7 balls, build dull and stereotyped culture dish lid, manual swing plate is until ball is with the even spread plate of bacterial suspension surface; Open the culture dish lid, pour out ball, standby after sterilization, cleaning.
Embodiment 4
Select 6mm glass ball, ball is contained in thermally resistant container, after adopting the wet-heating sterilization, drying is cooled to room temperature standby; Select the 120mm glass culture dish to prepare culture medium flat plate, bacterial suspension is dripped on culture medium flat plate, then add 6 balls, build dull and stereotyped culture dish lid, use the shaking table swing plate, until ball is with the even spread plate of bacterial suspension surface; Open the culture dish lid, pour out ball, standby after sterilization, cleaning.
Claims (8)
1. the method for culture medium flat plate rolling ball type coating bacterium is characterized in that comprising the following steps:
A, ball sterilization: with dry after the ball heat sterilization, be cooled to room temperature standby;
B, the coating of rolling: bacterial suspension is dripped on culture medium flat plate, add ball, build the culture dish lid of culture medium flat plate, the wave and culture base is dull and stereotyped, until ball evenly is coated with the culture medium flat plate surface with bacterial suspension; Open the culture dish lid, pour out ball, the reserve recycling is cleaned in sterilization.
2. culture medium flat plate rolling ball type according to claim 1 is coated with the method for bacterium, and it is characterized in that: the described ball heat sterilization of A step is that ball is contained in heat sterilization in thermally resistant container.
3. culture medium flat plate rolling ball type according to claim 1 and 2 is coated with the method for bacterium, it is characterized in that: the described heat sterilization of A step is wet-heating or dry heating method sterilization.
4. the method for culture medium flat plate rolling ball type coating bacterium according to claim 1, is characterized in that: add 5 ~ 8 balls on the described culture medium flat plate of B step.
5. according to claim 1 or 4 described culture medium flat plate rolling ball types are coated with the method for bacteriums, it is characterized in that: the described culture medium flat plate of B step uses plastics or glass culture dish, the diameter of culture dish is 60mm, 90mm, 100mm or 120mm, and ball is the smooth spherosome of diameter 3 ~ 8mm.
6. according to claim 1,2 or 4 described culture medium flat plate rolling ball types are coated with the method for bacteriums, and it is characterized in that: described ball is stainless steel, pottery or glass ball.
7. culture medium flat plate rolling ball type according to claim 1 is coated with the method for bacterium, it is characterized in that: dull and stereotyped manual mode or the mechanical system of adopting of the described wave and culture base of B step shaken.
8. the method for culture medium flat plate rolling ball type according to claim 7 coating bacterium is characterized in that: described mechanical system is shaken and is shaken for universal stage or shaking table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100877606A CN103173391A (en) | 2013-03-19 | 2013-03-19 | Method for applying bacteria on culture medium panel through balls |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100877606A CN103173391A (en) | 2013-03-19 | 2013-03-19 | Method for applying bacteria on culture medium panel through balls |
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| CN103173391A true CN103173391A (en) | 2013-06-26 |
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| CN2013100877606A Pending CN103173391A (en) | 2013-03-19 | 2013-03-19 | Method for applying bacteria on culture medium panel through balls |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024108395A1 (en) * | 2022-11-22 | 2024-05-30 | 深圳先进技术研究院 | Microbial liquid coating device and coating method therefor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440393A (en) * | 2008-12-15 | 2009-05-27 | 浙江大学 | Method for cultivating intestinal lactobacillus |
| CN101440395A (en) * | 2008-12-15 | 2009-05-27 | 浙江大学 | Intestinal bacteria culture method and culture medium thereof |
-
2013
- 2013-03-19 CN CN2013100877606A patent/CN103173391A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440393A (en) * | 2008-12-15 | 2009-05-27 | 浙江大学 | Method for cultivating intestinal lactobacillus |
| CN101440395A (en) * | 2008-12-15 | 2009-05-27 | 浙江大学 | Intestinal bacteria culture method and culture medium thereof |
Non-Patent Citations (1)
| Title |
|---|
| 卢汀 等: "用毛笔作柑橘绿霉平板涂布的技术改进", 《微生物学免疫学进展》, vol. 35, no. 4, 31 December 2007 (2007-12-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024108395A1 (en) * | 2022-11-22 | 2024-05-30 | 深圳先进技术研究院 | Microbial liquid coating device and coating method therefor |
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Application publication date: 20130626 |