CN103170007B - A kind of Biodegradable high-molecular porous urethra recovery support and preparation method - Google Patents
A kind of Biodegradable high-molecular porous urethra recovery support and preparation method Download PDFInfo
- Publication number
- CN103170007B CN103170007B CN201110436651.1A CN201110436651A CN103170007B CN 103170007 B CN103170007 B CN 103170007B CN 201110436651 A CN201110436651 A CN 201110436651A CN 103170007 B CN103170007 B CN 103170007B
- Authority
- CN
- China
- Prior art keywords
- urethra
- support
- recovery support
- recovery
- molecular porous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of Biodegradable high-molecular porous urethra recovery support, it is characterized in that, the tissue engineering bracket of described urethra rack to be a kind of face shaping be tubulose, by the Polymer materialspreparation that biology can be degradable, support softness has toughness, and support microstructure is cavernous three dimensions, and mean pore size is 20-200 μm, there is good biocompatibility, can repopulating cell.The present invention also discloses this urethra recovery support and obtains preparation method.Prepared urethra recovery support shows certain rigidity and toughness necessarily, can bear and there is powerful expansionary force, contribute to the recovery of support shape when extruding, and have good biocompatibility, mucous membrane of urethra epithelial cell can adhere to and breed on support.Prepared urethra recovery support avoids the residual of organic solvent and porogen, and biological assessment is safer, and preparation technology is easier, and cost is cheaper, is more suitable for clinical practice.
Description
Technical field
The present invention relates to a kind of urethra recovery support and preparation method, particularly relate to a kind of Biodegradable high-molecular porous urethra recovery support and preparation method, belong to medical science reparation and field of tissue engineering technology.
Background technology
Many congenital (as rugged shape) and posteriority factor (as wound, war wound, infection, tumor etc.) all can cause Urethral defect, narrow and dysfunction.Owing to lacking desirable repair materials, its reconstruction is the challenging difficult problem that Urology Surgery faces always.
For the treatment that suffer from the disease relevant with posterior urethral stricture of above-mentioned Urethral defect, normal with skin (mucosa) free grafting or partial-band distally-based flap Shift recovery clinically, but skin (mucosa) free grafting often causes urethral stricture because of contracture, it is also higher to urinate repeatly incidence rate; It is too fat to move that the transfer of partial-band distally-based flap then often causes local, and form is not good, and patient and family members are difficult to accept.Moreover, autograft, to sacrifice the Therapeutic mode of normal structure for cost " with operation wound repair tissue defect ", had both added new operation wound, had turn increased the probability of postoperative complication, and autologous tissue's limited source, limit its clinical practice.
So increasing medical tissue engineering material is used to urethra reconstruction, but various types of materials respectively has advantage and the defect of its uniqueness.
The earliest for the material of urethra reconstruction be a series of nondegradable synthetic material such as silica gel, ferrum cloth-wrapper dragon for urethra reconstruction, but occurred local erosion, displacement, carbuncle road, narrow, ooze out, the problem such as calcification.
In recent years the Biodegradable material of synthetic has been attempted again, as polycaprolactone (PCL), lactic acid-caprol acton copolymer (PLA-CL), lactic acid-ethanol copolymer (PLGA), polylactic acid (PLA) etc., cell experiment and zoopery all obtain certain effect, but there is no clinical practice data.
Meanwhile, the also favourable report spending cellular matrix reconstruction urethra, zoopery and clinical practice demonstrate certain advantage, but this type of material source is very limited, and being used for repairing urethra long section tubulose defect obviously has unrealistic property.
Total score is above-mentioned three kinds of materials, generally believes and has Biodegradable material only, and the three-dimensional porous rack particularly prepared by Biodegradable high-molecular likely finds a kind of urethra recovery support of reasonably artificial preparation to replace autotransplantation.
Summary of the invention
The object of the invention is to provide one can final biodegradable high molecular urethra rack and preparation method thereof, for the needs that injury of urethra is repaired.
The invention provides a kind of Biodegradable high-molecular porous urethra recovery support, it is characterized in that, the tissue engineering bracket of described urethra rack to be a kind of face shaping be tubulose, by the Polymer materialspreparation that biology can be degradable, support softness has toughness, and support microstructure is cavernous three dimensions, and mean pore size is 20-200 μm, there is good biocompatibility, can repopulating cell.
Described biology can be degradable macromolecular material be polycaprolactone (PCL), lactic acid-caprol acton copolymer (PLA-CL), polylactic acid (PLA), a kind of or its combination in hydroxybutyric acid-hydroxycaproic acid copolymer (PHBHHx), one component is necessary for polycaprolactone, or lactic acid-caprol acton copolymer.
Described lactic acid-caprol acton copolymer, the mol ratio of its lactic acid and caprolactone is 7: 3 ~ 9: 1.
The invention provides a kind of preparation method of Biodegradable high-molecular porous urethra recovery support, it is characterized in that comprising the steps:
(1) macromolecular material that biology can be degradable is dissolved in organic solvent completely, forms oil phase;
(2) ammonium bicarbonate solubility is in water, dissolves completely under room temperature, forms aqueous phase;
(3) aqueous phase and oil phase mixed and carry out homogenized, forming the emulsion of thickness;
(4) promptly successively the mould of special diameter is inserted in emulsion, extract out and normal temperature drying after stirring at once, then sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
In described oil phase, organic solvent is one in chloroform, dichloromethane, ethyl acetate or its combination.
In described oil phase, the proportion of macromolecular material is 10%-50% (g/100ml).
In described aqueous phase, the solution concentration of ammonium bicarbonate is 10-20% (g/100ml).
The volume ratio of described aqueous phase and oil phase is 1: 2 ~ 1: 4.
Urethra recovery support prepared by the present invention has good biocompatibility, promotes the propagation of cell; This urethra recovery support does not have the residual of other impurity will and organic solvent; This urethra recovery support inside is three-D space structure, the pore size of support can by the difference of materials and process regulable control, for adhesion and the propagation of cell seed.The internal diameter of urethra recovery support can by the diameter control of mould.Above-mentioned mould can be smooth Glass rod, stainless steel bar, prioritizing selection Glass rod.The preparation technology of above-mentioned urethra recovery support is comparatively simple, the lower cost of preparation.
The finished product of biodegradable high molecular urethra recovery support disclosed by the invention does not have the residual of porogen and organic solvent, biological assessment is safer, preparation technology is easier, and cost is cheaper, is more suitable for the application of the clinical repair of field of tissue engineering technology and urethra.Prepared urethra recovery support shows certain rigidity and toughness necessarily, can bear and there is powerful expansionary force, contribute to the recovery of support shape when extruding, and have good biocompatibility, mucous membrane of urethra epithelial cell can adhere to and breed on support.Prepared urethra recovery support avoids the residual of organic solvent and porogen, and biological assessment is safer, and preparation technology is easier, and cost is cheaper, is more suitable for clinical practice.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of urethra recovery support prepared by the present invention.
1 is urethra recovery support internal diameter, and 2 is the loose structure of urethra recovery support.
Fig. 2 is biodegradable high molecular urethra rack pictorial diagram.
Fig. 3 is the scanning electron microscope diagram of the microstructure of biodegradable high molecular urethra rack.
Detailed description of the invention
Embodiment 1:
Prepare the polycaprolactone urethra recovery support that interior diameter is respectively 2.5mm, 3.5mm, 4.5mm.
Taking 4gPCL is dissolved in the dichloromethane of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 3 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, but extruding after-poppet can not be returned to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
By sem observation, find that the urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.By the size in scanning electron microscope special-purpose software random measurement 1000 holes, and average and can obtain the mean pore size of interior three-dimensional space structure, in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 2:
Prepare PLA-CL (7: 3) the urethra recovery support that interior diameter is respectively 2.5mm, 3.5mm, 4.5mm.
Taking 4gPLA-CL (7: 3) is dissolved in the dichloromethane/chloroform (volume shared by dichloromethane is 80%) of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data are in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 3:
Prepare PLA-CL (8: 2) the urethra recovery support that interior diameter is 2.5mm, 3.5mm, 4.5mm.
Taking 4gPLA-CL (8: 2) is dissolved in the dichloromethane/ethyl acetate (volume shared by dichloromethane is 70%) of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 4 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data are in table 2.By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 4:
Prepare PLA-CL (9: 1) the urethra recovery support that interior diameter is 2.5mm, 3.5mm, 4.5mm.
Taking 4gPLA-CL (9: 1) is dissolved in the dichloromethane of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The mean pore size of interior three-dimensional space structure is in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 5:
Prepare the urethra recovery support that interior diameter is respectively PLA-CL (7: the 3)/PLGA composite of 2.5mm, 3.5mm, 4.5mm.
The PLGA taking 2gPLA-CL (7: 3) and 2g is dissolved in the dichloromethane of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 3 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data are in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 6:
Prepare the urethra recovery support that interior diameter is respectively PLA-CL (7: the 3)/PHBHHx composite of 2.5mm, 3.5mm, 4.5mm.
The PHBHHx taking 2gPLA-CL (7: 3) and 2g is dissolved in the dichloromethane of 20ml, and dissolving in 1 hour is dissolved in heating also condensing reflux, forms oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase be the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, forming the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data are in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
Embodiment 7:
Prepare the urethra recovery support that interior diameter is respectively PLA-CL (7: the 3)/PLGA/PHBHHx composite of 2.5mm, 3.5mm, 4.5mm.
Take 2gPLA-CL (7: 3), the PHBHHx of PLGA and 1g of 1g is dissolved in the dichloromethane of 20ml, heating condensing reflux dissolves dissolvings in 1 hour, formation oil phase.Take 15g ammonium bicarbonate solubility in 100ml water, normal-temperature dissolution, to dissolving completely, forms aqueous phase.By aqueous phase and oil phase by volume 1: 2 ratio mixing and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.Be promptly in the Glass rod insertion emulsion of 2.5mm, 3.5mm, 4.5mm successively by diameter, extract out after stirring and normal temperature drying 12 hours at once, again sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretches, curling or doubling all can not cause fracture, extruding after-poppet can deformation recovery to original diameter and size.
The urethra recovery support of high-molecular porous shape soaks front and back and has certain change in phosphate buffer solution, and its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter is in table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can for the attachment of cell and growth.The mean pore size of interior three-dimensional space structure is in table 2.
By 10
6the individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts 37 DEG C, cultivates in the cell culture incubator of 5.0% carbon dioxide.Cultivate 1 day successively, 4 days, 7 days, and carry out the proliferative conditions of cell on urethra recovery support by cytoactive test kit (CCK-8).Wherein, prepare interior diameter and be respectively the related data of the urethra recovery support propagation of 3.5mm in table 3.
The urethra recovery support of the high-molecular porous shape of table 1. soaks the mean size (mm) of front and back internal diameter in phosphate buffer solution
The mean pore size (μm) of the urethra recovery support interior three-dimensional space structure of the high-molecular porous shape of table 2.
Table 3. internal diameter is that the urethra recovery support of 3.5mm is respectively in the cytoactive test kit testing result 1 day, 4 days and 7 days
Note: all data are the meansigma methods of the light absorption value under 450nm wavelength, n=4.
Claims (1)
1. the preparation method of a Biodegradable high-molecular porous urethra recovery support, the tissue engineering bracket of described urethra rack to be a kind of face shaping be tubulose, by the Polymer materialspreparation that biology can be degradable, support softness has toughness, support microstructure is cavernous three dimensions, mean pore size is 20 – 200 μm, has good biocompatibility, can repopulating cell; Described biology can be degradable macromolecular material be polycaprolactone (PCL), lactic acid-caprol acton copolymer (PLA-CL), polylactic acid (PLA), a kind of or its combination in hydroxybutyric acid-hydroxycaproic acid copolymer (PHBHHx), one component is necessary for polycaprolactone, or lactic acid-caprol acton copolymer; Described lactic acid-caprol acton copolymer, the mol ratio of its lactic acid and caprolactone is 7:3 ~ 9:1; It is characterized in that comprising the steps:
(1) macromolecular material that biology can be degradable is dissolved in organic solvent completely, forms oil phase;
(2) ammonium bicarbonate solubility is in water, dissolves completely under room temperature, forms aqueous phase;
(3) aqueous phase and oil phase mixed and carry out homogenized, forming the emulsion of thickness;
(4) promptly successively the mould of special diameter is inserted in emulsion, extract out and normal temperature drying after stirring at once, then sample is carried out lyophilization process removing organic solvent, namely obtain the urethra recovery support of high-molecular porous shape;
In described oil phase, organic solvent is one in chloroform, dichloromethane, ethyl acetate or its combination;
In described oil phase, the proportion of macromolecular material is 10%-50% g/100ml;
In described aqueous phase, the solution concentration of ammonium bicarbonate is 10-20% g/100ml;
The volume ratio of described aqueous phase and oil phase is 1:2 ~ 1:4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110436651.1A CN103170007B (en) | 2011-12-22 | 2011-12-22 | A kind of Biodegradable high-molecular porous urethra recovery support and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110436651.1A CN103170007B (en) | 2011-12-22 | 2011-12-22 | A kind of Biodegradable high-molecular porous urethra recovery support and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103170007A CN103170007A (en) | 2013-06-26 |
| CN103170007B true CN103170007B (en) | 2015-09-09 |
Family
ID=48630481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110436651.1A Expired - Fee Related CN103170007B (en) | 2011-12-22 | 2011-12-22 | A kind of Biodegradable high-molecular porous urethra recovery support and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103170007B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITMI20131716A1 (en) * | 2013-10-16 | 2015-04-17 | Antonio Sambusseti | ABSORBABLE TUBULAR TUBULAR BRACKET FOR TUBULAR PATCH INTENDED FOR TISSUE RECONSTRUCTION OF URETRAL AND / OR URETERAL SECTORS REMOVED |
| CN109847101B (en) * | 2018-12-28 | 2022-04-12 | 广州市妇女儿童医疗中心 | Tissue engineering urethral stent and preparation process thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513900A (en) * | 2003-07-30 | 2004-07-21 | 复旦大学 | Degradable tubular polymeric multipore foaming material and its preparation method |
| CN1973828A (en) * | 2006-11-10 | 2007-06-06 | 中国人民解放军第二军医大学 | VEGF slowly releasing injection microsphere support and its prepn and use |
| CN101891881A (en) * | 2009-05-21 | 2010-11-24 | 中国科学院化学研究所 | Biodegradable high-polymer additive, preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001078687A1 (en) * | 2000-04-18 | 2001-10-25 | Peptron Inc. | Injectable sustained release pharmaceutical composition and processes for preparing the same |
-
2011
- 2011-12-22 CN CN201110436651.1A patent/CN103170007B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513900A (en) * | 2003-07-30 | 2004-07-21 | 复旦大学 | Degradable tubular polymeric multipore foaming material and its preparation method |
| CN1973828A (en) * | 2006-11-10 | 2007-06-06 | 中国人民解放军第二军医大学 | VEGF slowly releasing injection microsphere support and its prepn and use |
| CN101891881A (en) * | 2009-05-21 | 2010-11-24 | 中国科学院化学研究所 | Biodegradable high-polymer additive, preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 可注射聚丙交酯多孔微载体制备及与骨髓基质干细胞共培养研究;王暾等;《组织工程与重建外科杂志》;20060831;第2卷(第4期);第190页左栏1.2 微载体的制备部分、第191页右栏第2段 * |
| 微载体培养技术的研究与进展;周燕等;《中国组织工程研究与临床康复》;20100416;第14卷(第16期);2945-2948 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103170007A (en) | 2013-06-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kankala et al. | Highly porous microcarriers for minimally invasive in situ skeletal muscle cell delivery | |
| ES2408554T3 (en) | Method for preparing porous framework for tissue engineering, cell culture and cell supply | |
| Wang et al. | Enhancing the bioactivity of Poly (lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model | |
| Zhu et al. | Kombucha‐synthesized bacterial cellulose: Preparation, characterization, and biocompatibility evaluation | |
| Xie et al. | Evaluation of stretched electrospun silk fibroin matrices seeded with urothelial cells for urethra reconstruction | |
| Dai et al. | Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) as an injectable implant system for prevention of post-surgical tissue adhesion | |
| Hu et al. | Biodegradable poly (lactic acid-co-trimethylene carbonate)/chitosan microsphere scaffold with shape-memory effect for bone tissue engineering | |
| Sadiasa et al. | In vitro and in vivo evaluation of porous PCL-PLLA 3D polymer scaffolds fabricated via salt leaching method for bone tissue engineering applications | |
| CN104353110B (en) | For the bone holder material with shape memory function and preparation method thereof of jawbone reparation | |
| Ardeshirylajimi et al. | Biomimetic scaffold containing PVDF nanofibers with sustained TGF-β release in combination with AT-MSCs for bladder tissue engineering | |
| Liu et al. | Surface modification of porous PLGA scaffolds with plasma for preventing dimensional shrinkage and promoting scaffold–cell/tissue interactions | |
| Agostino et al. | Semi-interpenetrated hydrogels composed of PVA and hyaluronan or chondroitin sulphate: chemico-physical and biological characterization | |
| CN107670108A (en) | A kind of tissue engineering bracket polylactic acid porous material and preparation method thereof | |
| CN109876186A (en) | A kind of biological medical degradable double-layer scaffold and preparation method thereof for neural restoration | |
| Park et al. | Synthesis of elastic biodegradable polyesters of ethylene glycol and butylene glycol from sebacic acid | |
| Xu et al. | Biodegradable scaffolds for urethra tissue engineering based on 3D printing | |
| CN103656757A (en) | Double-layer intravascular stent carrying heparin and preparation method thereof | |
| CN102604149B (en) | Three-dimensional chitosan hydrogel and preparation method thereof | |
| Yang et al. | Double-modified bacterial cellulose/soy protein isolate composites by laser hole forming and selective oxidation used for urethral repair | |
| Revati et al. | Biodegradable poly (lactic acid) scaffold for tissue engineering: A brief review | |
| CN103170007B (en) | A kind of Biodegradable high-molecular porous urethra recovery support and preparation method | |
| CN104448356A (en) | Blank PLGA microspheres and preparation method thereof | |
| Zhang et al. | Electrospun coaxial nanofibers loading with perovskite and icariin to enhance the bone scaffold-mediated osteogenesis | |
| CN103654999B (en) | Nerve rehabilitating tube support of multiple structure and preparation method thereof | |
| Chang et al. | In vivo degradation of poly (ε-caprolactone) films in Gastro Intestinal (GI) tract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150909 Termination date: 20171222 |