CN103170007A - Biodegradable macromolecule urethra repairing support and preparation method thereof - Google Patents
Biodegradable macromolecule urethra repairing support and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a biodegradable macromolecule urethra repairing support. The biodegradable macromolecule urethra repairing support is characterized by being a tissue engineering support with a tubular appearance shape and being prepared from macromolecular materials which can be completely biodegraded, the support is soft and has toughness, the microstructure of the support is a porous three-dimensional space, the average pore size is 20-200 microns, the support has good biocompatibility, and cells can be planted. The invention also discloses a preparation method of the biodegradable macromolecule urethra repairing support. The prepared urethra repairing support shows certain rigidity and necessary toughness, can endure strong tensile force, and is conducive to restoration of the shape of the support when being extruded and has good biocompatibility, and urethra mucous epithelium cells can be adhered and propagated on the support. According to the prepared urethra repairing support, residual of organic solvents and pore-foaming agents sare avoided, biological assessment is safer, a preparation process is simpler and more convenient, cost is lower, and the prepared urethra repairing support is more applicable to clinical application.
Description
Technical field
The present invention relates to a kind of urethra recovery support and preparation method, particularly relate to a kind of Biodegradable high-molecular porous urethra recovery support and preparation method, belong to medical science reparation and field of tissue engineering technology.
Background technology
Many congenital (as rugged shape) and posteriority factor (as wound, war wound, infection, tumor etc.) all can cause Urethral defect, narrow and dysfunction.Owing to lacking desirable repair materials, its reconstruction is the challenging difficult problem that Urology Surgery faces always.
Treatment for above-mentioned Urethral defect take a disease disease relevant with posterior urethral stricture, often shift with skin (mucosa) free grafting or partial-band distally-based flap clinically and repair, but skin (mucosa) free grafting often causes urethral stricture because of contracture, urinates repeatly incidence rate also higher; The transfer of partial-band distally-based flap often causes local too fat to move, and form is not good, and patient and family members are difficult to accept.Moreover, autograft had both increased new operation wound to sacrifice normal structure as the treatment pattern of cost " damaged with the operation wound repair tissue ", had increased again the probability of postoperative complication, and the autologous tissue source is limited, has limited its clinical practice.
So increasing medical tissue engineering material is used to the urethra reconstruction, but various types of materials respectively has its unique advantage and defective.
The material that is used for the earliest the urethra reconstruction is that a series of nondegradable synthetic materials such as silica gel, ferrum cloth-wrapper dragon are used for the urethra reconstruction, but occurred local erosion, displacement, carbuncle road, narrow, ooze out, the problem such as calcification.
In recent years attempted again the Biodegradable material of synthetic, as polycaprolactone (PCL), lactic acid-caprolactone copolymer (PLA-CL), lactic acid-ethanol copolymer (PLGA), polylactic acid (PLA) etc., cell experiment and zoopery all obtain certain effect, but there is no the clinical practice data.
Simultaneously, also favourablely spend the report that cellular matrix is rebuild urethra, zoopery and clinical practice demonstrate certain advantage, but the materials source is very limited, are used for repairing that the long section of urethra tubulose is damaged that unrealistic property obviously arranged.
Total points is above-mentioned three kinds of materials, generally believes to have Biodegradable material only, and particularly the prepared three-dimensional porous rack of Biodegradable high-molecular might find a kind of urethra recovery support of reasonably artificial preparation to replace autotransplantation.
Summary of the invention
The object of the invention is to provide a kind of finally biodegradable high molecular urethra rack and preparation method thereof, is used for the needs that injury of urethra is repaired.
The invention provides a kind of Biodegradable high-molecular porous urethra recovery support, it is characterized in that, described urethra rack is that a kind of face shaping is the tissue engineering bracket of tubulose, by biology can be degradable Polymer materialspreparation, the support softness has toughness, and the support microstructure is cavernous three dimensions, and mean pore size is 20-200 μ m, have good biocompatibility, but repopulating cell.
Described biology can be degradable macromolecular material be a kind of or its combination in polycaprolactone (PCL), lactic acid-caprolactone copolymer (PLA-CL), polylactic acid (PLA), hydroxybutyric acid-hydroxycaproic acid copolymer (PHBHHx), the one component is necessary for polycaprolactone, or lactic acid-caprolactone copolymer.
Described lactic acid-caprolactone copolymer, the mol ratio of its lactic acid and caprolactone are 7: 3～9: 1.
The invention provides a kind of preparation method of Biodegradable high-molecular porous urethra recovery support, it is characterized in that comprising the steps:
(1) macromolecular material that biology can be degradable is dissolved in organic solvent fully, forms oil phase;
(2) ammonium bicarbonate is dissolved in the water, and under room temperature, dissolving fully, form water;
(3) with water with oil phase mixes and carry out homogenized, form the emulsion of thickness;
(4) promptly successively the mould of special diameter is inserted in emulsion, extract out and normal temperature drying after stirring at once, then sample is carried out lyophilization process and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
In described oil phase, organic solvent is a kind of or its combination in chloroform, dichloromethane, ethyl acetate.
In described oil phase, the proportion of macromolecular material is 10%-50% (g/100ml).
The solution concentration of described aqueous phase ammonium bicarbonate is 10-20% (g/100ml).
The volume ratio of described water and oil phase is 1: 2～1: 4.
The urethra recovery support of the present invention's preparation has good biocompatibility, promotes the propagation of cell; This urethra recovery support does not have the residual of other impurity will and organic solvent; This urethra recovery support inside is three-D space structure, and the pore size of support can be regulated control by the difference of material and technique, is used for adhesion and the propagation of cell seed.The internal diameter of urethra recovery support can be by the diameter control of mould.Above-mentioned mould can be smooth Glass rod, stainless steel bar is preferentially selected Glass rod.The preparation technology of above-mentioned urethra recovery support is simpler, the lower cost of preparation.
The finished product of biodegradable high molecular urethra recovery support disclosed by the invention does not have the residual of porogen and organic solvent, biological assessment is safer, preparation technology is easier, and cost is cheaper, more is applicable to the application of the clinical repair of field of tissue engineering technology and urethra.Prepared urethra recovery support shows certain rigidity and toughness necessarily, can bear and have powerful expansionary force, help the recovery of support shape in the time of extruding, and have good biocompatibility, the mucous membrane of urethra epithelial cell can adhere on support and breed.Prepared urethra recovery support has been avoided the residual of organic solvent and porogen, and biological assessment is safer, and preparation technology is easier, and cost is cheaper, more is applicable to clinical practice.
Description of drawings:
Fig. 1 is the structural representation of the urethra recovery support for preparing of the present invention.
1 is urethra recovery support internal diameter, and 2 is the loose structure of urethra recovery support.
Fig. 2 is biodegradable high molecular urethra rack pictorial diagram.
Fig. 3 is the scanning electron microscope diagram of the microstructure of biodegradable high molecular urethra rack.
The specific embodiment
Embodiment 1:
The preparation interior diameter is respectively the polycaprolactone urethra recovery support of 2.5mm, 3.5mm, 4.5mm.
Take in the dichloromethane that 4gPCL is dissolved in 20ml, heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 3 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, but the extruding after-poppet can not be returned to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
By sem observation, find that the urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.By the size in 1000 holes of scanning electron microscope special-purpose software random measurement, and average and to obtain the mean pore size of interior three-dimensional space structure, see Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 2:
The preparation interior diameter is respectively PLA-CL (7: 3) the urethra recovery support of 2.5mm, 3.5mm, 4.5mm.
Take in dichloromethane/chloroform (the shared volume of dichloromethane is 80%) that 4gPLA-CL (7: 3) is dissolved in 20ml, heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data see Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 3:
The preparation interior diameter is PLA-CL (8: 2) the urethra recovery support of 2.5mm, 3.5mm, 4.5mm.
Take in the dichloromethane/ethyl acetate (the shared volume of dichloromethane is 70%) that 4gPLA-CL (8: 2) is dissolved in 20ml, heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 4 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data see Table 2.With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 4:
The preparation interior diameter is PLA-CL (9: 1) the urethra recovery support of 2.5mm, 3.5mm, 4.5mm.
Take 4gPLA-CL (9: 1) and be dissolved in the dichloromethane of 20ml, heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The mean pore size of interior three-dimensional space structure sees Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 5:
The preparation interior diameter is respectively the urethra recovery support of the PLA-CL (7: 3) of 2.5mm, 3.5mm, 4.5mm/PLGA composite.
The PLGA that takes 2gPLA-CL (7: 3) and 2g is dissolved in the dichloromethane of 20ml, and heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 3 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data see Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 6:
The preparation interior diameter is respectively the urethra recovery support of the PLA-CL (7: 3) of 2.5mm, 3.5mm, 4.5mm/PHBHHx composite.
The PHBHHx that takes 2gPLA-CL (7: 3) and 2g is dissolved in the dichloromethane of 20ml, and heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and oil phase are the ratio mixing of 1: 2 by volume and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The measuring method of the mean pore size of interior three-dimensional space structure is with embodiment 1, and data see Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
Embodiment 7:
The preparation interior diameter is respectively the urethra recovery support of the PLA-CL (7: 3) of 2.5mm, 3.5mm, 4.5mm/PLGA/PHBHHx composite.
Take the PLGA of 2gPLA-CL (7: 3), 1g and the PHBHHx of 1g and be dissolved in the dichloromethane of 20ml, heating and condensing reflux dissolving dissolving in 1 hour form oil phase.Take the 15g ammonium bicarbonate and be dissolved in 100ml water, normal-temperature dissolution forms water to dissolving fully.Water and the oil phase ratio of 1: 2 are by volume mixed and carry out homogenized with the speed of 2500 revolution per seconds, form the emulsion of thickness.The Glass rod that with diameter is successively promptly 2.5mm, 3.5mm, 4.5mm inserts in emulsion, after stirring, extraction at once and normal temperature drying are 12 hours, again sample is carried out the lyophilization processing and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
The urethra recovery support of the high-molecular porous shape of gained has good toughness, moderately stretch, curling or doubling all can not cause fracture, the extruding after-poppet can deformation recovery to original diameter and size.
Have certain variation before and after the urethra recovery support of high-molecular porous shape soaks in phosphate buffer solution, its internal diameter has contraction to a certain degree, and before and after soaking, the size of internal diameter sees Table 1.
The urethra recovery support inside of high-molecular porous shape has good three-D space structure, can adhering to and growing for cell.The mean pore size of interior three-dimensional space structure sees Table 2.
With 10
6The individual eugonic rat mucous membrane of urethra epithelial cell that goes down to posterity is inoculated on prepared urethra recovery support, puts in 37 ℃, the cell culture incubator of 5.0% carbon dioxide and cultivates.Cultivated successively 1 day, 4 days, 7 days, and carry out the propagation situation of cell on the urethra recovery support by cytoactive test kit (CCK-8).Wherein, the preparation interior diameter related data that is respectively the urethra recovery support propagation of 3.5mm sees Table 3.
The mean size (mm) of internal diameter before and after the urethra recovery support of the high-molecular porous shape of table 1. soaks in phosphate buffer solution
The mean pore size (μ m) of the urethra recovery support interior three-dimensional space structure of the high-molecular porous shape of table 2.
Table 3. internal diameter is that the urethra recovery support of 3.5mm is respectively in the cytoactive test kit testing result 1 day, 4 days and 7 days
Annotate: all data are the meansigma methods of the light absorption value under the 450nm wavelength, n=4.
Claims (8)
1. Biodegradable high-molecular porous urethra recovery support, it is characterized in that, described urethra rack is that a kind of face shaping is the tissue engineering bracket of tubulose, by biology can be degradable Polymer materialspreparation, the support softness has toughness, the support microstructure is cavernous three dimensions, and mean pore size is 20-200 μ m.
2. a kind of Biodegradable high-molecular porous urethra recovery support according to claim 1, it is characterized in that, described biology can be degradable macromolecular material be a kind of or its combination in polycaprolactone (PCL), lactic acid-caprolactone copolymer (PLA-CL), polylactic acid (PLA), hydroxybutyric acid-hydroxycaproic acid copolymer (PHBHHx), the one component is necessary for polycaprolactone, or lactic acid-caprolactone copolymer.
3. a kind of Biodegradable high-molecular porous urethra recovery support according to claim 1, is characterized in that, described lactic acid-caprolactone copolymer, and the mol ratio of its lactic acid and caprolactone is 7: 3～9: 1.
4. according to claim 1, or 2, or the preparation method of 3 described a kind of Biodegradable high-molecular porous urethra recovery supports, it is characterized in that comprising the steps:
(1) macromolecular material that biology can be degradable is dissolved in organic solvent fully, forms oil phase;
(2) ammonium bicarbonate is dissolved in the water, and under room temperature, dissolving fully, form water;
(3) with water with oil phase mixes and carry out homogenized, form the emulsion of thickness;
(4) promptly successively the mould of special diameter is inserted in emulsion, extract out and normal temperature drying after stirring at once, then sample is carried out lyophilization process and remove organic solvent, namely obtain the urethra recovery support of high-molecular porous shape.
5. a kind of preparation method of Biodegradable high-molecular porous urethra recovery support according to claim 4, is characterized in that, in described oil phase, organic solvent is a kind of or its combination in chloroform, dichloromethane, ethyl acetate.
6. a kind of preparation method of Biodegradable high-molecular porous urethra recovery support according to claim 4, is characterized in that, in described oil phase, the proportion of macromolecular material is 10%-50% (g/100ml).
7. a kind of preparation method of Biodegradable high-molecular porous urethra recovery support according to claim 4, is characterized in that, the solution concentration of described aqueous phase ammonium bicarbonate is 10-20% (g/100ml).
8. a kind of preparation method of Biodegradable high-molecular porous urethra recovery support according to claim 4, is characterized in that, the volume ratio of described water and oil phase is 1: 2～1: 4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105792776A (en) * | 2013-10-16 | 2016-07-20 | 安东尼奥·桑布塞蒂 | PGA tubular patch and relative optional tubular support made of absorbable material for tissue reconstruction of urethral and/or ureteral removed segments |
| CN109847101A (en) * | 2018-12-28 | 2019-06-07 | 广州市妇女儿童医疗中心 | A kind of Tissue Engineering Urethra bracket and its preparation process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030026844A1 (en) * | 2000-04-18 | 2003-02-06 | Hee-Yong Lee | Injectable sustained release pharmaceutical composition and processes for preparing the same |
| CN1513900A (en) * | 2003-07-30 | 2004-07-21 | 复旦大学 | Degradable tubular polymeric multipore foaming material and its preparation method |
| CN1973828A (en) * | 2006-11-10 | 2007-06-06 | 中国人民解放军第二军医大学 | VEGF slowly releasing injection microsphere support and its prepn and use |
| CN101891881A (en) * | 2009-05-21 | 2010-11-24 | 中国科学院化学研究所 | Biodegradable high-polymer additive, preparation method and application thereof |
-
2011
- 2011-12-22 CN CN201110436651.1A patent/CN103170007B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030026844A1 (en) * | 2000-04-18 | 2003-02-06 | Hee-Yong Lee | Injectable sustained release pharmaceutical composition and processes for preparing the same |
| CN1513900A (en) * | 2003-07-30 | 2004-07-21 | 复旦大学 | Degradable tubular polymeric multipore foaming material and its preparation method |
| CN1973828A (en) * | 2006-11-10 | 2007-06-06 | 中国人民解放军第二军医大学 | VEGF slowly releasing injection microsphere support and its prepn and use |
| CN101891881A (en) * | 2009-05-21 | 2010-11-24 | 中国科学院化学研究所 | Biodegradable high-polymer additive, preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 周燕等: "微载体培养技术的研究与进展", 《中国组织工程研究与临床康复》 * |
| 王暾等: "可注射聚丙交酯多孔微载体制备及与骨髓基质干细胞共培养研究", 《组织工程与重建外科杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105792776A (en) * | 2013-10-16 | 2016-07-20 | 安东尼奥·桑布塞蒂 | PGA tubular patch and relative optional tubular support made of absorbable material for tissue reconstruction of urethral and/or ureteral removed segments |
| CN109847101A (en) * | 2018-12-28 | 2019-06-07 | 广州市妇女儿童医疗中心 | A kind of Tissue Engineering Urethra bracket and its preparation process |
| CN109847101B (en) * | 2018-12-28 | 2022-04-12 | 广州市妇女儿童医疗中心 | Tissue engineering urethral stent and preparation process thereof |
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