CN103169033A - Extraction process of strawberry phenol substances - Google Patents
Extraction process of strawberry phenol substances Download PDFInfo
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- CN103169033A CN103169033A CN2013101319857A CN201310131985A CN103169033A CN 103169033 A CN103169033 A CN 103169033A CN 2013101319857 A CN2013101319857 A CN 2013101319857A CN 201310131985 A CN201310131985 A CN 201310131985A CN 103169033 A CN103169033 A CN 103169033A
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- strawberry
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Abstract
The invention relates to an extraction process of strawberry phenol substances, which belongs to the field of food processing. The extraction process comprises the following steps of: randomly picking undamaged strawberries with the basically same ripeness; removing stalks and leaves of the picked strawberries, and homogenizing the strawberries in a high-speed tissue stamping machine; weighing a right amount of strawberry slurry and adding an ethanol solution with the concentration of 70% to the slurry in a solid-liquid ratio of 1:10; oscillating the mixture at a constant temperature of 40 DEG C and leaching the mixture for 40min; after the extraction, centrifuging the mixture at a speed of 3500rpm for 15min; filtering supernatant by filtering paper; then filling the filtered supernatant into a brown bottle to be preserved at -4 DEG C for further use; detecting the total phenol content of the solution in a Folin-Ciocalteu colorimetric method; and drying an extracted solution at a low temperature to obtain the phenol substances of the strawberries. The extraction process of the strawberry phenol substances disclosed by the invention is economical and practical as well as simple and feasible, is good for obtaining the needed strawberry phenol substances, and provides certain reference evidences for further researching and developing strawberry phenol active products and functional foods with market prospect.
Description
One, technical field
The present invention is a kind of extraction process of strawberry aldehydes matter, belongs to food processing field.
Two, background technology
Strawberry (Fragaria ananassa Duch) is one of the world's seven large health fruits.China's strawberry production development has in recent years become world strawberry the first big producing country rapidly at present, no matter is that cultivated area or output all occupy first place in the world.2012, the strawberry area of China reached 80,000 hectares, and annual production has reached 1,880,000 tons.Yet the development of China Strawberry industry is but very slow, and product is mainly domestic marketing fresh and exported frozen, a small amount of strawberry fruit juice, fruit wine etc. of being processed as, based on the intensive processing product of active material seldom.
Strawberry contains mineral matter and the trace element of abundant vitamin, amino acid, polysaccharide, cellulose, needed by human, and strawberry is rich in the phenolic active components such as phenolic acid, anthocyanidin, Flavonoid substances in addition.Phenolic compound is the general name of Polyhydroxy phenol, has physiologically active widely, as stronger antioxidation, and obvious antibacterial, anticancer, treatment cardiovascular and cerebrovascular disease, anti-aging and suppress the effect such as cholesterol rising, absorb the generation that a certain amount of plant phenolics could prevent and suppress disease effectively.The extraction of aldehydes matter is the first step of its utilization.At present the extracting method of plant polyphenol mainly contains: solvent extraction, Microwave-assisted Extraction are got, ultrasonic wave assisted extraction, biological enzymolysis extraction, ion precipitation extraction, supercritical fluid extraction, membrane technology extraction etc.Solvent extraction is one of present domestic most popular extracting method.Solvent extraction method mainly contains that aqueous solvent extracts and two kinds of organic solvent extractions, due to simple process, cost is low, purity is high is widely used.It is to utilize the different solubility of polyphenol in different solvents to carry out refluxing extraction that organic solvent extracts, and solvent commonly used has methyl alcohol, ethanol, acetone, ethyl acetate etc., and this method can improve recovery rate, Reaction time shorten.Other organic solvent toxicities are lower relatively due to ethanol, and this process using ethanol is as extracting solvent.
In plant, polyphenol compound has physiologically active widely, plant polyphenol is utilized by commercial development at present, as vine polyphenol, pine bark polyphenol (pyenogenol, Pycnogenol), Tea Polyphenols, apple polyphenol etc., be widely used in the association areas such as medical science, food, process hides and daily-use chemical industry, and brought into play irreplaceable effect.Along with cultivated area and the output of China strawberry increases gradually, eat rear remaining a large amount of strawberries raw and be difficult to process.The extraction process of polyphenols in strawberry has the strawberry phenols biologically active prod of market prospects and functional food that certain reference frame is provided for further researching and developing.
Three, summary of the invention
Technical problem the present invention mainly is to provide a kind of extraction process of strawberry aldehydes matter.
Technical scheme the present invention mainly is to provide a kind of extraction process of strawberry aldehydes matter, has comprised following steps:
1) sampling: pluck strawberry at random from the orchard, select after impurity elimination without wound, maturity basically identical, extract immediately or-20 ℃ of quick-frozens standby.
2) homogenate: fresh strawberry or the strawberry after thawing are removed the handle leaf, put into high-speed tissue mashing machine's homogenate.
3) extract: take the strawberry slurries appropriate, add 70% ethanolic solution with the solid-liquid ratio amount of 1: 10,40 ℃ of constant temperature oscillations, lixiviate 40min.
4) filter: extract complete after the centrifugal 15min of 3500rpm, get supernatant and pack into after with Filter paper filtering in brown bottle-4 ℃ and save backup.
5) mensuration of total phenol content: adopt the Folin-Ciocalteu colorimetric method.The sample solution (0.2mL) of getting suitable dilution and filtration adds respectively 2mL0.5M Folin-Ciocalteu reagent, and then standing 4min at room temperature after mixing uses 2mL saturated sodium carbonate (75g/L) neutralization.Measure its light absorption value with spectrophotometer under the 760nm wavelength after in the dark placing 2h under room temperature condition.Measure under the same conditions the gallic acid light absorption value drawing standard curve with variable concentrations, result is expressed as gallic acid equivalant concentration, i.e. mg gallic acid/g dry weight.
6) drying: the extract low temperature drying gets total aldehydes matter of strawberry.
Beneficial effect the present invention is intended to set up a kind of technique of simple, high efficiency extraction strawberry aldehydes matter.The lower ethanol of this process selection toxicity is as extracting solvent, and concentration is 70%.From the aspect of energy saving economy, select 40 ℃ of suitable extraction temperature, Yin Gaowen can weaken or destroy the integrality of cell membrane, solvent is more contacted with aldehydes matter, improve recovery rate, but excess Temperature can destroy the aldehydes matter structure, reduces its biologically active.Higher solid-liquid ratio is conducive to the stripping of polyphenols, but the utilization rate of material and solvent is considered in the excessive waste that also can cause resource of solid-liquid ratio, finally determines that extracting solid-liquid ratio is 1: 10.Sufficient extraction time can make solvent fully enter strawberry histocyte each position, improves recovery rate, but long extraction time easily cause the oxidation of aldehydes matter, therefore selecting extraction time is 40min.The total phenol yield of this technique is high, good stability, is suitable for industrial production, can be further strawberry essence deep processed product exploitation and lays the first stone.
Four, the specific embodiment
The invention will be further described below in conjunction with specific embodiment:
Embodiment 1
Strawberry is adopted the Guo Chang in Nanjing Xindeli Food Co., Ltd., and kind is the strawberry " Feng Xiang " of introducing from Japan.All strawberries are plucked from the orchard at random, select without wound, maturity basically identically after impurity elimination, and the handle leaf is removed in immediately-20 ℃ of quick-frozens after thawing, and put into after high-speed tissue mashing machine's homogenate standby after weighing.Take strawberry slurries 2g, add 70% ethanolic solution 20mL, 40 ℃ of constant temperature water bath vibrations, lixiviate 30min, extract complete after the centrifugal 15min of 3500rpm, get supernatant and pack into after with Filter paper filtering in brown bottle-4 ℃ and save backup.Adopt Folin-Ciocalteu colorimetric method for determining total phenol content, triplicate extracts, and the yield of total phenol is all more than 0.8mg/g.
Claims (6)
1. the extraction process of a strawberry aldehydes matter has comprised following steps:
1) sampling: pluck strawberry at random from the orchard, select after impurity elimination without wound, maturity basically identical, extract immediately or-20 ℃ of quick-frozens standby.
2) homogenate: fresh strawberry or the strawberry after thawing are removed the handle leaf, put into high-speed tissue mashing machine's homogenate.
3) extract: take the strawberry slurries appropriate, add 70% ethanolic solution with the solid-liquid ratio amount of 1: 10,40 ℃ of constant temperature oscillations, lixiviate 40min.
4) filter: extract complete after the centrifugal 15min of 3500rpm, get supernatant and pack into after with Filter paper filtering in brown bottle-4 ℃ and save backup.
5) mensuration of total phenol content: adopt the Folin-Ciocalteu colorimetric method.The sample solution (0.2mL) of getting suitable dilution and filtration adds respectively 2mL0.5M Folin-Ciocalteu reagent, and then standing 4min at room temperature after mixing uses 2mL saturated sodium carbonate (75g/L) neutralization.Measure its light absorption value with spectrophotometer under the 760nm wavelength after in the dark placing 2h under room temperature condition.Measure under the same conditions the gallic acid light absorption value drawing standard curve with variable concentrations, result is expressed as gallic acid equivalant concentration, i.e. mg gallic acid/g dry weight.
6) drying: the extract low temperature drying gets total aldehydes matter of strawberry.
2. a kind of extraction process of strawberry aldehydes matter according to claim 1 is characterized in that: pluck at random strawberry, select without wound, maturity basically identical after impurity elimination.
3. a kind of extraction process of strawberry aldehydes matter according to claim 1, it is characterized in that: strawberry is removed the handle leaf, puts into high-speed tissue mashing machine's homogenate.
4. a kind of extraction process of strawberry aldehydes matter according to claim 1, it is characterized in that: extracting solid-liquid ratio is 1: 10, extracts solvent and be 70% ethanolic solution, and extracting temperature is 40 ℃, and extraction time is 40min.
5. a kind of extraction process of strawberry aldehydes matter according to claim 1, it is characterized in that: the mensuration of total phenol content is to adopt the Folin-Ciocalteu colorimetric method
6. a kind of extraction process of strawberry aldehydes matter according to claim 1, it is characterized in that: low temperature drying gets total aldehydes matter of strawberry.
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| CN2013101319857A CN103169033A (en) | 2013-04-17 | 2013-04-17 | Extraction process of strawberry phenol substances |
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| CN2013101319857A CN103169033A (en) | 2013-04-17 | 2013-04-17 | Extraction process of strawberry phenol substances |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304582A (en) * | 2021-12-28 | 2022-04-12 | 广州昆特臣香精香料科技有限公司 | Strawberry extract and preparation method thereof |
Citations (4)
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| CN101610688A (en) * | 2006-08-09 | 2009-12-23 | 科雷森斯有限公司 | Polyphenol extracting method |
| CN101648980A (en) * | 2009-08-28 | 2010-02-17 | 广东轻工职业技术学院 | Method for combined preparation of pineapple polyphenol and juice by taking pineapple peel as main material and application thereof |
| CN101658290A (en) * | 2009-09-21 | 2010-03-03 | 黑龙江八一农垦大学 | Method for extracting crabapple polyphenols from crabapple skin dregs |
| CN102924536A (en) * | 2012-11-06 | 2013-02-13 | 山东省农业科学院农产品研究所 | Preparation method of strawberry polyphenol |
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2013
- 2013-04-17 CN CN2013101319857A patent/CN103169033A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101610688A (en) * | 2006-08-09 | 2009-12-23 | 科雷森斯有限公司 | Polyphenol extracting method |
| CN101648980A (en) * | 2009-08-28 | 2010-02-17 | 广东轻工职业技术学院 | Method for combined preparation of pineapple polyphenol and juice by taking pineapple peel as main material and application thereof |
| CN101658290A (en) * | 2009-09-21 | 2010-03-03 | 黑龙江八一农垦大学 | Method for extracting crabapple polyphenols from crabapple skin dregs |
| CN102924536A (en) * | 2012-11-06 | 2013-02-13 | 山东省农业科学院农产品研究所 | Preparation method of strawberry polyphenol |
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| 刘文旭等: "草莓、黑莓、蓝莓中多酚类物质及其抗氧化活性研究", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304582A (en) * | 2021-12-28 | 2022-04-12 | 广州昆特臣香精香料科技有限公司 | Strawberry extract and preparation method thereof |
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Application publication date: 20130626 |