CN103163251A - Essence aroma composition analysis method combining GC/MS and aroma smelling instrument - Google Patents
Essence aroma composition analysis method combining GC/MS and aroma smelling instrument Download PDFInfo
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- CN103163251A CN103163251A CN 201310108245 CN201310108245A CN103163251A CN 103163251 A CN103163251 A CN 103163251A CN 201310108245 CN201310108245 CN 201310108245 CN 201310108245 A CN201310108245 A CN 201310108245A CN 103163251 A CN103163251 A CN 103163251A
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Abstract
The invention relates to an essence aroma composition analysis method combining GC/MS and an aroma smelling instrument. The essence aroma composition analysis method is characterized by comprising the steps of: 1) extraction of essence aroma; and 2) combination of GC/MS and the aroma smelling instrument: shunting aroma composition obtained in the step 1) after entering GC, wherein one branch of the aroma composition is subjected to MS identification, and the other branch of the aroma composition is smelled by a valuator, so that testing results are true and accurate. By combining the GC/MS and the aroma smelling instrument, the essence aroma composition analysis method overcomes the defects that the GC/MS can not be used for determining the contribution of various aroma compounds to various aroma, and the combination of instrumental analysis and sensory evaluation is very meaningful to the identification of characteristic aroma compounds and aroma active compounds, as well as the determination of the aroma intensity and the contribution of all the components, so that the essence aroma composition analysis method is a novel and comprehensive aroma analysis means.
Description
Technical field
The present invention relates to a kind of analytical approach of essence fragrance component, particularly a kind of essence analysis of aroma components method of GC/MS and the coupling of Wen Xiang instrument.
Background technology
In the process of the essence fragrance component being analyzed with GC/MS, there is following defective: can't determine that various volatile compounds are to the size of overall fragrance contribution, the inventive method adopts solid phase micro-extraction method (SPME), GC/MS is participated in the discussion of fragrant instrument coupling carry out the essence analysis of aroma components, determine the crucial fragrance component of essence with this, for the research and development of assay essence quality and novel essence provide theoretical foundation.
Summary of the invention
The objective of the invention is to overcome GC/MS in prior art and can't be used in essence analysis of aroma components process determining that various volatile compounds to the shortcoming of the size of overall fragrance contribution, provide a kind of essence analysis of aroma components method of GC/MS and the coupling of Wen Xiang instrument.
The technical solution used in the present invention is:
A kind of essence analysis of aroma components method of GC/MS and the coupling of Wen Xiang instrument comprises the steps:
1), the extraction of essence fragrance;
2), GC/MS participates in the discussion of fragrant instrument coupling; With step 1) after the aroma component that obtains enters GC, shunted, one is carried out MS and identifies, and another smells news by the valuation officer, makes testing result more true, accurately.
Preferably, described essence fragrance extraction step is: draw appropriate essence sample and appropriate amount of deionized water, stir evenly, put into the headspace sample bottle of 20mL, keep 30min on the constent temperature heater of 60 ℃, aging good solid phase micro-extraction extraction head is inserted carry out head space absorption, extraction time 30min in bottle.
Preferably, the condition of described GC (gas chromatography): HB-5 capillary column (30m * 0.25 μ m * 0.25 μ m), 250 ℃ of injector temperatures, carrier gas is high-purity (〉=99.999%) helium, and flow rate of carrier gas is 1.0mL/min, temperature programme: 40 ℃ of initial temperatures, keep 3.0min, 3 ℃/min rises to 80 ℃, keeps 1min, then rises to 230 ℃ of maintenance 10.0min with 15 ℃/min; Described MS(mass spectrum) condition: EI ion gun; Electron energy 70eV; 150 ℃ of level Four bar temperature, 260 ℃ of interface temperature; Electron-multiplier voltage 1654V; 230 ℃ of ion source temperatures; The employing standard mode is tuning; Select full scan (SCAN) pattern, mass scanning scope: 30～550amu.
The beneficial effect that the present invention has:
The present invention participates in the discussion of fragrant instrument coupling with GC/MS, solved GC/MS and can't be used for determining that various volatile compounds are to the shortcoming of the size of overall fragrance contribution, instrumental analysis is combined with sensory evaluation, to diagnostic characteristics volatile compound, fragrance reactive compound and be used for determining the aroma strength of each component and contribution highly significant, it is a kind of novel, comprehensive fragrance analysis means.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but do not limit protection scope of the present invention.
Embodiment 1
A kind of essence analysis of aroma components method of GC/MS and the coupling of Wen Xiang instrument comprises the steps:
1), the extraction of essence fragrance: draw 1g essence sample and 5mL deionized water, stir evenly, put into the headspace sample bottle of 20mL, keep 30min on the constent temperature heater of 60 ℃, aging good solid phase micro-extraction extraction head (commercially available prod) is inserted carry out head space absorption, extraction time 30min in bottle;
2), GC/MS participates in the discussion of fragrant instrument coupling; With step 1) after the aroma component that obtains enters GC, shunted, one is carried out MS and identifies, and another smells news by the valuation officer, makes testing result more true, accurately.
The condition of GC (gas chromatography): HB-5 capillary column (30m * 0.25 μ m * 0.25 μ m), 250 ℃ of injector temperatures, carrier gas is high-purity (〉=99.999%) helium, flow rate of carrier gas is 1.0mL/min, temperature programme: 40 ℃ of initial temperatures, keep 3.0min, 3 ℃/min rises to 80 ℃, keep 1min, then rise to 230 ℃ of maintenance 10.0min with 15 ℃/min;
The MS(mass spectrum) condition: EI ion gun; Electron energy 70eV; 150 ℃ of level Four bar temperature, 260 ℃ of interface temperature; Electron-multiplier voltage 1654V; 230 ℃ of ion source temperatures; The employing standard mode is tuning; Select full scan (SCAN) pattern, mass scanning scope: 30～550amu.
Claims (3)
1. the essence analysis of aroma components method of a GC/MS and the coupling of Wen Xiang instrument, is characterized in that: comprise the steps:
1), the extraction of essence fragrance.
2), GC/MS participates in the discussion of fragrant instrument coupling; With step 1) after the aroma component that obtains enters GC, shunted, one is carried out MS and identifies, and another smells news by the valuation officer.
2. a kind of essence analysis of aroma components method of GC/MS and the coupling of Wen Xiang instrument according to claim 1, it is characterized in that: described essence fragrance extraction step is: draw appropriate essence sample and appropriate amount of deionized water, stir evenly, put into the headspace sample bottle of 20mL, keep 30min on the constent temperature heater of 60 ℃, aging good solid phase micro-extraction extraction head is inserted carry out head space absorption, extraction time 30min in bottle.
3. the essence analysis of aroma components method of described a kind of GC/MS and the coupling of Wen Xiang instrument according to claim 1 and 2, it is characterized in that: the condition of described GC: HB-5 capillary column (30m * 0.25 μ m * 0.25 μ m), 250 ℃ of injector temperatures, carrier gas is high-purity (〉=99.999%) helium, and flow rate of carrier gas is 1.0mL/min, temperature programme: 40 ℃ of initial temperatures, keep 3.0min, 3 ℃/min rises to 80 ℃, keeps 1min, then rises to 230 ℃ of maintenance 10.0min with 15 ℃/min; The condition of described MS: EI ion gun; Electron energy 70eV; 150 ℃ of level Four bar temperature, 260 ℃ of interface temperature; Electron-multiplier voltage 1654V; 230 ℃ of ion source temperatures; The employing standard mode is tuning; Select full scan (SCAN) pattern, mass scanning scope: 30～550amu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201310108245 CN103163251A (en) | 2013-03-29 | 2013-03-29 | Essence aroma composition analysis method combining GC/MS and aroma smelling instrument |
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| CN 201310108245 CN103163251A (en) | 2013-03-29 | 2013-03-29 | Essence aroma composition analysis method combining GC/MS and aroma smelling instrument |
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| CN103163251A true CN103163251A (en) | 2013-06-19 |
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| CN 201310108245 Pending CN103163251A (en) | 2013-03-29 | 2013-03-29 | Essence aroma composition analysis method combining GC/MS and aroma smelling instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198805A (en) * | 2016-07-12 | 2016-12-07 | 中山大学惠州研究院 | A kind of detection method of spices and essence Controlled-Release Capability of Microcapsules |
| CN106645495A (en) * | 2016-12-29 | 2017-05-10 | 中粮集团有限公司 | Detection method for volatile components of sugar |
| CN108918724A (en) * | 2018-08-30 | 2018-11-30 | 上海应用技术大学 | A kind of detection method of Synthetic Leather characteristic odor |
-
2013
- 2013-03-29 CN CN 201310108245 patent/CN103163251A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198805A (en) * | 2016-07-12 | 2016-12-07 | 中山大学惠州研究院 | A kind of detection method of spices and essence Controlled-Release Capability of Microcapsules |
| CN106645495A (en) * | 2016-12-29 | 2017-05-10 | 中粮集团有限公司 | Detection method for volatile components of sugar |
| CN108918724A (en) * | 2018-08-30 | 2018-11-30 | 上海应用技术大学 | A kind of detection method of Synthetic Leather characteristic odor |
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Application publication date: 20130619 |