CN103159314A - Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method - Google Patents
Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method Download PDFInfo
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- CN103159314A CN103159314A CN2013101029680A CN201310102968A CN103159314A CN 103159314 A CN103159314 A CN 103159314A CN 2013101029680 A CN2013101029680 A CN 2013101029680A CN 201310102968 A CN201310102968 A CN 201310102968A CN 103159314 A CN103159314 A CN 103159314A
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Abstract
The invention discloses a method for eliminating antibiotics resistance gene pollution in water by a hydrogen peroxide coupled ultrasonic method and belongs to the technical field of water treatment. The method provided by the invention is used for solving the problem that the water is polluted by the antibiotics resistance gene. The method is characterized in that the method of feeding hydrogen peroxide into water in combination with ultrasonic radiation is employed so that the antibiotics resistance gene is broken from the point of chemical reaction and basic groups, riboses and phosphoric acids in the antibiotics resistance gene are oxidized by means of strong oxidizing property of the hydrogen peroxide, and consequently, the chemical nature of the antibiotics resistance gene is changed and has no ability of storing and transferring genetic information, on one hand, and on the other hand, many cavitation bubbles are formed in water by means of ultrasonic. When the cavitation bubbles are broken, strong physical destruction effect is generated on the antibiotics resistance gene in water so that the double helix structure of the gene is decomposed and the nucleotide chain of the gen is broken; as a result, the genetic information contained in the nucleotide chain cannot be transferred; therefore, the purpose of eliminating the antibiotics resistance gene pollution is achieved.
Description
Technical field
The invention belongs to water treatment field, relate in particular to a kind of method that in water body, antibiotics resistance gene pollutes of eliminating.
Background technology
In China, the pollution of antibiotics resistance gene is on the rise.Antibiotics resistance gene refers to, can make microorganism the antibiotics material be produced the gene of resistance.In a microbial population, not all individuality has antibiotics resistance gene, because this genoid often produces by sudden change, so gene frequency is lower.But in containing a large amount of antibiotic environment, the probability of microorganisms antibiotics resistance gene increases greatly.Studies confirm that, microorganism can be discharged into the antibiotics resistance gene in health in the middle of environment.Be present in antibiotics resistance gene in environment and can enter by number of ways again and originally microbiotic is not had in the microbe of resistance, make it have the antibiotic ability of opposing.This will be to the mankind's life and the great hidden danger of healthy and safe generation.
Ultrasonic wave refers to frequency higher than the sound wave of 20000 hertz, its good directionality, and penetration power is strong, is easy to the acoustic energy that obtains to concentrate, in the water transmission distance, can be used for finding range, tests the speed, cleans, welding, rubble, sterilization, sterilization etc.Important application is arranged on medical science, military affairs, industry, agricultural.Ul-trasonic irradiation can produce a large amount of small bubbles when liquid, its origin cause of formation has two: one, and in liquid, the part tensile stress occurs and forms negative pressure, and the reduction of pressure makes the dissolved gas supersaturation that originally is dissolved in liquid, and overflows from liquid, becomes small bubbles; The 2nd, powerful tensile stress " is torn liquid ", forms cavity one by one, is called cavitation.Being liquid vapors or the gas that is dissolved in liquid in the cavity, may be even vacuum.The small bubbles that form because of cavatition can constantly move, grow up or collapse with the vibration of surrounding medium and go out.When cavitation bubble collapses when going out, surrounding liquid pours bubble suddenly and produces high temperature, high pressure, produces simultaneously strong pressure pulses.
In view of traditional sewage treatment process not only is difficult to remove antibiotics resistance gene, sewage disposal plant effluent also is considered to the important sources of antibiotics resistance gene in surface water, underground water and soil.Therefore, the antibiotics resistance gene that uses novel treatment process to eliminate in water body pollutes, and is the important step of guaranteeing water quality safety, environmental safety and ecological safety.
Summary of the invention
The present invention will solve this problem of antibiotics resistance gene polluted-water, uses hydrogen peroxide coupling ultrasonic wave radiation to eliminate the method that in water body, antibiotics resistance gene pollutes and provide.
The present invention uses the hydrogen peroxide coupling ultrasonic to eliminate the method that in water body, antibiotics resistance gene pollutes, and realizes by following steps:
(1), the water body that will contain antibiotics resistance gene enters coagulative precipitation tank, uses coagulating agent and flocculation agent to carry out coagulating sedimentation to it and processes, the dosing ratio of coagulating agent and water body is 0.5 gram/ton ~ 500 gram/tons.For the water body of turbidity lower than 20NTU, can directly enter next step without this step;
(2), the sewage that will discharge from settling tank, add the medicament that contains hydrogen peroxide, making the content that adds hydrogen peroxide in rear every cubic metre of water body is 10 gram/tons ~ 10 kg/ton;
(3), added the sewage of hydrogen peroxide to carry out Ultrasonic Radiation to process, ultrasonic wave acoustic energy density is 1 kilowatt/ton ~ 100 kilowatts/ton, the ul-trasonic irradiation time is 0.5min ~ 60min, ultrasonic frequency is 20kHz ~ 40kHz.
Beneficial effect of the present invention:
(1), the antibiotics resistance gene in water body is under the dual function of hydrogen peroxide and Ultrasonic Radiation, on the one hand, hydrogen peroxide has extremely strong oxidisability, base (VITAMIN B4, guanine, cytosine(Cyt), thymus pyrimidine, uridylic), ribose and the phosphoric acid of meeting oxidation antibiotics resistance gene, the chemical nature of antibiotics resistance gene is changed, thereby do not have the ability that stores and convey hereditary information; On the other hand, ultrasonic wave penetration power in water body is strong, propagation distance is far away, can form cavitation bubble one by one in water body.When cavitation bubble collapses when going out, surrounding liquid pours bubble suddenly and produces high temperature, high pressure, produce simultaneously strong pressure pulses, antibiotics resistance gene in water body is produced very strong physical damage effect, make its double-spiral structure disintegration, nucleotide chain fracture, lose the integrity of its genetic information that comprises, the genetic information that wherein comprises can't be transmitted, eliminate antibiotics resistance gene pollution this purpose thereby reach;
(2), the superoxol wide material sources, easy to use, operational condition is good, oxidisability is strong, obvious processing effect;
(3), the Ultrasonic Radiation treatment process has the advantage that equipment is simple, processing efficiency is high, energy consumption is low, and the treatment process of antibiotics resistance gene is simplified, cost of disposal reduces.
Embodiment
Embodiment 1:
Eliminating based on hydrogen peroxide coupling ultrasonic wave radiation the method that in water body, antibiotics resistance gene pollutes follows these steps to carry out: one, water sampling 5L from Xiamen City Jimei District sewage work second pond, by pcr amplification, detect following eight kinds of antibiotics resistance genes: integron genes
int1,
int2, the sulfamido resistant gene
sul1,
sul2,
sulA, the tetracyclines resistant gene
tetA、
tetE、
tetM； At first add coagulant polymeric aluminium chloride 4mg/L in this water sample, precipitation 30min makes its turbidity lower than 20NTU; Two, the water outlet that obtains in step 1, adding wherein massfraction is 30% superoxol 50g, meanwhile, use ultrasonic cell disruptor to carry out supersound process to it, ultrasonic frequency is 30kHz, and ultrasonic wave acoustic energy density is 80W/L, processes 30min; Three, the water outlet water sample that obtains in step 2 is carried out pcr amplification, detect the wherein concentration of antibiotics resistance gene.Result is as shown in the table:
Integron genes in water outlet (
int1,
int2), the sulfamido resistant gene (
sul1,
sul2,
sulA), the tetracyclines resistant gene (
tetA、
tetE、
tetM) concentration such as following table:
Claims (4)
1. the hydrogen peroxide coupling ultrasonic is eliminated the method that in water body, antibiotics resistance gene pollutes, and it is characterized in that: by the coupling ultrasonic wave radiation with add the medicament that contains hydrogen peroxide, reach the purpose of removing antibiotics resistance gene in water body.
2. the hydrogen peroxide coupling ultrasonic is eliminated the method that in water body, antibiotics resistance gene pollutes, and it is characterized in that: realize by following concrete steps:
(1), the water body that will contain antibiotics resistance gene enters coagulative precipitation tank, uses coagulating agent to carry out coagulating sedimentation to it and processes, the dosing ratio of coagulating agent and water body is 0.5 gram/ton ~ 500 gram/tons; For the water body of turbidity lower than 20NTU, can directly enter next step without this step;
(2), the sewage that will discharge from settling tank, add the medicament that contains hydrogen peroxide, making the content that adds hydrogen peroxide in rear water body is 10 gram/tons ~ 10 kg/ton;
(3), added the sewage of hydrogen peroxide to carry out Ultrasonic Radiation to process, ultrasonic wave acoustic energy density is 1 kilowatt/ton ~ 100 kilowatts/ton, the ul-trasonic irradiation time is 0.5min ~ 60min, ultrasonic frequency is 20kHz ~ 40kHz.
3. hydrogen peroxide coupling ultrasonic as claimed in claim 1 is eliminated the method that in water body, antibiotics resistance gene pollutes, and it is characterized in that: the coagulating agent described in step 1 comprises following material: Tai-Ace S 150, iron trichloride, ferrous sulfate, potassium aluminium sulfate, sodium aluminate, ferric sulfate, kaolin, polymerize aluminum chloride, bodied ferric sulfate.
4. hydrogen peroxide coupling ultrasonic as claimed in claim 1 is eliminated the method that in water body, antibiotics resistance gene pollutes, and it is characterized in that: the medicament that contains hydrogen peroxide described in step 2 comprises following medicament: hydrogen peroxide, SPC-D.
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| CN2013101029680A CN103159314A (en) | 2013-03-28 | 2013-03-28 | Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method |
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| CN2013101029680A CN103159314A (en) | 2013-03-28 | 2013-03-28 | Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103466745A (en) * | 2013-08-27 | 2013-12-25 | 清华大学 | Method for facilitating removal of radiation of sulfanilamide antibiotic in waste water by utilizing ferrous ions |
| CN104628234A (en) * | 2015-02-03 | 2015-05-20 | 同济大学 | Method for reducing resistance gene release in anaerobic sludge treatment process |
| CN106082511A (en) * | 2016-08-16 | 2016-11-09 | 华南理工大学 | A kind of degree of depth removes the method for antibiotic in water body |
| CN106830179A (en) * | 2017-03-03 | 2017-06-13 | 河南理工大学 | A kind of method of degrading azoic dye waste water |
| CN109231367A (en) * | 2018-09-06 | 2019-01-18 | 浙江大学 | It is a kind of it is effective degradation citrus processing wastewater in drug resistant gene method |
| CN109602761A (en) * | 2018-12-04 | 2019-04-12 | 华南理工大学 | Application of the kaolinite as antibiotics resistance gene inhibitor |
| CN110803756A (en) * | 2019-11-19 | 2020-02-18 | 济南大学 | Preparation of novel composite water treatment oxidant and method for degrading arsenic-containing feed additive in livestock and poultry breeding wastewater |
| CN111620493A (en) * | 2020-07-03 | 2020-09-04 | 中国农业科学院农业环境与可持续发展研究所 | Method and special equipment for removing antibiotic resistant bacteria and resistant genes in sewage |
| CN111635262A (en) * | 2020-06-05 | 2020-09-08 | 北京紫光英力化工技术有限公司 | Degradation method of livestock and poultry manure antibiotic |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102285703A (en) * | 2011-06-15 | 2011-12-21 | 南京信息工程大学 | Method for ultrasonic removal of sulfadiazine in water |
-
2013
- 2013-03-28 CN CN2013101029680A patent/CN103159314A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102285703A (en) * | 2011-06-15 | 2011-12-21 | 南京信息工程大学 | Method for ultrasonic removal of sulfadiazine in water |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103466745B (en) * | 2013-08-27 | 2015-08-19 | 清华大学 | A kind of method utilizing ferrous ion to promote sulfa antibiotics removal by radiation in waste water |
| CN103466745A (en) * | 2013-08-27 | 2013-12-25 | 清华大学 | Method for facilitating removal of radiation of sulfanilamide antibiotic in waste water by utilizing ferrous ions |
| CN104628234A (en) * | 2015-02-03 | 2015-05-20 | 同济大学 | Method for reducing resistance gene release in anaerobic sludge treatment process |
| WO2016123976A1 (en) * | 2015-02-03 | 2016-08-11 | 同济大学 | Method for reducing release of resistance gene during anaerobic treatment of sludge |
| CN106082511A (en) * | 2016-08-16 | 2016-11-09 | 华南理工大学 | A kind of degree of depth removes the method for antibiotic in water body |
| CN106830179B (en) * | 2017-03-03 | 2020-09-18 | 河南理工大学 | Method for degrading azo dye in wastewater |
| CN106830179A (en) * | 2017-03-03 | 2017-06-13 | 河南理工大学 | A kind of method of degrading azoic dye waste water |
| CN109231367A (en) * | 2018-09-06 | 2019-01-18 | 浙江大学 | It is a kind of it is effective degradation citrus processing wastewater in drug resistant gene method |
| CN109602761A (en) * | 2018-12-04 | 2019-04-12 | 华南理工大学 | Application of the kaolinite as antibiotics resistance gene inhibitor |
| CN109602761B (en) * | 2018-12-04 | 2020-12-22 | 华南理工大学 | Application of kaolinite as antibiotic resistance gene inhibitor |
| CN110803756A (en) * | 2019-11-19 | 2020-02-18 | 济南大学 | Preparation of novel composite water treatment oxidant and method for degrading arsenic-containing feed additive in livestock and poultry breeding wastewater |
| CN111635262A (en) * | 2020-06-05 | 2020-09-08 | 北京紫光英力化工技术有限公司 | Degradation method of livestock and poultry manure antibiotic |
| CN111620493A (en) * | 2020-07-03 | 2020-09-04 | 中国农业科学院农业环境与可持续发展研究所 | Method and special equipment for removing antibiotic resistant bacteria and resistant genes in sewage |
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Application publication date: 20130619 |