CN103156600A - Brain slice experiment meter - Google Patents
Brain slice experiment meter Download PDFInfo
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- CN103156600A CN103156600A CN2011104222549A CN201110422254A CN103156600A CN 103156600 A CN103156600 A CN 103156600A CN 2011104222549 A CN2011104222549 A CN 2011104222549A CN 201110422254 A CN201110422254 A CN 201110422254A CN 103156600 A CN103156600 A CN 103156600A
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- incubation
- incubation slot
- experiment
- beaker
- brain sheet
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Abstract
The invention relates to the field of medical experiments, and discloses a brain slice experiment meter for a patch clamp brain slice experiment. The brain slice experiment meter for the patch clamp brain slice experiment comprises an incubation groove, a fixing device and an air-connecting tube fixing part, and is characterized by comprising the incubation groove fixed inside a beaker, wherein a plurality of incubation holes are arranged inside the incubation groove, the bottom surfaces of the incubation holes are yarn meshes, the incubation groove tightly contacts with the inner lateral wall of the beaker through the fixing device, and the air-connecting tube fixing part is fixed outside the incubation groove. The brain slice experiment meter well solves the situations that because mixed gas flow control is not good, brain slices are damaged and accumulated, and therefore brain slice incubation is undesirable, and the experiment is affected.
Description
Technical field
The present invention relates to the medical experiment field, brain sheet experiment instrument used when being the experiment of patch-clamp brain sheet.
Background technology
In medical science electrophysiology experiment field, use patch-clamp to record the experiment of brain sheet electrophysiology signal very common.Such as: Parkinsonian research, epilepsy experiment, the research of cental system ion channel, be administered systemically experiment and drug dependence experiment etc. all need to hatch good brain sheet as obtaining the fundamental prerequisite of stablizing experimental result.The self-control macropore incubation slots that adopt were tested more in the past.But in practical situation, often occur because air feed is uneven, the phenomenon that the brain sheet that causes damages and piles up, and then the situation that the brain sheet is hatched the harmful effect experiment appears.
Summary of the invention
The purpose of this invention is to provide a kind of equipment of conveniently hatching the brain sheet, it is brain sheet experiment instrument, it has solved preferably, and the not good brain sheet that causes damages, accumulation because mixed gas flow is controlled, and then the situation that the brain sheet is hatched harmful effect experiment appears, it can be hatched the brain sheet respectively, and the balance air feed, so just delayed the downright bad speed of brain sheet, be beneficial to the expansion of experiment.
Technical scheme of the present invention is: a kind of brain sheet experiment instrument is provided, it is characterized in that: it comprises the incubation slot that is fixed in beaker, and a plurality of holes of hatching are arranged in incubation slot, and hatching the bottom surface, hole is gauze.
Described incubation slot is fixed with the beaker medial wall by holder, and incubation slot outer side is fixed with the trachea fixture.
Described incubation slot is that rustless steel is made, and its bottom surface is fixed with the rustless steel gauze, and incubation slot is evenly distributed with a plurality of holes of hatching.
Described holder is by bolt and have the leather packing spiral of internal thread hole to connect and compose, and bolt welding is on the incubation slot lateral surface, and leather packing withstands on the beaker inwall.
Described trachea fixture is welded on the incubation slot lateral surface, and it is a foil by nut scalable hole clipping size, is used for the air inlet pipe of card different tube diameters.
The invention has the beneficial effects as follows: a plurality of holes of hatching are arranged in incubation slot, and its bottom surface is connected with gauze, and with beaker medial wall close contact, incubation slot outer side is fixed with the trachea fixture to incubation slot by holder.It has solved preferably, and the not good brain sheet that causes damages, accumulation because mixed gas flow is controlled, and then the situation that the brain sheet is hatched the harmful effect experiment appears, it can be hatched the brain sheet respectively, and the balance air feed, so just delay the downright bad speed of brain sheet, be beneficial to the expansion of experiment.
Description of drawings
The present invention is further illustrated below in conjunction with drawings and Examples.
Fig. 1 is the top view of embodiment 2 brain sheet experiment instruments;
Fig. 2 is the normotopia view of embodiment 2 brain sheet experiment instruments;
Fig. 3 is the top view of embodiment 2 holders;
Fig. 4 is the normotopia cross sectional view of embodiment 2 holders;
Fig. 5 is the top view of embodiment 2 trachea fixtures.
In figure: 1, beaker; 2, incubation slot; 3, holder; 4, trachea fixture; 5, hatch the hole; 6, gauze; 7, bolt; 8, leather packing; 9, nut; 10, foil.
The specific embodiment
This brain sheet experiment instrument, it comprises the incubation slot 2 that is fixed in beaker 1, and a plurality of holes 5 of hatching are arranged in incubation slot 2, hatching 5 bottom surfaces, hole is gauzes 6.The mode that incubation slot 2 is fixed on beaker 1 can make various forms.
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, shown in Figure 5, brain sheet experiment instrument is made of a beaker 1, an incubation slot 2, three holders 3 and trachea fixture four parts; Incubation slot 2 can be made by stainless steel material, and it has the identical even distribution of a plurality of sizes to hatch hole 5, and there is gauze 6 its bottom surface, and gauze 7 can be the bottom surface of incubation slot 2 integrative-structures, also can be fixed on to hatch 5 bottom surfaces, hole.On holder 3, threaded bolt 7 is welded on incubation slot 2, the leather packing 8 of built-in screw thread withstands on beaker 1 inwall with fixing incubation slot 2, because holder 3 can and have leather packing 8 spirals of internal thread hole to connect and compose by bolt 7, it can regulate collapsing length according to beaker 1 internal diameter, so can be with incubation slot 2 fixedly in the beaker 1 of different-diameter.
As shown in Fig. 1,2,5, trachea fixture 4 is stainless steel material, and trachea fixture 4 is welded on incubation slot 2 lateral surface, and it is a foil 10 by nut 9 scalable hole clipping sizes, is used for the air inlet pipe of card different tube diameters.
The above; only for the specific embodiment of preferred embodiment of the present invention; the present invention is not limited to this embodiment; for those skilled in the art; carry out the interpolation of technical characterictic and replace all belonging to scope of the present invention in the category that the present invention provides; therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.
Claims (5)
1. brain sheet experiment instrument is characterized in that: it comprises the incubation slot (2) that is fixed in beaker (1), and a plurality of holes (5) of hatching are arranged in incubation slot (2), and hatching bottom surface, hole (5) is gauze (6).
2. brain sheet experiment instrument according to claim 1 is characterized in that: described incubation slot (2) is fixed by the same beaker of holder (3) (1) medial wall, and incubation slot (2) outer side is fixed with trachea fixture (4).
3. brain sheet experiment instrument according to claim 1, it is characterized in that: described incubation slot (2) is that rustless steel is made, and its bottom surface is fixed with rustless steel gauze (6), and incubation slot (2) is evenly distributed with a plurality of holes (5) of hatching.
4. brain sheet experiment instrument according to claim 1, it is characterized in that: described holder (3) is by bolt (7) and have leather packing (8) spiral of internal thread hole to connect and compose, bolt (7) is welded on incubation slot (2) lateral surface, and leather packing (8) withstands on beaker (1) inwall.
5. brain sheet experiment instrument according to claim 1, it is characterized in that: described trachea fixture (4) is welded on incubation slot (2) lateral surface, it is a foil (10) by nut (9) scalable hole clipping size, is used for the air inlet pipe of card different tube diameters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104222549A CN103156600A (en) | 2011-12-16 | 2011-12-16 | Brain slice experiment meter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104222549A CN103156600A (en) | 2011-12-16 | 2011-12-16 | Brain slice experiment meter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103156600A true CN103156600A (en) | 2013-06-19 |
Family
ID=48580500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104222549A Pending CN103156600A (en) | 2011-12-16 | 2011-12-16 | Brain slice experiment meter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103156600A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050002985A1 (en) * | 2001-10-30 | 2005-01-06 | Heal Richard David Albert | Device with recessed tracks for forming a cellular network |
| GB2423774A (en) * | 2005-02-17 | 2006-09-06 | Hitachi Ltd | Method and apparatus for controlling the morphological growth of neurons |
| CN201952434U (en) * | 2011-01-28 | 2011-08-31 | 颜峰 | Cell culture device |
| US20110269172A1 (en) * | 2010-04-28 | 2011-11-03 | Katholieke Universiteit Leuven, K.U. Leuven R&D | Micro-Electrode Grid Array for Top and Bottom Recording from Samples |
| CN202458366U (en) * | 2011-12-16 | 2012-10-03 | 马劼 | Novel brain slice incubation instrument |
-
2011
- 2011-12-16 CN CN2011104222549A patent/CN103156600A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050002985A1 (en) * | 2001-10-30 | 2005-01-06 | Heal Richard David Albert | Device with recessed tracks for forming a cellular network |
| GB2423774A (en) * | 2005-02-17 | 2006-09-06 | Hitachi Ltd | Method and apparatus for controlling the morphological growth of neurons |
| US20110269172A1 (en) * | 2010-04-28 | 2011-11-03 | Katholieke Universiteit Leuven, K.U. Leuven R&D | Micro-Electrode Grid Array for Top and Bottom Recording from Samples |
| CN201952434U (en) * | 2011-01-28 | 2011-08-31 | 颜峰 | Cell culture device |
| CN202458366U (en) * | 2011-12-16 | 2012-10-03 | 马劼 | Novel brain slice incubation instrument |
Non-Patent Citations (1)
| Title |
|---|
| DIANA M. MATHIS1 ETC.: "Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology", 《JOURNAL OF VISUALIZED EXPERIMENTS》 * |
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Application publication date: 20130619 |