CN103155771A - Method of using red leaf vegetable to degrade diesel pollutants in soil - Google Patents
Method of using red leaf vegetable to degrade diesel pollutants in soil Download PDFInfo
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- CN103155771A CN103155771A CN 201310079291 CN201310079291A CN103155771A CN 103155771 A CN103155771 A CN 103155771A CN 201310079291 CN201310079291 CN 201310079291 CN 201310079291 A CN201310079291 A CN 201310079291A CN 103155771 A CN103155771 A CN 103155771A
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Abstract
The invention relates to a method of using a red leaf vegetable to degrade diesel pollutants in soil. The method of using the red leaf vegetable to degrade the diesel pollutants in soil specifically comprises the following steps: red leaf vegetable seedlings are planted in soil polluted by diesel, planting density is 200/square meter, and greasy dirt pollution degree is 5000-20000 mg/kg. After 104 days, sampling is conducted in a destructive mode and data is analyzed on the function effect. The method of using the red leaf vegetable to degrade the diesel pollutants in soil has the advantages that good capability of removing the diesel pollutants in the soil is possessed, removal rate of the diesel pollutants in the soil can reach 79.8% after104 days, soil physiochemical properties are not destroyed, secondary pollution does not exist, technology is reliable, operation is convenient, cost is low, high tolerance and total petroleum hydrocarbon (TPH) degradation rate are possessed on the soil polluted by the diesel, appearance of the red leaf vegetable is attractive, not only environment is beautified, but also certain economic and ecological benefits are possessed. Therefore, the method of using the red leaf vegetable to degrade the diesel pollutants in soil can be applied to phytoremediation of large area diesel polluting soil environment and greening and beautifying of greasy environment.
Description
Technical field
The present invention relates to a kind of biological renovation method of petroleum-type contaminated soil, particularly a kind of method of utilizing contaminated by diesel oil thing in wetland plant red autumnal leaves dish degraded soil.
Background technology
Oil is one of most important fuel and industrial chemicals in traffic, life, industry, and is most important in fields such as communications and transportation and Chemical Manufacture.In the large scale mining of oil, smelting, transportation, use and processing procedure, the Frequent Accidents such as pollution, omission, blowout, Pipeline Leak, direct or indirect polluted-water, contaminated soil cause huge pollution to destroy to the ecotope of water body and wetland.The constituent of oil comprises linear paraffin, branched paraffin, aromatic hydrocarbon, cycloalkane and other additives etc., enter meeting spoiled soil structure in soil, change soil enzyme activities and biological community structure, finally cause soil desertification, salinization of soil and desertification.In oil, contained polycyclic aromatic hydrocarbon (PAHs) has three and causes effect, can enter human body by various direct or indirect approach such as direct contact or food chains, is detrimental to health.
At present, abiotic method and biological method are adopted in the processing that petroleum-type is polluted, and abiotic method is processed effective for emergent oil spill event, but disposal cost is high, labor intensive, financial resources.Phytoremediation is utilized plant and rhizospheric microorganism thereof to remove pollutant and is repaired impaired environment, with compare based on traditional restorative procedure of physico-chemical process have that cost is low, the remarkable advantage such as non-secondary pollution, repairing effect are good.At present phytoremediation technology has had many successful application in practice, but selected plant mostly is herbage and reed class, also is in weak link for the research of some wetland plant degraded oil pollutant abilities.
Summary of the invention
A kind of method of utilizing contaminated by diesel oil thing in wetland plant red autumnal leaves dish degraded soil that provides is provided.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method of utilizing contaminated by diesel oil thing in wetland plant red autumnal leaves dish degraded soil, the concrete steps that it is characterized in that the method are: red autumnal leaves dish seedling is planted in the soil that is subjected to contaminated by diesel oil, planting density is 200/square metre, and the oil pollution degree is: 5000～20000mg/kg.
Wetland plant red autumnal leaves dish seedling is that a kind of environmental suitability is strong, the wetland plant of easy to raise and cultivation, fast growth.The present invention has found that a kind of wetland plant red autumnal leaves dish has the ability of contaminated by diesel oil thing in good removal soil, can reach 79.8% to the diesel oil type removal of pollutants rate in contaminated environment within 104 days.The present invention chooses the plant red autumnal leaves dish that oil is had the efficient degradation effect, has made up floristic deficiency in wetland soil is repaired, and when beautifying the environment, can also produce certain economic worth and social benefit.
Technical method of the present invention has not spoiled soil physicochemical property, non-secondary pollution, technology is reliable, easy to operate, cost is low and diesel fuel contaminated soil is had the characteristics such as higher tolerance and total petroleum hydrocarbon (TPH) degradation rate, and red autumnal leaves dish profile is beautiful, not only can beautify the environment, and have certain economy and zoology benefit; Therefore this technical method applicable to the greening of the phytoremediation of large tracts of land diesel fuel contaminated soil environment and oil pollution environment, beautify.
Description of drawings
The clearance of diesel oil in Fig. 1 red autumnal leaves dish oil removing experiment contrast group and experimental group soil
Fig. 2 red autumnal leaves dish oil removing experiment contrast group and experimental group FDA hydrolytic enzyme activities
Fig. 3 red autumnal leaves dish oil removing experiment contrast group and experimental group catalase activity
Fig. 4 red autumnal leaves dish oil removing experiment contrast group and experimental group sucrase active
Fig. 5 red autumnal leaves dish oil removing experiment contrast group and experimental group polyphenol oxidase activity
Control group in figure is for planting red autumnal leaves dish: CK0, CK1, CK2, CK3, and diesel oil concentration is respectively 0,5000,10000, the 20000mg/kg dry ground; Experimental group is plantation red autumnal leaves dishes: HY1, HY2, HY3, diesel oil concentration is respectively 0,5000,10000, the 20000mg/kg dry ground.
Embodiment
Below in conjunction with example and accompanying drawing, the present invention is further detailed.
One, material is prepared
1, wetland plant source and soil collecting
(1) wetland plant source: the wetland plant seedling is available from Shanghai University's Golconda school district greenhouse;
(2) soil collecting: pollution-free soil picks up from school district, east, Shanghai University's Golconda school district, crosses 2 mm sieves after natural air drying, is kept under 4 ℃ stand-by.The basic physical and chemical of experiment soil: pH value, 8.3; Sand grains, 32.2%; Powder, 60.4%; Clay, 7.4%; Organic matter, 19.6 g/kg.
2, for examination diesel oil
0
#Diesel oil (density 0.84 mg/L; 170 ~ 370 ℃ of boiling points; Cetane number 〉=45%; And the gas station bright available from Baoshan District, Shanghai of carbon number C10 ~ C22).
3, experimental instrument and equipment
Constant temperature oscillator, steam sterilization pan, 755 type spectrophotometers, ultrasonic washing instrument, pH meter, DK-8D type electric heating constant temperature tank (the gloomy reliable Instr Ltd. that tests in Shanghai); YXJ-2 centrifuge (Hunan instrument centrifuge instrument Co., Ltd).
Two, the application method of contaminated by diesel oil thing in wetland plant red autumnal leaves dish efficient degradation soil
1, the plantation of wetland plant red autumnal leaves dish
Mix with the air-dry pollution-free soil that sieves after with carrene, appropriate diesel oil being dissolved, being configured to diesel oil concentration is the dirty soil of 5000,10000 and 20000 mg/kg concentration, and it is clean that placement makes the carrene volatilization one week in fume hood.Under each diesel oil concentration, control group and experimental group are set.Take the 2 dirty soil of kg and be placed in test with flowerpot (diameter 20 cm, high 25 cm), plant 3 ~ 4 wetland seedling in the every basin of experimental group, every group establish 3 parallel.Experimental session is placed in plant indoor, and mean temperature is 6 ℃ ~ 15 ℃, and natural lighting is irrigated maintenance soil moisture with running water and is about 35%.
2, soil collecting and preservation
Plant growth gathers respectively the Rhizosphere Soil (apart from the root surface less than 0.5 cm) of every Plants and analyzes after 104 days, gather top layer 1 ~ 5 cm soil sample without the plant treatment group.Soil sample is kept at after with the freeze-dryer drying preserves under-80 ℃ until analyze.
3, Determination of Diesel Oil analysis in soil
Utilize gravimetric method that diesel oil residual in soil sample is analyzed.Get soil sample after 5 g drying and screening in 50 mL conical flasks, extractant (carrene/n-hexane 1:1, v/v) the ultrasonic extraction 20 min(45 kHz in ultrasonic washing instrument that add 30 mL, 300 W, 25 ℃), then at 4000r/min centrifugation 5 min.Repeat twice, supernatant is collected in the small beaker of known weight of constant weight, beaker is placed on makes solvent evaporate into constant weight fully in fume hood, the weightening finish of small beaker is residual Determination of Diesel Oil.
4, soil enzyme activities analysis
Choose the FDA(fluorescein(e) diacetate) hydrolase, catalase, invertase, these 4 kinds of important soil enzymes of polyphenol oxidase analyze soil to the activity of redox ability, Cycle of nutrients ability and the edaphon of pollutant.
Adopt spectrophotometry FDA hydrolytic enzyme activities.Take 1 g soil sample, put into the phosphate buffer of pH=7.6 after 30 mL sterilizations, disperse 15 min in 30 ℃, the constant temperature oscillator of 200 r/min, add afterwards FDA solution 1 mL of 2 g/L, colour developing 2 h, measure absorbance in 490 nm wavelength places in constant temperature oscillator.
Catalase activity adopts permanganimetric method, and sucrase active adopts dinitrosalicylic Acid Colorimetry, and polyphenol oxidase activity is measured with the pyrogallol development process.
Three, the application result of contaminated by diesel oil thing in wetland plant red autumnal leaves dish efficient degradation soil
Control group and experimental group are provided with respectively three Duplicate Samples, and following data analysis is all to adopt mean value and standard deviation to represent.
After experiment was carried out 104 days, in experimental group and control group, the clearance of contaminated by diesel oil thing as shown in Figure 1.When diesel oil concentration was 20000 mg/kg, the red autumnal leaves dish can significantly promote Diesel degradation, and after 104 days, degradation rate has reached 79.8%, apparently higher than the blank group.This is because the red autumnal leaves dish toxicity of enduring high-concentration diesel oil preferably when diesel oil concentration improves, can stimulate the root rhizosphere secreta of red autumnal leaves dish, impels the microorganism in soil to make active response to Diesel degradation.
Fig. 2 is the FDA hydrolytic enzyme activities in soil in control group and experimental group.The activity of FDA hydrolase represents the gross activity of Soil Microorganism, 5000, under 20000mg/kg diesel oil concentration, in red autumnal leaves dish Rhizosphere Soil, microbial activity is all apparently higher than non-Rhizosphere Soil, and the growth of red autumnal leaves dish has promoted the growth activity of Soil Microorganism group.
Fig. 3 is the catalase activity of control group and experimental group.Catalase is that the hydrolysis hydrogen peroxide makes its catalyzing enzyme that is decomposed into water and oxygen, can reduce hydrogen peroxide to the toxic action of organism.5000, under 10000mg/kg diesel oil concentration, in plant rhizosphere soil, catalase activity is a little less than control group, but difference is not remarkable.Under 20000mg/kg diesel oil concentration, in plant rhizosphere soil, catalase activity is a little more than control group, and in the contaminated by diesel oil thing soil of high concentration, wetland plant red autumnal leaves dish has improved catalatic activity in soil.
Fig. 4 is control group and experimental group sucrase active, and invertase has material impact to the circulation of carbon in soil.5000, under 10000mg/kg diesel oil concentration, wetland plant red autumnal leaves dish can improve sucrase active, and the metabolic activity of red autumnal leaves dish rhizosphere soluble carbon is higher.
Fig. 5 is control group and experimental group polyphenol oxidase activity, and polyphenol oxidase is a kind of oxidoreductase, can act on catechol, and it is oxidized to adjacent benzene diquinone, is requisite catalyzing enzyme in the degrading polycyclic aromatic hydrocarbons process.In the plant rhizosphere soil of wetland plant red autumnal leaves dish, polyphenol oxidase activity significantly increases than blank group, and in wetland plant red autumnal leaves dish rhizosphere, the degradation rate of phenols is higher.
Wetland plant red autumnal leaves dish can efficient degradation soil in the contaminated by diesel oil thing, particularly under the diesel oil concentration of high-load, the red autumnal leaves dish can improve the degraded to diesel oil significantly, in 20000mg/kg soil, the red autumnal leaves dish can reach 79.8% to the degradation rate of diesel oil, has the function of degrading high concentration diesel fuel contaminated soil preferably.
The present invention is significant for the control of increasing oil pollution disaster, in the diesel oil type pollutant in removing environment, can also reach the effect of beautifying the environment, and has certain economic worth, social value and landscape effect.
Claims (1)
1. method of utilizing contaminated by diesel oil thing in wetland plant red autumnal leaves dish degraded soil, the concrete steps that it is characterized in that the method are: red autumnal leaves dish seedling is planted in the soil that is subjected to contaminated by diesel oil, planting density is 200/square metre, and the oil pollution degree is: 5000～20000mg/kg.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105160560A (en) * | 2015-09-30 | 2015-12-16 | 谢乐 | Method for benefit analysis by using electricity reward coupon mechanism |
| CN106269849A (en) * | 2016-09-22 | 2017-01-04 | 北京新源环境有限公司 | A kind of plant bionic and the method for plant combined repairing polluted soil |
-
2013
- 2013-03-13 CN CN 201310079291 patent/CN103155771A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105160560A (en) * | 2015-09-30 | 2015-12-16 | 谢乐 | Method for benefit analysis by using electricity reward coupon mechanism |
| CN106269849A (en) * | 2016-09-22 | 2017-01-04 | 北京新源环境有限公司 | A kind of plant bionic and the method for plant combined repairing polluted soil |
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Application publication date: 20130619 |