CN103153083A - Method for producing high-protein-content organic material, method for producing feed, and feed - Google Patents
Method for producing high-protein-content organic material, method for producing feed, and feed Download PDFInfo
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- CN103153083A CN103153083A CN2011800483660A CN201180048366A CN103153083A CN 103153083 A CN103153083 A CN 103153083A CN 2011800483660 A CN2011800483660 A CN 2011800483660A CN 201180048366 A CN201180048366 A CN 201180048366A CN 103153083 A CN103153083 A CN 103153083A
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
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- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Botany (AREA)
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- Sustainable Development (AREA)
- Biochemistry (AREA)
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- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
The phorbol ester is decomposed and removed from the organic matter containing phorbol ester at low cost and with high processing ability, and the organic matter containing high protein is produced. Mixing organic matter containing phorbol ester with Bacillus bacteria, and fermenting to decompose phorbol ester in the organic matter. In this case, it is preferable that the phorbol ester-containing organic material is mixed with the Bacillus bacteria and is subjected to preculture, then the phorbol ester-containing organic material is mixed with the Bacillus bacteria, and the preculture product is added to the mixture to perform main fermentation, thereby decomposing the phorbol ester in the organic material.
Description
Technical field
The present invention relates to by from containing Buddhist ripple ester (phorbol ester) thus organic matter remove the method that Buddhist ripple ester is made organic matter and the feed contain high protein.
Background technology
Processing method as the environmental pollutants contained take the processing process of animal feed and feed material as object, in the raw material of removing feed or feed material and toxicant form as purpose has disclosed method in patent documentation 1 and patent documentation 2 for example as typical example.
In patent documentation 1, the technology of record is, add fatty acid ester and fatty acid amide, free fatty and hydro carbons as the volatility working fluid in the fat that contains environmental pollutants and toxic component or oil, then, by fat or oil are implemented the stripping processing together with the volatility working fluid, environmental pollutants or toxic component are separated from fat or oil together with the volatility working fluid.In addition, it is following facture that stripping is processed: be blown into steam or gas in the liquid that contains the predetermined substance of wanting to remove, make its volatilization after perhaps sneaking into the high liquid of volatility, be perhaps vacuum condition by making whole liquid, make specific material to steam or gas phase or volatile fluid phase shift, perhaps make predetermined substance volatilization itself, thereby remove specific material from liquid.
In patent documentation 2, the technology of record is to remove the phytic acid that contains in the cereal as feed or food as purpose.After animal absorbed the feed and food of the phytic acid that contains high concentration, the nutritionally normal absorption of important trace meter class in enteron aisle can be obstructed, and may cause a series of deficiency diseases.Therefore need to remove phytic acid from the cereal that contains the phytic acid as above-mentioned.In this technology, by inoculation aspergillus oryzae and Qu processed in the cereal of the Soybean Meal that contains phytic acid etc., the phytase and the such phytic acid catabolic enzyme of phosphate that utilize aspergillus oryzae to produce in breeding decompose and remove phytic acid in cereal.
Patent documentation 1: No. 3905538 communique of Japan Patent
Patent documentation 2: Japanese patent laid-open 8-214822 communique
Summary of the invention
Invent problem to be solved
Yet in disclosed technology, after having passed through stripping process, the volatility working fluid that contains environmental pollutants or toxic component has residual in patent documentation 1.Because isolate environmental pollutants or toxic component and be not easy from this volatility working fluid, therefore passed through the volatility working fluid after the stripping operation and be difficult to be utilized again.Therefore, carry out the stripping processing at every turn and all need to use new volatility working fluid, meanwhile passed through in the volatility working fluid of stripping process and contained environmental pollutants or toxic component, so need carry out safe handling to it, the operation cost that relates to processing increases and becomes problem.
In addition, the stripping that utilizes in this technology is processed, in fact in carrying out the stripping groove that stripping processes, if the temperature and pressure in incorrect control flume, the supply rate of volatility working fluid etc., the rate of removing of environmental pollutants or toxic component will can not improve.Therefore, realize that these just must need expensive control device and machine, the problem of the initial stage cost up when also having introducing equipment.
In addition, the stripping that utilizes in this technology is processed, if the environmental pollutants in the processing object or toxicant can not fully mix with the volatility working fluid in treatment trough contacts, and can not improve the rate of removing.Therefore, process object and must be confined to fat or the aqueous material of wet goods.So the processing object that has for the solid shapes such as oil meal of Soybean Meal or other plant is difficult to applicable so large problem.
In addition, in the disclosed technology of patent documentation 2, although also go for the processing object of solid shape of the oil meal etc. of Soybean Meal and other plant, that can remove only limits to process phytic acid contained in object.Therefore, when containing such phytic acid catabolic enzyme institute indissoluble other toxic component of phytase that aspergillus oryzae produces and phosphate in breeding in processing object, the problem that existence can not be suitable for.
Here, contain oil component with 30～40% so high containing ratios in the seed of the Jatropha curcas (Jatropha curcas.L.) of Euphorbiaceae, yet contain the Buddhist ripple ester that has as the report of carcinogenic promoting agent (tumor promoter) in this oil, therefore can not be eaten.Therefore, in recent years, as being expected to not the Renewable Energy Resources that can clash with the grain purposes and worldwide being attracted attention.
In addition, when being extracted oil, a large amount of seeds will inevitably produce a large amount of seed oil meals, the protein content of these oil meals accounts for 60%, the protein content (approximately 45%) that is compared to the soybean oil meal of main feedstuff is taller, and the oil meal of jatropha curcas seed has can be as than the soybean oil meal possibility utilized of good feedstuff also.
But, also contain Buddhist ripple ester class in this jatropha curcas seed oil meal, so be difficult in reality as the feedstuff utilization, present situation is or not utilize and discarded method as the method for the lower fertilizer utilization of added value.
Even for to contain the Buddhist ripple ester of removing as its toxic component the organic matter that has as the Buddhist ripple ester of the report of carcinogenic promoting agent be purpose from the same with the jatropha curcas seed oil meal, and disclosed technology in the applicable patent documentation 1 of wish, because the oil meal of jatropha curcas seed is the organic matter of solid shape, therefore the stripping that uses in this technology will fully be removed Buddhist ripple ester in processing, and is also very difficult physically.In addition, in the situation of the applicable patent documentation 2 disclosed technology of wish, because aspergillus oryzae is low to the capacity of decomposition of Buddhist ripple ester, therefore also can't remove fully.
As mentioned above, if will from such the containing of jatropha curcas seed oil meal have as the organic matter of the Buddhist ripple ester of the report of carcinogenic promoting agent with purpose low-cost and that remove fully toxic component Buddhist ripple ester, only can't realize with disclosed technology, need to develop the technology that can realize that above-mentioned purpose is new.
Therefore, the inventor finds by utilizing, Buddhist ripple ester to be had the high de-agglomeration ability and to hold facile microorganism through conscientious research, with low cost and high throughput ground, Buddhist ripple ester is removed from the organic matter that contains Buddhist ripple ester, has completed the present invention.
Namely, purpose of the present invention is for providing manufacture method and the feed of the organic manufacture method that contains high protein, the organic matter that contains high protein, feed, this organic manufacture method that contains high protein is used the bacillus bacterium, Buddhist ripple ester is removed from the organic matter that contains Buddhist ripple ester, can be used aptly as the feed of domestic animal.
The means of dealing with problems
In order to achieve the above object, the organic manufacture method that contains high protein of the present invention is that the organic matter that will contain Buddhist ripple ester belongs to bacterium with bacillus (Bacillus) and mixes and make it fermentation, the method that the Buddhist ripple ester in organic matter is decomposed.
In addition, the organic formation that contains high protein of the present invention is, the organic matter that contains Buddhist ripple ester mixed with the bacillus bacterium and make it fermentation, and the Buddhist ripple ester in organic matter is decomposed.
In addition, the manufacture method of feed of the present invention is the organic matter that contains Buddhist ripple ester is mixed with the bacillus bacterium and make it fermentation, the method that the Buddhist ripple ester in organic matter is decomposed.
In addition, the formation of feed of the present invention is, contains tunning, makes it to ferment after this tunning makes the organic matter that contains Buddhist ripple ester and the bacillus bacterium mixes, Buddhist ripple ester is decomposed obtains.
The invention effect
By the present invention, do not using the high price volatile reagent or do not producing the reluctant waste liquid that contains toxicant, and need not to import expensive control device and control in the situation of machine, just can from having organic matter as the Buddhist ripple ester of the report of carcinogenic promoting agent, such the containing of jatropha curcas seed oil meal remove Buddhist ripple ester.
In addition, make its fermentation by using cheapness and hold facile bacillus bacterium, make the organic matter that contains Buddhist ripple ester under relatively undemanding condition, compare more effectively to decompose during with other microorganism of use and remove Buddhist ripple ester.
Due to these, the present invention can provide when controlling the first current cost relevant with processing, operation cost than the lowland, can also with the efficient of excellence decompose and remove Buddhist ripple ester the organic manufacture method that contains high protein, its organic matter that contains high protein, feed manufacture method with and feed.
Description of drawings
Fig. 1 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of the first embodiment of the present invention and feed.
Fig. 2 is the figure of the productivity ratio of explanation jatropha curcas oil.
Fig. 3 is the figure of output of the oil expression oil meal of explanation Jatropha curcas.
Fig. 4 be explanation jatropha curcas seed core oil expression oil meal, as the figure of the advantage of feedstuff.
Fig. 5 is the figure that shows the Buddhist ripple the rate of breaking-up ester in organic matter.
Fig. 6 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of the second embodiment of the present invention and feed.
Fig. 7 is the figure that shows the variation of the Buddhist ripple ester content in organic matter.
Fig. 8 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of the 3rd embodiment of the present invention and feed.
Fig. 9 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of the 4th embodiment of the present invention and feed.
Figure 10 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of the 5th embodiment of the present invention and feed.
Figure 11 is the figure that shows that the Buddhist ripple ester content in the organic matter of embodiment and comparative example changes.
Figure 12 is the chart that shows that the Buddhist ripple ester content in the organic matter of embodiment and comparative example changes.
Figure 13 show to use the figure of feeding experiment result of chicken of feed of the present invention and the chart that shows the average weight transition.
Figure 14 is figure and the chart that shows the feed intake of the chicken of using feed of the present invention.
The specific embodiment
Below, contain the organic manufacture method of high protein, the organic matter that contains high protein, the manufacture method of feed and preferred embodiment describing of feed with reference to figure to of the present invention.
[the first embodiment]
At first, the formation for the first embodiment of the present invention describes with reference to Fig. 1～Fig. 5.Fig. 1 is the figure of operation that shows the manufacture method of the organic matter that contains high protein of present embodiment and feed.Fig. 2～Fig. 5 is respectively that figure, the figure that shows the Buddhist ripple the rate of breaking-up ester in organic matter, the explanation jatropha curcas seed core oil expression oil meal of output of oil expression oil meal of figure, explanation Jatropha curcas of productivity ratio of oil of explanation Jatropha curcas is as the figure of the advantage of feedstuff.
As shown in Figure 1, the organic matter that contains high protein of present embodiment and the manufacture method of feed, in the operation that at first will stir as the organic matter that contains Buddhist ripple ester of processing object, be stirred well to the distribution that becomes homogeneous together with bacillus (Bacillus) belongs to bacterium.
Then, will be transferred to by the mixture that agitating procedure obtains temperature and be in fermenting cellar or round under management, the fermentation procedure of the specified time limit of fermenting.
After fermentation procedure finished, the mixture after the processing of taking out in fermenting cellar or the round became the organic matter that contains high protein that Buddhist ripple ester is decomposed under the effect of bacillus bacterium.In addition, by the second order effect of bacillus bacterium, the vitamin in the mixture after processing and mineral matter and other components increase.
Here, the what is called organic matter that contains high protein refers to the organic matter that the protein containing ratio is high.Particularly, can be with the protein containing ratio 40～65% or the organic matter more than it be called the organic matter that contains high protein.
Can with as the organic matter that contains high protein that obtains be mixed in livestock feed, perhaps directly use, thereby obtain feed.Be mixed into when using in livestock feed, mixed proportion is had no particular limits, as aftermentioned in embodiment, even for example confirm to add the organic matter that contains high protein of 10 % by weight in feed, also raising chicken successfully.In addition, domestic animal is used mammality and birds dog, cat, pig, ox, horse, sheep, chicken etc., beyond the people and carries out domesticated animal by the people.
The organic matter that contains Buddhist ripple ester that uses in the organic manufacture method that contains high protein as present embodiment can use the oil meal after for example the seed of the Jatropha curcas of Euphorbiaceae (Jatropha curcas L.) extracts oil or use this seed shelling and the oil meal after nucleus of the seed (kernel) oil expression of the inside of taking-up.
Fig. 2 is at the representational various oil crops of cultivation, the relatively figure of oil production between the year of per unit cultivated area all over the world.According to this figure as can be known, the oily output of palm is large highlightedly, is secondly Jatropha curcas.But the zone that can cultivate palm is confined to the more fertile place, the torrid zone that precipitation is enriched, and palm oil has the purposes that can be used as grain.Therefore, palm in large quantities as fuel or industrial use, is become the situation that is difficult to obtain common recognition on world in recent years, with palm oil as regenerated energy resource and expanding production is very difficult.
On the other hand, Jatropha curcas has the high-oil offtake that is only second to palm, owing to containing the Buddhist ripple ester that has as the report of carcinogenic promoting agent in jatropha curcas oil, so edible not.Therefore, can not produce as palm oil and the conflicting of grain purposes.In addition, Jatropha curcas can also can be cultivated in the soil few and dry in precipitation, that the grain alimentary crop does not grow in the rainy tropical area cultivation of energy structure cultivation palm certainly, is therefore paid close attention in the world as regenerated energy resource likely.
Fig. 3 is the figure that the output with the jatropha curcas seed amount of output in the per unit cultivated area of cultivation Jatropha curcas and oil meal by oil that the oil expression of this seed is produced and oil expression compares.As shown in the drawing, with Jatropha curcas during as raw material, but the per unit cultivated area approximately year between 1.5 tons of produce oils, but simultaneously, can supervene 3.5 tons of oil expression oil meals between per unit cultivated area year, be equivalent to more than 2 times of oil production.
In a large amount of oil expression oil meals that produce, similarly contain with oil the Buddhist ripple ester that report has carcinogenic promoting agent like this in produce oil.So, can not be directly as the animal feed raw material, its purposes is limited to the lower fertilizer of added value and the lower solid fuel of unit price, is difficult to effectively apply flexibly the whole renewable resource that obtains by the cultivation Jatropha curcas.
In such situation, in present embodiment, the organic matter that contains Buddhist ripple ester by conduct uses the oil meal after the seed oil expression of Jatropha curcas, its Buddhist ripple ester can be decomposed and remove, and the oil meal of having removed Buddhist ripple ester can be improved its value as the animal feed raw material and release market again.Its result is significantly improved the industry rentability in the time of cultivating Jatropha curcas as a kind of industry, can provide oil more cheaply to market.In addition, can more effectively utilize reproducible living beings (biomass) resource of producing by as the growth of the Jatropha curcas of plant.
Then, with reference to Fig. 4, the seed of Jatropha curcas is shelled and the advantage when taking out the oil meal after oil expression after inner nucleus of the seed (kernel) and using as the organic matter that contains Buddhist ripple ester of the processing object in the organic manufacture method that contains high protein of present embodiment describes.Shown in this figure be, about the oil expression oil meal of jatropha curcas seed core with as the oil meal after the soybean extract oil of representational feedstuff, the result after just comparing as the important composition project of feedstuff.
Can learn from this figure, as the containing ratio of the protein of the most important composition of feedstuff, soybean is about 45% degree, and Jatropha curcas is more than 60%.About lipid containing ratio and ash content containing ratio, show: Jatropha curcas and soybean there is no too big difference, the fibre composition containing ratio, and Jatropha curcas is lacked than soybean.Hence one can see that, as long as can remove the Buddhist ripple ester as the carcinogenic promoting agent report, the oil meal after the jatropha curcas seed core oil expression of high protein and low fibre composition can become the feedstuff more excellent than soybean oil meal.
In addition, in the organic manufacture method that contains high protein of present embodiment, thereby make its fermentation decompose the object organic matter of Buddhist ripple ester after mixing the bacillus bacterium, be not limited to Jatropha curcas.The technological thought of present embodiment so long as contain the organic matter that contains high protein of Buddhist ripple ester, just can similarly be suitable for, and by decomposed its Buddhist ripple ester by the bacillus bacterium, can make aptly the organic matter that contains high protein.
the bacillus bacterium of using in the organic manufacture method that contains high protein as present embodiment, for example can use bacillus coagulans (Bacillus coagulans), Smith bacillus (Bacillus smithii), bacillus subtilis withered grass subspecies (Bacillus subtilis subsp.subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus cereus (Bacillus cereus), Bacillus natto (Bacillus subtilis var.natto) etc., but be not limited to these, can use the various bacillus bacterium in this genus.
Fig. 5 shows the result for the Buddhist ripple the rate of breaking-up ester in various bacterium comparative measurements organic matters.Experiment condition is as follows: after having mixed 1% thalline of the organic matter weight that contains Buddhist ripple ester, processed for 3 weeks at optimum culturing temperature (bacillus bacterium (Bacillus natto) and yeast are 37 ℃, are 30 ℃ to the aspergillus oryzae) bottom fermentation of various bacterium.Then, analyze by above-mentioned HPLC, in the organic matter between each bacterium before comparison process, how many % of contained Buddhist ripple ester amount are decomposed.The result demonstration, bacillus has the most excellent capacity of decomposition.
As above explanation, according to the organic matter that contains high protein of present embodiment and the manufacture method of feed, utilize and hold facile microorganism bacillus bacterium, simultaneously by simple operation, just can be from the organic matter that contains Buddhist ripple ester with the high de-agglomeration rate and remove at low cost Buddhist ripple ester as the report of carcinogenic promoting agent.
That is, decompose and remove in the processing procedure of Buddhist ripple ester, do not use expensive volatile reagent or can not produce the reluctant waste liquid that contains the toxicity material, and process in the situation that need not to import expensive control device or control machine.
In addition, by using at a low price and holding facile bacillus bacterium, make the organic matter that contains Buddhist ripple ester at relatively undemanding condition bottom fermentation, can decompose more well than the situation efficient of using other microorganism and remove Buddhist ripple ester.Its result, can with to process relevant first current cost, operating cost suppress ground lower the time the higher Buddhist ripple ester of realization remove rate.
Further, by the second order effect of bacillus bacterium, vitamin and mineral matter and other components in the organic matter after processing can be increased, when the organic matter after particularly processing is used in animal feed raw material etc., the nutrient in feed can also be improved.
In addition, the organic matter that contains Buddhist ripple ester by conduct uses the oil meal after the seed oil expression of Jatropha curcas, according to present embodiment, can decompose and remove the Buddhist ripple ester that surpasses as the accessory substance of the oil production carried out of cultivation Jatropha curcas in the oil expression oil meal that oil mass produces in large quantities, the oil expression oil meal that obtains can be improved as the animal feed raw material and be worth and put on market.
Further, as the organic matter that contains Buddhist ripple ester, by the oil meal after the nucleus of the seed oil expression of using the inside that the seed shelling of Jatropha curcas is taken out (jatropha curcas seed core oil expression oil meal), the concentration as the nutrient of feedstuff can be especially improved, the feedstuff more excellent than soybean oil meal can be made.Therefore, the oil expression oil meal after processing further can be improved value as the animal feed raw material, put on market.Its result, the rentability contribution of industry that can be to will cultivate Jatropha curcas as industry the time further improves, and can expect thus to make the effect that is stabilized in level more at a low price as the market price of the jatropha curcas oil of Renewable Energy Resources.
[the second embodiment]
Then, to the formation of the second embodiment of the present invention, describe with reference to Fig. 6 and Fig. 7.Fig. 6 is the figure of the operation of the manufacture method of the organic matter that contains high protein that shows present embodiment and feed.Fig. 7 is the figure of the variation of the Buddhist ripple ester content in the demonstration organic matter.
The organic matter that contains high protein of present embodiment and the manufacture method of feed, as shown in Figure 6, can make the manufacture method that comprises (A1) mixed processes, (A2) HTHP operation, (A3) agitating procedure, (A4) fermentation procedure.For other the point, can make the mode same with the first embodiment, even if in the present embodiment, as the organic matter that contains Buddhist ripple ester, also can use the oil meal after the seed oil expression of the Jatropha curcas (Jatropha curcas L.) of Euphorbiaceae or this seed shell and takes out oil meal after internal seeds core (kernel) extracts oil afterwards.
(A1) mixed processes
At first, mixing water in the organic matter that contains Buddhist ripple ester.At this moment, as mixed proportion, with respect to organic 4 mass parts that contain Buddhist ripple ester, preferably mix the water of 0.5～3 mass parts.Because set if so the mixed proportion of water, fermentation efficiency can improve.In addition, from this point of view, the mixed proportion that more preferably makes water is 2～3 mass parts.
(A2) HTHP operation
Then, the organic matter that contains Buddhist ripple ester and the mixed liquor of water are carried out autoclave sterilization.Thus, kill and to hinder the microorganism that utilizes the bacillus bacterium to ferment.Autoclave sterilization can carry out with general method with autoclave (autoclave).
Contain various microorganisms in normally used processing object organic matter, wherein, also have the situation that contains the microorganism that hinders the Buddhist ripple ester decomposition of being undertaken by the bacillus bacterium.Therefore, hinder microorganism in order to kill these, carry out autoclave sterilization.
(A3) agitating procedure
Then, the material that adds the bacillus bacterium in aqua sterilisa is added in mixed liquor after above-mentioned sterilization, be stirred well to the distribution that becomes homogeneous.At this moment, preferably with respect to aqua sterilisa 0.5～1 mass parts, add the bacillus bacterium of 0.004～0.2 mass parts.Because, set if so the mixed proportion of bacillus bacterium, can realize the fermentation of homogeneous.In addition, from this point of view, more preferably the mixed proportion with the bacillus bacterium is set as 0.04～0.12 mass parts.
(A4) fermentation procedure
Then, make interpolation add the aqua sterilisa of bacillus bacterium and the mixed liquor of stirring, make in confined conditions its fermentation.From the viewpoint of effectively fermenting, temperature conditions is preferably set to 30～50 ℃, more preferably is set as 37～50 ℃.In addition, as fermentation time, be preferably set to for 2～4 weeks.
After fermentation procedure finished, the mixture after the processing of taking out in fermenting cellar or the round became the organic matter that contains high protein that Buddhist ripple ester is decomposed under the effect of bacillus bacterium.In addition, by the second order effect of bacillus bacterium, make vitamin and mineral matter and other components in the mixture after processing increase.
At this, be that the reason in 2～4 weeks describes with reference to Fig. 7 to fermentation time.As shown in the drawing, in 1 week, the resolution ratio of Buddhist ripple ester is probably 50% after fermentation procedure begins, through being probably more than 80% after 2 weeks, through after 3 weeks be probably 95%, through being probably 99% after 4 weeks.Fermentation time more extends like this, and the resolution ratio of Buddhist ripple ester is just larger, if but the processing time prolongation, the cost that is used for the state of keeping can increase, and therefore requires the fermentation time of selecting resolution ratio and cost to average out.Carry out above such research, result as can be known, it is the most effective that fermentation time was set as for 2～4 weeks.
The organic manufacture method that contains high protein according to present embodiment, can decompose from the organic matter that contains Buddhist ripple ester with low cost and high throughput and remove Buddhist ripple ester, can produce the organic matter that contains high protein that is suitable for feed stripped and the feed that uses it.
In addition, can make practical bacillus bacterium process the organic fermentation time optimization of object, can remove in addition the principal element that hinders the Buddhist ripple ester decomposition of carrying out with the bacillus bacterium.Therefore, drop into minimum bacillus bacterium of limiting the quantity of, just can obtain the Buddhist ripple ester decomposition of maximum limit, can further to suppress ground lower with removing the necessary cost of Buddhist ripple ester in organic matter.
[the 3rd embodiment]
Then, with reference to Fig. 8, the formation of the 3rd embodiment of the present invention is described.This figure is the figure of operation that shows the manufacture method of the organic matter that contains high protein of present embodiment and feed.Be not use the bacillus bacterium itself, but on the kind bacterium use this point of the organic matter that contains high protein that will obtain in any embodiment of the present invention as fermentation use, present embodiment is different from the second embodiment.For other aspects, can make the mode identical with the second embodiment.
That is, (A1) mixed processes in the manufacture method of the organic matter that contains high protein of present embodiment and feed, (A2) HTHP operation can be made the operation identical with the second embodiment.
(A3) agitating procedure in the organic matter that contains high protein of present embodiment and the manufacture method of feed is different from the second embodiment, not to have added the material of bacillus bacterium to add in mixed liquor in aqua sterilisa, but the organic matter that contains high protein that will obtain by the manufacture method of present embodiment joins in aqua sterilisa, the material that obtains like this added in the mixed liquor of the sterilization that obtains by the HTHP operation and stirs.If like this, do not need again to prepare the bacillus bacterium at every turn, can reduce and make the necessary cost of organic matter that contains high protein.
At this moment, preferably with respect to aqua sterilisa 0.5～1 mass parts, add organic matter 0.02～1 mass parts that contains high protein.Because so set the organic mixed proportion that contains high protein, just can realize high fermentation efficiency with low cost.In addition, from such a viewpoint, the organic mixed proportion that more preferably will contain high protein is set as 0.2～0.4 mass parts.
In addition, (A4) fermentation procedure in the manufacture method of the organic matter that contains high protein of present embodiment and feed can be made the operation identical with the second embodiment.
According to the organic matter that contains high protein of present embodiment and the manufacture method of feed, there is no need each new bacillus bacterium of preparing.Therefore, can make decompose the necessary bacillus bacterium of Buddhist ripple ester total amount during than the second embodiment still less, result, the necessary cost that manufacturing can be contained the organic matter of high protein and feed suppress further low.
[the 4th embodiment]
Then, with reference to Fig. 9, the organic manufacture method that contains high protein of the 4th embodiment is described.This figure is the figure of operation that shows the manufacture method of the organic matter that contains high protein of present embodiment and feed.
Carry out in advance preculture from mix the bacillus bacterium the organic matter that contains Buddhist ripple ester, described cultured products is added to make its main fermentation in the organic matter that contains Buddhist ripple ester, contain on the organic matter this point of high protein thereby make, present embodiment is different from the second embodiment.For other aspects, can make the mode identical with the second embodiment.
＜preculture 〉
(B1) the first mixed processes
At first, mixing water in the organic matter that contains Buddhist ripple ester.At this moment, preferably with respect to organic matter 2 mass parts that contain Buddhist ripple ester, mixing water 0.5～1.5 mass parts.Because the mixed proportion of water is set for like this, the efficient of fermentation will improve.In addition, from this point of view, more preferably the mixed proportion with water is set as 1～1.5 mass parts.
(B2) the first HTHP operation
Then, with HTHP operation in the second embodiment in the same manner, will contain the organic matter of Buddhist ripple ester and the mixed liquor of water and carry out autoclave sterilization.
(B3) the first agitating procedure
Then, will stir in the mixed liquor that add after the aqua sterilisa that the bacillus bacterium is arranged adds above-mentioned sterilization to.At this moment, preferably with respect to aqua sterilisa 0.5 mass parts, add the bacillus bacterium of 0.002～0.1 mass parts.If because the mixed proportion of bacillus bacterium is set as like this, can realize the fermentation of homogeneous.In addition, from this point of view, more preferably the mixed proportion with the bacillus bacterium is set as 0.02～0.06 mass parts.
(B4) preculture operation
Then, the mixed liquor that obtains by the first agitating procedure is fermented in confined conditions.From the viewpoint of effectively fermenting, preferably 30～50 ℃ of temperature conditions are more preferably 37～50 ℃.In addition, the fermentation time favor speed is 1～7.
＜main fermentation 〉
(B5) the second mixed processes
Then, mixing water in the organic matter that contains Buddhist ripple ester.At this moment, preferably with respect to organic matter 5 mass parts that contain Buddhist ripple ester, mix the water of 2～4 mass parts.If because the mixed proportion of water is set as like this, the efficient of fermentation will improve.In addition, from this point of view, more preferably the mixed proportion with water is set as 3～4 mass parts.
(B6) the second HTHP operation
Then, with the first HTHP operation similarly, will contain the organic matter of Buddhist ripple ester and the mixed liquor of water and carry out autoclave sterilization.
(B7) the second agitating procedure
Then, will join in aqua sterilisa by the preculture product that preculture obtains.Then, will add the aqua sterilisa that this preculture product is arranged, and add in the mixed liquor by the second autoclave sterilization operation sterilization and also stir.
At this moment, preferably with respect to aqua sterilisa 1 mass parts, add preculture product 1～4 mass parts.If because the mixed proportion of preculture product is set as like this, can realize high fermentation efficiency with low cost.In addition, from this point of view, more preferably the mixed proportion with the preculture product is set as 2～4 mass parts.
(B8) main fermentation operation
Then, interpolation is contained the water of bacillus bacterium and the mixed liquor that stirs, make in confined conditions its fermentation.From the viewpoint of effective fermentation, temperature conditions is preferably set to 30～50 ℃, more preferably is set as 37～50 ℃.In addition, fermentation time preferably was set as for 2～4 weeks as described in the second implementation method.
After fermentation procedure finished, the mixture after the processing of taking out from fermenting cellar or round became the organic matter that contains high protein that the effect of Buddhist ripple ester by the bacillus bacterium is decomposed.In addition, by the second order effect of bacillus bacterium, the vitamin in the mixture after processing and mineral matter and other components increase.
As described above, according to the organic matter that contains high protein of present embodiment and the manufacture method of feed, can make bacillus bacterium Effective multiplication by preculture, the preculture product that obtains is added to making its main fermentation in the organic matter that contains Buddhist ripple ester, therefore can promote fermentation.
Thus, the Buddhist ripple ester in decomposing organic matter more effectively.
[the 5th embodiment]
Then, with reference to Figure 10, the organic manufacture method that contains high protein of the 5th embodiment is described.This figure is the figure of operation that shows the manufacture method of the organic matter that contains high protein of present embodiment and feed.
Present embodiment is, the bacillus bacterium is mixed into carries out in advance preculture in the organic matter that contains Buddhist ripple ester, the preculture product is added into carries out main fermentation in the organic matter that contains Buddhist ripple ester, thereby make the organic embodiment that contains high protein.Different from the 4th embodiment on the different this point of the mixed proportion that does not comprise its first and second mixed processes, thalline etc. or water.For other aspects, can be identical with the 4th embodiment.
＜preculture 〉
(C1) the first HTHP operation
(C2) the first agitating procedure
Then, in the organic matter that adds after the aqua sterilisa that the bacillus bacterium is arranged adds above-mentioned sterilization to, stir.At this moment, preferably with respect to aqua sterilisa 5～10 mass parts, add the bacillus bacterium of 0.01～0.6 mass parts.Because set if so the mixed proportion of bacillus bacterium, just can realize the fermentation of homogeneous.In addition, from this point of view, more preferably the mixed proportion with the bacillus bacterium is set as 0.1～0.6 mass parts.
(C3) preculture operation
Then, the mixed liquor that obtains by the first agitating procedure is fermented in confined conditions.From the viewpoint of effectively fermenting, temperature conditions is preferably set to 30～50 ℃, more preferably is set as 37～50 ℃.In addition, fermentation time is preferably set to 1～7 day.
＜main fermentation 〉
(C4) the second HTHP operation
(C5) the second agitating procedure
Then, will join in aqua sterilisa by the resulting preculture product of preculture.Then, will add the aqua sterilisa of this preculture product, and add in the organic matter by the second autoclave sterilization operation sterilization, and stir.
At this moment, preferably with respect to aqua sterilisa 50～100 mass parts, add the preculture product of 5～20 mass parts.Because so set the mixed proportion of preculture product, just can realize high fermentation efficiency with low cost.In addition, from this point of view, more preferably the mixed proportion with the preculture product is set as 10～20 mass parts.
(C6) main fermentation operation
Then, make and add the mixed liquor contain the water of bacillus bacterium and to stir and ferment in confined conditions.From the viewpoint of effective fermentation, temperature conditions is preferably set to 30～50 ℃, more preferably is set as 37～50 ℃.In addition, fermentation time was preferably set to for 2～4 weeks as described in the second embodiment.
After fermentation procedure finished, the mixture after the processing of taking out from fermenting cellar or round became the organic matter that contains high protein that the effect of Buddhist ripple ester by the bacillus bacterium is decomposed.In addition, by the second order effect of bacillus bacterium, the vitamin in the mixture after processing and mineral matter and other components increase.
In the organic matter that contains high protein of present embodiment and the manufacture method of feed, different from the 4th embodiment, be not that the organic matter that contains Buddhist ripple ester is added water, but the bacillus bacterium is added water.The water yield by so making the dissolving bacterium as much as possible, in the organic matter that has added the water that is dissolved with bacterium, bacterium is disperseed by homogeneous in a wider context, the fermentation of the organic matter integral body homogeneous more that also becomes.
Therefore, can expect that the fermentation efficiency of organic matter integral body uprises, the Buddhist ripple ester in more effective decomposing organic matter.
Embodiment
Below, will in the present invention about the relevant evaluation of serviceability of the embodiment of the manufacture method of the organic matter that contains high protein and feed and comparative example and the organic matter that contains high protein and feed by these acquisitions, describe with reference to Figure 11～Figure 14.Figure 11, Figure 12 are respectively figure, the charts of the variation of the Buddhist ripple ester content in the organic matter that shows in embodiment and comparative example.Figure 13 show to use the figure of feeding experiment result of chicken of feed of the present invention and the chart that shows its average weight transition.Figure 14 is figure and the chart that shows the feed intake of the chicken of using feed of the present invention.
(embodiment 1)
Before the operation of the manufacture method of the organic matter that contains high protein and feed, by following operation, extract oil from Jatropha curcas, obtain the Jatropha curcas residue.
At first, the seed of 24kg Jatropha curcas be separated into nucleus of the seed section and plant skin zone with peeling machine, only collecting nucleus of the seed section.The amount of collected nucleus of the seed section is approximately 14.4kg.Then, with pulverizer, nucleus of the seed section is ground into the size of diameter 2mm left and right.
Secondly, the nucleus of the seed section that will pulverize with electric oil press (model S100-200, the manufacturing of the サ of Co., Ltd. Application essence machine) extracts oil, and extracts oil, collects the nucleus of the seed oil meal (=Jatropha curcas residue) of discharging from oil press, makes it naturally cooling.The Jatropha curcas residue of collecting is about 7.2kg.
Then, by following operation, carry out preculture and main fermentation, obtain the organic matter that contains high protein that Buddhist ripple ester is decomposed.
At first, Jatropha curcas residue 10g was sterilized 15 minutes under 120 ℃ by high-temperature sterilizing chambers.
Then, as the bacillus bacterium, use bacillus coagulans (Bacillus coagulans, NBRC12583 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department), the aqua sterilisa 10g that has added this thalline 0.6g is added in the Jatropha curcas residue of sterilization, till the distribution that is stirred well to bacterium becomes homogeneous, afterwards 37 ℃ of lower precultures 3 days.
In addition, stirring is to keep flat down by the beaker of water that will add the mixed liquor that contains the bacillus bacterium, makes it rotate to carry out.Because use shovel to wait when stirring, the organic matter of solid shape is fragmentary is the mud shape, makes the gas permeability variation.For such stirring means, also identical in following embodiment and comparative example.
Then, Jatropha curcas residue 100g was sterilized 15 minutes under 120 ℃ by high-temperature sterilizing chambers, the aqua sterilisa 50g of nutrient solution (cultured products) 10g that will add preculture and obtain is added in the Jatropha curcas residue of sterilization, till the distribution that is stirred well to bacterium becomes homogeneous.
Afterwards, stirred when appending water 25g in every 8 days, 50 ℃ of lower main fermentations 24 days obtain the organic matter that contains high protein that the Buddhist ripple ester as this tunning the is decomposed Jatropha curcas of namely fermenting.
At last, this that adds 10 % by weight in test feed (WYNMOORE Chickbooster, Breeders Business Group society (Philippine) system) chicken contains the organic matter of high protein, the feed of manufacturing the present embodiment.
(embodiment 2)
Under the condition identical with embodiment 1, make the organic matter that contains high protein and the feed of the present embodiment.
(embodiment 3,4)
Except used Smith bacillus (Bacillus smithii, NBRC15311 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department) this point as the bacillus bacterium, other carries out similarly to Example 1, makes the organic matter that contains high protein and the feed of the present embodiment.
(embodiment 5,6)
Except used bacillus subtilis withered grass subspecies (Bacillus subtilis subsp.subtilis, NBRC13719 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department) this point as the bacillus bacterium, other carry out similarly to Example 1, make the organic matter that contains high protein and the feed of the present embodiment.
(embodiment 7,8)
Except used bacillus licheniformis (Bacillus licheniformis, NBRC12200 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department) this point as the bacillus bacterium, other carry out similarly to Example 1, make the organic matter that contains high protein and the feed of the present embodiment.
(embodiment 9)
Except used bacillus cereus (Bacillus cereus, NBRC15305 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department) this point as the bacillus bacterium, other carry out similarly to Example 1, make the organic matter that contains high protein and the feed of the present embodiment.
(embodiment 10,11)
Except used Bacillus natto (Bacillus subtilis var.natto, make institute (the Miyagi Prefecture celestial platform city wild district of city wall ginkgo raised path between farm fields 4-29) by the wild natto of limited commercial firm city wall obtain) this point as the bacillus bacterium, other carry out similarly to Example 1, make the organic matter that contains high protein and the feed of the present embodiment.
(comparative example 1)
At first, the Jatropha curcas residue 10g that obtains in embodiment 1 was sterilized 15 minutes under 120 ℃ by high-temperature sterilizing chambers.
Then, as the bacterium of adding in mixed liquor, use Lactobacillus delbrueckii subsp (Lactobacillus delbrueckii subsp.delbrueckii, NBRC3202 strain, obtained by independent administrative legal person's products assessment technique basal disc organization's Biological Genetic Resources department), to add the aqua sterilisa 10g that its thalline 0.6g is arranged to add in the mixed liquor of sterilization, till being stirred well to the distribution that becomes homogeneous, afterwards in 30 ℃ of lower precultures 3 days.
Then, Jatropha curcas residue 100g was sterilized 15 minutes under 120 ℃ by high-temperature sterilizing chambers, to add the aqua sterilisa 50g of nutrient solution (cultured products) 10g that preculture is arranged and obtain to add in the Jatropha curcas residue of sterilization, till the distribution that is stirred well to bacterium becomes homogeneous.
Afterwards, 30 ℃ of lower main fermentations 24 days, as this tunning, obtain the organic matter fermentation Jatropha curcas that contains high protein that is decomposed as Buddhist ripple ester.
In addition, not carrying out in comparative example 1 adding water and stirring in every 8 days, is 30 ℃ but make fermentation temperature.This is because the bacterial classification of comparative example 1 is suitable at such condition bottom fermentation.
At last, this organic matter that contains high protein is added 10 % by weight to chicken with test feed (WYNMOORE Chickbooster, Breeders Business Group society (Philippine) system), make the feed of this comparative example.
(evaluation)
＜1. the resolution ratio of Buddhist ripple ester 〉
To the organic Buddhist ripple ester content (PE content) that contains high protein in embodiment and comparative example, from the beginning day of main fermentation until till the 24th day, measured in every 8 days, calculate the average resolution ratio of Buddhist ripple ester.Its result represents in Figure 11 and Figure 12.
At this, in each embodiment and comparative example, as the organic matter that contains Buddhist ripple ester, all use the nucleus of the seed (kernel) of the inside of taking out after the seed shelling with the Jatropha curcas (Jatropha curcas L.) of Euphorbiaceae.In addition, " bacterial classification " in Figure 11 is expressed as the Buddhist ripple ester in decomposing organic matter and the kind name of the bacterium of using." bacterial strain " shows that in embodiment 10,11 it is obtained by the wild natto manufacturing of limited commercial firm city wall institute (the Miyagi Prefecture celestial platform city wild district of city wall ginkgo raised path between farm fields 4-29), for other embodiment, demonstration is obtained by NBRC (NITE Biological Resource Center, Japanese technology assessment research institute's living resources center), and shows its strain number.Begin to show in every 8 days the content of the Buddhist ripple ester in mixture fermentation procedure in " PE content (mg/g) " from main fermentation.
(1) assay method of PE content
The following mensuration of content of the Buddhist ripple ester in fermentation procedure in mixture.
At first, add carrene (Dichloromethane) 20ml in becoming the sample 1.5g of determination object, pulverized 2 minutes by homogenizer, separate in centrifuge, collect residue.Add carrene (Dichloromethane) 20ml, concuss 1 minute, centrifugal rear filtration in centrifuge to this residue.Repeat 5 times above-mentioned operation, collect whole filtered fluids, flow down drying at drying nitrogen.
Then, this thing is dissolved in the 5ml oxolane, with the filter filtration of 0.2 μ m, 20 μ l is injected HPLC (high performance liquid chromatography).HPLC uses WATERS (registration mark) 2690 (water generation company (Waters Corporation) systems), detector use WATERS996 (water generation company system).HPLC carries out under the following conditions.
HPLC chromatographic column: symmetry C18,3.5 μ m, 100 * 4.6mm
Solvent: A contains 1L distilled water, B acetonitrile (HPLC level), the C oxolane (HPLC level) of 1.75ml phosphoric acid (o-phosphoric acid) (85%)
Flow: 1ml/ minute
Operation: (1) A liquid 60%, B liquid 40% (15 minutes), (2) A liquid 25%, (10 minutes), (4) C liquid 100% (10 minutes) till B liquid 75% (15 minutes), till (3) B liquid 100%
(2) resolution ratio of Buddhist ripple ester
As shown in figure 11, the PE content of embodiment 1-11 begins day to be 2.98mg/g in main fermentation, but was respectively average 1.17mg/g, 0.25mg/g, 0.14mg/g at the 8th day, the 16th day, the 24th day.
The resolution ratio of the Buddhist ripple ester of therefore, the 8th day, the 16th day, the 24th day is respectively 60.8%, 91.7%, 95.4%.
On the other hand, the PE content of comparative example 1 begins day to be 2.98mg/g in main fermentation, but was respectively 2.06mg/g, 1.78mg/g, 1.66mg/g at the 8th day, the 16th day, the 24th day.
The Buddhist ripple the rate of breaking-up ester of therefore, the 8th day, the 16th day, the 24th day is respectively 30.9%, 40.3%, 44.3%.
Result as can be known, acts on the bacterium of decomposing Buddhist ripple ester from the organic matter that contains Buddhist ripple ester by the bacillus bacterium is used thus, can effectively decompose Buddhist ripple ester.
On the other hand, when other bacterium Lactobacillus delbrueckii subsps being used the bacterium that acts on decomposition Buddhist ripple ester from the organic matter that contains Buddhist ripple ester, can't remove fully Buddhist ripple ester.
＜2. the feeding experiment of chicken 〉
The feed of use by obtaining in embodiment and comparative example carries out the growth test of chick by the following method at Palawan Agribusiness Development Foundation Inc. (Philippine).
The chick of the 4th day after hatching by one group 3, is grouped into cage A, and B in C, gives respectively the feed that obtains in embodiment, is confirmed whether the test of growing up fully.
In cage A, give to add the organic matter that contains high protein (processing meal) 10% that is obtained by the Smith bacillus decomposition Buddhist ripple ester of embodiment 3 and the feed that obtains chicken in test feed (WYNMOORE Chickbooster, Breeders Business Group society (Philippine) system).In addition, in cage B, give to add in test feed the chicken identical with cage A that 10% bacillus subtilis withered grass subspecies by embodiment 6 are decomposed Buddhist ripple ester and the organic matter that contains high protein that obtains and the feed that obtains, in cage C, give to add in test feed the chicken identical with cage A that 10% the Bacillus natto by embodiment 11 is decomposed Buddhist ripple ester and the organic matter that contains high protein that obtains and the feed that obtains.
Then, raise till hatched rear 14 days, when beginning and every body weight of measuring each chicken on the 7th.Whichsoever cage all constantly gives feed till the off-test, and drinking water is free drinking-water.
The chick of using is to be derived from broiler chicken (broiler) to plant 9 female chick that do not inoculate vaccine that the kind egg of chicken hatches, and should raise 4 in advance by female chick.The changes of weight that shows each chicken at Figure 13 shows the feed intake at Figure 14.
As shown in figure 13, can learn, whichsoever the chicken of cage all grows up smoothly.In addition, as shown in figure 14, no matter at which cage, all absorb rightly feed.
Therefore as can be known, according to the manufacture method that contains organic matter and the feed of high protein of the present invention, use the Buddhist ripple ester in bacillus bacterium decomposing organic matter and the organic matter that contains high protein that obtains, can be suitable to feed.
The present invention is not limited to above embodiment and embodiment, need not put into words, and can implement various changes within the scope of the invention.
For example, although use in the above-described embodiments Jatropha curcas, also can be suitable for the present invention in other organic matter that contains Buddhist ripple ester.In addition, although above-mentioned evaluation is carried out chick, also the feed of the organic matter that contains high protein by manufacturing of the present invention as pig or ox, horse, other domestic animal can be used.
Utilizability on industry
The present invention can suitablely be used in the feed of the domestic animal of making chicken etc.
Claims (11)
1. an organic manufacture method that contains high protein, is characterized in that, the organic matter that will contain Buddhist ripple ester belongs to bacterium with bacillus (Bacillus) and mixes and make it fermentation, and the Buddhist ripple ester in described organic matter is decomposed.
2. the organic manufacture method that contains high protein according to claim 1, it is characterized in that, with respect to organic matter 4 mass parts that contain Buddhist ripple ester, mixing water 0.5～3 mass parts, after autoclave sterilization, be added on and add the material that obtains after Bacillus bacterium 0.004～0.2 mass parts in aqua sterilisa 0.5～1 mass parts, in 30～50 ℃ of 2～4 weeks of bottom fermentation.
3. organic manufacture method that contains high protein, it is characterized in that, with respect to organic matter 4 mass parts that contain Buddhist ripple ester, mixing water 0.5～3 mass parts, after autoclave sterilization, be added on and add the material that obtains after the organic matter that contains high protein 0.02～1 mass parts that obtains by the described manufacture method of right claims 1 or 2 in water 0.5～1 mass parts, in 30～50 ℃ of 2～4 weeks of bottom fermentation.
4. the organic manufacture method that contains high protein according to claim 1, it is characterized in that, the organic matter that will contain Buddhist ripple ester with carry out preculture after the Bacillus bacterium mixes, then, the organic matter that will contain Buddhist ripple ester mixes with the Bacillus bacterium, add the preculture product in this mixture, make its main fermentation, the Buddhist ripple ester in described organic matter is decomposed.
5. the organic manufacture method that contains high protein according to claim 4, it is characterized in that, organic matter 2 mass parts that will contain Buddhist ripple ester are mixed with water 0.5～1.5 mass parts, after autoclave sterilization, be added on and added the material that obtains after Bacillus bacterium 0.002～0.1 mass parts in aqua sterilisa 0.5 mass parts, in 30～50 ℃ of lower precultures 1～7 day
Organic matter 5 mass parts that will contain Buddhist ripple ester are mixed with water 2～4 mass parts, after autoclave sterilization, be added on the material that obtains after preculture product 1～4 mass parts that adds in aqua sterilisa 1 mass parts by the preculture gained, in 30～50 ℃ of lower 2～4 weeks of main fermentation.
6. the organic manufacture method that contains high protein according to claim 4, it is characterized in that, after organic matter 10 mass parts that will contain Buddhist ripple ester are carried out autoclave sterilization, be added on and add the material that obtains after Bacillus bacterium 0.01～0.6 mass parts in aqua sterilisa 5～10 mass parts, in 30～50 ℃ of lower precultures 1～7 day
After organic matter 100 mass parts that will contain Buddhist ripple ester are carried out autoclave sterilization, be added on the material that obtains after preculture product 5～20 mass parts that add in aqua sterilisa 50～100 mass parts by the preculture gained, in 30～50 ℃ of lower 2～4 weeks of main fermentation.
7. the described organic manufacture method that contains high protein of any one according to claim 1～6, it is characterized in that, as the described organic matter that contains Buddhist ripple ester, the oil meal of use after with the oil expression of the seed of the Jatropha curcas (JatrophacurcasL.) of Euphorbiaceae perhaps uses the oil meal after the nucleus of the seed oil expression of the inside that its seed shelling is taken out.
8. an organic matter that contains high protein, is characterized in that, the organic matter that will contain Buddhist ripple ester belongs to bacillus (Bacillus) makes its fermentation after bacterium mixes, and makes the Buddhist ripple ester in described organic matter decompose and obtain.
9. the organic matter that contains high protein according to claim 8, it is characterized in that, as the described organic matter that contains Buddhist ripple ester, the oil meal of use after with the oil expression of the seed of the Jatropha curcas (JatrophacurcasL.) of Euphorbiaceae perhaps uses the oil meal after the nucleus of the seed oil expression of the inside that its seed shelling is taken out.
10. the manufacture method of a feed, is characterized in that, the organic matter that will contain Buddhist ripple ester belongs to bacillus (Bacillus) makes its fermentation after bacterium mixes, and the Buddhist ripple ester in described organic matter is decomposed.
11. a feed is characterized in that, contain will contain the organic matter of Buddhist ripple ester belong to bacillus (Bacillus) make its fermentation after bacterium mixes, with the resulting tunning of described Buddhist ripple ester decomposition.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010181021A JP5424268B2 (en) | 2010-08-12 | 2010-08-12 | Method for producing high protein content organic matter, high protein content organic matter, feed production method, and feed |
| JP2010-181021 | 2010-08-12 | ||
| PCT/JP2011/004558 WO2012020573A1 (en) | 2010-08-12 | 2011-08-11 | Manufacturing method for high-protein-containing organic product, high-protein-containing organic product, manufacturing method for animal feed, and animal feed |
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| CN103153083A true CN103153083A (en) | 2013-06-12 |
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| CN2011800483660A Pending CN103153083A (en) | 2010-08-12 | 2011-08-11 | Method for producing high-protein-content organic material, method for producing feed, and feed |
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| Country | Link |
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| JP (1) | JP5424268B2 (en) |
| CN (1) | CN103153083A (en) |
| WO (1) | WO2012020573A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110241045A (en) * | 2019-06-25 | 2019-09-17 | 四川农业大学 | A kind of bacillus megaterium and its application in the fermentation detoxification of the leprosy seedcake dregs of rice |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104082525B (en) * | 2014-06-19 | 2016-05-04 | 四川大学 | The application of strange land Klebsiella in barbadosnut pulp reduce toxicity of dwelling |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101427730A (en) * | 2008-10-14 | 2009-05-13 | 上海三瑞高分子材料有限公司 | Method for abstracting and removing phorbol ester from barbadosnut seed oil meal |
| CN102316998A (en) * | 2009-02-13 | 2012-01-11 | 出光兴产株式会社 | Method for removing phorbol ester from organic material, method for producing high-protein-content organic material, method for producing feed, and feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2774899B2 (en) * | 1992-03-25 | 1998-07-09 | 京都府 | Production method of feed for cultured fish |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101427730A (en) * | 2008-10-14 | 2009-05-13 | 上海三瑞高分子材料有限公司 | Method for abstracting and removing phorbol ester from barbadosnut seed oil meal |
| CN102316998A (en) * | 2009-02-13 | 2012-01-11 | 出光兴产株式会社 | Method for removing phorbol ester from organic material, method for producing high-protein-content organic material, method for producing feed, and feed |
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| Title |
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| 张春强等: "麻疯树籽实的毒性原理及其脱毒处理方法", 《饲料博览》, no. 5, 31 December 2009 (2009-12-31), pages 29 - 31 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241045A (en) * | 2019-06-25 | 2019-09-17 | 四川农业大学 | A kind of bacillus megaterium and its application in the fermentation detoxification of the leprosy seedcake dregs of rice |
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| Publication number | Publication date |
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| WO2012020573A1 (en) | 2012-02-16 |
| JP2012039884A (en) | 2012-03-01 |
| JP5424268B2 (en) | 2014-02-26 |
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