CN103148823B - Device for measuring thicknesses of aerobic layer and anaerobic layer of biological film and measuring method - Google Patents
Device for measuring thicknesses of aerobic layer and anaerobic layer of biological film and measuring method Download PDFInfo
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- CN103148823B CN103148823B CN201310081510.1A CN201310081510A CN103148823B CN 103148823 B CN103148823 B CN 103148823B CN 201310081510 A CN201310081510 A CN 201310081510A CN 103148823 B CN103148823 B CN 103148823B
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- 238000000034 method Methods 0.000 title claims abstract description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 158
- 239000001301 oxygen Substances 0.000 claims abstract description 158
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 158
- 239000002351 wastewater Substances 0.000 claims abstract description 67
- 239000012528 membrane Substances 0.000 claims description 137
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 47
- 238000003760 magnetic stirring Methods 0.000 claims description 25
- 229910052757 nitrogen Inorganic materials 0.000 claims description 23
- 239000007789 gas Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000005273 aeration Methods 0.000 claims description 15
- 230000002572 peristaltic effect Effects 0.000 claims description 13
- 230000000630 rising effect Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 239000004677 Nylon Substances 0.000 claims description 7
- 229920001778 nylon Polymers 0.000 claims description 7
- 230000001737 promoting effect Effects 0.000 claims description 3
- 229910001873 dinitrogen Inorganic materials 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 10
- 229910052698 phosphorus Inorganic materials 0.000 description 10
- 239000011574 phosphorus Substances 0.000 description 10
- 230000001105 regulatory effect Effects 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 238000009792 diffusion process Methods 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 230000001651 autotrophic effect Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 235000008935 nutritious Nutrition 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910003208 (NH4)6Mo7O24·4H2O Inorganic materials 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a device for measuring thicknesses of an aerobic layer and an anaerobic layer of a biological film and a measuring method. The method comprises the steps that a wide-mouth bottle for containing waste water and a biological film testing tank are adopted; oxygen is charged into the wide-mouth bottle, so as to adjust the dissolved oxygen concentration of the waste water; after the dissolved oxygen concentration of the waste water in the wide-mouth bottle reaches a set value, the waste water is led into the biological film testing tank; after the waste water concentration in the wide-mouth bottle and the biological film testing tank reaches an experimental value, the biological film is put into the biological film testing tank; the concentration distribution of micro dissolved oxygen inside the biological film is measured through a dissolved oxygen microelectrode; and the thicknesses of the aerobic layer and the anaerobic layer of the biological film are calculated.
Description
Technical field
The present invention relates to electrochemical analysis field, particularly relate to the device and assay method that measure biological membrane aerobic layer thickness and anaerobic layer thickness.
Background technology
In biomembrance process waste water treatment process, biological membrane wastewater treatment layer is made up of aerobic layer and anaerobic layer.Biological denitrificaion process is carried out to waste water, no matter is traditional nitration denitrification biological denitrification process or Process of Biological Nitrogen Removal (as SHARON technique, ANAMMOX technique etc.), all can has aerobic and anaerobic processes.The oxidation of ammonia, mainly occurs in biological membrane aerobic layer, and environment solubilize oxygen concentration and hydraulics one timing, oxygen is larger at biological membrane inner transmission matter resistance, and aerobic layer thickness is less, is more difficult to efficiently carry out for ammoxidation process.Anaerobic denitrifying process mainly occurs in biological membrane anaerobic layer, and environment solubilize oxygen concentration and hydraulics one timing, oxygen is less at biological membrane inner transmission matter resistance, and anaerobic layer thickness is less, is also more difficult to efficiently carry out for anaerobic denitrifying process.Therefore, in biomembrance process wastewater denitrification system, suitable biological membrane aerobic layer thickness and anaerobic layer thickness, the efficient removal for total nitrogen has very important impact.But, in prior art not to how effectively, fast, easily to the technology that the carrying out of biological membrane aerobic layer thickness and anaerobic layer thickness is measured.
Summary of the invention
For above-mentioned technical matters, the invention provides and a kind of measure the inner dissolved oxygen concentration distribution of biological membrane, thus effectively, easy, measure biological membrane aerobic layer thickness and the mensuration biological membrane aerobic layer thickness of anaerobic layer thickness and the device of anaerobic layer thickness and assay method quickly and accurately.
To achieve these goals, the technical solution used in the present invention is as follows:
A kind of device measuring biological membrane aerobic layer thickness and anaerobic layer thickness, it is characterized in that: comprise oxygen conveying device, first container of waste water is housed and the biological membrane test trough of waste water is housed, described first container is provided with draft tube, rising pipe and venthole, under the draft tube of this first container and the lower end of rising pipe are all positioned at the waste water liquid level of this first container, the outlet side of described oxygen conveying device is connected with the draft tube of described first container, also comprise peristaltic pump, the rising pipe of described first container is connected with the water inlet end of peristaltic pump, the water side of this peristaltic pump is communicated by the inside of water pipe with described biological membrane test trough, this biological membrane test trough is also provided with return pipe and communicates with described first container,
Also comprise block rubber, this block rubber is arranged on bottom described biological membrane test trough, waste water liquid level in described biological membrane test trough, higher than the end face of described block rubber, the end face of this block rubber is also provided with for the biomembranous nylon wire of fixed placement at block rubber end face;
Also comprise dissolved oxygen micro electrode system and portable dissolved oxygen instrument, described dissolved oxygen micro electrode system comprises dissolved oxygen micro electrode, three-dimensional micro-electrode thruster and dissolved oxygen DO Test Host, the biofilm contact of described dissolved oxygen micro electrode by the perforate at described biological membrane test trough top and the end face of described block rubber are arranged, described three-dimensional micro-electrode thruster is for promoting described dissolved oxygen micro electrode stepping, described dissolved oxygen DO Test Host is used for calculating the data that described dissolved oxygen micro electrode receives, process and display, described portable dissolved oxygen instrument is arranged in described biological membrane test trough, and the bottom of this portable dissolved oxygen instrument is arranged in the waste water of described biological membrane test trough.
Described oxygen conveying device comprises an oxygen bottle, nitrogen cylinder, two flowmeters and a gas mixer chamber, described oxygen bottle is connected the input end of a flowmeter respectively with the output terminal of nitrogen cylinder, the output terminal of these two flowmeters is connected with two air intake openings of described gas mixer chamber successively, and the gas outlet of described gas mixer chamber is connected with the draft tube of described first container.
Described first container is the wide-necked bottle of band plug, the volume of this wide-necked bottle is 5L, plug on described wide-necked bottle arranges three through holes, wherein first through hole is installed with the draft tube of described first container, second through hole is installed with the rising pipe of described first container, and the 3rd through hole is used for wide-necked bottle inner chamber to be communicated with ambient atmosphere.
Described portable dissolved oxygen instrument is cylindrical, and this portable dissolved oxygen instrument cover has rubber plug, and the top of described biological membrane test trough is provided with and coordinates with described rubber plug, for being arranged in the mounting hole in described biological membrane test trough by described portable dissolved oxygen instrument.
Be provided with the aeration tube dish being arranged in waste water in described wide-necked bottle, the air intake opening of this aeration tube dish is connected with the end of the draft tube of described first container, and the below of described aeration tube dish is provided with the first magnetic stirring apparatus.
The bottom of described biological membrane test trough is provided with the second magnetic stirring apparatus and the 3rd magnetic stirring apparatus, and this second magnetic stirring apparatus and the 3rd magnetic stirring apparatus are positioned at the both sides of described block rubber.
Measure a method for biological membrane aerobic layer thickness and anaerobic layer thickness, use the device of said determination biological membrane aerobic layer thickness and anaerobic layer thickness, it is characterized in that, carry out in accordance with the following steps:
S1: artificial distribution, use ammonium bicarbonate, dipotassium hydrogen phosphate and sodium bicarbonate configuration waste water, the pH value regulating waste water is 8.0, and the waste water 4L loading volume getting configuration is in the described wide-necked bottle of 5L;
S2: the gas in oxygen bottle and nitrogen cylinder drawn and passes into after mixing in the waste water in wide-necked bottle, opening aeration tube dish and carry out aeration, open the first magnetic stirring apparatus and stir, regulating the oxygen concentration of the waste water solution in wide-necked bottle to 2.5mg/L;
S3: the waste water in wide-necked bottle is caused biological membrane test trough by peristaltic pump with plastic tube, after waste water is full of biological membrane test trough, draws the waste water in biological membrane test trough and loops back in wide-necked bottle;
Open the second magnetic stirring apparatus bottom biological membrane test trough and the 3rd magnetic stirring apparatus, waste water in biological membrane test trough is stirred, the portable dissolved oxygen instrument of band plug is inserted and secured in biological membrane test trough, oxyty in test organisms film test trough, until in biological membrane test trough, waste water oxyty is consistent with waste water oxyty in wide-necked bottle, and be stabilized in 2.5 mg/L;
S4: choose fritter biological membrane and put into test trough from one-step autotrophic nitrogen removal system, and be fixed on the upper surface of block rubber with the nylon wire of macropore, dissolved oxygen micro electrode is inserted biological membrane test trough, with the inner microcell dissolved oxygen distribution of test organisms film.
In step S1, the water distribution water quality of the waste water configured is as following table:
| Ammonia nitrogen (mg/L) | TP（mg/L） | Microelement nutritious liquid (ml/L) | PH value | DO（mg/L） |
| 200 | 5 | 2 | 8.0 | 2.5 |
Wherein shown in microelement nutritious liquid table composed as follows:
| Trace element | Concentration (g/L) |
| EDTA | 5.0 |
| CoCl 2·6H 2O | 1.6 |
| ZnSO 4·7H 2O | 2.2 |
| MnCl 2·4H 2O | 5.1 |
| CuSO 4·5H 2O | 1.6 |
| (NH 4) 6Mo 7O 24·4H 2O | 1.1 |
| CaCl 2·2H 2O | 5.5 |
| FeSO 4·7H 2O | 5.0 |
Wherein DO refers to dissolved oxygen DO, i.e. the milligram number of oxygen in every premium on currency.TP(total phosphorus, total phosphorus), the phosphorus-containing compound in water is mainly divided three classes: orthophosphate, condensed phosphate and organophosphorus; The phosphorus of form various in water is converted into orthophosphate by clearing up, and the data recorded are total phosphorus.
In described step S4, the step of the inner microcell dissolved oxygen distribution of test organisms film is: by the most advanced and sophisticated locus of three-dimensional micro-electrode propeller control dissolved oxygen micro electrode, make the tip of dissolved oxygen micro electrode slowly close to biological membrane, when the tip of dissolved oxygen micro electrode arrives biofilm surface, the reading of record dissolved oxygen DO Test Host display, after this, dissolved oxygen micro electrode is at every turn perpendicular to biological membrane direction stepping 20 μm, dissolved oxygen DO Test Host calculates biological membrane different-thickness place dissolved oxygen concentration, by that analogy, the inner dissolved oxygen concentration distribution of test organisms film, calculate biological membrane aerobic layer thickness and anaerobic layer thickness.
The computing method of described biological membrane different-thickness place dissolved oxygen concentration are: according to law of conservation of mass, under lower state:
The amount that the amount that amount=diffusion rate of dissolved oxygen that diffusion rate of dissolved oxygen enters goes out+dissolved oxygen DO reaction consumes
The amount diffused into is:
The amount diffused out is:
The amount that reaction consumes:
Then have:
Arrange:
Wherein
cfor oxyty, unit is g/m
3;
dfor dissolved oxygen DO is at biological membrane intraparticle diffusivity, unit is m
2/ s; OUR is the wear rate of dissolved oxygen DO, and unit is g/ (m
3s);
zfor biofilm thickness, unit is m.
Good effect of the present invention is:
The present invention measures the distribution of biological membrane interior microscopic dissolved oxygen concentration by dissolved oxygen micro electrode, calculates biological membrane aerobic layer thickness and anaerobic layer thickness.
Load after configuring waste water in wide-necked bottle, open oxygen bottle and nitrogen cylinder, pass in wide-necked bottle after the gas and vapor permeation in oxygen bottle and nitrogen cylinder and aeration is carried out to waste water, open the first magnetic stirring apparatus simultaneously.After the waste water in wide-necked bottle reaches certain oxyty, open peristaltic pump by the waste water suction biological membrane test trough in wide-necked bottle, unlatching simultaneously second and the 3rd magnetic stirring apparatus, after the waste water in this biological membrane test trough is full, the waste water in this biological membrane test trough is led back in wide-necked bottle by return pipe.
Get the biological membrane of one-step autotrophic nitrogen removal system, biological membrane is placed on the block rubber upper surface in biological membrane test trough, and is fixed on block rubber with macropore nylon wire.The effect of nitrogen cylinder is the oxyty regulating waste water, keeps the oxyty in the waste water in wide-necked bottle and biological membrane test trough to be all stabilized in 2.5 mg/L.
Dissolved oxygen microelectrode is carefully inserted biological membrane test trough, by the most advanced and sophisticated locus of three-dimensional micro-electrode propeller control oxygen microelectrode, make dissolved oxygen microelectrode most advanced and sophisticated slowly close to biological membrane, when microelectrode tip arrives biofilm surface, the reading of record dissolved oxygen microelectrode main frame display, after this each perpendicular to biological membrane direction stepping 20 μm, and record biological membrane different-thickness place dissolved oxygen concentration, by that analogy, the inner dissolved oxygen concentration space distribution of difference place, test organisms film surface biological membrane, thus determine oxygen layer thickness and anaerobic layer thickness.
Accompanying drawing explanation
Fig. 1 is that the principle of work that the present invention measures the device of biological membrane aerobic layer thickness and anaerobic layer thickness carrys out schematic diagram;
Fig. 2 is the schematic diagram of gas mixer chamber;
Fig. 3 is the inner dissolved oxygen concentration distribution in biological membrane first position, matched curve and second derivative figure;
Fig. 4 is the inner dissolved oxygen concentration distribution in biological membrane second position, matched curve and second derivative figure;
Fig. 5 is the inner dissolved oxygen concentration distribution in biological membrane the 3rd position, matched curve and second derivative figure.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
As shown in Figure 1, a kind of device measuring biological membrane aerobic layer thickness and anaerobic layer thickness, comprise oxygen conveying device, first container 1 of waste water is housed and the biological membrane test trough 2 of waste water is housed, described first container 1 is provided with draft tube, rising pipe and venthole, under the draft tube of this first container 1 and the lower end of rising pipe are all positioned at the waste water liquid level of this first container 1, the outlet side of described oxygen conveying device is connected with the draft tube of described first container 1, also comprise peristaltic pump 3, the rising pipe of described first container 1 is connected with the water inlet end of peristaltic pump 3, the water side of this peristaltic pump 3 is communicated by the inside of water pipe with described biological membrane test trough 2, this biological membrane test trough 2 is also provided with return pipe and communicates with described first container 1,
Also comprise block rubber 4, this block rubber 4 is arranged on bottom described biological membrane test trough 2, waste water liquid level in described biological membrane test trough 2, higher than the end face of described block rubber 4, the end face of this block rubber 4 is also provided with for the biomembranous nylon wire of fixed placement at block rubber end face;
Also comprise dissolved oxygen micro electrode system and portable dissolved oxygen instrument 6, described dissolved oxygen micro electrode system comprises dissolved oxygen micro electrode 5, three-dimensional micro-electrode thruster and dissolved oxygen DO Test Host, the biofilm contact of described dissolved oxygen micro electrode 5 by the perforate at described biological membrane test trough 2 top and the end face of described block rubber 4 are arranged, described three-dimensional micro-electrode thruster is for promoting the stepping of described dissolved oxygen micro electrode 5, described dissolved oxygen DO Test Host is used for calculating the data that described dissolved oxygen micro electrode 5 receives, process and display, described portable dissolved oxygen instrument 6 is arranged in described biological membrane test trough 2, and the bottom of this portable dissolved oxygen instrument 6 is arranged in the waste water of described biological membrane test trough 2.
Described oxygen conveying device comprises an oxygen bottle, nitrogen cylinder, two flowmeters 11 and a gas mixer chamber 12, described oxygen bottle is connected the input end of a flowmeter 11 respectively with the output terminal of nitrogen cylinder, the output terminal of these two flowmeters 11 is connected with two air intake openings of described gas mixer chamber 12 successively, and the gas outlet of described gas mixer chamber 12 is connected with the draft tube of described first container 1.
Described first container 1 is the wide-necked bottle of band plug, and the volume of this wide-necked bottle is 5L, and the plug on described wide-necked bottle arranges three through holes, and wherein first through hole is installed with the draft tube of described first container 1, and second through hole is installed with the rising pipe of described first container 1; 3rd through hole is used for wide-necked bottle inner chamber to be communicated with ambient atmosphere, and object is to discharge nitrogen and oxygen mixed gas.
Described portable dissolved oxygen instrument 6 is cylindrical, and this portable dissolved oxygen instrument 6 cover has rubber plug, and the top of described biological membrane test trough 2 is provided with and coordinates with described rubber plug, for being arranged in the mounting hole in described biological membrane test trough 2 by described portable dissolved oxygen instrument 6.
Be provided with the aeration tube dish 7 being arranged in waste water in described wide-necked bottle, the air intake opening of this aeration tube dish 7 is connected with the end of the draft tube of described first container 1, and the below of described aeration tube dish 7 is provided with the first magnetic stirring apparatus 8.
The bottom of described biological membrane test trough 2 is provided with the second magnetic stirring apparatus 9 and the 3rd magnetic stirring apparatus 10, and this second magnetic stirring apparatus 9 and the 3rd magnetic stirring apparatus 10 are positioned at the both sides of described block rubber 4.
The present invention measures the distribution of biological membrane interior microscopic dissolved oxygen concentration by dissolved oxygen micro electrode, calculates biological membrane aerobic layer thickness and anaerobic layer thickness.
Load after configuring waste water in wide-necked bottle, open oxygen bottle and nitrogen cylinder, the flowmeter range controlling oxygen flow is 10-100 ml/min, and the flowmeter range controlling nitrogen flow is 5-45 L/min; Pass in wide-necked bottle after gas and vapor permeation in oxygen bottle and nitrogen cylinder and aeration is carried out to waste water, open the first magnetic stirring apparatus simultaneously.After the waste water in wide-necked bottle reaches certain oxyty, open peristaltic pump by the waste water suction biological membrane test trough 2 in wide-necked bottle, unlatching simultaneously second and the 3rd magnetic stirring apparatus 10, after the waste water in this biological membrane test trough 2 is full, the waste water in this biological membrane test trough 2 is led back in wide-necked bottle by return pipe.
Get the biological membrane of one-step autotrophic nitrogen removal system, biological membrane is placed on block rubber 4 upper surface in biological membrane test trough 2, and is fixed on block rubber with macropore nylon wire.The effect of nitrogen cylinder is the oxyty regulating waste water, is read the oxyty in waste water by portable dissolved oxygen instrument 6, by regulating the ratio of oxygen and nitrogen in mixed gas, can control waste water oxyty, and oxyty can be made to be stabilized in certain concentration.The oxyty in the waste water in wide-necked bottle and biological membrane test trough 2 is kept all to be stabilized in 2.5 mg/L in the present invention.
Dissolved oxygen microelectrode is carefully inserted biological membrane test trough 2, by the most advanced and sophisticated locus of three-dimensional micro-electrode propeller control oxygen microelectrode, make dissolved oxygen microelectrode most advanced and sophisticated slowly close to biological membrane, when microelectrode tip arrives biofilm surface, the reading of record dissolved oxygen microelectrode main frame display, after this each perpendicular to biological membrane direction stepping 20 μm, and record biological membrane different-thickness place dissolved oxygen concentration, by that analogy, the inner dissolved oxygen concentration space distribution of difference place, test organisms film surface biological membrane, and 4 rank fitting of a polynomials are carried out to dissolved oxygen concentration distribution curve, then second-order differential is carried out to matched curve, obtain the second derivative of the inner dissolved oxygen concentration of biological membrane with biofilm thickness change curve, biological membrane aerobic layer thickness and anaerobic layer thickness is calculated finally by this curve.
Measure the method for biological membrane aerobic layer thickness and anaerobic layer thickness, use the device of said determination biological membrane aerobic layer thickness and anaerobic layer thickness, carry out in accordance with the following steps:
S1: artificial distribution, use ammonium bicarbonate, dipotassium hydrogen phosphate and sodium bicarbonate configuration waste water, the pH value regulating waste water is 8.0, and the waste water 4L loading volume getting configuration is in the described wide-necked bottle of 5L;
S2: the gas in oxygen bottle and nitrogen cylinder drawn and passes into after mixing in the waste water in wide-necked bottle, opening aeration tube dish 7 and carry out aeration, open the first magnetic stirring apparatus 8 and stir, regulating the oxygen concentration of the waste water solution in wide-necked bottle to 2.5mg/L;
S3: the waste water in wide-necked bottle is caused biological membrane test trough 2 by peristaltic pump 3 with plastic tube, after waste water is full of biological membrane test trough 2, draws the waste water in biological membrane test trough 2 and loops back in wide-necked bottle;
Open the second magnetic stirring apparatus bottom biological membrane test trough 2 and the 3rd magnetic stirring apparatus, waste water in biological membrane test trough 2 is stirred, the portable dissolved oxygen instrument 6 of band plug is inserted and secured in biological membrane test trough 2, oxyty in test organisms film test trough 2, until in biological membrane test trough 2, waste water oxyty is consistent with waste water oxyty in wide-necked bottle, and be stabilized in 2.5 mg/L;
S4: choose fritter biological membrane and put into test trough from one-step autotrophic nitrogen removal system, and be fixed on the upper surface of block rubber 4 with the nylon wire of macropore, dissolved oxygen micro electrode 5 is inserted biological membrane test trough 2, with the inner microcell dissolved oxygen distribution of test organisms film.
In step S1, the water distribution water quality of the waste water configured is as following table:
| Ammonia nitrogen (mg/L) | TP（mg/L） | Microelement nutritious liquid (ml/L) | pH | DO（mg/L） |
| 200 | 5 | 2 | 8.0 | 2.5 |
Wherein shown in microelement nutritious liquid table composed as follows:
| Trace element | Concentration (g/L) |
| EDTA | 5.0 |
| CoCl 2·6H 2O | 1.6 |
| ZnSO 4·7H 2O | 2.2 |
| MnCl 2·4H 2O | 5.1 |
| CuSO 4·5H 2O | 1.6 |
| (NH 4) 6Mo 7O 24·4H 2O | 1.1 |
| CaCl 2·2H 2O | 5.5 |
| FeSO 4·7H 2O | 5.0 |
Wherein DO refers to dissolved oxygen DO, i.e. the milligram number of oxygen in every premium on currency.TP(total phosphorus, total phosphorus), the phosphorus-containing compound in water is mainly divided three classes: orthophosphate, condensed phosphate and organophosphorus; The phosphorus of form various in water is converted into orthophosphate by clearing up, and the data recorded are total phosphorus.
In described step S4, the step of the inner microcell dissolved oxygen distribution of test organisms film is: by the most advanced and sophisticated locus of three-dimensional micro-electrode propeller control dissolved oxygen micro electrode 5, make the tip of dissolved oxygen micro electrode 5 slowly close to biological membrane, when the tip of dissolved oxygen micro electrode 5 arrives biofilm surface, the reading of record dissolved oxygen DO Test Host display, after this, dissolved oxygen micro electrode 5 is each perpendicular to biological membrane direction stepping 20 μm, dissolved oxygen DO Test Host calculates biological membrane different-thickness place dissolved oxygen concentration, by that analogy, the inner dissolved oxygen concentration distribution of test organisms film, calculate biological membrane aerobic layer thickness and anaerobic layer thickness.
The computing method of described biological membrane different-thickness place dissolved oxygen concentration are: according to law of conservation of mass, under lower state:
The amount that the amount that amount=diffusion rate of dissolved oxygen that diffusion rate of dissolved oxygen enters goes out+dissolved oxygen DO reaction consumes
The amount diffused into is:
The amount diffused out is:
The amount that reaction consumes:
Then have:
（1）
Arrange:
（2）
Wherein
cfor oxyty, unit is g/m
3;
dfor dissolved oxygen DO is at biological membrane intraparticle diffusivity, unit is m
2/ s; OUR is the wear rate of dissolved oxygen DO, and unit is g/ (m
3.s);
zfor biofilm thickness, unit is m.
The microorganism of biomembranous extexine is generally aerobic bacteria, is thus called aerobic layer.Internal layer is affected and supplies hypoxgia because of the diffusion of oxygen, and anaerobion amount reproduction is called anaerobic layer.When oxygen update rate is greater than 0 g/ (m
3.s) time, can think that this place's biological membrane has aerobic microbiological activity, when oxygen update rate is less than or equal to 0 g/ (m
3.s), time, can think that this place's biological membrane is active without aerobic microbiological.Therefore, biological membrane oxygen consumption and anaerobic conditions can be judged by oxygen update rate in the distribution of biological membrane inside, biological membrane extexine oxygen update rate is greater than 0 g/ (m
3.s) biofilm thickness is defined as aerobic layer, and internal layer oxygen update rate is less than or equal to 0 g/ (m
3.s) be defined as anaerobic layer.
From equation (2), the wear rate of dissolved oxygen DO in biological membrane inside is relevant with the second derivative of the inner dissolved oxygen concentration distribution curve of biological membrane, and when the second derivative of dissolved oxygen concentration distribution curve is greater than 0, oxygen update rate is greater than 0 g/ (m
3.s), when the second derivative of dissolved oxygen concentration distribution curve is less than or equal to 0, oxygen update rate is less than or equal to 0 g/ (m
3.s).Therefore, by second derivative determination biological membrane aerobic layer thickness and the anaerobic layer thickness of the inner dissolved oxygen concentration distribution curve of biological membrane.Namely the second derivative of biological membrane extexine dissolved oxygen concentration distribution curve is greater than 0 place is aerobic layer, and the second derivative of internal layer dissolved oxygen concentration distribution curve is less than or equal to 0 g/ (m
3.s) be anaerobic layer.
Fig. 3, Fig. 4 and Fig. 5 are respectively inner dissolved oxygen concentration distribution (measured value) in biological membrane 3 diverse location places, matched curve and second derivative figure.As can be seen from the figure, the thickness that the outer second derivative of three diverse location biological membranes is greater than 0 is respectively 360,250 and 210 μm.Therefore this biological membrane three diverse location place aerobic layer thickness are respectively 360,250 and 210 μm.Below the outer aerobic layer of biological membrane, it is anaerobic layer that second derivative is less than or equal to 0 place, and thickness is respectively 150,400 and 470 μm.By measuring a large amount of diverse location places aerobic layer thickness and anaerobic layer thickness, and its mean value can be got, being this biological membrane aerobic layer and anaerobic layer thickness.Institute's location is put more, survey biological membrane aerobic layer and anaerobic layer thickness is more accurate.
The above embodiment of the present invention is only for example of the present invention is described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, other multi-form change and variations can also be made on the basis of the above description, cannot give all embodiments exhaustive here.Every belong to technical scheme of the present invention the apparent change of amplifying out or variation be still in the row of protection scope of the present invention.
Claims (6)
1. one kind measures the device of biological membrane aerobic layer thickness and anaerobic layer thickness, it is characterized in that: comprise oxygen conveying device, first container (1) of waste water is housed and the biological membrane test trough (2) of waste water is housed, described first container (1) is provided with draft tube, rising pipe and venthole, under the draft tube of this first container (1) and the lower end of rising pipe are all positioned at the waste water liquid level of this first container (1), the outlet side of described oxygen conveying device is connected with the draft tube of described first container (1), also comprise peristaltic pump (3), the rising pipe of described first container (1) is connected with the water inlet end of peristaltic pump (3), the water side of this peristaltic pump (3) is communicated by the inside of water pipe with described biological membrane test trough (2), this biological membrane test trough (2) is also provided with return pipe and communicates with described first container (1),
Also comprise block rubber (4), this block rubber (4) is arranged on described biological membrane test trough (2) bottom, waste water liquid level in described biological membrane test trough (2), higher than the end face of described block rubber (4), the end face of this block rubber (4) is also provided with for the biomembranous nylon wire of fixed placement at block rubber end face;
Also comprise dissolved oxygen micro electrode system and portable dissolved oxygen instrument (6), described dissolved oxygen micro electrode system comprises dissolved oxygen micro electrode (5), three-dimensional micro-electrode thruster and dissolved oxygen DO Test Host, the biofilm contact of described dissolved oxygen micro electrode (5) by the perforate at described biological membrane test trough (2) top and the end face of described block rubber (4) are arranged, described three-dimensional micro-electrode thruster is for promoting described dissolved oxygen micro electrode (5) stepping, described dissolved oxygen DO Test Host is used for calculating the data that described dissolved oxygen micro electrode (5) receives, process and display, described portable dissolved oxygen instrument (6) is arranged on described biological membrane test trough (2), and the bottom of this portable dissolved oxygen instrument (6) is arranged in the waste water of described biological membrane test trough (2).
2. the device of mensuration biological membrane aerobic layer thickness according to claim 1 and anaerobic layer thickness, it is characterized in that, described oxygen conveying device comprises a band reduction valve and is filled with the oxygen bottle of purity oxygen, a band reduction valve and be filled with the nitrogen cylinder of pure nitrogen gas, two flowmeters (11) and gas mixer chamber (12), described oxygen bottle is connected the input end of a flowmeter (11) respectively with the output terminal of nitrogen cylinder, the output terminal of these two flowmeters (11) is connected with two air intake openings of described gas mixer chamber (12) successively, the gas outlet of described gas mixer chamber (12) is connected with the draft tube of described first container (1).
3. the device of mensuration biological membrane aerobic layer thickness according to claim 1 and anaerobic layer thickness, it is characterized in that, described first container (1) is the wide-necked bottle of band plug, the volume of this wide-necked bottle is 5L, plug on described wide-necked bottle arranges three through holes, wherein first through hole is installed with the draft tube of described first container (1), and second through hole is installed with the rising pipe of described first container (1), and the 3rd through hole is used for wide-necked bottle inner chamber to be communicated with ambient atmosphere.
4. the device of mensuration biological membrane aerobic layer thickness according to claim 1 and anaerobic layer thickness, it is characterized in that, described portable dissolved oxygen instrument (6) is cylindrical, this portable dissolved oxygen instrument (6) cover has rubber plug, the top of described biological membrane test trough (2) is provided with and coordinates with described rubber plug, for described portable dissolved oxygen instrument (6) being arranged in the mounting hole on described biological membrane test trough (2).
5. the device of mensuration biological membrane aerobic layer thickness according to claim 3 and anaerobic layer thickness, it is characterized in that, the aeration tube dish (7) being arranged in waste water is provided with in described wide-necked bottle, the air intake opening of this aeration tube dish (7) is connected with the end of the draft tube of described first container (1), and the below of described aeration tube dish (7) is provided with the first magnetic stirring apparatus (8).
6. the device of mensuration biological membrane aerobic layer thickness according to claim 1 and anaerobic layer thickness, it is characterized in that, the bottom of described biological membrane test trough (2) is provided with the second magnetic stirring apparatus (9) and the 3rd magnetic stirring apparatus (10), and this second magnetic stirring apparatus (9) and the 3rd magnetic stirring apparatus (10) are positioned at the both sides of described block rubber (4).
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| CN103808284B (en) * | 2014-03-04 | 2016-05-18 | 中国科学院重庆绿色智能技术研究院 | A kind of device and method of in-site detecting wastewater treatment biomembrane interior spatial structure |
| CN103969302B (en) * | 2014-04-14 | 2016-04-06 | 杭州电子科技大学 | A kind of method measuring dissolved oxygen coefficient of diffusion in biological membrane |
| CN105758921B (en) * | 2016-03-31 | 2019-01-01 | 中国科学院重庆绿色智能技术研究院 | A kind of method of material concentration dynamic change in quasi- in-situ monitoring biomembrane |
| CN106018481B (en) * | 2016-05-17 | 2018-09-21 | 中国科学院生态环境研究中心 | A kind of alternate biomembrane microenvironment detection method of gas-liquid two-phase |
| CN105928982B (en) * | 2016-05-17 | 2018-07-17 | 中国科学院生态环境研究中心 | A kind of alternate biomembrane microenvironment detection device of gas-liquid two-phase |
| CN107515208B (en) * | 2017-09-14 | 2023-08-04 | 浙江省城乡规划设计研究院 | Method for detecting dissolved oxygen in aerobic biomembrane based on fluorescence quenching, and special calibration device and measuring device |
| CN109489568B (en) * | 2018-09-30 | 2019-10-08 | 南京大学 | A kind of auxiliary device and measuring method of on-line determination Wastewater treatment filling material biofilm thickness |
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