CN106526114A - Sewage biological toxicity analysis method based on immobilized aerobic microbial system - Google Patents
Sewage biological toxicity analysis method based on immobilized aerobic microbial system Download PDFInfo
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- CN106526114A CN106526114A CN201610963761.6A CN201610963761A CN106526114A CN 106526114 A CN106526114 A CN 106526114A CN 201610963761 A CN201610963761 A CN 201610963761A CN 106526114 A CN106526114 A CN 106526114A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Abstract
The invention discloses a sewage biological toxicity analysis method based on immobilized aerobic microbial system. The method comprises the following steps: 1) a semi-soft biological filler with certain length is placed in a sewage processing factory aerobic biochemical pool, and immersed for 15-20 days for aeration culture, microbe immobilization is completed; 2) the sewage processing factory sewage is subjected to pre-aeration treatment according to periodicity, then is injected to an immobilized aerobic microbe reaction system for reaction, and sewage is periodically and intermittently injected for the reaction; and 3) a DO sensor in a reactor is used to obtain the DO value to a periodically and regularly changed system for sewage biological toxicity analysis. Through comparison of DO value and a standard delta HDO, as well as biological toxicity inhibition verification flow, biological toxicity degree of the sewage can be rapidly and accurately evaluated, at the same time, a semi-soft filler immobilized microbe construction reaction system mode is employed for providing more stable environment for microbe growth and metabolism, and disturbance and loss on microbe during water discharge and injection processes can be greatly reduced.
Description
Technical field
The present invention relates to the technical field of sewage monitoring, more particularly to biological fixnig be based on microorganism system dissolved oxygen
The saprobia toxicity assay of (Dissolved Oxygen, DO) value.
Background technology
Saprobia Toxicity Monitoring technology has：(1) aquatic animals and plants acute toxicity test, including toxicity in fish, water flea class
The test method(s)s such as toxicity, Herba Spirodelae class, Root Tip Cells of Vicia Faba micronucleus, algae toxicity；(2) Ecotoxicology test, including the characteristics of luminescence, breathing
The test method(s)s such as suppression, enzyme activity suppression.Wherein, the features such as microbial respiratory inhibition test is due to its accuracy and intuitive,
It is used to evaluate toxic action of the waste water to activated sludge.When processing system causes sludge to be poisoned toxic impact is subjected to,
It is often early warning the most sensitive that the oxygen breathing rate of sludge declines suddenly.
At present, in based on the sewage toxicity online measuring technique of microbial respiratory, mainly by microorganism in suspended sludge
Ratio breathing rate change come the biological activity and sewage treatment capacity analyzed with judge sludge system.For example Chinese invention is special
200910082957.4 (Publication No. CN 101556270A) of profit disclose a kind of monitoring activated Sludge System dynamic than oxygen consumption speed
The device of rate, the invention are will to seal recorder jar, are placed on magnetic stirring apparatuss, form sludge circulation by reflux pump and reactor
Loop, the device design are succinct, stable and measurement data favorable reproducibility, by intuitively stating SOUR curves, can be with standard
True monitoring system reactive state not in the same time.Although on-line checking specific oxygen utilization rate is more quicker than conventional sense technology, straight
See with it is accurate, but as specific oxygen utilization rate needs to be turned by the calculating of formula according to system dissolved oxygen and suspended sludge concentration values
Change can just be obtained, rather than data that instrument is directly monitored online, therefore, its during practical operation with application, with number
Easily there is the situation of non-correlation according to simulation, cause resultant error, meanwhile, the aerobic life of current most domestic industrial wastewater
Thing handling process is to adopt contact oxidation method, and by biologic packing material enriched microorganism, in Aeration tank, suspended sludge amount is few, difficult
To meet continuous demands of the saprobia Toxicity Monitoring to suspended active sludge.
The content of the invention
Present invention is primarily targeted at overcoming the shortcoming of prior art with not enough, one immobilization aerobic microbiological of structure
System, is suppressed when bio-toxicity material is invaded according to microorganism respiration of oxygen consumption, and under constant reaction condition, micro- life
Thing breathing rate and feature of the reaction system DO value changes in anti-correlation, there is provided a kind of to be reacted based on immobilization aerobic microbiological
The sewage toxicity analysis method of system DO value tag tracing analysiss.
In order to achieve the above object, the present invention is employed the following technical solutions：
The invention provides a kind of saprobia toxicity assay based on immobilization aerobic microbiological system, including under
State step：
S1, by the semi-soft biologic packing material input sewage treatment plant aerobic biochemical pool submergence of certain length, through 15-20 days
Aeration culture, the complete attachment activity sludge of semi-soft biologic packing material, completes microbial immobilized；
S2, the filler of immobilized microorganism is put in the reactor for fill with clear water the aeration for carrying out constant amount of air；
S3, sewage treatment plant's actual sewage press the pre-exposure gas disposal of cycle Jing, and its DO value is close to the micro- life of immobilization in reactor
During objects system DO values, add to reactor and react with immobilized microorganism, each cycle injection constant volume sewage, a cycle knot
Shu Hou, reactor discharge the water with injection sewage same volume, reinject the constant volume sewage of Jing preaeration again, with this cycle
Sexual refractoriness injection sewage is reacted；
In S4, reactor, DO sensor acquisition obtains DO value tag curve of the reaction system in periodic regularity change.
As preferred technical scheme, in step S1, the semi-soft biologic packing material is the semi-soft biologic packing material of snowflake piece.
As preferred technical scheme, in step S2, also comprise the steps：Constant amount of air aeration, observing response device
DO values, when DO values are no longer raised, and maintain a concentration level, immobilized microorganism system reaches satisfaction injection sewage reaction
Requirement；
Used as preferred technical scheme, step S3 is specially：First by actual sewage by volume cycle successively from small to large
Property Jing pre-exposure gas disposals, DO values are injected into reactor reaction after being close to reactor DO values, reaction system DO value tag curve declines
Area fall △ HDO↓When reaching maximum, corresponding injection sewage volume is defined as the constant period injected slurry volume V of sewageNote, press
The periodicity continuous service of reaction system is carried out according to the sewage injected slurry volume, reaction process is in constant reaction system volume, constant
Temperature, constant wind supply quantity, are carried out under conditions of constant sewage cyclic injection amount.
Used as preferred technical scheme, the DO value tags curve includes declining area, stable region, rising area and baseline stability
Four, area characteristic, wherein declining area fall △ HDO↓It is most important feature analysis data, will determines that cyclic waterflooding is permanent
Determine the reaction system maximum △ H gathered during volumeDO↓As standard △ HDO↓It is designated as △ HDO ↓-mark, in constant reaction system volume, temperature
Under degree, air demand and cyclic waterflooding volume conditions, when the sewage of cyclic injection is stable in properties, each cycle DO value tag curves
Keep highly consistent, △ HDO↓Closely, variance rate is within 10%, and the sewage containing bio-toxicity is according to identical flow process
After injecting reactor, immobilized microorganism respiration of oxygen consumption is suppressed, before DO values curve law feature quilt under normal circumstances
Destruction, △ HDO↓It is obviously reduced, sets △ HDO↓It is reduced to △ HDO ↓-markHalf is △ HDO↓≤ 50% △ HDO ↓-markShi Qidong sewage is given birth to
Thing toxicity verifies flow process, now injects a certain amount of nutritional solution containing fast degraded biologically carbon source in reactor, continues collection
Reaction system DO value, if DO values restart continuous decrease after reaction system injection nutritional solution, checking immobilized microorganism is not
By the high inhibition of bio-toxicity material, it is more likely that as the load for injecting sewage is greatly reduced, if DO values are in reaction
Do not restart to decline but rising in setting time T after system injection nutritional solution, checking immobilized microorganism breathing is subject to
Bio-toxicity material suppresses, and the sewage of injection is identified as sewage containing bio-toxicity.
Used as preferred technical scheme, setting time T is 2 minutes.
The present invention compared with prior art, has the advantage that and beneficial effect：
The present invention is formed using the analytical technology exploitation based on immobilization aerobic microbiological reaction system DO value changes feature,
When the sewage containing bio-toxicity enters detection means, the change of reaction system DO value will present enters fashionable with normal sewage
Different trend features, this variation tendency are the performances directly perceived that microorganism respiration of oxygen consumption is suppressed, by with standard △ HDO
↓ relatively in addition bio-toxicity suppress checking flow process, can quick and precisely assess the bio-toxicity degree of sewage, while adopting medium-soft
Property filler immobilized microorganism build the mode of reaction system, the growth metabolism for microorganism provides more stable environment, greatly
Width reduces disturbance and the loss that reaction system periodical discharge the injecting process is caused to microorganism, also solves this method and is adopting
The limitation that cannot be applied due to not collecting suspended active sludge with the industrial effluent treatment plant of catalytic oxidation bioprocess technology.
Description of the drawings
Fig. 1 is the flow chart of the present invention；
Fig. 2 is reaction system DO characteristic curve schematic diagram；
Fig. 3 is that bio-toxicity sewage enters rear reaction system DO characteristic curve change schematic diagram.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment
As shown in figure 1, saprobia poison of the present embodiment based on immobilization aerobic microbiological reaction system dissolved oxygen concentration
Property analysis method is as follows, and the semi soft packing of certain length is put into 15-20 submerged culture to sewage treatment plant's bio-aeration pool
My god, it is microbial immobilized after the completion of, filler is put into in the reactor for fill clear water permanent tolerance aeration, first by actual sewage by
Periodicity is injected into reactor reaction Jing after pre-exposure gas disposal to volume successively from small to large, and reaction system DO value tag curve declines
During area fall △ HDO ↓ reach maximum, corresponding injection sewage volume is defined as the constant period injected slurry volume V notes of sewage,
Reaction process enters under conditions of constant reaction system volume, steady temperature, constant wind supply quantity, constant sewage cyclic injection amount
OK.The DO characteristic curves of DO sensors real-time online collection reaction system are analyzed for saprobia Toxicity Monitoring.
As shown in Figures 2 and 3, in the present embodiment, saprobia toxicity assay is as follows, and DO characteristic curves include declining
Area, stable region, rising area and four, baseline stability area characteristic, the reactant that will be gathered when determining cyclic waterflooding constant volume
System maximum △ HDO ↓ as standard △ HDO ↓ be designated as △ HDO ↓-mark, in constant reaction system volume, temperature, air demand and cycle
Under water filling volume conditions, when the sewage of cyclic injection is stable in properties, each cycle DO value tags curve keeps highly consistent, △
HDO ↓ closely, variance rate within 10%, and after the sewage containing bio-toxicity is according to identical flow process injecting reactor, Gu
Surely change microorganism respiration of oxygen consumption to be suppressed, DO values curve law feature is destroyed under normal circumstances before, △ HDO ↓ substantially
Reduce, this method setting △ HDO ↓ be reduced to △ HDO ↓-mark half i.e. △ HDO ↓≤50% △ HDO ↓-timestamp startup saprobia
Toxicity verifies flow process, and the nutritional solution of a certain amount of carbon source containing fast degraded biologically (sodium acetate) is now injected in reactor, after
Continuous collection reaction system DO value, if DO values restart continuous decrease after reaction system injection nutritional solution, checking immobilization is micro-
Biology is not affected by the high inhibition of bio-toxicity material, it is more likely that as the load for injecting sewage is greatly reduced, if DO values
Do not restarted to decline but rising in 2 minutes, checking immobilized microorganism breathing is received after reaction system injection nutritional solution
Suppress to bio-toxicity material, the sewage of injection is identified as sewage containing bio-toxicity.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment
Limit, other any spirit without departing from the present invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (6)
1. a kind of saprobia toxicity assay based on immobilization aerobic microbiological system, it is characterised in that including following
Step：
S1, by the semi-soft biologic packing material input sewage treatment plant aerobic biochemical pool submergence of certain length, through 15-20 days aerations
Culture, the complete attachment activity sludge of semi-soft biologic packing material, completes microbial immobilized；
S2, the filler of immobilized microorganism is put in the reactor for fill with clear water the aeration for carrying out constant amount of air；
S3, sewage treatment plant's actual sewage press the pre-exposure gas disposal of cycle Jing, and its DO value is close to immobilized microorganism body in reactor
When being DO values, add to reactor and react with immobilized microorganism, each cycle injection constant volume sewage, a cycle terminate
Afterwards, reactor discharges the water with injection sewage same volume, reinjects the constant volume sewage of Jing preaeration again, with this periodically
It is intermittently injecting sewage to be reacted；
In S4, reactor, DO sensor acquisition obtains DO value tag curve of the reaction system in periodic regularity change.
2. saprobia toxicity assay according to claim 1 based on immobilization aerobic microbiological system, its feature
It is that, in step S1, the semi-soft biologic packing material is the semi-soft biologic packing material of snowflake piece.
3. saprobia toxicity assay according to claim 1 based on immobilization aerobic microbiological system, its feature
It is, in step S2, also to comprise the steps：Constant amount of air aeration, observing response device DO values, when DO values are no longer raised, dimension
Hold in a concentration level, immobilized microorganism system reaches the requirement for meeting injection sewage reaction.
4. saprobia toxicity assay according to claim 1 based on immobilization aerobic microbiological system, its feature
It is that step S3 is specially：First by actual sewage by volume periodicity Jing pre-exposure gas disposal successively from small to large, DO values are close to
Reactor reaction is injected into after reactor DO values, reaction system DO value tag curve declines area fall △ HDO↓Reach maximum
When corresponding injection sewage volume be defined as the constant period injected slurry volume V of sewageNote, reacted according to the sewage injected slurry volume
The periodicity continuous service of system, reaction process is in constant reaction system volume, steady temperature, constant wind supply quantity, constant sewage
Carry out under conditions of cyclic injection amount.
5. saprobia toxicity assay according to claim 1 based on immobilization aerobic microbiological system, its feature
It is that the DO value tags curve includes declining area, stable region, rising area and four, baseline stability area characteristic, wherein under
Drop area fall △ HDO↓It is most important feature analysis data, the reactant that will be gathered when determining cyclic waterflooding constant volume
System maximum △ HDO↓As standard △ HDO↓It is designated as △ HDO ↓-mark, in constant reaction system volume, temperature, air demand and cyclic waterflooding
Under volume conditions, when the sewage of cyclic injection is stable in properties, each cycle DO value tags curve keeps highly consistent, △ HDO↓
Closely, variance rate is within 10%, and after the sewage containing bio-toxicity is according to identical flow process injecting reactor, immobilization
Microorganism respiration of oxygen consumption is suppressed, and DO values curve law feature is destroyed under normal circumstances before, △ HDO↓It is obviously reduced,
Setting △ HDO↓It is reduced to △ HDO ↓-markHalf is △ HDO↓≤ 50% △ HDO ↓-markShi Qidong saprobias toxicity verifies flow process, now
A certain amount of nutritional solution containing fast degraded biologically carbon source is injected in reactor, continues collection reaction system DO value, if DO values
Restart continuous decrease after reaction system injection nutritional solution, checking immobilized microorganism is not affected by the strong of bio-toxicity material
Suppress, it is more likely that as the load for injecting sewage is greatly reduced, if DO values are being set after reaction system injection nutritional solution
The T that fixes time is interior not to restart to decline but rising, and the breathing of checking immobilized microorganism is by the suppression of bio-toxicity material, injection
Sewage be identified as sewage containing bio-toxicity.
6. saprobia toxicity assay according to claim 5 based on immobilization aerobic microbiological system, its feature
It is that setting time T is 2 minutes.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231438A (en) * | 2018-11-07 | 2019-01-18 | 山东建筑大学 | A kind of saprobia toxicity on-line monitoring early warning system |
| CN110426498A (en) * | 2019-08-08 | 2019-11-08 | 广州市净水有限公司 | A kind of saprobia methods for toxicity monitoring |
| CN115798625A (en) * | 2023-01-17 | 2023-03-14 | 中国科学技术大学 | Reaction flow numerical solving method coupled with single-step chemical mechanism |
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| CN101928061A (en) * | 2010-04-16 | 2010-12-29 | 中山大学 | Surface porous suspended biological filler, and preparation method and application thereof |
| CN102944658A (en) * | 2012-11-28 | 2013-02-27 | 广州中国科学院沈阳自动化研究所分所 | Method for analyzing biotoxicity of sewage based on system dissolved oxygen concentration |
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|---|---|---|---|---|
| CN101928061A (en) * | 2010-04-16 | 2010-12-29 | 中山大学 | Surface porous suspended biological filler, and preparation method and application thereof |
| CN102944658A (en) * | 2012-11-28 | 2013-02-27 | 广州中国科学院沈阳自动化研究所分所 | Method for analyzing biotoxicity of sewage based on system dissolved oxygen concentration |
Non-Patent Citations (3)
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| 《ANNUAL INTERNATIONAL CONFERENCE ON CYBER TECHNOLOGY IN AUTOMATION CONTROL AND INTELLIGENT SYSTEMS》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231438A (en) * | 2018-11-07 | 2019-01-18 | 山东建筑大学 | A kind of saprobia toxicity on-line monitoring early warning system |
| CN110426498A (en) * | 2019-08-08 | 2019-11-08 | 广州市净水有限公司 | A kind of saprobia methods for toxicity monitoring |
| CN115798625A (en) * | 2023-01-17 | 2023-03-14 | 中国科学技术大学 | Reaction flow numerical solving method coupled with single-step chemical mechanism |
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Application publication date: 20170322 |