CN103146765A - Alcohol from maple hemicellulose and preparation method of alcohol - Google Patents
Alcohol from maple hemicellulose and preparation method of alcohol Download PDFInfo
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- CN103146765A CN103146765A CN2013100577404A CN201310057740A CN103146765A CN 103146765 A CN103146765 A CN 103146765A CN 2013100577404 A CN2013100577404 A CN 2013100577404A CN 201310057740 A CN201310057740 A CN 201310057740A CN 103146765 A CN103146765 A CN 103146765A
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- hemicellulose
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention provides an alcohol from maple hemicellulose and a preparation method of the alcohol and relates to the technical fields of fuel alcohol and fermentation. The preparation method comprises the following two processes: raw material preprocessing and alcohol fermentation. According to the preparation method, grinded maple sieved by a 0.5-millimetre sieve is used as a raw material, 0.5%v/v oxalic acid is used as a liquid phase, the solid concentration is 10%W/V, and the maple is preprocessed for 35 minutes at the temperature of 160 DEG C. After the preprocessing, the temperature is reduced to 25 DEG C, filtering is carried out, supernate is taken, the pH is adjusted to 7.5 by use of 2mol/L KOH, a 10g/L yeast extract and 20g/L peptone are added, escherichia coli K011 is introduced, and hemicellulose alcohol fermentation is carried out at the temperature of 37 DEG C. The yield of the alcohol obtained by fermentation under the process parameters achieves about 94% of theoretical yield. By adopting the production technology, loss of the hemicellulose can be obviously reduced, the yield of the alcohol can be improved, and the production technology has high economic benefit and social benefit.
Description
Technical field
The present invention relates to a kind of ethanol that derives from the maple hemicellulose and preparation method thereof, belong to biomass energy and fermentation technology for production of fuel ethanol field.
Background technology
Alcohol fuel refers to that generally volumetric concentration reaches the dehydrated alcohol more than 99.5%, can use in the ethanol engine of special use; Can mix with gasoline according to a certain percentage again, directly use under the prerequisite of former petrol engine not being done any change.Can be used as on the one hand the surrogate of gasoline to reduce petrol consumption, can reduce on the other hand the discharging of automobile exhaust pollution thing, improve city atmospheric environment.
Traditional ethanol fermentation is take sugar or starch as raw material.But the main source of sugar and starch is food, world food has been consisted of safely threat take grain as the raw material production alcohol fuel, replaces grain in the urgent need to finding other raw materials.So the fuel ethanol production take lignocellulose as raw material becomes the focus that various countries are competitively studied.In lignocellulose, after hemicellulose came Mierocrystalline cellulose as the second abundant carbohydrate, its content was up to more than 35%.Up to the present, at first the production of biomass fuel ethanol carry out pre-treatment mainly take Mierocrystalline cellulose as starting material, then must finely cross enzymolysis, just can discharge glucose and then carry out ethanol fermentation.But after the hemicellulose pre-treatment, get final product to get Soluble Monosaccharide and directly by microbial fermentation.Due to these reasons, the hemicellulose bio-transformation is should be not unheeded.Utilize hemicellulose to produce ethanol, finally can improve the whole utilising efficiency of wood materials.The present invention adopts organic acid pre-treatment combining with fermentation process to mend the fuel ethanol production technique means that permits nitrogenous source, to improving China's fuel ethanol production level, promotes the development of Biomass Energy Industry, and important practical significance is arranged.
Summary of the invention
The invention provides a kind of ethanol that derives from the maple hemicellulose and preparation method thereof.The method can significantly reduce the loss of monose in preprocessing process, shorten fermentation time, improves ethanol production.
Technical solution of the present invention is as follows:
(1) the maple material is ground, cross 0.5 mm sieve.And starting materials is carried out composition analysis, comprise content of cellulose, hemicellulose level and ash content;
(2) organic acid pretreatment process degraded maple lignocellulosic material: take 0.5%v/v oxalic acid as liquid phase, solids concn 10%W/V, after soaking in advance 12 hours under 25 ℃, 160 ℃ of pre-treatment 35 minutes.After pre-treatment, be cooled to 25 ℃, vacuum filtration is got supernatant;
(3) pretreated hemicellulose hydrolysate is carried out composition analysis, the composition of required analysis comprises Xylose Content, glucose content, furfural and hydroxymethylfurfural.
(4) adjust hemicellulose hydrolysate pH to 7.5 with 2mol/LKOH, add yeast extract 10g/L and peptone 20g/L, access intestinal bacteria K011,37 ℃ are carried out half fermenting cellulosic ethanol.
(5) detect Xylose Content, glucose content and ethanol content in fermented liquid.
Embodiment
The present invention is a kind of novel method that improves the biomass fuel ethanol production, mainly is comprised of pre-treatment and fermentation two large steps.Below by specific embodiment, the present invention is described in further detail.The preparation method is as follows:
1 pretreatment:
(1) the maple material grinds, and crosses the 0.5mm sieve, carries out composition analysis: accurately take sample 0.5g, as for the 10ml small beaker, add 72% sulfuric acid 3.00ml, stir, 30 ℃ of water-bath 1h.Concentrated acid hydrolysis liquid is transferred in the 100ml vial, and the concentration to 4% of dilute acid.Reclaim reference liquid as internal reference take sugar simultaneously, same dilute acid concentration seals in vial.Be placed in simultaneously in high-pressure sterilizing pot, under 121 ℃, insulation 45min.The clumsy filter of sand core funnel.Get 5.00ml filtrate, add excess amount of Ca CO
3Regulate the pH value, cross the leaching supernatant.Measure contents of monosaccharides in supernatant liquor by HPLC.After sugared rate of recovery conversion, glucose content multiply by coefficient 0.90 and gets content of cellulose, and wood sugar and pectinose content sum multiply by coefficient 0.88 and get hemicellulose level.Residue after filter, distilled water wash is to neutral, and 105 ℃ of oven dry 550 ℃ of lower 4h ashing, get ash oontent;
(2) take the maple of grinding as material, take 0.5%v/v oxalic acid as liquid phase, solids concn 10%W/V soaked 12 hours under 25 ℃ in advance, 160 ℃ of pre-treatment 35 minutes.After pre-treatment, be cooled to 25 ℃, vacuum filtration gets the maple pretreatment fluid;
(3) gained maple pretreatment fluid is added 4% sulfuric acid.Reclaim the accurate liquid of material as internal reference take sugar simultaneously, same dilute acid concentration, in vial, sealing.Be placed in high-pressure sterilizing pot, under 121 ℃, insulation 45min.Get 5.00ml liquid, add excess amount of Ca CO
3Regulate the pH value, cross the leaching supernatant.Measure contents of monosaccharides in supernatant liquor by HPLC.Measured Xylose Content in the Xylose Content in original composition and pretreatment fluid relatively, pre-treatment is really imitated as can be known.
The fermentation of 2 pretreatment fluids: at first adjust hemicellulose hydrolysate pH to 7.5 with 2mol/L KOH, add yeast extract 10g/L and peptone 20g/L, by concentration 5g bacterial strain weight in wet base/L access intestinal bacteria K011,37 ℃ are carried out half fermenting cellulosic ethanol.Detect Xylose Content in fermented liquid, glucose content and ethanol content, hemicellulose hydrolysate fermentation efficiency as can be known with HPLC.
The present invention can get maximum ethanol production in 24 hours under aforesaid operations, reach 93.9% of theoretical yield, and the ethanol final concentration is 9.0g/L.
the above, it is only preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, although the present invention discloses as above with preferred embodiment, yet be not to limit the present invention, any those skilled in the art, in the scope that does not break away from technical solution of the present invention, when the technology contents that can utilize above-mentioned announcement is made a little change or is modified to the equivalent embodiment of equivalent variations, in every case be not break away from the technical solution of the present invention content, any simple modification that foundation technical spirit of the present invention is done above embodiment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. hemicellulose ethanol fermentation technology is characterized in that half fiber hydrolysate of gained after the pre-treatment of maple organic acid not needing detoxification treatment, directly adjust the pH value, add the factors stimulated growth thing, can the ferment bacterial strain of five-carbon sugar of access, 37 ℃ are carried out ethanol fermentation.
2. by hemicellulose ethanol fermentation technology claimed in claim 1, its maple material characteristics is crossed 0.5 mm sieve for after grinding.
3. by hemicellulose ethanol fermentation technology claimed in claim 1, organic acid is 0.5%v/v oxalic acid.
4. by hemicellulose ethanol fermentation technology claimed in claim 1, the preparation method of half fiber hydrolysate and step are: take 0.5%v/v oxalic acid as liquid phase, solids concn 10%W/V soaked 12 hours under 25 ℃ in advance, 160 ℃ of pre-treatment 35 minutes.After pre-treatment, be cooled to 25 ℃, vacuum filtration gets hemicellulose hydrolysate.
5. by hemicellulose ethanol fermentation technology claimed in claim 1, the preparation method of factors stimulated growth thing and step are: Sumizyme MP is at pH2.2, and temperature 60 C is hydrolyzed 2.0% whey-protein, enzyme-to-substrate obtains the short skin mixture of 200D-1500D than 7.0%.
6. by hemicellulose ethanol fermentation technology claimed in claim 1, its fermentation process is: at first adjust pH to 7.5 with 2mol/LKOH, the nitrogenous source hydrolyzate adds the factors stimulated growth thing, access intestinal bacteria K011, and 37 ℃ are carried out half fermenting cellulosic ethanol.
7. according to claim 1 or 6 described fermentation techniques, the bacterial strain of fermentation hemicellulose hydrolysate is transgenosis intestinal bacteria K011 (ATCC55124).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100577404A CN103146765A (en) | 2013-02-25 | 2013-02-25 | Alcohol from maple hemicellulose and preparation method of alcohol |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100577404A CN103146765A (en) | 2013-02-25 | 2013-02-25 | Alcohol from maple hemicellulose and preparation method of alcohol |
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| CN103146765A true CN103146765A (en) | 2013-06-12 |
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| CN2013100577404A Pending CN103146765A (en) | 2013-02-25 | 2013-02-25 | Alcohol from maple hemicellulose and preparation method of alcohol |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008131229A1 (en) * | 2007-04-19 | 2008-10-30 | Mascoma Corporation | Combined thermochemical pretreatment and refining of lignocellulosic biomass |
| CN101475976A (en) * | 2008-12-30 | 2009-07-08 | 中南林业科技大学 | Multi-strain combined fermentation process for preparing fuel ethanol from wood fiber |
| CN101928733A (en) * | 2009-11-05 | 2010-12-29 | 王建设 | Method and device for producing biological butanol with straw-like materials or agricultural and forestry wastes |
| US20120270289A1 (en) * | 2011-04-19 | 2012-10-25 | U.S. Department Of Agriculture | Co-fermentation of glucose, xylose and/or cellobiose by yeast |
-
2013
- 2013-02-25 CN CN2013100577404A patent/CN103146765A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008131229A1 (en) * | 2007-04-19 | 2008-10-30 | Mascoma Corporation | Combined thermochemical pretreatment and refining of lignocellulosic biomass |
| CN101475976A (en) * | 2008-12-30 | 2009-07-08 | 中南林业科技大学 | Multi-strain combined fermentation process for preparing fuel ethanol from wood fiber |
| CN101928733A (en) * | 2009-11-05 | 2010-12-29 | 王建设 | Method and device for producing biological butanol with straw-like materials or agricultural and forestry wastes |
| US20120270289A1 (en) * | 2011-04-19 | 2012-10-25 | U.S. Department Of Agriculture | Co-fermentation of glucose, xylose and/or cellobiose by yeast |
Non-Patent Citations (1)
| Title |
|---|
| 于泳等: "《柳树稷鲜样及干样的稀酸预处理及乙醇发酵研究》", 《太阳能学报》 * |
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Application publication date: 20130612 |