CN103146699A - Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237 - Google Patents
Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237 Download PDFInfo
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Abstract
The invention belongs to the field of breeding of plants and discloses a molecular marker MAG7237 of a wheat fusarium head blight infection resistant gene Fhb4 and application of the molecular marker MAG7237. The genetic distance between the molecular marker MAG7237 and the Fhb4 gene is 0.34 cm. The molecular marker most closely linked with the Fhb4 is acquired by the invention for the first time in the world. The molecular marker is a co-dominant marker with the advantages of being convenient to detect, and stable and simple to amplify; by adopting the marker MAG7237 to detect the Fhb4 gene, the existence of the Fhb4 or not and the existence state can be determined, and the fusarium head blight resistance of the wheat can be forecasted; and furthermore, the plants with the Fhb4 can be fast screened and used for breeding of disease-resistant varieties.
Description
The application is that the name of on March 27th, 2012 application is called " molecule marker MAG7237 and application thereof that wheat anti gibberellic disease infects gene Fhb4 ", and application number is 201210082937.9 divides an application.
Technical field
The invention belongs to the breeding of plants field, relate to molecule marker and application thereof that wheat anti gibberellic disease infects gene Fhb4.
Technical background
Wheat is one of most important food crop in the world, and the mankind about 20% energy is provided.Many biologies and abiotic stress directly affect the yield and quality of wheat in Wheat Production.Head blight is exactly most important a kind of biological adverse circumstance wherein, has a strong impact on the quality and yield of wheat.The Breeding and application of disease-resistant variety is to control the most economical effective means of head blight, and the excavation of disease-resistant gene is prerequisite and the basis of breeding for disease resistance, and exploitation and the closely linked molecule marker of disease-resistant gene are used the key of disease-resistant gene especially.
Wheat mainly contains five types (Mesterhazy1995) to the resistance of head blight, wherein anti-ly infects (Type I) and anti-expansion (Type II) (Schroeder and Christensen1963) is topmost two types.Type I resistance reflects that mainly wheat opposing the first of pathogenic bacteria infect, and is the first barrier that wheat is resisted gibberella harm, plays vital effect in the resistance reaction.
Manyly studies show that wheat is typical quantitative character to the resistance of head blight, is subjected to controlled by multiple genes.Located up to now more than 200 anti gibberellic disease QTL, wherein be arranged in anti gibberellic disease on 4B karyomit(e) and infect QTL and be present in a plurality of resistance germplasms, express stable and effect stronger.Lin et al(2006) utilize large 2419 recombinant inbred lines in Wangshuibai * south to detect one on the 4B of Wangshuibai karyomit(e) and anti-infect main effect QTL, the phenotypic variation of soluble 15% left and right, and in each time experiment the LOD value all more than 3.Xue et al.(2010a) utilize near isogenic line to confirm the effect of this QTL.Xue et al.(2010b) utilize recombinant inbred lines and near isogenic line colony, method by the screening and identification recombinant chou is interval to the Xhbg226 – Xgwm149 at a distance of 1.7cM with 4B QTL Fine Mapping, being positioned at the wheat disappearance is 4BL5-0.86 – 1.00, and with its called after Fhb4.And there are some researches show that this gene is dominant gene.
Because wheat scab resistance has typical quantitative character feature, be subjected to controlled by multiple genes, easily affected by environment, early generation is difficult to it is selected.In addition, the economical character of many anti gibberellic disease germplasms is relatively poor, and regional adaptability is stronger, utilizes traditional breeding way cultivation resistant variety to waste time and energy and may also can not get expected result.The molecular marker assisted selection breeding is compared with traditional breeding method, can determine from generation to generation in early days the target gene type, and select not to be subjected to the impact of genetic expression and envrionment conditions, can improve the accuracy of selection, thereby accelerate breeding process (Collard and Mackill2008).Although existing research and utilization Molecular Marker Assisted Selection Technology seed selection at present the near isogenic line of Fhb4, the Fhb4 near isogenic line plant height of institute's seed selection is higher, this is disadvantageous selection (Xue et al.2010a) in breeding.Linkage drag when therefore more the exploitation of compact linkage molecule mark is conducive to reduce selection with it improves the application efficiency to Fhb4.In addition, although Fhb4 by Fine Mapping, the genetic distance between the location is still very large, is necessary that the more closely linked molecule marker of exploitation accelerates the research of this gene cloning.
Summary of the invention
The objective of the invention is the above-mentioned deficiency for prior art, provide with wheat anti gibberellic disease and infect the more closely linked molecule marker of gene Fhb4.
Another object of the present invention is to provide the application that this wheat anti gibberellic disease infects the molecule marker of gene Fhb4.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
Wheat anti gibberellic disease infects the molecule marker of gene Fhb4, with labeled primer MAG5184-F:SEQ ID NO.1, MAG5184-R:SEQ ID NO.2 amplification wheat breed genomic dna, the amplified fragments that obtains is 241bp, be wheat anti gibberellic disease infect gene Fhb4 chain molecule marker MAG5184, this is labeled as the codominant marker, and with the Fhb4 close linkage, the genetic distance that utilizes Mapmaker Macintosh V3.0 to record this mark and Fhb4 gene is 0.72cM;
Or with labeled primer MAG7237-F:SEQ ID NO.3, MAG7237-R:SEQ ID NO.4 amplification wheat breed DNA, the amplified fragments that obtains is 688bp, be wheat anti gibberellic disease and infect the chain molecule marker MAG7237 of gene Fhb4, this this be marked in anti gibberellic disease kind Wangshuibai and sense head blight kind Mianyang 99-323 with the HinfI enzyme cut digest exist polymorphic, this is labeled as codominant marker, with the Fhb4 close linkage, the genetic distance that utilizes Mapmaker Macintosh V3.0 to record this mark and Fhb4 gene is 0.34cM;
Or with labeled primer MAG2521-F:SEQ ID NO.5, MAG2521-R:SEQ ID NO.6 amplification wheat breed DNA, the amplified fragments that obtains is 161bp, be wheat anti gibberellic disease and infect the chain molecule marker MAG2521 of gene Fhb4, this is labeled as codominant marker, with the Fhb4 close linkage, the genetic distance that utilizes Mapmaker Macintosh V3.0 to record this mark and Fhb4 gene is 0.92cM.
Described molecule marker anti gibberellic disease in Wheat Germplasm Resources infects the application in the evaluation of gene Fhb4.
wheat anti gibberellic disease infects the molecule marking method of gene Fhb4, with above-mentioned any a pair of molecule marker primer PCR amplification wheat cdna group DNA to be checked, and detection amplified production, if the amplified fragments that can amplify 241bp with primer MAG5184-F and the MAG5184-R of molecule marker MAG5184, the amplified fragments that perhaps amplifies 688bp with primer MAG7237-F and the MAG7237-R of molecule marker MAG7237, the amplified fragments that perhaps can amplify 161bp with primer MAG2521-F and the MAG2521-R of molecule marker MAG2521, indicate that wheat to be checked exists anti gibberellic disease to infect gene Fhb4.
The application of molecule marker of the present invention in the screening wheat-resistance to scab.
utilize the method for above-mentioned molecular marker screening wheat-resistance to scab to be: with above-mentioned any a pair of molecule marker primer PCR amplification wheat cdna group DNA to be checked, and detection amplified production, if the amplified fragments that can amplify 241bp with primer MAG5184-F and the MAG5184-R of molecule marker MAG5184, the amplified fragments that perhaps amplifies 688bp with primer MAG7237-F and the MAG7237-R of molecule marker MAG7237, the amplified fragments that perhaps can amplify 161bp with primer MAG2521-F and the MAG2521-R of molecule marker MAG2521, indicate that wheat to be checked is to exist anti gibberellic disease to infect the wheat-resistance to scab of gene Fhb4.
The application of the primer of described molecule marker in clone's anti gibberellic disease infects gene Fhb4.
The molecule marker that above-mentioned wheat anti gibberellic disease infects gene Fhb4 obtains by the following method:
(1) Wangshuibai Fhb4 near isogenic line NMAS007 and its recurrent parent Mianyang 99-323F
2:3The screening of the establishment of colony and Fhb4 section recombinant chou:
(1) NMAS007(♀) with breed of wheat Mianyang 99-323
Hybridize and obtain hybrid F
1, F
1Selfing produces F
2Colony;
(2) utilize the boundary marker screening F of Fhb4
2The heterozygosis individual plant of restructuring occurs at this section in colony, utilizes same tag at its F
3The individual plant that isozygotys of restructuring occurs in the generation screening at this section.
(2) evaluation of the disease-resistant phenotype of recombinant chou
(3) recombinant chou is bloomed and is broadcasted sowing susceptible wheat inoculation about front ten days in the field, repeats once after the week.Inoculate 15 days sick small ear rates of " Invest, Then Investigate " and estimate its anti-infectivity;
(3) molecular marker analysis
(4) extract disease-resistant parent NMAS007, Susceptible parent Mianyang 99-323 and F with the SDS method
2The DNA of each individual plant of colony; With 27 pairs of SSR primers (http://wheat.pw.usda.gov/GG2/index.shtml) with come from 59 couples of molecule marker primer pair NMAS007 of Wangshuibai BAC clone, Mianyang 99-323, China spring and China spring disappearance is 4BL-5(Endo and Gill1996) carry out polymorphism analysis and specificity analyses;
(5) be chosen in amplify polymorphic between the parent and can deletion mapping to the molecule marker of 4BL5-0.86 – 1.00bin at F
2Obtain the genotype data of each individual plant of colony for amplification in colony's individual plant, and utilize these marker detection heterozygosis recombinant chous offspring F
3The genotype of each individual plant of family;
Wherein, the PCR reaction system is 12.5 μ l, 10 * buffer1.25 μ l wherein, 25mM MgCl
20.75 μ l, 2.5mMdNTPs1 μ l, each 0.2 μ M of left and right primer, Taq enzyme (5u/ μ l) 0.1 μ l, template DNA 10ng adds water to 12.5 μ l;
After the pcr amplification program is 94 ℃ of denaturation 3min, 94 ℃ of sex change 30sec, 60 ℃ of annealing 1min, 72 ℃ are extended 1min, circulate 35 times, and last 72 ℃ are extended 8min; In the enterprising performing PCR amplification of PE9600 amplification instrument, amplified production carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, then take a picture on ultraviolet transilluminator, records result.
(4) molecule marker obtains
(6) according to chain exchange rule, in conjunction with F
2For each individual plant genotype data of colony and heterozygosis recombinant chou F
3The disease-resistant phenotype in the field of family utilizes software Mapmaker Macintosh V3.0 to build the genetic linkage map of Wangshuibai Fhb4, has obtained and the most closely linked molecule marker MAG5184 of Fhb4, MAG7237 and MAG2521.
Beneficial effect:
The present invention has obtained and the closely linked molecule marker MAG5184 of Fhb4, MAG7237 and MAG2521 in the world first.Can accelerate the application of disease-resistant gene Fhb4 in wheat breeding for disease resistance, close and can also be used for the Fhb4 gene cloning.
1, obtained and the closely linked molecule marker MAG5184 of Fhb4, MAG7237 and MAG2521.Chain with Fhb4 in known molecule marker is MAG7237 the most closely, and genetic distance is only 0.34cM, can help this gene to shift in the commercial variety and with the polymerization of other disease-resistant gene.
2, identify conveniently.These three molecule markers are all the codominant marker, have the advantages such as easy to detect, that amplification is stable, easy.Detect the Fhb4 gene with mark MAG7237, the existence that can determine Fhb4 whether and existence, and the scab resistance of prediction wheat, and then rapid screening carries the plant of Fhb4 and is used for the seed selection of disease-resistant variety.Utilizing simultaneously molecule marker to carry out the laboratory detection can avoid environment on the impact of kind.
3, improve the selection determination rates of disease-resistant variety, save cost.In traditional anti gibberellic disease breeding process, the parent and the Cultivar that need to carry disease-resistant gene carry out a series of hybridization, the progeny population individual plant is carried out Disease Resistance Identification, and need the phenotypic evaluation of multiple years; And disease-resistant phenotypic evaluation is subject to the impact of environment, and the phenotypic evaluation result has certain error.Therefore the seed selection of disease-resistant variety is not only time-consuming, and difficulty is large, and cost is high.Infect the closely linked molecule marker of gene Fhb4 by detecting with anti gibberellic disease, the work of phenotypic evaluation can be greatly reduced, and just can identify the individual plant that carries disease-resistant gene Fhb4 in seedling stage, thereby eliminate non-target plant.Therefore, utilize by detecting with anti gibberellic disease and infect the closely linked molecule marker MAG7237 of gene Fhb4, not only save the breeding cost, and greatly improve the efficiency of selection of disease-resistant variety.
4, reduce Linkage drag.Because the scab resistance of wheat often has certain relevant with some bad economical characters.By the higher plant height of the total performance of the anti gibberellic disease strain that carries Fhb4 of molecular selection, this may cause and select the interval of importing larger because the interval of QTL Primary Location is larger, has caused some Linkage drags in early days.Utilize and to infect the closely linked molecule marker of gene Fhb4 with anti gibberellic disease and can reduce Linkage drag when selecting, improve efficiency of selection.
5, can be used for cloning anti gibberellic disease and infect gene Fhb4 research.The prerequisite that the map based cloning anti gibberellic disease infects gene Fhb4 is to obtain and the closely linked molecule marker of Fhb4.MAG5184 and MAG7237 are chain the tightst with Fhb4 in all known molecular marks.For infecting gene Fhb4, clone's anti gibberellic disease provides the basis.
Description of drawings
The isozygoty distribution plan of the sick small ear rate of recombinant chou of 9 of Figure 145.
Fig. 2 MAG5184, MAG7237 and MAG2521 and wheat anti gibberellic disease infect the genetic linkage map of gene Fhb4, and the right is the mark of genetic linkage map, and left data is the genetic distance between mark.
Fig. 3 MAG5184 banding pattern that increases.M is PUC19/MspI, and the left side is molecular weight marker stripe size (bp).1 is Mianyang 99-323,2 is NMAS007,3,5,8,10,11,16,18,19 is susceptible genotype individual plant, and 6,7,22,23,24,26 is disease-resistant gene type individual plant, and 4,9,12,13,14,15,17,20,21,25 is heterozygous genes type individual plant.The arrow indication is the specific amplified band.
Fig. 4 MAG7237 banding pattern that increases.M is D2000, and the left side is molecular weight marker stripe size (bp).1 is NMAS007,2 is Mianyang 99-323,3,6,8,9,11,12,16 is susceptible genotype individual plant, and 5,17,18,19,20,22,23 is disease-resistant gene type individual plant, and 4,7,10,13,14,15,21,24,25,26 is heterozygous genes type individual plant.The arrow indication is the specific amplified band.
Fig. 5 MAG2521 banding pattern that increases.M is PUC19/MspI, and the left side is molecular weight marker stripe size (bp).1 is NMAS007, and 2 is Mianyang 99-323, and 2,3,4,5,10,11,14 is susceptible genotype individual plant, and all the other are heterozygous genes type individual plant.The arrow indication is the specific amplified band.
Embodiment
The molecule marker that wheat anti gibberellic disease infects gene Fhb4 obtains by the following method:
(1) Wangshuibai Fhb4 near isogenic line NMAS007 and its recurrent parent Mianyang 99-323F
2:3The screening of the establishment of colony and Fhb4 section recombinant chou:
(1) NMAS007(♀) with breed of wheat Mianyang 99-323
Hybridize and obtain hybrid F
1, F
1Selfing produces the F that includes 4303 individual plants
2Colony;
(2) utilize boundary marker Xhbg226 and the Xgwm149(Torada et al.2006 of Fhb4;
Et al.1998) at F
2Be sieved to 187 heterozygosis individual plants that restructuring occurs at this section in colony, utilize same tag at its F
3The generation screening obtains 459 individual plants that isozygoty that restructuring occurs at this section.
(2) evaluation of the disease-resistant phenotype of recombinant chou
Recombinant chou is bloomed and is broadcasted sowing susceptible wheat about front ten days in the field to these recombinant chou inoculations of isozygotying, and repeats once after the week.Inoculate 15 days sick small ear rates of " Invest, Then Investigate ".Qualification result shows: these recombinant chou resistances are separated obviously (Fig. 1), and the strain that carries the Fhb4 gene is compared with Susceptible parent, resistance be significantly increased (table 1).
(3) screening of polymorphic molecular marker and recombinant gene type analysis
(1) at first utilize 59 molecule markers of 27 SSR marks (http://wheat.pw.usda.gov/GG2/index.shtml) of being positioned at the Fhb4 section in other collection of illustrative plates and Wangshuibai BAC cloned sequence exploitation to carry out the polymorphism screening to Fhb4 near isogenic line and Mianyang 99-323, finally obtaining 2 is having polymorphic SSR mark and 1 that polymorphic STS mark is arranged between the parent between the parent, i.e. these three molecule markers of MAG5184, MAG2521 and MAG7237.
The PCR reaction system is 12.5 μ l, 10 * buffer1.25 μ l wherein, 25mM MgCl
20.75 μ l, 2.5mM dNTPs1 μ l, each 0.2 μ M of left and right primer, Taq enzyme (5u/ μ l) 0.1 μ l, template DNA 10ng adds water to 12.5 μ l;
After the pcr amplification program is 94 ℃ of denaturation 3min, 94 ℃ of sex change 30sec, 60 ℃ of annealing 1min, 72 ℃ are extended 1min, circulate 35 times, and last 72 ℃ are extended 8min; In the enterprising performing PCR amplification of PE9600 amplification instrument, amplified production carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, then take a picture on ultraviolet transilluminator, records result.
(3) utilization has polymorphic molecule marker MAG5184, MAG7237 and MAG2521 to analyze all recombinant chous that isozygoty between the parent, and these recombinant chous can be divided into 4 kinds of recombinant types (table 1).
(4) acquisition of molecule marker
According to chain exchange rule, in conjunction with F
2For each individual plant genotype data of colony and heterozygosis recombinant chou F
3The disease-resistant phenotype in the field of family (table 1), utilize software Mapmaker Macintosh V3.0 to build the genetic linkage map (Fig. 2) of Wangshuibai Fhb4, obtained and the closely linked molecule marker MAG5184 of Fhb4, MAG7237 and MAG2521, they are respectively apart from Fhb4 gene 0.72cM, 0.34cM, 0.92cM.MAG5184, MAG7237 and MAG2521 molecule marker primer amplification banding pattern are seen Fig. 3, Fig. 4 and Fig. 5.
Table 1NMAS007 – Mianyang 99-323F
2:3Isozygoty in colony genotype and the phenotype of recombinant chou
W represents the Wangshuibai genotype, and M represents Mianyang 99-323 genotype, and C1 represents susceptible contrast Mianyang 99-323, and C2 represents disease-resistant contrast NMAS007.
*Representative is remarkable with the phenotypic difference of disease-resistant contrast NMAS007 on the P=0.01 level.
Claims (6)
1. wheat anti gibberellic disease infects the molecule marker MAG7237 of gene Fhb4, it is characterized in that:
With labeled primer MAG7237-F:SEQ ID NO.3, MAG7237-R:SEQ ID NO.4 amplification wheat breed DNA, the amplified fragments that obtains is 688bp, be wheat anti gibberellic disease and infect the chain molecule marker MAG7237 of gene Fhb4, this is labeled as codominant marker, with the Fhb4 close linkage, the genetic distance that utilizes Mapmaker Macintosh V3.0 to record this mark and Fhb4 gene is 0.34cM.
2. molecule marker claimed in claim 1 anti gibberellic disease in Wheat Germplasm Resources infects the application in the evaluation of gene Fhb4.
3. wheat anti gibberellic disease infects the molecule marking method of gene Fhb4, it is characterized in that with labeled primer MAG7237-F/MAG7237-R pcr amplification claimed in claim 1 wheat cdna group to be checked DNA, and detection amplified production, if amplify the amplified fragments of 688bp, indicate that wheat to be checked exists anti gibberellic disease to infect gene Fhb4.
4. the application of molecule marker claimed in claim 1 in the screening wheat-resistance to scab.
5. application according to claim 4, it is characterized in that utilizing molecule marker claimed in claim 1 in the method for screening wheat-resistance to scab to be: the amplified fragments with primer MAG7237-F and the MAG7237-R of molecule marker MAG7237 claimed in claim 1 amplifies 688bp indicates that wheat to be checked is to exist anti gibberellic disease to infect the wheat-resistance to scab of gene Fhb4.
6. the application of the primer of molecule marker claimed in claim 1 in clone's anti gibberellic disease infects gene Fhb4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104628838A (en) * | 2015-02-03 | 2015-05-20 | 南京农业大学 | Wheat hydrolysis hydroxy cinnamoyl coenzyme A ester protein TaMAG1166 as well as coding gene and application thereof |
| CN110093435A (en) * | 2015-09-28 | 2019-08-06 | 郑州大学 | Wheat SSR molecular labeling primer and its screening technique |
| CN115927698A (en) * | 2022-07-15 | 2023-04-07 | 江苏省农业科学院 | Multiple PCR (polymerase chain reaction) marker primer group for simultaneously detecting wheat scab resistant genes Fhb1 and Fhb7 and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1462808A (en) * | 2003-06-23 | 2003-12-24 | 南京农业大学 | Molecule marker method of wheat Wangshuibai scab resistant major gene locus |
| CN1462806A (en) * | 2003-06-23 | 2003-12-24 | 南京农业大学 | Molecule marker method of wheat Nanda 2419 scab resistant major gene locus |
-
2012
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1462808A (en) * | 2003-06-23 | 2003-12-24 | 南京农业大学 | Molecule marker method of wheat Wangshuibai scab resistant major gene locus |
| CN1462806A (en) * | 2003-06-23 | 2003-12-24 | 南京农业大学 | Molecule marker method of wheat Nanda 2419 scab resistant major gene locus |
Non-Patent Citations (2)
| Title |
|---|
| SHULIN XUE等: "Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.)", 《THEOR APPL GENET》 * |
| 余桂红: "小麦赤霉病抗性遗传分析即分子标记的开发", 《中国博士学位论文全文数据库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104628838A (en) * | 2015-02-03 | 2015-05-20 | 南京农业大学 | Wheat hydrolysis hydroxy cinnamoyl coenzyme A ester protein TaMAG1166 as well as coding gene and application thereof |
| CN110093435A (en) * | 2015-09-28 | 2019-08-06 | 郑州大学 | Wheat SSR molecular labeling primer and its screening technique |
| CN115927698A (en) * | 2022-07-15 | 2023-04-07 | 江苏省农业科学院 | Multiple PCR (polymerase chain reaction) marker primer group for simultaneously detecting wheat scab resistant genes Fhb1 and Fhb7 and application thereof |
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