CN103146607A - Preparation method of active microbial preparation of lactobacillus casei KL1 of produced bile salt hydrolase - Google Patents
Preparation method of active microbial preparation of lactobacillus casei KL1 of produced bile salt hydrolase Download PDFInfo
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Abstract
The invention relates to a preparation method of an active microbial preparation of lactobacillus casei KL1 of produced bile salt hydrolase, which is applicable to the production of microecological preparations such as feeding functional active microbial preparations in the poultry farming industry. According to the invention, the active microbial preparation is prepared by utilizing lactobacillus casei KL1 strains selected from produced bile salt hydrolase in Tibetan kefir, carrying out activation, enlarged cultivation, high-density fermentation and centrifugal concentration, adding a freeze-drying protective agent, freezing in advance, carrying out freeze drying and the like. According to the invention, the fermentation conditions are optimized by using a fully automatic fermentation tank, and the optimal combination formula of the freeze-drying protective additive is determined by taking potato pulp as a freeze-drying protective matrix, so that the active microbial number of the KL1 strains can be more than 1010 CFU/g, and the survival rate can be more than 85%. The active microbial preparation prepared by using the method disclosed by the invention is low in price and high in active microbial content, can be used as a poultry feed additive, and has the characteristics of rapid chicken growth speed, less feed consumption, and few illnesses and the like. The active microbial preparation not only has an effect of reducing cholesterol in egg, but also has a function of adjusting the balancing of intestinal floras of chickens, and has a better prevention and treatment effect on pullorum diseases.
Description
Technical field
The present invention relates to produce the preparation method of bile salt hydrolase lactobacterium casei KL1 active bacteria formulation, be applicable to the production of the probiotics such as feeding use functional active bacteria formulation in poultry livestock industry.
Background technology
The lactobacterium casei that the present invention relates to (Lactobacillus casei) KL1 is separation and purification a kind of milk-acid bacteria with the effect of reduction cholesterol out from the Kai Feier grain.
(Tibetan Kefir, TK) claims again Kai Feier grain, 19th century to be found by the mankind " to hide clever mushroom ".The Kai Feier grain originates from area, Russian Ciscaucasia, local herdsman produces kephir with cow's milk or goat dairy injection sheepskin pocket through spontaneous fermentation, its residue adds cow's milk again or goat dairy continues fermentation, form irregular particle shape object after long-term fermentation, be the Kai Feier grain in the skin pocket.After this people just directly add the Kai Feier grain in cow's milk to, make it to be fermented into the nourishing drink with frank tart flavour and whipability, are Kai Feier.Kai Feier has various health care functions.Viable bacteria in Kai Feier has the strongly inhibited effect to the growth of the pathogenic bacterias such as Shigellae, Salmonellas; Yeast wherein has stronger anti-microbial activity to tubercule bacillus, intestinal bacteria.Therefore Kai Feier has good curative effect to intestinal tract disease, pulmonary tuberculosis.In addition, the meta-bolites that milk-acid bacteria in Kai Feier produces is as the effectively generation of prophylaxis of tumours and hyperlipidemia such as exocellular polysaccharide, bile salt hydrolase.
Microflora is mainly take milk-acid bacteria, yeast and a small amount of acetic bacteria little ecosystem as the basis in the mucopolysaccharide matrix of hiding clever mushroom.The present invention lactobacterium casei KL1 used is separation and purification a kind of probiotic lactobacillus out from hide clever mushroom, can produce the bile salt hydrolase of a large amount of high vigor in the growth metabolism process, through external Oxford cup test and experimentation on animals proof, the effect that reduces body serum total cholesterol content be arranged.It reduces cholesterol mechanism is the bile salt hydrolase (Bile Salt Hydrolase, BSH) that produces because of lactobacterium casei KL1 metabolism.BSH is the meta-bolites that the multiple lactic-acid-bacterium in genus bifidobacterium, lactobacillus, lactococcus in enteron aisle produces in process of growth.Experimental results demonstrate, bile salt hydrolase can decompose in connection with cholate in the liver sausage circulation, produces the lower free cholate of amino acid and solubleness.The latter can be combined with cholesterol the formation sediment composite and be excreted, thereby reduces the content of serum total cholesterol.
Just confirm that absorbing the sour milk that contains certain Bacterium lacticum or bifidobacterium fermentation has the effect that reduces serum cholesterol content as far back as people in 1963.Up to now, Chinese scholars remains in different viewpoints milk-acid bacteria decreasing cholesterol Research on the effect mechanism, mainly concentrates on following 3 points: the direct assimilation cholesterol of (1) milk-acid bacteria somatic cells; (2) the bile salt hydrolase activity of milk-acid bacteria makes the combined cholate be degraded to the free state cholate, and latter's solubleness descends and cholesterol generation coprecipitation effect; (3) other theories are as assimilation and co-precipitation combined action.
At present, the various active bacteria formulation preparation methods' of domestic application patent is more, is (CN101032527) the dual-purpose active bacteria formulation of people beast by the Butylic acid bacteria preparation as Jiangsu Institute of Microbiology Co., Ltd Tang Baoying and Zhu Xiaohui application " active clostridium butyrium agent and preparation method thereof "; " Bifid Triple Viable and the preparation method " of the Chen Bin of Shanghai Xinyi Pharmaceutical Co., Ltd China and Yao Yiqiang application (CN98110623.4) is comprised of as active ingredient and protective material bifidumbacterium bifidum, Lactobacillus acidophilus and streptococcus faecium lyophilize viable bacteria powder; " a kind of composite microorganism viable bacteria preparation and its preparation method and application " of the Zhang Qin of Shanghai Eco-well Bioscience Co., Ltd. and old Lu of building application is (ZL200810034746) to utilize sheet coccus, yeast and genus bacillus fluid enlargement culture respectively; then mix a kind of composite microorganism viable bacteria preparation that makes through solid state fermentation, drying, pulverizing in required ratio.But about utilizing separation screening to prepare active bacteria formulation from the lactobacterium casei KL1 that hides the product bile salt hydrolase in clever mushroom, utilize the Fermentation high density fermentation, and the method for utilizing the potato powder slurry to preserve viable bacteria in biotechnological formulation there is not yet domestic and international pertinent literature report and patent report.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing the lactobacterium casei KL1 bacterial classification that produces bile salt hydrolase to prepare active bacteria formulation.
The present invention is achieved by the following technical solutions:
In described method, lactobacterium casei (Lactobacillus casei) KL1 separation screening from Jilin, the Tibetan of Huhehaote City's average family spirit mushroom (claiming again the Kai Feier grain), and being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 11st, 2006, its preserving number is: CGMCC No.1809 (bacterial strain KL1).Containing on the MRS selective medium of calcium carbonate and tennecetin the bacterium colony size 2～3mm of lactobacterium casei (Lactobacillus casei) KL1 bacterial strain, surface irregularity such as flakes, the edge is irregular, and tarnish is flat, canescence, translucent, bacterium colony has than high viscosity; It is shaft-like that individual morphology is, varying length, and the short chain shape is arranged, and thalline is spherical in shape sometimes, G
+Sporeless bacterium.
In described method, the lactobacterium casei KL1 bacterial strain of skimming milk test tube conservation is cultivated 12h with fresh sterilization skimming milk in 37 ℃, solidify to skimming milk, after so activating for 2～3 generations, refrigerate standby.
In described method, on the basis of above-mentioned test, the bacterium liquid that activation is good to modified MRS culture medium, is cultivated 12hs in 37 ℃ with the inoculum size culture transferring of 2% (V/V, volume fraction), obtains scale-up medium.
In described method, on above-mentioned test-results basis, adopt Three factors-levels optimization of orthogonal test fermentation condition.The 2L modified MRS culture medium is joined in the 5L automatic fermenter, utilize the fermentor tank sterilising system in 115 ℃ of sterilization 15min, utilize cooling-water machine that the substratum in fermentor tank is cooled to leavening temperature.The scale-up medium of KL1 bacterial strain is pressed 3% (V/V, volume fraction) inoculum size culture transferring is to fermentor tank, utilizing the fermentor tank automatic temperature control system to keep leavening temperature is 40 ℃, adds the sterilization Na of 10% (W/V, mass volume ratio concentration) in fermenting process with stream
2CO
3Solution controlled fermentation liquid pH6.4, mixing speed is 55r/min, fermentation 20h obtains fermented liquid.
In aforesaid method, the modified MRS culture medium composition: glucose 10g, soy peptone 10g, extractum carnis 10g, yeast dry powder 5g, dibasic ammonium citrate 2g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, bitter salt 0.58g, four anhydrous manganese 0.25g, tween-80 1mL, distilled water 1000mL, regulating pH is 6.4.
In aforesaid method, the 5L fermentor tank is produced by Shanghai high machine biotechnology company limited, and its model is BIOF6005GBN.
In described method, on above-mentioned test-results basis, under 4 ℃ of conditions, the centrifugal 15min of 5000r/min abandons supernatant liquor with the 2L fermented liquid, collects and obtains bacterium mud.
In described method; on above-mentioned test-results basis; the bacterium mud of collecting is on average added in 2 600mL freeze-drying bottles that fill 100mL potato powder slurry (frozen-dried protective matrix); add 3.5% Sodium Glutamate, 2.0% skim-milk and 1.5% glycerine as lyophilized vaccine; after mixing, obtain concentrated active bacteria formulation.
In aforesaid method; adopt single factor multilevel test; the more excellent combination formula of lyophilized vaccine of determining lactobacterium casei KL1 is (W/V, mass volume ratio concentration): 3.5% Sodium Glutamate+2.0% skim-milk (Erie)+1.5% glycerine (V/V, volume fraction).
In aforesaid method, fresh peeling potato with nine positive board food cooking machine making beating, is namely become the potato powder slurry.
In described method, will concentrate active bacteria formulation in-34 ℃ of pre-freeze 15h to the fully charge state, obtain the pre-freeze active bacteria formulation.
In described method, on above-mentioned test-results basis, utilize 6L LABCONCO vacuum freeze drier (U.S.) with the pre-freeze active bacteria formulation under the condition of freeze temperature-55 ℃, vacuum tightness 0.16mBar, freeze-drying 48h obtains the freeze drying viable microorganism preparation to the complete drying state.
In described method, the number of viable that gained produces bile salt hydrolase lactobacterium casei KL1 active bacteria formulation can reach 10
10More than CFU/g, the bacterial classification survival rate can reach more than 85%.This kind active bacteria formulation due to potato powder slurry as frozen-dried protective matrix, make product price cheap, viable bacteria content is high, can be used as livestock fodder additives, has that chicken fast growth, feed are low-consuming, few characteristics such as sick.It not only has the effect that reduces egg cholesterol, and has the intestinal microflora poising action of regulating chicken, and white dysentery is had better preventive and therapeutic action.
Product bile salt hydrolase lactobacterium casei KL1 active bacteria formulation that aforesaid method obtains and preparation method thereof belongs to protection domain of the present invention.
Embodiment
The invention will be further described and do not limit protection scope of the present invention for following embodiment.
Experimental technique in following embodiment if no special instructions, is ordinary method.
Percentage composition in following embodiment if no special instructions, is volume fraction.
Embodiment 1, the preparation method who produces bile salt hydrolase lactobacterium casei KL1 active bacteria formulation
1, the activation of bacterial classification and enlarged culturing
The lactobacterium casei KL1 bacterial strain of skimming milk test tube conservation is cultivated 12h with fresh sterilization skimming milk in 37 ℃, solidify to skimming milk, so activated for 2～3 generations, refrigerate standby; The bacterium liquid that activation is good to modified MRS culture medium, is cultivated 12hs in 37 ℃ with 2% inoculum size culture transferring, obtains scale-up medium.
2, fermention medium determines
Prepare respectively 3 kinds of fermention mediums: the milk medium of sterilization, modified MRS culture medium, milk+10% Radix Dauci Sativae substratum.The bacterial classification that activation is good is pressed 2% inoculum size difference culture transferring to above-mentioned substratum, after 37 ℃ of fermentation 12h, adopts the MRS substratum with the number of viable in pour plate culture method detection fermented liquid, and each extent of dilution is established 3 repetitions, the results are shown in Table 1.
The impact of table 1 different fermentations substratum on lactobacterium casei KL1 number of viable
As shown in Table 1, when fermenting with modified MRS culture medium, number of viable is higher, reaches 2.95 * 10
8CFU/mL adopts the number of viable of milk+10% Radix Dauci Sativae substratum to take second place, and is 1.10 * 10
8CFU/mL, and adopt the number of viable of milk medium minimum, therefore determine that fermention medium is modified MRS culture medium.
3, optimization of orthogonal test lactobacterium casei KL1 fermentation condition
(1) orthogonal test
According to single factor multilevel test result, selecting leavening temperature, fermentation time, fermentation inoculum size is influence factor, design Three factors-levels (L
9(3
3)) orthogonal test.In the 5L Fermentation, take the 2L modified MRS as fermention medium, add the sterilization Na of 10% (W/V, mass volume ratio concentration) with stream in the batch fermentation process
2CO
3Solution controlled fermentation liquid pH6.4 adopts the MRS substratum with the number of viable in pour plate culture method detection fermented liquid, and each extent of dilution is established 3 repetitions, the results are shown in Table 2.
Table 2 lactobacterium casei KL1 fermentation condition optimization orthogonal experiments
By table 2 range analysis as can be known, to the order of the viable count influence factor of fermented liquid be: B＞A＞C, namely fermentation time has the greatest impact, and leavening temperature takes second place, and the impact of fermentation inoculum size is minimum; The optimum combination that is drawn the batch fermentation condition by intuitive analysis is A
3B
3C
1, namely optimization of fermentation conditions is: leavening temperature is 40 ℃, and fermentation time is 20h, and inoculum size is 3%.
(2) proof test
Carry out proof test according to the optimization of orthogonal test result.Take the 2L modified MRS as fermention medium, add 10% sterilization Na in the batch fermentation process with stream in the 5L Fermentation
2CO
3Solution controlled fermentation liquid pH6.4, with controlled fermentation liquid pH (nature) not as a control group.Adopt the MRS substratum with the number of viable in pour plate culture method detection fermented liquid, each extent of dilution is established 3 repetitions, the results are shown in Table 3.
The proof test result of table 3 orthogonal test
As shown in Table 3, ferment under optimal conditions, the fermented liquid viable count reaches 8.2 * 10
9CFU/mL is for optimizing front (3.2 * 10
8CFU/mL) 25.63 of viable count times.
4, high density fermentation
The 2L modified MRS culture medium is added in the 5L Fermentation, utilize the fermentor tank sterilising system in 115 ℃ of sterilization 15min, utilize cooling-water machine that the substratum in fermentor tank is cooled to leavening temperature.With the scale-up medium of KL1 bacterial strain by 3% inoculum size culture transferring to fermentor tank, utilizing the fermentor tank temperature controlling system to keep leavening temperature is 40 ℃, adds 10% sterilization Na in fermenting process with stream
2CO
3The pH of solution controlled fermentation liquid is 6.4, and mixing speed is 55r/min, and fermentation 20h obtains viable count 10
9The fermented liquid that CFU/mL is above.
5, centrifugal concentrating and add protective material
Fresh peeling potato with nine positive board food cooking machine making beating, is become the potato powder slurry; By the amount of above-mentioned centrifugal primary fermentation liquid 1/10 volume, get the potato powder slurry as frozen-dried protective matrix, be sub-packed in the freeze-drying bottle of 600mL, the bottled amount of each freeze-drying is 100mL; Get the 1L fermented liquid in 4 ℃, the centrifugal 15min of 5000r/min, abandon supernatant liquor, collect bacterium mud, be added to respectively in frozen-dried protective matrix; Add lyophilized vaccine by table 4 formula, after mixing, obtain concentrated active bacteria formulation.Preparation number of viable before adopting the MRS substratum with pour plate culture method detection freeze-drying, each extent of dilution is established 3 repetitions, the results are shown in Table 4, to determine the more excellent combination formula of lyophilized vaccine.
The impact of the lyophilized vaccine of table 4 various combination on lactobacterium casei KL1 survival rate
By as seen from Table 4, when choosing lyophilized vaccine and being combined as 3.5% Sodium Glutamate+2.0% skim-milk+1.5% glycerine, lactobacterium casei KL1 survival rate is the highest, is 85.43%; During as lyophilized vaccine, the bacterial classification survival rate is taken second place, and is 78.08% take 3.5% Sodium Glutamate+2.0% skim-milk; During take 3.5% Sodium Glutamate as lyophilized vaccine, bacterial classification survival rate minimum is 45.62%.Therefore determine that the more excellent combination formula of lyophilized vaccine of lactobacterium casei KL1 is (W/V, mass volume ratio concentration): 3.5% Sodium Glutamate+2.0% skim-milk+1.5% glycerine (V/V).Its number of viable can reach 10
10More than CFU/g, the bacterial classification survival rate can reach more than 85%.
6, pre-freeze and freeze-drying
To concentrate active bacteria formulation in-34 ℃ of pre-freeze 15h to the fully charge state, obtain the pre-freeze active bacteria formulation.Utilize 6L LABCONCO vacuum freeze drier (U.S.) with the pre-freeze active bacteria formulation under-55 ℃, the condition of vacuum tightness 0.16mBar, freeze-drying 48h obtains the freeze drying viable microorganism preparation to the complete drying state.Preparation number of viable after adopting the MRS substratum with pour plate culture method detection freeze-drying calculates the bacterial classification survival rate, and each extent of dilution is established 3 repetitions, the results are shown in Table 4.
In sum, lactobacterium casei KL1 is cultivated 12h with the sterilization skimming milk in 37 ℃, solidify to skimming milk, so activated for 2～3 generations; The bacterium liquid that activation is good to modified MRS culture medium, is cultivated 12hs in 37 ℃ with 2% inoculum size culture transferring, obtains scale-up medium; With above-mentioned scale-up medium by 3% inoculum size culture transferring to 2L modified MRS fermention medium, utilize the 5L Fermentation to sterilize and high density fermentation, 40 ℃ of controlled fermentation temperature, fermented liquid pH6.4, mixing speed 55r/min, fermentation time 20h obtains fermented liquid; Fermented liquid in 4 ℃, the centrifugal 15min of 5000r/min, is collected and obtained bacterium mud; Press the amount (200mL) of centrifugal primary fermentation liquid 1/10 volume in 600mL freeze-drying bottle, add the potato powder slurry as frozen-dried protective matrix, add again the lyophilized vaccine of 3.5% Sodium Glutamate, 2.0% skim-milk and 1.5% glycerine and the bacterium mud of collection, after mixing, obtain starter culture concentrates; With the bottled amount of each freeze-drying be the starter culture concentrates of 100mL in-34 ℃ of pre-freeze 15h to the fully charge state, obtain the pre-freeze active bacteria formulation; Utilize 6L LABCONCO vacuum freeze drier (U.S.) with the pre-freeze active bacteria formulation under-55 ℃, the condition of vacuum tightness 0.16mBar, freeze-drying 48h obtains the freeze drying viable microorganism preparation to the complete drying state.
The number of viable that gained produces bile salt hydrolase lactobacterium casei KL1 active bacteria formulation can reach 10
10More than CFU/g, the bacterial classification survival rate can reach more than 85%.This active bacteria formulation is cheap, and number of viable is high, can be used as livestock fodder additives, has that chicken fast growth, feed are low-consuming, few characteristics such as sick.It not only has the effect that reduces egg cholesterol, and has the intestinal microflora poising action of regulating chicken, and white dysentery is had better preventive and therapeutic action.
Project 1 under this patent: Beijing's Natural Science Fund In The Light " is hidden the research that clever mushroom probiotic bacterium produces active substances and physiological function "
Item number: 5062006
The project beginning and ending time: 2006.01-2008.12
Project leader: Liu Hui
Claims (1)
1. one kind is utilized bacterial strain KL1CGMCC No.1809 to prepare the method for active bacteria formulation for the product bile salt hydrolase milk-acid bacteria of lactobacterium casei (Lactobacillus casei), it is characterized in that: with lactobacterium casei KL1 scale-up medium by 3% inoculum size culture transferring to 2L modified MRS fermention medium, utilize the 5L automatic fermenter to sterilize and high density fermentation, 40 ℃ of controlled fermentation temperature, fermented liquid pH6.4, mixing speed 55r/min, fermentation time 20h obtains fermented liquid; Fermented liquid in 4 ℃, the centrifugal 15min of 5000r/min, is collected and obtained bacterium mud; Press the amount of centrifugal primary fermentation liquid 1/10 volume in 600mL freeze-drying bottle, add the potato powder slurry as frozen-dried protective matrix, add again the lyophilized vaccine of 3.5% Sodium Glutamate, 2.0% skim-milk and 1.5% glycerine and the bacterium mud of collection, after mixing, obtain starter culture concentrates; With the bottled amount of each freeze-drying be the starter culture concentrates of 100mL in-34 ℃ of pre-freeze 15h to the fully charge state, obtain the pre-freeze active bacteria formulation; Utilize the U.S. produce 6L LABCONCO vacuum freeze drier with the pre-freeze active bacteria formulation under-55 ℃, the condition of vacuum tightness 0.16mBar, freeze-drying 48h obtains lactobacterium casei KL1 freeze drying viable microorganism preparation to the complete drying state.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357359A (en) * | 2014-11-14 | 2015-02-18 | 北京北农汇邦生物科技有限公司 | Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation |
| CN104694415A (en) * | 2014-12-04 | 2015-06-10 | 北京农学院 | Method for manufacturing cholesterol-reducing lactobacillus paracasei probiotic chocolate |
| CN106635886A (en) * | 2016-11-18 | 2017-05-10 | 北京农学院 | Method for reducing egg cholesterol by utilizing lactobacillus paracasei of bile salt producing hydrolase |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331900A (en) * | 2007-06-28 | 2008-12-31 | 北京农学院 | Four kinds of lactobacillus for generating bilesalt hydrolase and extracellular polysaccharide and functionality yoghourt production technique thereof |
| CN101642274A (en) * | 2009-08-21 | 2010-02-10 | 北京农学院 | Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase |
| CN102524794A (en) * | 2012-01-10 | 2012-07-04 | 北京农学院 | Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder |
| CN102586137A (en) * | 2012-01-10 | 2012-07-18 | 北京农学院 | Method for preparing bile salt hydrolase lactobacillus powder starter |
-
2013
- 2013-03-05 CN CN201310068240.0A patent/CN103146607B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331900A (en) * | 2007-06-28 | 2008-12-31 | 北京农学院 | Four kinds of lactobacillus for generating bilesalt hydrolase and extracellular polysaccharide and functionality yoghourt production technique thereof |
| CN101642274A (en) * | 2009-08-21 | 2010-02-10 | 北京农学院 | Method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase |
| CN102524794A (en) * | 2012-01-10 | 2012-07-04 | 北京农学院 | Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder |
| CN102586137A (en) * | 2012-01-10 | 2012-07-18 | 北京农学院 | Method for preparing bile salt hydrolase lactobacillus powder starter |
Non-Patent Citations (2)
| Title |
|---|
| 刘慧 等: "藏灵菇源干酪乳杆菌KL1高产胆盐水解酶发酵条件的优化研究", 《中国农学通报》 * |
| 刘慧 等: "藏灵菇源干酪乳杆菌KL1高产胞外多糖发酵条件的优化研究", 《中国农学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357359A (en) * | 2014-11-14 | 2015-02-18 | 北京北农汇邦生物科技有限公司 | Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation |
| CN104694415A (en) * | 2014-12-04 | 2015-06-10 | 北京农学院 | Method for manufacturing cholesterol-reducing lactobacillus paracasei probiotic chocolate |
| CN106635886A (en) * | 2016-11-18 | 2017-05-10 | 北京农学院 | Method for reducing egg cholesterol by utilizing lactobacillus paracasei of bile salt producing hydrolase |
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