CN103145858B - Method for extracting flavone-rich pueraria starch from pueraria lobata - Google Patents
Method for extracting flavone-rich pueraria starch from pueraria lobata Download PDFInfo
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- CN103145858B CN103145858B CN201310088045.4A CN201310088045A CN103145858B CN 103145858 B CN103145858 B CN 103145858B CN 201310088045 A CN201310088045 A CN 201310088045A CN 103145858 B CN103145858 B CN 103145858B
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Abstract
The invention discloses a method for extracting flavone-rich pueraria starch from pueraria lobata. The method comprises the following steps of: cutting clean and fresh pueraria lobata intio sections; adding color fixing solution; integrally grinding to obtain size; carrying out enzymolysis on the size by cellulose at 48 to 50 DEG C along with pH (Potential Of Hydrogen) of 4.6 to 5.0; dipping the mixture subjected to the enzymolysis in alkaline solution with pH of 8 to 10 for 30 to 60 minutes; filtering to remove the slag to obtain the pueraria starch; intermittently processing the obtained pueraria starch by micro wave with power of 450 to 600w in a way of processing for 10 to 30 seconds each time; adjusting the pueraria starch subjected to microwave processing to reach pH of 5 to 6; centrifugally separating; and drying the solid so as to obtain the flavone-rich pueraria starch. By adopting the method, the nutritive value of the pueraria starch can be effectively increased; and the method is simple, fast in speed and capable of realizing industrial production.
Description
Technical field
The present invention relates to and a kind ofly from the root of kudzu vine, extract the method for the pueraria starch of flavones of being rich in, belong to protective foods preparation field.
Background technology
The root of kudzu vine (Radix Pueraria) is the subterraneous root of Pueraria, for perennial pulse family vine, begin to be loaded in Shennong's Herbal, be listed in middle product, because of rich in starch and flavone component, be classified as " being food and medicine " register by the Ministry of Health.In recent years, domestic and international modern medicine has carried out deep systematic study to the medicinal ingredients of the root of kudzu vine and pharmacological action, and showing that Radix Puerariae flavone has increases the function such as brain and coronary flow, can be used for the treatment of cardio-cerebrovascular diseases, both domestic and external in great demand.
Rich in starch (being commonly called as kudzuvine root starch) in the root of kudzu vine, if the composition that the food transparency made of the root of kudzu vine is high, especially remain Radix Puerariae flavone in synersis and storage in kudzuvine root starch, then than other starch, there is larger superiority, so the root of kudzu vine is excellent healthy food material.
Except containing starch and Radix Puerariae flavone in the root of kudzu vine, in addition also containing the composition such as fiber, protein, the existing root of kudzu vine utilize technique such as kudzuvine root starch produce, Radix Puerariae flavone extracts, generally only make use of the utilization of one-component starch in the root of kudzu vine or flavones, cause the waste of root of kudzu vine resource.
Radix Puerariae flavone is mainly present in the epidermic cell of the root of kudzu vine, therefore, and the cellulosic obstruction of main component of the extraction of Radix Puerariae flavone not only Gonna breakthrough root of kudzu vine epidermis, and to ensure that flavones does not destroy loss, exists technical difficulty simultaneously.Equally, in the process that pueraria starch extracts, root of kudzu vine fiber is also main barrier to the stripping of pueraria starch, becomes the technical barrier extracting high-content Radix Puerariae flavone starch.
Summary of the invention
The utilization of one-component starch or flavones is only paid close attention to when the present invention is directed to the current root of kudzu vine utilization of resources, cause the waste of root of kudzu vine resource, particularly the stripping of cell walls to the flavones in the root of kudzu vine and starch of root of kudzu vine epidermic cell causes the difficult problem in prior art, object is to provide one can effectively improve pueraria starch nutritive value, and simply, rapidly from the root of kudzu vine, high yield extracts the method for pueraria starch and flavones simultaneously.
The invention provides and a kind ofly from the root of kudzu vine, extract the method for the pueraria starch of flavones of being rich in, the method adds colour protecting liquid after first being cut into chunks by the clean fresh root of kudzu vine, obtains slurries together through milling; The slurries cellulase obtained is 48 ~ 50 DEG C in temperature, and pH is 4.6 ~ 5.0 times enzymolysis; The mixture obtained after enzymolysis completes is soak 30 ~ 60min in 8 ~ 10 basic solutions in pH value, then obtains starch milk after elimination slag after filtration; The starch milk obtained under power is the microwave of 450 ~ 600w to process the mode intermittent type process 2 ~ 3 times of 10 ~ 30s at every turn; It is 5 ~ 6 rear centrifugations that starch milk microwave treatment crossed is adjusted to pH, by solid drying, to obtain final product.
Described colour protecting liquid and the mass ratio of the root of kudzu vine are 1:4 ~ 8.
The content of described colour protecting liquid to be the content of Cys be 0.1 ~ 1.0wt% and EDTA is the mixed aqueous solution of 0.1 ~ 0.35wt%.
The concentration of described cellulase is 0.03 ~ 0.055g/100g root of kudzu vine.
The acid adopted pH regulator to 5 ~ 6 in the inventive method is one or more in acetic acid, citric acid or hydrochloric acid.
Described basic solution be in liming or sodium hydroxide solution one or both.
The described root of kudzu vine is cut into every section of 10 ~ 20cm.
The concrete grammar that the present invention extracts the pueraria starch being rich in flavones from the root of kudzu vine comprises the following steps:
1, by fresh root of kudzu vine removal of contamination, clean, segment;
The root of kudzu vine is cut into the section block of 10 ~ 20cm length;
2, the root of kudzu vine of segment is added defibrination after colour protecting liquid, the mass ratio of colour protecting liquid and material is 1:4 ~ 8;
The content of colour protecting liquid to be the content of Cys be 0.1 ~ 1.0wt% and EDTA is the mixed aqueous solution of 0.1 ~ 0.35wt%;
3, the slurries obtained after defibrination are adopted cellulase degradation;
Enzymatic hydrolysis condition: temperature 48 ~ 50 DEG C, the consumption of enzyme is 0.03 ~ 0.055g/100g root of kudzu vine, and enzymolysis time is 2 ~ 3h, pH is 4.6 ~ 5.0;
4, soak 30 ~ 60min after the mixture after enzymolysis being regulated pH to 8 ~ 10 with basic solution, the mixture after immersion is carried out screening and removes slag, obtain pueraria starch milk;
Described basic solution be in liming or sodium hydroxide solution one or both.
5, pueraria starch milk is carried out the microwave treatment of interval;
The power of microwave is 450 ~ 600w, and the time of microwave treatment is 10 ~ 30s, and the number of times of process is 2 ~ 3 times;
It is 5 ~ 6 rear centrifugations that the pueraria starch milk acid of 6, microwave treatment being crossed is adjusted to pH, obtains the wet starch of solid, reaches the pueraria starch finished product being rich in flavones after drying;
The acid adopted is one or more in acetic acid, citric acid or hydrochloric acid.
Beneficial effect of the present invention:
1, by cellulase again in conjunction with microwave action, reach the effect effectively destroying root of kudzu vine epidermic cell wall, make the effective stripping of the flavones in the root of kudzu vine and do not destroy its structure, ensure that other nutritive ingredients such as protein do not run off simultaneously;
2, by the strict control to pH value, Radix Puerariae flavone is retained in pueraria starch in a large number, its content higher than the pueraria starch that traditional technology is produced, fully efficiently utilizes kudzu root nutrient material, improves the nutritive value of product far away;
3, in leaching process, effectively avoiding the brown stain of material by adding colour protecting liquid, ensureing the quality of product;
4, this extracting method is simple, fast, equipment is simple, can suitability for industrialized production.
Embodiment
Below by embodiment, the present invention will be further described, instead of restriction the present invention.
Embodiment 1:
1, the fresh root of kudzu vine is known impurity, clean, segment, length is 10 ~ 20cm;
2, wear into slurries after the root of kudzu vine of segment being added colour protecting liquid, the mass ratio of colour protecting liquid and the root of kudzu vine is 1:5; The content of colour protecting liquid to be the content of Cys be 0.5wt% and EDTA is the mixed aqueous solution of 0.25wt%;
3, slurries are adopted cellulase degradation, enzymatic hydrolysis condition: temperature 50 C, the enzyme concn 0.05g/100g root of kudzu vine, the time is 2.5h, pH is 4.8;
4, soak 60min after the mixture liming after enzymolysis being regulated pH to 9, carry out screening and remove slag, obtain pueraria starch milk;
5, pueraria starch milk is carried out the microwave treatment of interval, the power of microwave is 500w, and the time of microwave treatment is 30s, and the number of times of process is 3 times;
6, microwave treatment pueraria starch milk acetic acid being adjusted to pH is 6 rear centrifugations, reaches the pueraria starch finished product being rich in flavones after the solid-phase drying obtained.
According to above-mentioned technique, the extraction rate reached of final pueraria starch is to 95%, and the extraction rate reached of Radix Puerariae flavone is to 90%.
Embodiment 2:
1, the fresh root of kudzu vine is known impurity, clean, segment, length is 10 ~ 20cm;
2, wear into slurries after the root of kudzu vine of segment being added colour protecting liquid, the mass ratio of colour protecting liquid and the root of kudzu vine is 1:8; The content of colour protecting liquid to be the content of Cys be 0.7wt% and EDTA is the mixed aqueous solution of 0.15wt%;
3, slurries are adopted cellulase degradation, enzymatic hydrolysis condition: temperature 48 DEG C, the enzyme concn 0.055g/100g root of kudzu vine, the time is 2h, pH is 4.8;
4, soak 30min after the mixture liming after enzymolysis being regulated pH to 8, carry out screening and remove slag, obtain pueraria starch milk;
5, pueraria starch milk is carried out the microwave treatment of interval, the power of microwave is 450w, and the time of microwave treatment is 30s, and the number of times of process is 3 times;
6, microwave treatment pueraria starch milk citric acid being adjusted to pH is 5 rear centrifugations, reaches the pueraria starch finished product being rich in flavones after the solid-phase drying obtained.
According to above-mentioned technique, the extraction rate reached of final pueraria starch is to 94%, and the extraction rate reached of Radix Puerariae flavone is to 93%.
Embodiment 3
1, the fresh root of kudzu vine is known impurity, clean, segment, length is 10 ~ 20cm;
2, wear into slurries after the root of kudzu vine of segment being added colour protecting liquid, the mass ratio of colour protecting liquid and the root of kudzu vine is 1:4; The content of colour protecting liquid to be the content of Cys be 0.1wt% and EDTA is the mixed aqueous solution of 0.1wt%;
3, slurries are adopted cellulase degradation, enzymatic hydrolysis condition: temperature 50 C, the enzyme concn 0.055g/100g root of kudzu vine, the time is 2h, pH is 4.8;
4, soak 30min after the mixture sodium hydroxide after enzymolysis being regulated pH to 10, carry out screening and remove slag, obtain pueraria starch milk;
5, pueraria starch milk is carried out the microwave treatment of interval, the power of microwave is 450w, and the time of microwave treatment is 30s, and the number of times of process is 3 times;
6, microwave treatment pueraria starch milk hydrochloric acid being adjusted to pH is 5 rear centrifugations, reaches the pueraria starch finished product being rich in flavones after the solid-phase drying obtained.
According to above-mentioned technique, the extraction rate reached of final pueraria starch is to 94%, and the extraction rate reached of Radix Puerariae flavone is to 95%.
Embodiment 4
1, the fresh root of kudzu vine is known impurity, clean, segment, length is 10 ~ 20cm;
2, wear into slurries after the root of kudzu vine of segment being added colour protecting liquid, the mass ratio of colour protecting liquid and the root of kudzu vine is 1:6; The content of colour protecting liquid to be the content of Cys be 0.1wt% and EDTA is the mixed aqueous solution of 0.35wt%;
3, slurries are adopted cellulase degradation, enzymatic hydrolysis condition: temperature 50 C, the enzyme concn 0.045g/100g root of kudzu vine, the time is 3h, pH is 4.8;
4, soak 60min after the mixture liming after enzymolysis being regulated pH to 10, carry out screening and remove slag, obtain pueraria starch milk;
5, pueraria starch milk is carried out the microwave treatment of interval, the power of microwave is 600w, and the time of microwave treatment is 30s, and the number of times of process is 3 times;
6, microwave treatment pueraria starch milk acetic acid being adjusted to pH is 6 rear centrifugations, reaches the pueraria starch finished product being rich in flavones after the solid-phase drying obtained.
According to above-mentioned technique, the extraction rate reached of final pueraria starch is to 95%, and the extraction rate reached of Radix Puerariae flavone is to 95%.
Claims (4)
1. from the root of kudzu vine, extract the method being rich in the pueraria starch of flavones, it is characterized in that, adding colour protecting liquid after first being cut into chunks by the clean fresh root of kudzu vine, obtaining slurries through milling together; The slurries cellulase obtained is 48 ~ 50 DEG C in temperature, and pH is 4.6 ~ 5.0 times enzymolysis; The mixture obtained after enzymolysis completes is soak 30 ~ 60min in 8 ~ 10 basic solutions in pH value, then obtains pueraria starch milk after elimination slag after filtration; The pueraria starch milk obtained under power is the microwave of 450 ~ 600w to process the mode intermittent type process 2 ~ 3 times of 10 ~ 30s at every turn; It is 5 ~ 6 rear centrifugations that pueraria starch milk microwave treatment crossed is adjusted to pH, by solid drying, to obtain final product; Described colour protecting liquid and the mass ratio of the root of kudzu vine are 1:4 ~ 8; The content of described colour protecting liquid to be the content of Cys be 0.1 ~ 1.0wt% and EDTA is the mixed aqueous solution of 0.1 ~ 0.35wt%.
2. the method for claim 1, is characterized in that, the consumption of described cellulase is 0.03 ~ 0.055g/100g root of kudzu vine.
3. the method for claim 1, is characterized in that, the acid adopted pH regulator to 5 ~ 6 is one or more in acetic acid, citric acid or hydrochloric acid.
4. the method for claim 1, is characterized in that, described basic solution be in liming or sodium hydroxide solution one or both.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554276A (en) * | 2013-10-15 | 2014-02-05 | 温天文 | Technological process for extracting starch, cellulose and general flavone in root of kudzu vine by use of organic membrane |
| CN103766699A (en) * | 2014-01-13 | 2014-05-07 | 凤台县银柳食品有限公司 | Arrowroot, corn and kudzu root starch and preparation method thereof |
| CN103875909B (en) * | 2014-04-08 | 2015-04-29 | 山东金胜粮油集团有限公司 | Technology for extracting flavone of peanut shells |
| CN104041771B (en) * | 2014-06-23 | 2016-05-18 | 曾世均 | A kind of processing method of root of kudzu vine comprehensive utilization |
| CN104522217A (en) * | 2015-01-22 | 2015-04-22 | 重庆中宝生物制药有限公司 | Vine tea extract and applications thereof |
| CN107853635A (en) * | 2017-11-10 | 2018-03-30 | 德兴市宋氏葛业有限公司 | A kind of preparation method of instant modified kudzuvine root starch |
| CN108294261A (en) * | 2018-01-31 | 2018-07-20 | 李玟君 | A kind of kudzu-vine root powder and its processing method rich in resistant starch |
| CN111333737B (en) * | 2020-04-23 | 2022-03-04 | 广西壮族自治区林业科学研究院 | Preparation method of chestnut starch rich in flavone |
| CN114057901B (en) * | 2021-11-26 | 2023-04-25 | 广西壮族自治区中国科学院广西植物研究所 | Method for preparing modified starch by direct dry separation of radix puerariae |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62126185A (en) * | 1985-11-28 | 1987-06-08 | Tsumura Juntendo Inc | Production of isoflavone derivative |
| CN1563095A (en) * | 2004-03-17 | 2005-01-12 | 广东省农业科学院农业生物技术研究所 | Method for extracting starch containing high general flavone from kudzuvine root |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
| CN101736052A (en) * | 2009-12-25 | 2010-06-16 | 重庆正里元实业有限公司 | Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone |
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2013
- 2013-03-19 CN CN201310088045.4A patent/CN103145858B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62126185A (en) * | 1985-11-28 | 1987-06-08 | Tsumura Juntendo Inc | Production of isoflavone derivative |
| CN1563095A (en) * | 2004-03-17 | 2005-01-12 | 广东省农业科学院农业生物技术研究所 | Method for extracting starch containing high general flavone from kudzuvine root |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
| CN101736052A (en) * | 2009-12-25 | 2010-06-16 | 重庆正里元实业有限公司 | Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone |
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