CN103141421B - Character identification method for macrobrachium rosenbergii - Google Patents
Character identification method for macrobrachium rosenbergii Download PDFInfo
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- CN103141421B CN103141421B CN201310079563.XA CN201310079563A CN103141421B CN 103141421 B CN103141421 B CN 103141421B CN 201310079563 A CN201310079563 A CN 201310079563A CN 103141421 B CN103141421 B CN 103141421B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a character identification method for macrobrachium rosenbergii. The method comprises the steps that measuring points are positioned on macrobrachium rosenbergii samples; the samples are classified according to different body lengths and weights; measured values of the samples of the types are measured; an average is worked out; a ratio of the measured values to measured telson data is calculated; a correlation proportion average is obtained; correlation proportion averages of measured data for the types of samples and all the types of samples are subjected to contrastive analysis; and technical operation of macrobrachium rosenbergii germplasm selection is accomplished according to an analysis result. According to the character identification method, the ratio of measuring results of all the type samples to the telson correspondingly varies with different growth types of the macrobrachium rosenbergii, and the value change for a special colony has a relatively obvious difference. Therefore, the character identification method can be applied for selecting and remaining different macrobrachium rosenbergii individuals, special individuals can be found as early as possible, and a success rate of good variety breeding of the macrobrachium rosenbergii is increased.
Description
Technical field
The invention belongs to technical field of aquaculture, especially relate to a kind of proterties discrimination method of Macrobrachium rosenbergii.
Background technology
< < fresh water shrimp-Macrobrachium rosenbergii cultivation handbook > > introduction according to FAO's FAO shared file storehouse, Macrobrachium rosenbergii is transferred to other places, the world from its original habitat, to be the object in order studying at first, as commercial aquaculture kind, by numerous countries, to be introduced afterwards.Nineteen sixty-five this kind after Malaysia is introduced into Hawaii, Ling (1969) the initiative work is there developed into large-scale postlarva production method by Fujimura and Okamoto (1972), and this method is almost adopted by each continent.Present Macrobrachium rosenbergii major country of production is Bangladesh, Brazil, China, Ecuador, India, Malaysia, tw Taiwan and Thailand (food and agricultural organization 2002).It was reported in 2000, also separately have more than 30 countries to produce this kind.According to New (2000), Vietnam Ye Shiyige major country of production.In addition, Macrobrachium rosenbergii also occupies very important position in fishing for fishery, other several countries in Ru Bangladesh, India and Southeast Asia.
China, since 1976 introduce Macrobrachium rosenbergii cultivation, has basically formed an entire industries system of growing seedlings, become shrimp aquaculture and the processing of meat shrimp at present.Within 2009, national shrimp seedling demand reaches 30,000,000,000 tails, and the cultivation gross area has expanded to more than 70 ten thousand mu, more than 20 ten thousand tons of gross yields.
Macrobrachium rosenbergii leaves original habitat, introduces a fine variety weather, and hand-feeding propagation is for many years gone through in the waters that hydro_geography environmental condition is all not quite similar, and will touch unavoidably the biological or chemical pollution that various mankind's activities cause; Also, because lack antigenic action before self, lack enough antibody, be difficult to resist infection and the invasion and attack of the autochthons kind of raising ground, form prawn disease.Have it, nutrient component is relatively single again, the artificial feed that somatotropin is generally abused and a multitude of names, and the various antibiotic that composition is different, medical drugs prawn body normal growth is grown also can bring drawback.The direct or indirect germplasm that causes Macrobrachium rosenbergii is degenerated.
On October 13rd, 1999 issue, enforcement in 1 day January in 2000, the SC/T1033.1-1999 Macrobrachium rosenbergii cultural technique standard parent shrimp of People's Republic of China's marine industry standard.This requirement differentiates that for the proterties of Macrobrachium rosenbergii part is only simply long to body and body weight has been done requirement, to Macrobrachium rosenbergii seed selection research, fail to provide corresponding technical support, make at present relevant Macrobrachium rosenbergii germplasm select technical operation part with certain blindness.
Summary of the invention
The object of the invention is to solve above-mentioned the deficiencies in the prior art, provide a kind of and can identify easily the Macrobrachium rosenbergii that has specialized character, improved the proterties discrimination method of Macrobrachium rosenbergii of the success rate of Macrobrachium rosenbergii good variety selection.
The present invention is achieved by following technology:
A proterties discrimination method for Macrobrachium rosenbergii, it comprises the operation of numbering, classification, measurement, data processing and analysis result, its concrete operation step is as follows:
(1) numbering: number on the position of periproct AB, tail belly BC, cephalothorax CD and E Ci DE successively;
(2) classification: classify by body length and body weight;
(3) measure: the numerical value on the periproct AB of the every tail Macrobrachium rosenbergii in measuring every type respectively, tail belly BC, cephalothorax CD and E Ci DE, and by each numerical value respectively divided by the sample size in type, draw the mean value at the type sample periproct AB, tail belly BC, cephalothorax CD and E Ci DE place;
(4) numerical value is processed: the ratio that calculates respectively the mean value at tail belly BC, the cephalothorax CD of step (3) gained and the mean value at E Ci DE place and periproct AB place;
(5) analysis result: contrast all types of samples and group types sample by the attach ratios mean value of all measurement points of step (4) gained, the Macrobrachium rosenbergii of the obvious type of numerical value change is specific type pond crayfish.
In above-described numbering step, for the Macrobrachium rosenbergii type that is greater than 20g/ tail, in the second FG of uromere place numbering, and measure as stated above successively, process and analyze this numerical value.
The position of Macrobrachium rosenbergii and normal growth situation are in Table 1.
Table 1: the numbering mark of each measuring point and explanation mark
| numbering | position | remarks |
| aB | periproct | privileged site.Retreat escape, self-defence effect.Without regenerative system. |
| bC | tail belly | reflection dressing percentage situation, numerical value is directly proportional to dressing percentage size.Without regenerative system. |
| cD | cephalothorax | internal organs place, numerical values recited reflection physiological mechanism.Without regenerative system. |
| dE | e Ci | intimidate attack, self-defence effect.Without regenerative system. |
| fG | the second uromere | reflection dressing percentage situation, numerical value is directly proportional to dressing percentage size.Without regenerative system. |
Beneficial effect of the present invention is as follows:
1, Characters Identification method of the present invention, the measurement result of all types sample, tail belly BC, cephalothorax CD, volume thorn DE and the second uromere FG can have corresponding variation along with the different growth types of Macrobrachium rosenbergii from the ratio of periproct AB, this numerical value change of specific type colony exists compared with notable difference, therefore, when each dissimilar individuality of Macrobrachium rosenbergii is selected and remain, can apply this proterties discrimination method, find that as early as possible specific type is individual, improved the success rate of Macrobrachium rosenbergii good variety selection;
2, method of operating of the present invention is simple, convenient, can determine little individual type according to the ratio of all types of tail belly BC, cephalothorax CD, volume thorn DE and the second uromere FG and periproct AB, filters out effectively and accurately the Macrobrachium rosenbergii that has specialized character.
Accompanying drawing explanation
Fig. 1 is the mark schematic diagram at each position, measurement point place of the present invention;
Fig. 2 is the operating process route map of the proterties discrimination method of a kind of Macrobrachium rosenbergii of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described.
Embodiment 1:
At Guangxi aquatic products research institute Macrobrachium rosenbergii seed multiplication farm, catch 500 tail Macrobrachium rosenbergiis as sample, sample content covers the individuality of each different growth types of Macrobrachium rosenbergii.As shown in Figure 1, on shrimp, locate each measurement point, and numbering, on the position of periproct AB, tail belly BC, cephalothorax CD and E Ci DE, numbering successively successively.Be greater than the Macrobrachium rosenbergii type of 20g/ tail, in the second uromere FG place numbering.
By different body length or body weight, sample is divided into 13 types, classification of type is as following table 2.
Table 2: classification of type table
Measure the AB of sample under each type, BC, CD, DE, FG numerical value, and by each numerical value respectively divided by sample size (tail), obtain the AB of the type sample, BC, CD, DE, FG mean value.Then obtain respectively BC, CD, DE, the ratio of FG and AB, result of calculation is as following table 3.
Table 3: the AB of all types sample, BC, CD, DE, the attach ratios table of FG
| Individual type | AB | BC | CD | DE | FG |
| S | 1 | 3.125 | 1.5 | 1.375 | |
| Ⅰ | 1 | 2.7 | 1.27 | 1.9 | |
| Ⅱ | 1 | 3.8 | 2.0 | 2.6 | |
| Ⅲ | 1 | 3.3 | 2.1 | 2.4 | |
| Ⅳ | 1 | 3.58 | 2.35 | 2.32 | 1.5 |
| Ⅴ | 1 | 3.6 | 2.5 | 2.0 | 1.38 |
| Ⅵ | 1 | 3.73 | 2.33 | 2.21 | 1.6 |
| Ⅶ | 1 | 3.37 | 2.2 | 2.04 | 0.86 |
| Ⅷ | 1 | 3.66 | 2.52 | 2.31 | 1.59 |
| Ⅸ | 1 | 3.94 | 2.7 | 2.25 | 1.58 |
| Ⅹ | 1 | 3.83 | 2.83 | 2.25 | 1.58 |
| Ⅺ | 1 | 3.62 | 2.6 | 2.06 | 1.01 |
| Ⅻ | 1 | 3.08 | 2.06 | 1.77 | 1.06 |
| Add up to | 13 | 45.335 | 28.96 | 27.485 | 12.04 |
| On average | 1 | 3.48 | 2.23 | 2.11 | 1.34 |
By table 3, drawn, the AB of all types of samples, BC, CD, DE, the attach ratios mean value of FG:
AB:BC:CD:DE:FG＝1:3.48：2.23：2.11:1.34
When sampling, find that there is the childlike little individual Macrobrachium rosenbergii of part.According to the long different range of body, be divided into 4 types, as following table 4.
Table 4: the classification chart of little individual Macrobrachium rosenbergii
| Type | M-1 | M-2 | M-3 | M-4 |
| The long scope (cm) of body | 3-5 | 6-7 | 7-8 | 9-11 |
Measure the AB of sample under each type, BC, CD, DE, numerical value, and by each numerical value respectively divided by sample size (tail), obtain the AB of type sample, BC, CD, DE mean value.Then obtain respectively BC, CD, the ratio of DE and AB, result of calculation is as following table 5.
Table 5: the result of calculation table of little individual Macrobrachium rosenbergii type
| Individual type | AB | BC | CD | DE |
| M-1 | 1 | 3.72 | 1.9 | 2.62 |
| M-2 | 1 | 3.06 | 1.82 | 2.13 |
| M-3 | 1 | 3.92 | 2.17 | 2.45 |
| M-4 | 1 | 3.07 | 2.10 | 2.0 |
| Add up to | 4 | 13.77 | 7.99 | 9.2 |
| On average | 1 | 3.44 | 1.99 | 2.3 |
The AB of 4 type samples, BC, CD, the attach ratios mean value of DE is: the AB of the AB:BC:CD:DE=1:3.44:1.99:2.3 little individual type of contrast and all types sample, BC, CD, the attach ratios mean value of DE, as following table 6.
Table 6: the mean value difference value of little individual type and all types sample
| Individual type | AB | BC | CD | DE |
| All types sample | 1 | 3.48 | 2.23 | 2.11 |
| Little individual type sample | 1 | 3.44 | 1.99 | 2.3 |
| Difference | - | 0.04 | 0.24 | 0.19 |
By table 6, can be drawn, little individual type sample B C, CD, there is obvious otherness in the ratio of the ratio of DE and AB and all types sample, wherein the otherness of the attach ratios mean value of cephalothorax CD and periproct AB is obvious, and the otherness of the attach ratios mean value of volume thorn DE and periproct AB is more obvious.Therefore, when each dissimilar individuality of Macrobrachium rosenbergii is selected and remain, can apply this proterties discrimination method, find that as early as possible little individual type is individual, thus the Macrobrachium rosenbergii of the merit of can selecting and remain out.
Claims (2)
1. a proterties discrimination method for Macrobrachium rosenbergii, is characterized in that: it comprises the operation of numbering, classification, measurement, data processing and analysis result, and its concrete operation step is as follows:
(1) numbering: number on the position of periproct AB, tail belly BC, cephalothorax CD and E Ci DE successively;
(2) classification: classify by body length and body weight;
(3) measure: the numerical value on the periproct AB of the every tail Macrobrachium rosenbergii in measuring every type respectively, tail belly BC, cephalothorax CD and E Ci DE, and by each numerical value respectively divided by the sample size in type, draw the mean value at the type sample periproct AB, tail belly BC, cephalothorax CD and E Ci DE place;
(4) numerical value is processed: the ratio that calculates respectively the mean value at tail belly BC, the cephalothorax CD of step (3) gained and the mean value at E Ci DE place and periproct AB place;
(5) analysis result: contrast all types of samples and group types sample by the attach ratios mean value of all measurement points of step (4) gained, the Macrobrachium rosenbergii of the obvious type of numerical value change is specific type pond crayfish.
2. the proterties discrimination method of a kind of Macrobrachium rosenbergii according to claim 1, it is characterized in that: in described numbering step, for the Macrobrachium rosenbergii type that is greater than 20g/ tail, in the second FG of uromere place numbering, and measure as stated above successively, process and analyze this numerical value.
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| CN109022589A (en) * | 2018-08-10 | 2018-12-18 | 佛山科学技术学院 | One kind SNP marker relevant to Macrobrachium rosenbergii body length and periproct width and its application |
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