CN103141418B - In-vitro long-acting labeling method applicable to stichopus japonicus - Google Patents
In-vitro long-acting labeling method applicable to stichopus japonicus Download PDFInfo
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- CN103141418B CN103141418B CN201310073006.7A CN201310073006A CN103141418B CN 103141418 B CN103141418 B CN 103141418B CN 201310073006 A CN201310073006 A CN 201310073006A CN 103141418 B CN103141418 B CN 103141418B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to the technical field of aquaculture, in particular to an in-vitro long-acting labeling method applicable to stichopus japonicas. The method comprises the following steps: penetrating a labeling line from the mouth of the stichopus japonicas; after penetrating a lime ring, penetrating out of the body from the position above the head of the stichopus japonicas; fixing a tag capable of identifying the stichopus japonicas on the penetrated labeling line; and stably closing the labeling line to realize the in-vitro long-acting labeling of the stichopus japonicas. By adoption of the labeling method, the stichopus japonicas individual can maintain the in-vitro tag for more than 3 months, the normal activity of the stichopus japonicas individual is influenced hardly, and various field labeling release tests and other work which needs to accurately identify the stichopus japonicas individual after the stichopus japonicas individual is labeled can be further developed.
Description
Technical field
The present invention relates to technical field of aquaculture, specifically a kind of external long-acting labeling method that is applicable to stichopus japonicus.
Background technology
Stichopus japonicus is one of important mariculture kind of China.In aquaculture production, prevalent variety cultivation and the scientific experiment process of stichopus japonicus, usually to carry out mark and Tracking Recognition to it.In numerous biomarker methods, external tracer method is the method for the identification marking biology of the most directly perceived, most convenient, but due to stichopus japonicus body wall softness, whole body is several without hard structure, and stichopus japonicus has very strong rejection ability, be therefore difficult to the external labeling method of other marine organisms (as fish) to be applied on stichopus japonicus.Successful external labeling method must meet two conditions: mark can be retained for a long time; Be labeled biology and can keep normal behaviour activity.Pre-stage test shows, just can self falling after adopting external label 1-2 days that conventional body wall puncture method or mounting method hang in its body surface system, and very large to stichopus japonicus damage.Other have molecular labeling method and osteocomma fluorescent staining tracer method, but its shortcoming is on-the-spot None-identified for the labeling method of stichopus japonicus, must gather after individuality by laboratory experiment or use special inspecting equipment to detect.Therefore, need a kind of stichopus japonicus, reliable and stable external labeling method of being applicable to of exploitation badly, this provides important technical support by the census for carrying out stichopus japonicus, stock enhancement effect tracking and monitoring and praxiology research.Calcareous ring is the peculiar calcareous organ of stichopus japonicus, is positioned at that it is pharyngeal, and the organs such as, nerve ring pharyngeal for supporting and ring water pipe play an important role, and its outstanding feature is closed hard loop configuration.If utilize this structure, develop the external labeling method of a kind of long-acting stichopus japonicus just very hopeful.
Summary of the invention
The object of the invention is to provide a kind of external long-acting labeling method that is applicable to stichopus japonicus.
For achieving the above object, the technical solution used in the present invention is:
The present invention utilizes calcareous ring as external mark system's extension position, has captured the external mark of stichopus japonicus and has been difficult to a long-term difficult problem of retaining; The present invention has stipulated marking operation equipment and necessary technical parameter simultaneously, has guaranteed that the stichopus japonicus after mark can survive and normally ingest.
Be specially:
A kind of external long-acting labeling method that is applicable to stichopus japonicus, mark line is passed external in stichopus japonicus above-head position from the oral area of stichopus japonicus penetrates and pass its calcareous ring, the then fixing label that can identify stichopus japonicus on the mark line passing, finally make mark line secure closure, realize the external long-acting mark of stichopus japonicus.
The described stichopus japonicus for mark should be selected disease-free, undamaged healthy stichopus japonicus.
Describedly mark line is to 45° angle from the oral area of stichopus japonicus and its health direction penetrates, through after its calcareous ring, in its above-head position, pass, tie a knot after fixing suitable label on mark line.
After described mark line closure, mark line to the maximum normal distance of stichopus japonicus body wall is less than 0.5cm.Described fixing label is the label that weight is less than the total weight in wet base 5% of stichopus japonicus.The pin that described use is marked is sewing-needle, embroidery needle or other hard pin that diameter is less than 0.4mm.Described mark line can be nylon setline that wire diameter is less than 0.2mm, operation suture thread or other has some strength, ganoid line.
The invention has the beneficial effects as follows:
1, adopt the present invention, stichopus japonicus label can be retained for a long time, has avoided being discharged by stichopus japonicus, and stichopus japonicus can retain its external label more than at least 3 months, solved the difficult problem that stichopus japonicus is difficult to carry out long-term external mark, and the stichopus japonicus of mark can keep normal feeding activity.
2. adopt the present invention, can be stichopus japonicus system and hang different types of external identification label, as electronic tag, color label, acoustics label etc., after stichopus japonicus mark, can further carry out release experiment and other of various fields mark and need accurately work of identification stichopus japonicus, as mark is recaptured, parentage identification and behaviouristics tracking etc., broken and carried out the technical bottleneck that above work faces.
3. the present invention has wider applicability, applicable to all kinds with closed calcareous ring structure in Holothuroidea.
Accompanying drawing explanation
The external labeling method schematic diagram that is applicable to stichopus japonicus that Fig. 1 provides for the embodiment of the present invention.In figure, 1 label; 2 mark lines; 3 stichopus japonicus oral areas
Embodiment
Be applicable to an external long-acting labeling method for stichopus japonicus, for the stichopus japonicus of mark, should select disease-free, undamaged healthy stichopus japonicus.The fine needle that is less than 0.4mm with diameter is 45° angle from the oral area 3 of stichopus japonicus with its health direction by mark line 2 and penetrates, and through after its calcareous ring, in its above-head position, passes, and fixes the rear knotting of suitable label 1 on mark line.After mark line closure, distance body wall maximum distance is less than 0.5cm.Mark line 2 can be thin ganoid nylon setline, operation suture thread or other and has the line of some strength.Label 1 weight fixing on mark line 2 is less than 5% of the total weight in wet base of stichopus japonicus, can be used for for common color label, electronic tag or other label of individual identification.
Embodiment 1: adopt nylon setline, color plastic label stichopus japonicus
Select the nylon setline of the 0.16mm diameter line that serves as a mark, with the stichopus japonicus of plastic label (weight is 1g) the mark body weight 100g left and right of length 8mm different colours.By label through on mark line, the embroidery needle that is 0.35mm with diameter is 45° angle from the oral area of stichopus japonicus and its health direction and penetrates, through after its calcareous ring, in its above-head position, pass, mark line was drawn to calcareous ring, afterwards adjust knotting position, make linear distance stichopus japonicus body wall be no more than 0.5cm, with electric iron by line sintering, cut off the end of a thread, label installation.The stichopus japonicus label hung by the party's genealogy of law fell label rate lower than 10% in 3 days, and the label of reservation can firmly be retained more than 3 months, and the stichopus japonicus of mark just can recover feeding activity after 12 hours, reach normal food ration after 3 days.The label of different colours is convenient to identification under water in sea area, diver's searching and the stichopus japonicus of recapturing are very convenient.
Embodiment 2: adopt monofilament polypropylene operation suture thread, PIT electronic tag mark stichopus japonicus
Select the monofilament polypropylene operation suture thread line that serves as a mark, the PIT electronic tag of long 6mm, diameter 1mm (weight is 2g) and mark line are fixed together with one little section of plastic bushing, the stichopus japonicus of mark 100g left and right.The embroidery needle that is 0.35mm with diameter is 45° angle by nonabsorable operation suture thread from the oral area of stichopus japonicus and its health direction and penetrates, through after its calcareous ring, in its above-head position, pass, mark line was drawn to calcareous ring, adjust afterwards knotting position, make linear distance stichopus japonicus body wall be no more than 0.5cm, manual knotting, cuts off the end of a thread, label installation.The stichopus japonicus label hung by the party's genealogy of law fell label rate lower than 13% in 4 days, and the label of reservation can firmly be retained more than 3 months, and the stichopus japonicus of mark just can recover feeding activity after 12 hours, reach normal food ration after 3 days.This label is equipped with special code reader, and each label has unique electronic code, is convenient to identification.Stichopus japonicus throw in before sweep record coding, scanning again after recapture, can obtain the significant data such as population density, individual growth of stichopus japonicus.
Embodiment result shows, adopts mark stichopus japonicus of the present invention convenient, reliable, is convenient to identification, is the effective ways that are applicable to stichopus japonicus or the external mark of other sea cucumber.
Claims (7)
1. one kind is applicable to the external long-acting labeling method of stichopus japonicus, it is characterized in that: mark line is passed external in stichopus japonicus above-head position from the oral area of stichopus japonicus penetrates and pass its calcareous ring, the then fixing label that can identify stichopus japonicus on the mark line passing, finally make mark line secure closure, realize the external long-acting mark of stichopus japonicus.
2. by the external long-acting labeling method that is applicable to stichopus japonicus claimed in claim 1, it is characterized in that: the described stichopus japonicus for mark should be selected disease-free, undamaged healthy stichopus japonicus.
3. by the external long-acting labeling method that is applicable to stichopus japonicus claimed in claim 1, it is characterized in that: describedly mark line is to 45° angle from the oral area of stichopus japonicus and its health direction penetrates, through passing in its above-head position after its calcareous ring, tie a knot after fixing suitable label on mark line.
4. by the external long-acting labeling method that is applicable to stichopus japonicus described in claim 1 or 3, it is characterized in that: after described mark line closure, mark line to the maximum normal distance of stichopus japonicus body wall is less than 0.5cm.
5. by the external long-acting labeling method that is applicable to stichopus japonicus described in claim 1 or 3, it is characterized in that: described fixing label is the label that weight is less than the total weight in wet base 5% of stichopus japonicus.
6. by the external long-acting labeling method that is applicable to stichopus japonicus described in claim 1 or 3, it is characterized in that: using the pin of marking is sewing-needle or the embroidery needle that diameter is less than 0.4mm.
7. by the external long-acting labeling method that is applicable to stichopus japonicus described in claim 1 or 3, it is characterized in that: described mark line is nylon setline or the operation suture thread that wire diameter is less than 0.2mm.
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| CN103749359B (en) * | 2014-01-09 | 2016-01-06 | 大连海宝渔业有限公司 | Sea urchin individual marking method |
| CN106561525B (en) * | 2016-11-07 | 2019-06-25 | 浙江省海洋水产研究所 | A kind of fish tag label, fish tag device and denotation approach |
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| US4790090A (en) * | 1987-04-30 | 1988-12-13 | Sharber Norman G | Fish tag |
| US4970988A (en) * | 1990-02-01 | 1990-11-20 | Rmc Environmental Services, Inc. | Method and apparatus for monitoring animal migration |
| CN1561845A (en) * | 2004-04-15 | 2005-01-12 | 尹宝昌 | Method for carving mark on sea cucumber |
| JP5024985B2 (en) * | 2006-09-14 | 2012-09-12 | 独立行政法人水産総合研究センター | Fish labeling and labeling methods |
| CN201528585U (en) * | 2009-10-14 | 2010-07-21 | 上海海洋大学 | In vitro shroud ring marking device and marking aid thereof |
| CN201557455U (en) * | 2009-11-23 | 2010-08-25 | 大连水产学院 | Sea urchin individual marking device |
| CN101785438B (en) * | 2010-01-29 | 2015-02-11 | 中国科学院海洋研究所 | Method for marking stichopus japonicus individual and detecting mark |
| CN102165924A (en) * | 2011-01-20 | 2011-08-31 | 中国科学院南海海洋研究所 | Fluorescence labeling method for sea cucumber larvas |
| CN202013698U (en) * | 2011-04-18 | 2011-10-19 | 李佳沁 | Sea cucumber with identification |
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