CN103131784A - Multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum - Google Patents
Multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum Download PDFInfo
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Abstract
The invention relates to a multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum. The multi-PCR method comprises the following steps of: with the extracted DNA (deoxyribonucleic acid) of a bacterium to be detected as a template, carrying out mPCR (multiple polymerase chain reaction) amplification on five pairs of primers as shown in SEQ ID NO.1-10; and carrying out agarose gel electrophoresis on mPCR amplification products, and then determining according to electrophoresis results. According to the multiple PCR method, the synthesized primers are used for carrying out PCR amplification, a diseased material can be directly ground and amplified by adopting the established multiple PCR, different salmonella serotypes can be detected in one system, serum glass plate agglutination is completed in only about six hours compared with three days for the traditional serum glass plate agglutination after separation pure culture in clinical detection, the diagnosis time is greatly shortened, and a basis is provided for clinical diagnosis.
Description
Technical field
The present invention relates to a kind of method that multiplex PCR detects the Salmonellas different serotypes, the serotype that relates generally to is Salmonella enteritidis, Salmonella typhimurium, white dysentery Salmonellas and fowl typhoid Salmonellas.
Background technology
Salmonellas (Salmonella) is the general name of a class common bacteria, and the people that only causes who wherein has falls ill, and the animal that only causes that has falls ill, and also has some that humans and animals is all caused a disease.Salmonellosis (Samonellosis) refers to by the caused disease general name multi-form to the mankind, domestic animal and wildlife of various types of Salmonellass.
After pig, fowl, dog, cat infect Salmonellas, can cause the symptoms such as gastro-enteritis, miscarriage, septicemia.Along with the expansion of animal intensive farm scale, and antibacterials being widely used in clinical and animal-feed, the Salmonellas resistance is on the rise, and the infection rate in many animals rises gradually, and the financial loss that causes is huge.Therefore, very important with the reduction financial loss to timely diagnosis and the purification prevention of disease of animal salmonellosis.Salmonellas can pollute the food such as meat, egg, milk, makes the human hair uncooked food poisoning.According to statistics, in the various bacterial food poisoning diseases of global human, the normal row umber one of salmonellal food poisoning.Therefore, this disease is of great significance in people doctor, animal doctor and public health, therefore, set up a kind of technology that detects fast and accurately Salmonellas, can in time determine pathogenic agent, be conducive to people and in time take effective prevention and control measure, plant's financial loss is significant for reducing.
In recent years, PCR method is used in the detection of Salmonellas widely, and the method can make up the deficiency of the classical detection method of Salmonellas, and has good specificity and susceptibility.1992, at first Rahn etc. designed pair of primers, detect Salmonellas with PCR, and recall rate is 97%, and afterwards, round pcr is widely used in harmful microbe and detects.At present, the primer that detects for salmonella PCR mainly is divided three classes: belong to specific gene primer, serogroups specific gene primer and serotype specific gene primer.The genus specific gene is the conserved sequence in salmonella, and having or not of this sequence is to judge whether bacterial strain belongs to the foundation of Salmonellas.the PCR method for quick of Salmonellas is normally based on the gene order that belongs to specific gene, it belongs to specific gene and mainly contains hut gene (encoding histidine transhipment operon), inv gene cluster (coding absorption and invasion and attack surface epithelial cell albumen), hil gene (coding invasin gene positive regulator), fim gene (the main subunit of coding I type pili), hns gene (DNA of coding and protein bound), spv gene (the relevant virulence factor of coding Salmonellas toxicity plasmid) and 16SrRNA gene (rrna important component part).Rahn K etc., Stone G G etc., Lu Qiang etc. all confirm to have the genus specificity based on the primer of inv gene cluster design, can be used for differentiating Salmonellas.The rRNA of bacterium is the maximum RNA of cell intensive amount, accounts for more than 80% of RNA total amount, and its molecular weight is large, and kind is many, is comprised of high conservative region and variable region.The rRNA of bacterium comprises 5S, 16S, 23S, and wherein 5S rRNA quantity of information is few, is not suitable for analyzing.Although 23S rRNA molecule is large, quantity of information is many, and the base mutation speed is not suitable for Bacteria Identification equally.The heredity of 16S rRNA is comparatively stable by contrast, and length is in 1550 ± 200bp left and right, and the representative information amount is moderate, is the good material that Study system is evolved.Reported at present the bacterial 16 S rDNA sequence more than 10,000 kinds.By the sequential analysis to 16S rDNA, also can be used for the preliminary evaluation of Salmonellas.
Development along with molecular engineering, developed multiplex PCR (multiplex PCR, mPCR) technology on the basis of PCR, multiplex PCR is better used in the check of food-borne pathogens, a plurality of genes of a kind of pathogenic bacterium can be detected simultaneously, also various pathogens can be detected simultaneously.Set up as Soo Jin Yang etc. the multiplex PCR of identifying animal derived multidrug resistant Salmonella typhimurium and Salmonella enteritidis; Chai Fung Pui etc. has set up the multiple PCR method that detects salmonella typhi and Salmonella typhimurium; Min-Su Kang etc. has set up the multiple PCR method that in the chicken Salmonella enteritidis, white dysentery and fowl typhoid and fowl typhoid living vaccine strain 9R are identified.This technology has made up the deficiency of the classical detection method of Salmonellas, and has good specificity and susceptibility.We are according to salmonella specific gene hut design pair of primers, in addition, according to Salmonella enteritidis (Sdf I), Salmonella typhimurium (SPY), white dysentery Salmonellas (glgc), the serotype specificity gene of fowl typhoid Salmonellas (glgc and Spec) designs respectively pair of primers.Whether the multiplex PCR of setting up can detect simultaneously and identify cause of disease is Salmonella enteritidis, Salmonella typhimurium, white dysentery Salmonellas and fowl typhoid Salmonellas.
Summary of the invention
This research is further explored the PCR method that detects Salmonellas, on the genus specificity and the basis of serotype specificity of other people research, design genus and type specificity primer for the specific gene of four kinds of common serotypes of bird, set up the detection method of multiplex PCR.The method not only can Rapid identification go out Salmonellas in same PCR reaction system, and can determine whether it is Salmonella enteritidis, Salmonella typhimurium, white dysentery Salmonellas or fowl typhoid Salmonellas.
The present invention is according to salmonella specific gene hut design pair of primers, in addition, according to Salmonella enteritidis (Sdf I), Salmonella typhimurium (SPY), white dysentery Salmonellas (glgc), the serotype specificity gene of fowl typhoid Salmonellas (glgc and Spec) designs respectively pair of primers.Each serotype of primer pair with design is carried out pcr amplification, sets up multi-PCR detection method.
The technical solution adopted in the present invention is: take the tested bacteria DNA that extracts as template, carry out mPCR with following 5 pairs of primers and increase; Then pcr amplification product is judged according to electrophoresis result through agarose gel electrophoresis; What wherein amplify 500bp and 304bp band is Salmonella enteritidis, what amplify 500bp and 401bp band is Salmonella typhimurium, what amplify 500bp and 252bp band is the white dysentery Salmonellas, amplifies the fowl typhoid Salmonellas of 500bp, 252bp and 174bp band.
The primer that utilization of the present invention is synthesized carries out pcr amplification, the multiplex PCR of setting up can directly grind pathological material of disease and increase, can detect different Salmonellas serotype in an individual system, the 3 day time of the more traditional clear glass plate of the laggard promoting circulation of blood of separation pure culture aggegation needs about 6 hours than only in clinical detection, greatly shortened Diagnostic Time, for clinical diagnosis provides foundation.
Description of drawings
Fig. 1: multiplex PCR specificity experimental result
Wherein 1: Salmonella choleraesuls, 2: Newport Salmonellas, 3: Pparatyphoid A, 4: Salmonella abortus bovis, 5: Salmonella typhimurium, 6: fowl typhoid Salmonellas, 7: white dysentery Salmonellas, 8: Salmonella enteritidis, 9: riemerella anatipestifer, 10: intestinal bacteria, M:100bp ladder marker, 11: negative control.
Fig. 2: multiplex PCR sensitivity test result
Wherein the M left side is the susceptibility of Salmonella enteritidis; M the right is the susceptibility of Salmonella typhimurium.
Fig. 3: multiplex PCR sensitivity test result
Wherein the M left side is the susceptibility of fowl typhoid Salmonellas; M the right is the susceptibility of white dysentery Salmonellas.
Fig. 4: multiplex PCR broad spectrum test-results
Wherein 1: Salmonella typhimurium 1,2: fowl typhoid Salmonellas, 3: white dysentery Salmonellas 1,4: Salmonella enteritidis 1,5:100bp ladder marker, 6: Salmonella enteritidis 2,7: white dysentery Salmonellas 2,8: fowl typhoid Salmonellas, 9: Salmonella typhimurium 2,10: Salmonella typhimurium 3,11: the fowl typhoid Salmonellas, 12: white dysentery Salmonellas 3,13: Salmonella enteritidis 3,14:100bp ladder marker.
Embodiment
Concrete technical scheme of the present invention is as follows:
1. the design of primer
According to experiment purpose, through homology relatively, according to salmonella specific gene hut design pair of primers, in addition, according to Salmonella enteritidis (Sdf I), Salmonella typhimurium (SPY), white dysentery Salmonellas (glgc), the serotype specificity gene of fowl typhoid Salmonellas (glgc and Spec) designs respectively pair of primers.Primer sequence is as follows:
Annotate: annex base code R=A/G Y=C/T.
2. the extraction of DNA of bacteria
(1) the single bacterium colony of picking shakes bacterium and spends the night in 3ml liquid LB substratum, get 400 μ L bacterium liquid with the 1.5ml centrifuge tube, the centrifugal 5min of 12000rpm abandons supernatant, with the 200 centrifugal 5min of the resuspended rear 12000rpm of the aseptic ultrapure water of μ L, abandon supernatant, resuspended with the 100 aseptic ultrapure waters of μ L, boil 10min, ice bath 10min, centrifugal 5 minutes of 12000rpm gets supernatant.
(2) extract with bacterial genomes DNA small volume of reagent box.
3.mPCR amplifying target genes fragment
Adopt 25 μ L systems: 10 * Buffer2.5 μ L, Mg
2+1.5 μ L, dNTP0.2 μ L, each 0.5 μ L(concentration of primer hut-F and hut-R, SE-F and SE-R, SPY-F and SPY-R, SG-F and SG-R, SGP-F and SGP-R is 25 μ mol/ μ L), TaqDNA polysaccharase (1U/ μ L) 1.5 μ L, DNA profiling 2 μ L, with aseptic ultrapure water polishing to 25 μ L.Response procedures: 94 ° of C denaturation 4min, 94 ° of C sex change 45s, 56 ° of C annealing 30s, 72 ° of C extend 45s, 30 circulations, 72 ° of C extend 10min.
4.mPCR the evaluation of amplified production
Multiple PCR products is added get 13 μ L after the loading damping fluid and be added in 2% sepharose, take 100bpladder marker as standard reference, after running 1.5h with 80 volts of voltages, observations after dyeing, wherein Salmonella enteritidis amplifies the band of 500bp and 304bp, Salmonella typhimurium amplifies the band of 500bp and 401bp, and the white dysentery Salmonellas amplifies the band of 500bp and 252bp, and the fowl typhoid Salmonellas amplifies the band of 500bp, 252bp and 174bp.The Salmonellas of other serotypes only has the band of 500bp, and non-Salmonellas riemerella anatipestifer and intestinal bacteria and stealthy contrast all occur without band.In addition, positive band is cut sent order-checking after glue reclaims, amplified band is consistent with the goal gene sequence.Result such as Fig. 1:
5. multiplex PCR sensitivity test
Extract Salmonella enteritidis with bacterial genomes DNA small volume of reagent box, Salmonella typhimurium, the white dysentery Salmonellas, the genome of fowl typhoid Salmonellas type strain, survey after concentration respectively by carrying out multiplex PCR after ten times of doubling dilutions, enteritis DNA stoste 81ng/ μ L, mouse typhus DNA stoste 101ng/ μ L, fowl typhoid DNA stoste 89ng/ μ L, white dysentery stoste 107ng/ μ L.Multiplex PCR result statistics hut-F and hut-R can detect the DNA of 8pg/ μ L, and SE-R and SE-F, SPY-F and SPY-R, SGP-F and SGP-R, SG-F and SG-R can detect 80pg/ μ L.Result such as Fig. 2 and Fig. 3:
6. multiplex PCR broad spectrum test-results
Choose the white dysentery Salmonellas, Salmonella typhimurium, each three strains of Salmonella enteritidis and fowl typhoid Salmonellas type strain carry out identification with multi-plex PCR, two specific bands appear in white dysentery Salmonellas, Salmonella typhimurium and Salmonella enteritidis, and three specific bands appear in the fowl typhoid Salmonellas.Result such as Fig. 4.
Claims (2)
1. multiple PCR method of differentiating Salmonella enteritidis, Salmonella typhimurium, white dysentery Salmonellas and fowl typhoid Salmonellas, it is characterized in that, take the tested bacteria DNA that extracts as template, carry out mPCR with 5 pairs of primers shown in SEQ ID NO.1-10 and increase; Then the mPCR amplified production is judged according to electrophoresis result through agarose gel electrophoresis.
2. method according to claim 1, is characterized in that; What electrophoresis amplified 500bp and 304bp band is Salmonella enteritidis, what amplify 500bp and 401bp band is Salmonella typhimurium, what amplify 500bp and 252bp band is the white dysentery Salmonellas, amplifies the fowl typhoid Salmonellas of 500bp, 252bp and 174bp band.
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| CN104774969A (en) * | 2015-05-08 | 2015-07-15 | 江苏省家禽科学研究所 | Multi-PCR detection kit and method for identifying poultry salmonella |
| CN104846066A (en) * | 2014-09-23 | 2015-08-19 | 中国农业大学 | PCR detection primers and detection method of Salmonella pullorum |
| CN105087806A (en) * | 2015-09-09 | 2015-11-25 | 江苏省家禽科学研究所 | Primer group, kit and method for identifying salmonella pullorum and salmonella gallinarum |
| CN105648055A (en) * | 2016-01-15 | 2016-06-08 | 江苏省家禽科学研究所 | Multi-PCR detection kit for poultry salmonella and non-diagnostic detection method of poultry salmonella |
| CN106086209A (en) * | 2016-08-03 | 2016-11-09 | 扬州大学 | A kind of Rapid identification Pullorum Disease and the PCR detection kit of Salmonella gallinarum |
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| WO2017219597A1 (en) * | 2016-06-22 | 2017-12-28 | 扬州大学 | Pcr detection kit for rapidly identifying salmonella of specific serotypes |
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| CN109182568A (en) * | 2018-10-12 | 2019-01-11 | 安徽九天英诺动物药业有限公司 | A kind of salmonella Rapid identification and classifying method and kit |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603422A (en) * | 2004-08-02 | 2005-04-06 | 扬州大学 | Multiplex PCR rapid identification method for salmonella and listeria monocytogenes |
| WO2007085675A1 (en) * | 2006-01-27 | 2007-08-02 | Universidad Del Pais Vasco | Multiple pcr detection and typing of salmonella in food samples |
| CN101235410A (en) * | 2008-01-25 | 2008-08-06 | 广东省微生物研究所 | Multiple PCR rapid detection kit and detection method for pathogen in aquatic products |
| KR20110044427A (en) * | 2009-10-23 | 2011-04-29 | 대한민국(관리부서 : 농림수산식품부 국립수의과학검역원) | Differential identification of salmonella enterica serovar gallinarum biovar gallinarum, pullorum and biovar gallinarum live vaccine strains |
-
2013
- 2013-03-08 CN CN2013100751701A patent/CN103131784A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603422A (en) * | 2004-08-02 | 2005-04-06 | 扬州大学 | Multiplex PCR rapid identification method for salmonella and listeria monocytogenes |
| WO2007085675A1 (en) * | 2006-01-27 | 2007-08-02 | Universidad Del Pais Vasco | Multiple pcr detection and typing of salmonella in food samples |
| CN101235410A (en) * | 2008-01-25 | 2008-08-06 | 广东省微生物研究所 | Multiple PCR rapid detection kit and detection method for pathogen in aquatic products |
| KR20110044427A (en) * | 2009-10-23 | 2011-04-29 | 대한민국(관리부서 : 농림수산식품부 국립수의과학검역원) | Differential identification of salmonella enterica serovar gallinarum biovar gallinarum, pullorum and biovar gallinarum live vaccine strains |
Non-Patent Citations (1)
| Title |
|---|
| EDEL O"REGAN 等: "Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples", 《BMC MICROBIOLOGY》 * |
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| CN105087806A (en) * | 2015-09-09 | 2015-11-25 | 江苏省家禽科学研究所 | Primer group, kit and method for identifying salmonella pullorum and salmonella gallinarum |
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