CN103131733A - Novel method to produce biomass energy by means of biowaste combinations - Google Patents
Novel method to produce biomass energy by means of biowaste combinations Download PDFInfo
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- CN103131733A CN103131733A CN201110399811XA CN201110399811A CN103131733A CN 103131733 A CN103131733 A CN 103131733A CN 201110399811X A CN201110399811X A CN 201110399811XA CN 201110399811 A CN201110399811 A CN 201110399811A CN 103131733 A CN103131733 A CN 103131733A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a novel method to produce biomass energy by means of biowaste combinations. The method is characterized in that biowaste serves as fermentation substrates and comprises food waste, agricultural product waste and mycelium waste produced in the production process of antibiotics. Combinations of the fermentation substrates include two to four kinds of the preceding waste in different proportions. Production engineering bacteria strains are bacillus fusiformis. Produced biomass energy products include acetone, ethanol, butanol and isopropanol. When the biomass energy is produced in the novel method, the biowaste which originally pollutes the environment is fully made use of, the environment is purified, recycling of resources is achieved in the greatest degree, meanwhile, the fermentation efficiency can be obviously improved, cell growth speeds are enhanced, fermentation time is obviously shortened, and fermentation cost is greatly reduced.
Description
Affiliated technical field
Content of the present invention relates to a kind of novel method of utilizing bio-waste combinations produce biomass energy, and a kind of bacillus fusiformis that uses carries out the novel method of fermentation to produce biological mass-energy to the formula combination of different bio-wastes as substrate, belongs to the bioenergy technology.
Background technology
Since the 1970's, many countries and international organization more and more pay close attention to and utilize renewable raw materials to produce thing fuel next life, to alleviate the dependence to oil.Wherein utilize the technology of preparing ethyl alcohol with corn fermentation, butanols to be used, the application of this technology has been opened up and has been utilized the biotechnology production energy to become a reality, and can suitably reduce the unique dependency to oil.
Yet it is such having now and utilizing the technology of corn fermentation production biomass energy ethanol, butanols: corn is first soaked, and grinds correct grinding, screening and centrifugal; Germ oil floating matter after correct grinding is collected, and fiber, centrifugal removal colloid are removed in screening; Then through batch fermentation, starch is changed into ethanol, butanols.The price straight line of corn rises at present, causes this explained hereafter cost kind maize raw material to account for more than 70%.This road with grain transducing source certainly can not be lasting, therefore, seeks a large amount of production substrate at a low price and be used for bioenergy as the substitute of corn and transform to produce and become a most important problem.
In biomass energy, butanols is a kind of suitability bioenergy very widely, and its numerous characteristics is better than gasoline.Its ratio of mixture is 11.2, and gasoline is 14.7, and ethanol is 9, so butanols can directly be used in the engine of standard and need not to change the design of engine.Its fuel value is equivalent to 92% of normal benzene, and in the driving of reality was experienced, the butanols fuel oil can provide sufficient power, efficient Energy efficiency, and suitable in gasoline; In addition, butanols has the very vapor pressure at the end and high flash-point, makes it become a kind of very safe energy, especially makes under hot conditions operation more show its advantage.Therefore butanols directly replacing gasoline or diesel oil use as energy substance, this be ethanol irreplaceable, might become real energy substance of future generation.
The invention provides a kind of novel method of utilizing bio-waste combinations produce biomass energy, a kind of bacillus fusiformis that uses carries out the method for fermentation to produce biological mass-energy butanols, ethanol, acetone and Virahol to the formula combination of different bio-wastes as substrate; Namely utilize food waste thing (FB), comprise vegetables and fruit waste; Agricultural product castoff, as comprise maize straw (CSS), straw and straw (WGS), vinasse (BW), wine junket (DSG), palm pressed liquor (POME); The mycelium waste (CAB) that produces in the production of antibiotics process and other castoffs that can be utilized by bacillus fusiformis are the novel method of substrate production biomass energy.
Summary of the invention
The invention provides a kind of novel method of utilizing bio-waste combinations produce biomass energy, a kind of bacillus fusiformis that uses carries out the method for fermentation to produce biological mass-energy to the formula combination of different bio-wastes as substrate.
Described bio-waste refers to food waste thing (FB), comprises vegetables and fruit waste, as potato, and Caulis et Folium Brassicae capitatae bar, strawberry, orange skin, Pericarpium Musae, Folium Dauci Sativae, Pericarpium Mali pumilae, lettuce leaves and skin, cauliflower leaf; Agricultural product castoff comprises maize straw (CSS), straw and straw (WGS), vinasse (BW), wine junket (DSG), palm pressed liquor (POME); The mycelium waste (CAB) that the production of antibiotics process produces and other castoffs that can be utilized by bacillus fusiformis.
Described bacterial classification is bacillus fusiformis Clostridium acetobutylicum (ATCC 824).
Described formula combination is: these wastes and between them the combination of different ratios can be used as the biological fermentation substrate and use: different wastes can mix use, and these formula combination include but not limited to:
DSG+CAB
POME+CAB
WGS+BW
FB+CSS
CSS+BW
POME+FB
CSS+DSG
The combination of these different bio-wastes can make 2-4 kind wherein, and can design different blending ratios.
Described novel method refer to bio-waste dewater, grind sieve, the technological process of Chemical Pretreatment, enzyme processing, nutrient solution, the combined fermentation that adds bio-waste, collection of biological mass-energy.
Described biomass energy comprises butanols, ethanol, acetone and Virahol.
Novelty of the present invention is: can be with the food waste thing (FB) that originally pollutes the environment, the mycelium waste (CAB) that agricultural product castoff and production of antibiotics process produce fully utilizes, the scientific combination formula uses, as fermentation substrate; Sugar in FB and Mierocrystalline cellulose are directly applied to the growth of thalline and the bio-transformation of the energy as carbon source; With free amino acid in CAB, VITAMIN is as nitrogenous source, for the growth of bacterial classification provides good nutrition, improved the speed of growth, shortened the time of fermentation.Be used in combination the interpolation that these bio-wastes can substitute starch and VITAMIN in the traditional zymotic process, thereby reduce costs significantly, simultaneously environment purification.
Technical scheme
The invention provides a kind of novel method of utilizing bio-waste combinations produce biomass energy, a kind of bacillus fusiformis that uses carries out the novel method of fermentation to produce biological mass-energy to the formula combination of different biological wastes as substrate.
Described bio-waste is that the condition of bacillus fusiformis fermentation substrate is: wine junket (DSG), solid, water content 30-45%; Palm pressed liquor (POME), liquid; Production of antibiotics fermentation wastes (CAB), solid, water content 35-70%; Food waste thing (FB) comprises vegetables and fruit waste, as potato, and Caulis et Folium Brassicae capitatae bar, strawberry, orange skin, Pericarpium Musae, Folium Dauci Sativae, Pericarpium Mali pumilae, lettuce leaves and skin, cauliflower leaf; Maize straw (CSS), solid, water content 8-20%; Straw and straw (WGS), solid, water content 8-20%; Vinasse (BW), solid, water content 30-45%.
Embodiment:
Definition: in this patent text, following term is defined as: DSG: wine junket; POME: palm squeezing waste liquid; CAB: the thalline squeezing solid after production of antibiotics; FB: food waste thing; CSS: maize straw; WGS: straw or straw; BW: vinasse;
The present invention implements like this:
The preparation of material and processing: method provided by the invention makes and adopts the above-mentioned bio-waste of addressing as fermentation substrate, comprises following processing step:
A) anticipating of substrate: for example, the CAB substrate, 80 degree oven dry Celsius are controlled moisture content less than 8%, grind to form 100 purpose powder.
B) chemical treatment: for example, with the CAB powder of 100 grams through 80 degree oven dry, add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour; The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
C) fermentation: under anaerobic inoculate 100 milliliters of work bacterial classifications; 37 degree, anaerobism was cultivated 60-110 hour, gas chromatographic detection butanols, ethanol and acetone composition.
The combination of different bio-waste substrates:
Bio-waste can be used alone in the present invention, also multiple bio-waste can be used in combination.Different bio-wastes can mix according to certain ratio, formula can according to but be not limited to following combination:
DSG+CAB
POME+CAB
WGS+BW
FB+CSS
CSS+BW
POME+FB
CSS+DSG
Advantage of the present invention comprises: A) use bio-waste, therefore reduced material cost; B) using bio-waste as substrate, itself is a kind of method of processing environment waste.For example, the waste of antibiotic fermentation production contains very high biological oxygen demand (BOD), contains again the microbiotic of trace, can not be used for otherwise interpolation and use, and is very unmanageable environment waste, and in the present invention, can directly uses.
The following examples is in order better the present invention to be illustrated and to describe, not representing any restriction to this patent content.Involved technology in this patent is the generally technology of grasp of this field middle rank personnel institute.
The screening of embodiment one substrate:
A) can select following a kind of bio-waste as substrate: wine junket (DSG), solid, water content 30-45%; Palm pressed liquor (POME), liquid; Production of antibiotics fermentation wastes (CAB), solid, water content 35-70%; Food waste thing (FB) comprises vegetables and fruit waste, as potato, and Caulis et Folium Brassicae capitatae bar, strawberry, orange skin, Pericarpium Musae, Folium Dauci Sativae, Pericarpium Mali pumilae, lettuce leaves and skin, cauliflower leaf etc.; Maize straw (CSS), solid, water content 8-20%; Straw and straw (WGS), solid, water content 8-20%; Vinasse (BW), solid, water content 30-45%.
B) different bio-wastes can mix use, and these formulas include but not limited to: DSG+CAB; POME+CAB; WGS+BW; FB+CSS; CSS+BW; POME+FB; CSS+DSG; The combination of these different bio-wastes can make 2-4 kind wherein, and according to different blending ratios.
The pre-treatment of embodiment two DSG: DSG after 80 degree oven dry, controls water content less than 8%, grind into powder.
The pre-treatment of embodiment three CAB: CAB after 80 degree oven dry, controls water content less than 8%, grind into powder.
The pre-treatment of embodiment four FB: FB, homogenate becomes syrupy shape.
The pre-treatment of embodiment five CSS: CSS, natural drying at room temperature is controlled water content less than 8%, grind into powder.
The pre-treatment of embodiment six WGS: WGS, natural drying at room temperature is controlled water content less than 8%, grind into powder.
The pre-treatment of embodiment seven BW: BW after 80 degree oven dry, controls water content less than 8%, grind into powder.
The chemical treatment of embodiment eight DSG: with 100 gram DSG powder (preparing in embodiment two), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The chemical treatment of embodiment nine CAB: with 100 gram CAB powder (preparing in embodiment three), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The chemical treatment of embodiment ten FB: with 100 gram FB powder (preparing in embodiment four), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The chemical treatment of embodiment 11 CSS: with 100 gram CSS powder (preparing in embodiment five), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The chemical treatment of embodiment 12 WGS: with 100 gram WGS powder (preparing in embodiment six), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The chemical treatment of embodiment 13 BW: with 100 gram BW powder (preparing in embodiment seven), add 500 milliliters of 0.16M HNO
3, stirred 12 hours in room temperature, with 10M NaOH neutralization, transfer pH to 4.0-7.8; Better, pH is between 5.0-6.8; Best, pH is between 5.5-6.8; Add cellulase digestion substrate, the 35-50 degree, 150-250RPM stirs, and reacts 8-18 hour.The undissolved solid of centrifugal removal is collected supernatant liquor as nutrient solution.
The preparation of embodiment 14 original strains: with bacterial classification Clostridium acetobutylicum (ATCC 824), preserve liquid from 4 degree and take out, be placed under 80 degree conditions heat-shocked and processed 10 minutes, be inoculated in 10 milliliters of special culture solution, 37 degree anaerobism were cultivated 14 hours.
The preparation of embodiment 15 work bacterial classifications:
Adopt CBM nutrient solution formula: carbon source 10g; Casein hydrolysate 4g; KH
2PO
40.5g; K
2HPO
40.5g; MgSO
47H
2O 0.2g; MnSO
44H
2O 10mg; NaCl 10mg; FeSO
47H
2O 10mg; P-aminobenzoic acid 1mg; Thiamine HCL 1mg; D-biotin 2ug;
Add 1000 ml deionized water, 121 degree high-temperature sterilizations, 20 minutes; Take out 5 milliliters in the primordial seed liquid of describing from embodiment 14, be inoculated in 100 milliliters of above-mentioned CBM nutrient solutions, then add glucose to concentration 6%; 37 degree anaerobism were cultivated 20 hours.
Embodiment 16 fermentations 1
1500 milliliters of 3500 milliliters of nutrient solution: CBM (describing in embodiment 15): DSG (preparation in embodiment eight);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 17 fermentations 2
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of CAB (preparation in embodiment nine);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 18 fermentations 3
500 milliliters of nutrient solution: CBM (describing in embodiment 15); 4500 milliliters of POME (preparation in embodiment one);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 19 fermentations 4
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of WGS (preparation in embodiment 12);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 20 fermentations 5
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of FB (preparation in embodiment ten);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 21 fermentations 6
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of CSS (preparation in embodiment 11);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 22 fermentations 7
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of BW (preparation in embodiment 13);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 23 fermentations 8
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 500 milliliters of CAB (preparation in embodiment nine); 1500 milliliters of DSG (preparation in embodiment eight);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 24 fermentations 9
0 milliliter of nutrient solution: CBM (describing in embodiment 15); 500 milliliters of CAB (preparation in embodiment nine); 4500 milliliters of POME (preparation in embodiment one);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 25 fermentations 10
3000 milliliters of nutrient solution: CBM (describing in embodiment 15);
500 milliliters of CAB (preparation in embodiment nine); 1500 milliliters of WGS (preparation in embodiment 12);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 26 fermentations 11
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 500 milliliters of CAB (preparation in embodiment nine); 1500 milliliters of FB (preparation in embodiment ten);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 27 fermentations 12
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 500 milliliters of CAB (preparation in embodiment nine); 1500 milliliters of CSS (preparation in embodiment 11);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 28 fermentations 13
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 500 milliliters of CAB (preparation in embodiment nine); 1500 milliliters of BW (preparation in embodiment 13);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 29 fermentations 14
3500 milliliters of nutrient solution: CBM (describing in embodiment 15); 750 milliliters of FB (preparation in embodiment nine); 750 milliliters of CSS (preparation in embodiment eight);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 30 fermentations 15
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of CSS (preparation in embodiment 11); 500 milliliters of BW (preparation in embodiment 13);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 31 fermentations 16
2000 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of POME (preparation in embodiment one); 1500 milliliters of FB (preparation in embodiment ten);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
Embodiment 32 fermentations 17
3000 milliliters of nutrient solution: CBM (describing in embodiment 15); 1500 milliliters of CSS (preparation in embodiment 11); 500 milliliters of DSG (preparation in embodiment eight);
Inoculate 100 milliliters of work bacterial classifications (preparation in embodiment 15), adjust PH between 5.5-6.8,37 degree, anaerobism was cultivated 60-110 hour.
In fermenting process, sampling is used the gas chromatographic detection butanols, the content of ethanol and acetone.
The different substrate combined fermentation results that embodiment 33 gas chromatographic detection are analyzed
Result shows, uses separately CAB undesirable to the generation of product, but the time of fermenting has obviously been shortened in the growth that has improved significantly bacillus fusiformis.
POME uses separately, and the quantum of output of biomass energy is very low, uses rear output obviously to improve but unite in FB, and has shortened fermentation time.
The document of quoting in the present invention is just used as a reference.Should be appreciated that, if the minor alteration of the unsubstantiality that the professional person makes after with reference to text of the present invention invention and modify the claims that are similarly this patent and cover.
Claims (4)
1. novel method of utilizing bio-waste combinations produce biomass energy, it is characterized in that bio-waste comprises food waste thing (FB), the mycelium waste (CAB) that agricultural product castoff and production of antibiotics process produce as fermentation substrate; The combination of fermentation substrate comprises in above-mentioned waste the different proportionings of any 2 to 4 kinds; Production engineering bacterium bacterial classification is bacillus fusiformis; The biomass energy product comprises acetone, ethanol, butanols and Virahol.
2. a kind of novel method of utilizing bio-waste combinations produce biomass energy as claimed in claim 1 is characterized in that food waste thing (FB) comprises vegetables, fruit waste, expired food and remaining food; Agricultural product castoff comprises maize straw (CSS), straw and straw (WGS), vinasse (BW), wine junket (DSG), palm pressed liquor (POME); The mycelium waste that the production of antibiotics process produces.
3. a kind of novel method of utilizing bio-waste combinations produce biomass energy as claimed in claim 1, is characterized in that the combination of fermentation substrate includes but not limited to following DSG+CAB; POME+CAB; WGS+BW; FB+CSS; CSS+BW; POME+FB; The various combination of the different proportionings of any 2 to 4 kinds in CSS+DSG or above-mentioned waste.
4. a kind of novel method of utilizing bio-waste combinations produce biomass energy as claimed in claim 1, is characterized in that production engineering bacterium bacterial classification is bacillus fusiformis Clostridium acetobutylicum (ATCC 824).
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