CN103130629A - High-yield water-based curcumin extraction method - Google Patents
High-yield water-based curcumin extraction method Download PDFInfo
- Publication number
- CN103130629A CN103130629A CN2013100889887A CN201310088988A CN103130629A CN 103130629 A CN103130629 A CN 103130629A CN 2013100889887 A CN2013100889887 A CN 2013100889887A CN 201310088988 A CN201310088988 A CN 201310088988A CN 103130629 A CN103130629 A CN 103130629A
- Authority
- CN
- China
- Prior art keywords
- curcumine
- high yield
- water extraction
- yield according
- described step
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a high-yield water-based curcumin extraction method which comprises the following steps: (1) grinding curcuma, soaking with an alkaline water solution of 0-40 DEG C, stirring, extracting, then adding antioxidant, centrifuging, extracting 1-2 times, taking the supernatant, and adding acid to precipitate; and (2) adding an alcohol solution to dissolve the precipitate, centrifuging, adding water to obtain an alcohol dissolving liquid, purifying, concentrating, and drying to obtain curcumin. The curcumin content extracted by the invention can be up to 95%; the extraction and purification yield is more than 80%; and by taking water as extraction solvent, the method is economic, convenient and safe. Thus, the method provided by the invention is a high-yield and low-cost production process.
Description
Technical field
The invention belongs to the preparation field of curcumine, particularly a kind of method of water extraction curcumine of high yield.
Background technology
Turmeric is the per nnial herb of Zingiber turmeric, and rhizome is flourishing, Cheng Cong, and branch ovalize or cylindric contains the number of chemical composition August at florescence, has good pharmaceutical use and economic outlook.Modern pharmacological research shows that one of the main component of turmeric curcumine has cholagogic, anti-inflammatory, reducing blood-fat, the effect such as antibiotic, anticancer.
Cholagogic: these product or its sodium salt have choleretic effect.During intravenous injection, can reduce the content of dog solids component and increase choleresis.But in the cycle, the absolute total amount of cholate, bilirubin, cholesterol excretion increases in its whole choleresis, and it is constant that fatty acid component keeps.Antibiotic: that streptococcus aureus is had stronger antibacterial effect.During oral 5～20mg/kg, can suppress the Guinea pig Gastric infringement that intramuscular injection 20mg/kg5-hydroxy-tryptamine causes.The hepatic injury that Paracetamol is caused has provide protection.
Find in the time of 2004 that curcumine is can Inhibition of HIV-1 integrase active and be used for the clinical trial of acquired immune deficiency syndrome (AIDS).
Anticancer is one of the main pharmacologically active of curcumine, and its effect that suppresses tumour confirmed in many experimentation on animalies repeatedly, and its concrete anticancer mechanism has become the recent research focus.2006, the researchist of NUS discovery, the curcumine in curry contains antioxygenation, can suppress the kind of starch patch and form at brain, thereby lower the chance of suffering from senile dementia.The ratio that this or soluble why Indian suffer from senile dementia is low than the west.In August, 2012, the researchist of U.S. George Mason University discovery, curcumine is the inhibitor of a series of virus infection healthy cells of a kind of prevention of wide spectrum.
Present factory extracts curcumine from turmeric method is mainly organic solvent extraction, and this method is not only brought the dissolvent residual problem to product, has simultaneously the high shortcoming of production cost.Extracting curcumine with water extraction not only reduces production costs greatly, and solved the problem of dissolvent residual in the curcumine, but the extract yield with water extraction extraction curcumine is very low at present, generally all below 10%, the present invention has well solved the problem that yield is low, finished product content is low in the water extraction curcumine.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method of water extraction curcumine of high yield, and the curcumine content that the present invention extracts is up to 95%, and the yield of extraction purifying is more than 80%; The present invention do to extract solvent with water, and economical and convenient is safety again, is a kind of high yield, production technique cheaply.
The method of the water extraction curcumine of a kind of high yield of the present invention comprises:
(1) turmeric is pulverized the rear 0-40 ℃ of alkaline aqueous solution immersion of using, stir and extract, add antioxidant after extraction is completed, centrifugal, repeat 1-2 time, the merging supernatant liquor adds Acid precipitation;
(2) above-mentioned precipitation is added the alcoholic solution dissolving, the centrifugal residue that goes adds water and gets pure lysate, and then purifying, concentrate, and drying namely gets curcumine.
Described step (1) the neutral and alkali aqueous solution is that mass percentage concentration is sodium hydroxide or the potassium hydroxide aqueous solution of 0.5-5%.
In described step (1), the soaking and stirring time is 1-2h.
In described step (1), antioxidant is sodium sulfite solution, and mass percentage concentration is 0.5-1%.
Acid in described step (1) is that concentration of volume percent is aqueous acetic acid or the 1-10% aqueous hydrochloric acid of 20-50%.
In described step (2), alcoholic solution is ethanolic soln, and concentration expressed in percentage by volume is 80%-95%.
In described step (2), the concentration expressed in percentage by volume of pure lysate is 40%-60%.
In described step (2), purification process is the purification with macroreticular resin method.
Described purification with macroreticular resin method is, pure lysate is saturated with resin absorption, will be adsorbed to saturated resin and clean with the alcohol of 40-60%, and removal of impurities is the eluent wash-out with the 65-85% alcoholic solution, the collection elutriant.
Described macroreticular resin is D130.
In described step (2), the crystallization method elutriant is concentrated, and normal temperature is standing, separates out rear collection crystal until crystal.
Beneficial effect
(1) the curcumine content of the present invention's extraction is up to 95%(HPLC), extract the yield of purifying more than 80%;
(2) the present invention makes to extract solvent with water, economical and convenient, and safety is the production technique of a kind of low cost, high yield, high-content, meets energy-saving consumption-reducing production.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
1) curcuma powder 100g, in raw material, turmeric content is 3.43%, the water and the sodium hydroxide 10g that add 10 times of amounts (being 1000ml), 20 ℃ of lower lixiviates 2 hours, adding mass percentage concentration after lixiviate is 0.5% S-WAT, centrifugal, it is that 30% acetum to a large amount of precipitations occur that clear liquid adds concentration expressed in percentage by volume immediately.The gained filter residue adds water and the sodium hydroxide 5g of 10 times of amounts (being 1000ml), 20 ℃ of lower lixiviates 2 hours, adding mass percentage concentration after lixiviate is 0.5% S-WAT, centrifugal, and it is that acetum to a large amount of precipitations of 30% occur that clear liquid adds concentration expressed in percentage by volume immediately.
2) by centrifugal collecting precipitation, merge, be 95% dissolve with ethanol with concentration expressed in percentage by volume, adding suitable quantity of water pure concentration expressed in percentage by volume to the dissolve with ethanol liquid is 50% eventually.
3) dissolve with ethanol liquid is crossed D130 type macroporous resin chromatography column, is 50% washing with alcohol with concentration expressed in percentage by volume, then uses 3 times of column volume 70%(volume fractions) the aqueous ethanolic solution wash-out, collect elutriant.
4) elutriant is concentrated, crystallization, drying namely gets product 2.97g, and curcumine content is 94.7%, and yield is 82%.
Embodiment 2
1) curcuma powder 150g, in raw material, turmeric content is 3.51%, the water and the 25g sodium hydroxide that add 2000ml, 15 ℃ of lower lixiviates 1.5 hours, adding mass percentage concentration after lixiviate is 0.5% S-WAT, centrifugal, it is that 30% acetum to a large amount of precipitations occur that clear liquid adds concentration expressed in percentage by volume immediately.The gained filter residue adds water and the 10g sodium hydroxide of 1500ml, 15 ℃ of lower lixiviates 2 hours, and adding mass percentage concentration after lixiviate is 0.5% S-WAT, centrifugal, it is that acetum to a large amount of precipitations of 30% occur that clear liquid adds concentration expressed in percentage by volume immediately.
2) by centrifugal collecting precipitation, merge, be 80% dissolve with ethanol with concentration expressed in percentage by volume, adding suitable quantity of water pure concentration expressed in percentage by volume to the dissolve with ethanol liquid is 50% eventually.
3) dissolve with ethanol liquid is crossed D130 type macroporous resin chromatography column, is 55% washing with alcohol with concentration expressed in percentage by volume, then uses 3 times of column volume 70%(volume fractions) the aqueous ethanolic solution wash-out, collect elutriant.
4) with the elutriant concentrate drying, namely get product 4.36g, curcumine content is 95.1%, yield is 78.8%.
Embodiment 3
1) curcuma powder 250g, in raw material, turmeric content is 4.01%, adding is 3000ml water and 30g sodium hydroxide, 30 ℃ of lower lixiviates 2 hours, adding mass percentage concentration after lixiviate is 0.8% S-WAT, centrifugal, and it is that acetum to a large amount of precipitations of 50% occur that clear liquid adds concentration expressed in percentage by volume immediately.The gained filter residue adds 2500ml water and 25g sodium hydroxide, 30 ℃ of lower lixiviates 2 hours, and adding mass percentage concentration after lixiviate is 0.8% S-WAT, centrifugal, it is that acetum to a large amount of precipitations of 50% occur that clear liquid adds concentration expressed in percentage by volume immediately.
2) by centrifugal collecting precipitation, merge, be 85% dissolve with ethanol with concentration expressed in percentage by volume, adding suitable quantity of water pure concentration expressed in percentage by volume to the dissolve with ethanol liquid is 50% eventually.
3) dissolve with ethanol liquid is crossed D130 type macroporous resin chromatography column, is 50% washing with alcohol with concentration expressed in percentage by volume, then uses 3 times of column volume 70%(volume fractions) the aqueous ethanolic solution wash-out, collect elutriant.
4) with the elutriant concentrate drying, namely get product 8.63g, curcumine content is 95.8%, yield is 82.4%.
Claims (11)
1. the method for the water extraction curcumine of a high yield comprises:
(1) turmeric is pulverized the rear 0-40 ℃ of alkaline aqueous solution immersion of using, stir and extract, add antioxidant after extraction is completed, centrifugal, repeat 1-2 time, the merging supernatant liquor adds Acid precipitation;
(2) above-mentioned precipitation is added the alcoholic solution dissolving, centrifugal, add water and get pure lysate, then purifying, concentrated, crystallization, drying namely gets curcumine.
2. the method for the water extraction curcumine of a kind of high yield according to claim 1, it is characterized in that: described step (1) the neutral and alkali aqueous solution is that mass percentage concentration is sodium hydroxide or the potassium hydroxide aqueous solution of 0.5-5%.
3. the method for the water extraction curcumine of a kind of high yield according to claim 1 is characterized in that: in described step (1), the soaking and stirring time is 1-2h.
4. the method for the water extraction curcumine of a kind of high yield according to claim 1 is characterized in that: in described step (1), antioxidant is sodium sulfite solution, and mass percentage concentration is 0.5-1%.
5. the method for the water extraction curcumine of a kind of high yield according to claim 1, it is characterized in that: the acid in described step (1) is that concentration of volume percent is aqueous acetic acid or the 1-10% aqueous hydrochloric acid of 20-50%.
6. the method for the water extraction curcumine of a kind of high yield according to claim 1 is characterized in that: in described step (2), alcoholic solution is ethanolic soln, and concentration expressed in percentage by volume is 80%-95%.
7. the method for the water extraction curcumine of a kind of high yield according to claim 1, it is characterized in that: in described step (2), the concentration expressed in percentage by volume of pure lysate is 40%-60%.
8. the method for the water extraction curcumine of a kind of high yield according to claim 1 is characterized in that: in described step (2), purification process is the purification with macroreticular resin method.
9. the method for the water extraction curcumine of a kind of high yield according to claim 8, it is characterized in that: described purification with macroreticular resin method is, the alcohol lysate is saturated with resin absorption, clean being adsorbed to the alcohol of saturated resin with 40-60%, removal of impurities, be the eluent wash-out with the 65-85% alcoholic solution, collect elutriant.
10. the method for the water extraction curcumine of a kind of high yield according to claim 9, it is characterized in that: described macroreticular resin is D130.
11. the method for the water extraction curcumine of a kind of high yield according to claim 1 is characterized in that: in described step (2), the crystallization method elutriant is concentrated, and normal temperature is standing, separates out rear collection crystal until crystal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310088988.7A CN103130629B (en) | 2013-03-19 | 2013-03-19 | A kind of method of water extraction curcumin of high yield |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310088988.7A CN103130629B (en) | 2013-03-19 | 2013-03-19 | A kind of method of water extraction curcumin of high yield |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103130629A true CN103130629A (en) | 2013-06-05 |
| CN103130629B CN103130629B (en) | 2016-05-25 |
Family
ID=48491140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310088988.7A Active CN103130629B (en) | 2013-03-19 | 2013-03-19 | A kind of method of water extraction curcumin of high yield |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103130629B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105341144A (en) * | 2015-10-01 | 2016-02-24 | 常州市奥普泰科光电有限公司 | Preparation method of fruit fresh keeping agent made in manner that silkworm cocoon casings are coated with curcumin |
| CN105360690A (en) * | 2015-11-27 | 2016-03-02 | 全椒县王震家禽养殖专业合作社 | High-nutrient goose fattening feed |
| JP2016199491A (en) * | 2015-04-09 | 2016-12-01 | ハウスウェルネスフーズ株式会社 | Mood state improver |
| CN112521259A (en) * | 2020-12-24 | 2021-03-19 | 晨光生物科技集团股份有限公司 | Preparation method of natural curcumin without vanillin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH069479A (en) * | 1992-06-26 | 1994-01-18 | Kyodo Kumiai Okinawa Pref Gov Kinousei Shokuhin Kaihatsu Center | A method for extracting curcumin from ukon @(3754/24)curcuma longa l.) |
| CN101445440A (en) * | 2008-12-23 | 2009-06-03 | 晨光天然色素集团有限公司 | Method for extracting refined curcumin and curcuma oil from curcuma |
| CN101468944A (en) * | 2007-12-24 | 2009-07-01 | 北京琥珀光华医药科技开发有限公司 | Method for extracting and separating turmeric effective ingredient curcumin |
-
2013
- 2013-03-19 CN CN201310088988.7A patent/CN103130629B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH069479A (en) * | 1992-06-26 | 1994-01-18 | Kyodo Kumiai Okinawa Pref Gov Kinousei Shokuhin Kaihatsu Center | A method for extracting curcumin from ukon @(3754/24)curcuma longa l.) |
| CN101468944A (en) * | 2007-12-24 | 2009-07-01 | 北京琥珀光华医药科技开发有限公司 | Method for extracting and separating turmeric effective ingredient curcumin |
| CN101445440A (en) * | 2008-12-23 | 2009-06-03 | 晨光天然色素集团有限公司 | Method for extracting refined curcumin and curcuma oil from curcuma |
Non-Patent Citations (4)
| Title |
|---|
| 刘新桥: "姜黄中总姜黄素的提取工艺研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技I辑》, 15 September 2004 (2004-09-15), pages 016 - 218 * |
| 宋长生等: "用碱溶液法从姜黄中提取姜黄素的研究", 《精细石油化工进展》, vol. 7, no. 4, 25 April 2006 (2006-04-25), pages 39 - 41 * |
| 曾志丁: "姜黄药材中总姜黄素的提取、精制与分析研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》, 15 April 2012 (2012-04-15), pages 047 - 274 * |
| 袁利佳: "姜黄素静脉注射乳剂的制备", 《中国优秀硕士学位论文全文数据库 工程科技I辑》, 15 October 2007 (2007-10-15), pages 016 - 179 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016199491A (en) * | 2015-04-09 | 2016-12-01 | ハウスウェルネスフーズ株式会社 | Mood state improver |
| CN105341144A (en) * | 2015-10-01 | 2016-02-24 | 常州市奥普泰科光电有限公司 | Preparation method of fruit fresh keeping agent made in manner that silkworm cocoon casings are coated with curcumin |
| CN105360690A (en) * | 2015-11-27 | 2016-03-02 | 全椒县王震家禽养殖专业合作社 | High-nutrient goose fattening feed |
| CN112521259A (en) * | 2020-12-24 | 2021-03-19 | 晨光生物科技集团股份有限公司 | Preparation method of natural curcumin without vanillin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103130629B (en) | 2016-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101671294B (en) | Method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavone from folium mori | |
| CN103130629A (en) | High-yield water-based curcumin extraction method | |
| CN102408415B (en) | Preparation method of mangiferin | |
| CN104496816A (en) | Method for extraction and separation of chlorogenic acid, pinoresinol diglucoside, aucubin and gutta-percha from eucommia ulmodies oliv raw material | |
| CN101862385A (en) | Sanguisorba saponins and preparation method of sanguisorbin I | |
| CN104478888B (en) | A kind of method extracting O methyl 1-hydroxy-2-methoxy-9,10-methylenedioxyaporphine. from Fructus Nandinae Domesticae | |
| CN101475570B (en) | Method for extracting hypotensor raw material alserin from davilpepper | |
| CN102002083B (en) | Method for extracting high-purity rutin with flash-extraction technology | |
| CN102070684A (en) | Method for extracting syringin | |
| CN109705127B (en) | Anti-emulsification preparation method of plant-derived sodium copper chlorophyllin | |
| JP2012501990A5 (en) | ||
| CN104292366A (en) | Method for simultaneously extracting arabinogalactan and dihydroquercetin from dahurian larch | |
| CN101759731B (en) | Extraction method of linseed gum and secoisolariciresin-ol diglucoside | |
| CN103421058B (en) | A kind of method of high-level efficiency clean cut separation purifying Rhapontin, deoxy- | |
| CN101434590B (en) | Preparation of salvianolic acid B pure product | |
| CN102504007A (en) | Method for separation and purification of ruscogenin monomer | |
| CN102329209A (en) | Method for extracting emodin from giant knotweed rhizome | |
| CN103012072A (en) | Technology for preparing high-purity honokiol and magnolol | |
| CN102372634A (en) | Preparation method for cynarin | |
| CN102816065A (en) | Use of artemisia annua and artemisia annua industrial-extraction residues as raw materials for caffeoylquinic acid preparation | |
| CN103044410A (en) | Production process for extracting isoorientin from bamboo product waste | |
| CN102631536B (en) | Extraction process of alkaloid in thunberg fritillary bulb | |
| CN101973973A (en) | Method for extracting usnic acid from sun-lo | |
| CN101912438A (en) | Extraction technology of total alkaloids of sophora alopecuroides | |
| CN104622999A (en) | Process for extracting total triterpenes from silverweed cinquefoil roots |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 315806 Zhejiang city of Ningbo province Beilun Daqi Gold Road No. 525 Applicant after: NINGBO CHINESE MEDICINE PHARMACEUTICAL CO., LTD. Address before: 315806 Zhejiang city of Ningbo province Beilun Daqi Gold Road No. 525 Applicant before: Ningbo Traditional Chinese Pharmaceutical Co., Ltd. |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |