CN103127319A - Lipid lowering function of eleutherine plicata herb and extract thereof - Google Patents
Lipid lowering function of eleutherine plicata herb and extract thereof Download PDFInfo
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- CN103127319A CN103127319A CN2013100712571A CN201310071257A CN103127319A CN 103127319 A CN103127319 A CN 103127319A CN 2013100712571 A CN2013100712571 A CN 2013100712571A CN 201310071257 A CN201310071257 A CN 201310071257A CN 103127319 A CN103127319 A CN 103127319A
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Abstract
The invention discloses new functions of eleutherine plicata herb and an extract thereof, including reducing blood lipid and preventing hyperlipemia. The function of reducing blood lipid refers to reducing contents of serum cholesterol, triglyceride and low density lipoprotein cholesterin and improving content of high density lipoprotein cholesterin; and the function of preventing hyperlipemia refers to preventing high serum cholesterol, high triglyceride, high content of low density lipoprotein cholesterin, and low content of high density lipoprotein cholesterin.
Description
Technical field
The present invention relates to the new role of a kind of plant, is the new role of Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae and extract thereof specifically, belongs to the field of Chinese medicines.
Background technology
Bulbus Eleutherines Plicatae is the herb of irides Bulbus Eleutherines Plicatae Eleutheri ne pl icata Herb., originates in the West Indies, is distributed in the ground such as Guangxi, Yunnan in China, is the Medicinal plant." China's book on Chinese herbal medicine " carries it the effect of clearing away heat and cooling blood, promoting blood circulation to restore menstrual flow, subduing swelling and detoxicating.Cure mainly haematemesis, spitting of blood, dysentery, amenorrhea stomachache, rheumatic arthralgia, traumatic injury, furuncle toxic swelling.Book on Chinese herbal medicine among the people carries it and can be used for menorrhagia, red collapsing, epistaxis, gastrointestinal hemorrhage, traumatic hemorrhage, dysentery, rheumatic arthritis, treating swelling and pain by traumatic injury, sore; The doctor of the Dai Nationality is used for knife injury with bulb, epilepsy clonus, and arthralgia, dizziness, nervous, vomiting uncomfortable in chest, general fatigue is unable.In conjunction with the local utilization among the people in Guangxi, the efficacy effect of summarizing Bulbus Eleutherines Plicatae concentrates on 1. gynaecopathia: amenorrhea stomachache, menorrhagia; 2. gastroenteropathy: gastrointestinal hemorrhage, dysentery; 3. arthropathy: rheumatic arthritis, treating swelling and pain by traumatic injury.According to modern pharmacology research, Bulbus Eleutherines Plicatae have blood fat reducing, blood sugar lowering, antioxidation and antimutagenic, antibiotic, increase coronary artery blood flow, suppress HIV, suppress the multiple pharmacologically actives such as human body topoisomerase II.But still do not find the research report of Bulbus Eleutherines Plicatae aspect blood fat reducing at present.
Summary of the invention
The object of the invention is to disclose the new role of a kind of Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae and extract thereof.
The present invention realizes effect research of experimental hyperlipemia in rats by Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae and extract thereof.
Method: the SD rat of SPF level is divided into Normal group and model group at random, rat model gave fed with high in the time of 7 days, be divided at random Bulbus Eleutherines Plicatae extract group (1.3g/Kg/d), fenofibrate group (20.6mg/Kg/d) and model control group (isometric(al) normal saline), and continue to raise with high fat foodstuff, gavage gives said medicine processing 30 days simultaneously, after last administration 50min, animal is at 3% pentobarbital sodium (30mg/kg, ip) under anesthesia, abdominal aortic blood, precipitation is extracted supernatant and is carried out lipid determination.
Result: the Bulbus Eleutherines Plicatae extract obviously reduces the level (p＜0.05) of cholesterolemia ester (p＜0.01), triglyceride and the low density lipoprotein, LDL of rat model, improves hdl concentration, and it transfers the fat effect to be better than fenofibrate.
Conclusion: Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae extract has the effect of obvious accent fat to this Hyperlipemia model rat.
1. experiment material
1.1 animal: SPF level SD rat, male 55, body weight 200 ± 20 (g), Nanfang Medical Univ's Experimental Animal Center provide (credit number: scxk Guangdong 2011-0015, NO:0115045).
1.2 medicine: Bulbus Eleutherines Plicatae granule (5g/ bag), Guangzhou Lanyun Medicine Research Co., Ltd.'s (lot number: 120601); Fenofibrate (sharp purport is flat), strong Pharmaceutical limited company of Guangdong elder generation (lot number: 110401).
1.3 animal feeding situation: common rat material and high lipid food provide (processing numbering: 20120925C by Guangdong Medical Lab Animal Center; Formula: (78.8% normal diet, 1% cholesterol, 10% yolk powder, 10% Adeps Sus domestica, 0.2% cholate).After animal is bought, raised 5 days at this laboratory adaptability, free drinking water, lamp was according to 12 hours.
1.4 instrument and equipment: full automatic biochemical apparatus (Hitachi 7600).
2. experimental technique
2.1 animal grouping, modeling and drug treating: 55 male rats are divided into 4 groups at random, normal rat matched group (15) and model group (40); Model group gives fed with high every day, during free drinking water to 7 day, measure the Some Animals blood fat, if blood fat has increasing to a certain degree, both whole animal patterns can be divided into Bulbus Eleutherines Plicatae group (1.3g/Kg/d, 15), (15 of model control group, the isometric(al) normal saline) and fenofibrate group (20.6mg/Kg/d, 10); After grouping, continue to feeding with high lipid food, gavage gives said medicine respectively simultaneously, and the model group rat gives corresponding normal saline, every day 1 time, continuous 30 days.
2.2 lipids detection: after rat oral gavage administration 50min, animal is at 3% pentobarbital sodium (30mg/kg, ip) under anesthesia, abdominal aortic blood, precipitation, get supernatant after centrifugal, carry out blood fat (triglyceride, T-CHOL, high density lipoprotein, low density lipoprotein, LDL etc.) in overseas Chinese's hospital laboratory and detect.
2.3 statistical procedures: all data all with
Expression adopts the spss16.0 statistical software to process, and each is organized group difference and relatively adopts the t method of inspection, and P＜0.05 has statistical significance for difference.
3. experimental result
Model group, normal group, Bulbus Eleutherines Plicatae extract group, fenofibrate group see Table 1 and Fig. 1, Fig. 2, Fig. 3 and Fig. 4 to the blood fat of hyperlipidemia rats.By table 1 and Fig. 1～4 as seen, the content of rat model cholesteryl ester and low density lipoprotein, LDL is apparently higher than normal rat (p＜0.05), increases and high density lipoprotein slightly reduces and triglyceride is slight; After Bulbus Eleutherines Plicatae extract-treated rat model, obviously lower the content (p＜0.05) of cholesteryl ester (Chol) (p＜0.01), triglyceride (TG) level (p＜0.05) and low density lipoprotein, LDL (LDL-C), (HDL-C) has slight increasing action to high density lipoprotein; After fenofibrate transaction module animal, cholesteryl ester, triglyceride levels and low density lipoprotein, LDL content all have slight downward effect, but have no significant difference (p＞0.05).
The impact of table 1 Bulbus Eleutherines Plicatae extract on hyperlipidemia rats blood lipid metabolism
Annotate: compare with model group:
aQ＜0.05,
bP＜0.01.
This shows, the Bulbus Eleutherines Plicatae extract has the effect of obvious accent fat to this Hyperlipemia model rat.
The present invention tests and uses the Bulbus Eleutherines Plicatae extract, employing Bulbus Eleutherines Plicatae herb ethanol extraction, concentrated rear acquisition, test the main component that has kept Bulbus Eleutherines Plicatae with the Bulbus Eleutherines Plicatae extract, the invention belongs to the basic research of Bulbus Eleutherines Plicatae new role.Bulbus Eleutherines Plicatae can be made various functional food or medicines with effect for reducing blood fat.
Description of drawings
Fig. 1 is that the Bulbus Eleutherines Plicatae extract is on the figure that affects of hyperlipidemia rats serum cholesterol (Chol).When wherein a refers to model group relatively, use when p＜0.05; When b refers to model group relatively, use when p＜0.01.
Fig. 2 is that the Bulbus Eleutherines Plicatae extract is on the figure that affects of hyperlipidemia rats serum triglycerides (TG).When wherein a refers to model group relatively, use when p＜0.05.
Fig. 3 is that the Bulbus Eleutherines Plicatae extract is on the figure that affects of hyperlipidemia rats serum high-density LP (HDL-C).
Fig. 4 is that the Bulbus Eleutherines Plicatae extract is on the figure that affects of hyperlipidemia rats serum low-density LP (LDL-C).When wherein a refers to model group relatively, use when p＜0.05.
The specific embodiment
Below by embodiment, the present invention is described in further details, these embodiment only are used for illustrating the present invention, do not limit the scope of the invention.
Embodiment 1: adopt following method to prepare Bulbus Eleutherines Plicatae extract of the present invention:
Get Bulbus Eleutherines Plicatae 1000g, add 70% the ethanol extraction 2 times of 8 times of amounts, each 1h.Extracting liquid filtering, merging filtrate, filtrate recycling ethanol gets extractum.Extractum is concentrated to the thick paste that relative density is 1.30-1.40 under 60 ℃, at 60～70 ℃, vacuum drying under the condition of-0.08mpa is pulverized, and namely gets the Bulbus Eleutherines Plicatae extract.
Described Bulbus Eleutherines Plicatae extract can be used for being prepared into functional food or the medicine with effect for reducing blood fat.
Embodiment 2: the Bulbus Eleutherines Plicatae granule
With the Bulbus Eleutherines Plicatae extract, with maltodextrin, micropowder silica gel, steviosin mixing, add 95% appropriate ethanol to make soft material, granulate with 14 mesh sieves, drying, granulate is prepared into granule.
Embodiment 3: the Bulbus Eleutherines Plicatae sheet
With the Bulbus Eleutherines Plicatae extract, with low-substituted hydroxypropyl methylcellulose, magnesium stearate mix homogeneously, add 95% alcoholic solution and make in right amount soft material, cross 14 mesh sieves, drying, granulate adds microcrystalline Cellulose, mixing, tabletting namely gets tablet.
Embodiment 4: the Bulbus Eleutherines Plicatae capsule
With the Bulbus Eleutherines Plicatae extract, with hydroxypropyl cellulose, Pulvis Talci, microcrystalline Cellulose, calcium sulfate mixing, add 95% appropriate ethanol to make soft material, granulate with 14 mesh sieves, drying, granulate incapsulates and get final product.
Embodiment 5: the Bulbus Eleutherines Plicatae soft capsule
Edible vegetable oil and the Cera Flava of Bulbus Eleutherines Plicatae extract with process heat sterilization, clarification mixed, fully stir into even, stable solution.Get gelatin, glycerol, PGE400, add suitable quantity of water to heat under 70 ℃ and be mixed to the gelatin dissolving, get capsule shell fluid.Adopt inferior magic to be prepared into soft capsule the capsule shell fluid for preparing and content solution, at 25-35 ℃, under the condition of relative humidity 20-45% vacuum drying 10-30 hour, and get final product.
Claims (5)
1. Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae and extract thereof have the effect of blood fat reducing.
2. effect as claimed in claim 1, wherein effect for reducing blood fat refers to reduce serum cholesterol, reduces triglyceride, reduces the content of low-density lipoprotein cholesterol or the content of high density lipoprotein increasing cholesterol.
3. Iridaceae Bulbus Eleutherines Plicatae platymiscium Bulbus Eleutherines Plicatae and extract thereof have the effect of prevention hyperlipemia.
4. effect as claimed in claim 3 wherein prevents hyperlipemia to refer to the rising of prophylactic serum cholesterol, and the rising of prevention TG prevents the rising of low-density lipoprotein cholesterol content or the reduction of prevention HDL-C content.
5. effect described according to claims 1 or 3 is characterized in that described effect is functional food or the medicine that Bulbus Eleutherines Plicatae or Bulbus Eleutherines Plicatae extract and pharmaceutically acceptable carrier or diluent are prepared into the dosage forms such as granule, tablet, capsule, oral liquid, slow releasing preparation, controlled release preparation, pill, suspensoid, powder, injection.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104818311A (en) * | 2015-05-13 | 2015-08-05 | 武昌理工学院 | Preparation method and application of onion active polypeptide extract |
| CN105076599A (en) * | 2015-07-15 | 2015-11-25 | 符健 | Tea bag containing American eleutherine rhizomes and capable of improving immunity of human bodies and preparation method of tea bag |
| CN113855748A (en) * | 2021-11-10 | 2021-12-31 | 四川化工职业技术学院 | Composite plant source extract and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331282A (en) * | 2000-07-03 | 2002-01-16 | 邢万善 | Sancong wine |
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2013
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331282A (en) * | 2000-07-03 | 2002-01-16 | 邢万善 | Sancong wine |
Non-Patent Citations (2)
| Title |
|---|
| 海南人民医院冠心病科研小组: "海冠I号（红葱）治疗冠状动脉粥样硬化性心脏病30例临床观察", 《海南医学》, no. 2, 31 December 1977 (1977-12-31) * |
| 田建华: "《高血脂调养食谱与生活护理》", 31 October 2006, article "洋葱" * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104818311A (en) * | 2015-05-13 | 2015-08-05 | 武昌理工学院 | Preparation method and application of onion active polypeptide extract |
| CN104818311B (en) * | 2015-05-13 | 2017-12-01 | 武昌理工学院 | A kind of preparation method and application of onion active polypeptide extract |
| CN105076599A (en) * | 2015-07-15 | 2015-11-25 | 符健 | Tea bag containing American eleutherine rhizomes and capable of improving immunity of human bodies and preparation method of tea bag |
| CN113855748A (en) * | 2021-11-10 | 2021-12-31 | 四川化工职业技术学院 | Composite plant source extract and preparation method and application thereof |
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Application publication date: 20130605 |