CN103105670A - Cell slide glass for microscopic observation - Google Patents
Cell slide glass for microscopic observation Download PDFInfo
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- CN103105670A CN103105670A CN2013100471880A CN201310047188A CN103105670A CN 103105670 A CN103105670 A CN 103105670A CN 2013100471880 A CN2013100471880 A CN 2013100471880A CN 201310047188 A CN201310047188 A CN 201310047188A CN 103105670 A CN103105670 A CN 103105670A
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- slide
- cell
- glass
- microscopic observation
- plastic
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Abstract
The invention discloses a cell slide glass for microscopic observation. The cell slide glass for microscopic observation comprises a glass slide (1) and is characterized in that a plastic slide (2), a cellular layer (3) and a glass cover slide (4) are arranged on the glass slide (1) from bottom to top through adhesive layers (5). The cell slide glass for microscopic observation is capable of taking advantages of the plastic slide as a cell culture slide, and influences on cell observation results through microscopic observation caused by high turbidity, high birefringence and the like of the plastic slide can be eliminated, and experimental results are prevented from being avoided by abrasion in a storage process due to the fact that the glass cover slide is abrasion-proof.
Description
Technical field
The present invention relates to a kind of Microscopic observation cell slide glass, especially a kind of Microscopic observation cell slide glass of avoiding cellular morphology to change, guarantee experiment effect.
Background technology
Along with constantly enriching of life science content is abundant, the technical indicator of utilizing the cell culture experiments in vitro sample to analyze and observe also is on the increase, the field of containing enlarges increasingly, and is therefore at medical domain, very general with the surface topography of microscope observing cell.For the ease of preserving and observation of cell, avoid cellular morphology to change, usually cell climbing sheet need to be enclosed on microslide admittedly with glue-line.Preparation method in the past be get some suitable sizes (as the glass cover slide of 10mm * 20mm, thickness 0.14. ~ 0.17mm), pickling and fully rinse after autoclave sterilization; The glass cover slide is put into double dish/plate, before conventional cell is laid on sheet; , after substantially covering with on the glass cover slide cell climbing sheet is taken out when cell; Use PBS rinsing 3 times under 4 ℃, fully remove remaining nutrient culture media and serum, cell faces up and puts into 4 ℃ of cold acetones and fix 20 minutes, dries, the cellular layer that will be covered with afterwards cell climbing sheet is fastened on microslide down, then fixes, seals (Gu envelope) with glue-lines such as resins.Because the cell attachment of glass cover slide is poor, for improving cell attachment, must be coated with processing to the glass cover slide before cultivating, waste time and energy; The glass cover slide increases through fragility after repeatedly dehydration and drying are processed, and easy fracture causes the variation of cellular morphology, especially can cause having the cell dendron fracture of longer dendron, causes the attached cell shrinkage when serious, subsides; Each glass cover slide that all needs to be covered with cellular layer takes out from Tissue Culture Dish, because the glass cover slide is large with the adhesive force of double dish bottom surface, therefore it is comparatively difficult to take out microslide, and easy generation cut etc. on the cell face.For this reason, people have substituted glass slide with plastic slide.
Plastic slide has following advantage: can become hydrophilic surface to promote that cell adheres to; Non-chlorinated hydrocarbon, acetone and other Electronic Speculum are not all reacted with reagent; Can directly fix, carry out staining reaction etc. after becoming cell climbing sheet; Can directly cut to needed shape etc. with scissors.But the optical signature due to plastic slide itself, the phenomenons such as the high and birefringence of turbidity is large can appear, the turbidity height can make the mirror hypograph unintelligible, and the more large image that more easily causes of birefringence produces the phenomenons such as askew shadow, all affects microscopically to the observation of cell experiment result.Plastic slide itself is not wear-resisting simultaneously, and in storage process, its outside surface easily weares and teares, and further affects observing effect.
Summary of the invention
The present invention is in order to solve the existing above-mentioned technical matters of prior art, a kind of Microscopic observation cell slide glass of avoiding cellular morphology to change, guarantee experiment effect to be provided.
Technical solution of the present invention is: a kind of Microscopic observation cell slide glass, microslide is arranged, and be sealed with successively plastic slide, cellular layer and glass cover slide from bottom to top by glue-line on microslide.
The present invention is that the cellular layer of the plastic slide (cell climbing sheet) that will be covered with cellular layer is fixed on microslide up, and then the glass cover slide is fixed on cellular layer, both can bring into play plastic slide and cultivate the various advantages of creep plate as cell, can eliminate again that plastic slide is high because of turbidity, birefringence is large etc. to the impact of microscopically observation of cell result, also can avoid being worn in storage process because of the anti-wear performance of glass cover slide and affect experimental result.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the present invention.
Embodiment
Below in conjunction with description of drawings the specific embodiment of the present invention.As shown in Figure 1: microslide 1 is arranged as the prior art, is to be sealed with successively plastic slide 2, cellular layer 3 and glass cover slide 4 from bottom to top by glue-line 5 on microslide 1 with the prior art difference.Concrete preparation method is: cultivate as the prior art creep plate take plastic slide 2 as cell, take out after covering with cell on it, make the dry plastic slide that is covered with cellular layer 32 according to art methods, cellular layer 3 plastic slide 2 up is placed on microslide 1, again glass cover slide 4 is pressed on cellular layer 3, use at last glue-line 5(resin) fixing seal, get final product.
During microscopic examination, can pass through the direct observation of cell layer 3 of glass cover slide 4, avoid plastic slide 2 on the impact of observations.
Claims (1)
1. a Microscopic observation cell slide glass, have microslide (1), it is characterized in that: upward be sealed with successively plastic slide (2), cellular layer (3) and glass cover slide (4) from bottom to top by glue-line (5) at microslide (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100471880A CN103105670A (en) | 2013-02-06 | 2013-02-06 | Cell slide glass for microscopic observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100471880A CN103105670A (en) | 2013-02-06 | 2013-02-06 | Cell slide glass for microscopic observation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103105670A true CN103105670A (en) | 2013-05-15 |
Family
ID=48313653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100471880A Pending CN103105670A (en) | 2013-02-06 | 2013-02-06 | Cell slide glass for microscopic observation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103105670A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002006880A2 (en) * | 2000-07-17 | 2002-01-24 | Veracel Inc. | Plastic substrates slide apparatus for infrared microscopy analysis |
| WO2007138568A2 (en) * | 2006-05-25 | 2007-12-06 | Yeda Research And Development Co. Ltd. | A device for microscopy flow-through experiments on non-adherent live cells |
| CN203164506U (en) * | 2013-02-06 | 2013-08-28 | 李宏 | Cell slide glass for microscopic observation |
-
2013
- 2013-02-06 CN CN2013100471880A patent/CN103105670A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002006880A2 (en) * | 2000-07-17 | 2002-01-24 | Veracel Inc. | Plastic substrates slide apparatus for infrared microscopy analysis |
| WO2007138568A2 (en) * | 2006-05-25 | 2007-12-06 | Yeda Research And Development Co. Ltd. | A device for microscopy flow-through experiments on non-adherent live cells |
| CN203164506U (en) * | 2013-02-06 | 2013-08-28 | 李宏 | Cell slide glass for microscopic observation |
Non-Patent Citations (1)
| Title |
|---|
| 周长信: "用改进的载玻片做装片观察水螅", 《生物学通报》 * |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130515 |