CN103103233A - Method for preparing chitosan by xylose in hemicellulose hydrolysate - Google Patents
Method for preparing chitosan by xylose in hemicellulose hydrolysate Download PDFInfo
- Publication number
- CN103103233A CN103103233A CN2013100278493A CN201310027849A CN103103233A CN 103103233 A CN103103233 A CN 103103233A CN 2013100278493 A CN2013100278493 A CN 2013100278493A CN 201310027849 A CN201310027849 A CN 201310027849A CN 103103233 A CN103103233 A CN 103103233A
- Authority
- CN
- China
- Prior art keywords
- hemicellulose hydrolysate
- hemicellulose
- chitosan
- wood sugar
- mycelium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to a method for preparing chitosan by xylose in hemicellulose hydrolysate. The method comprises the following steps of: (1) after smashing a lignocellulose material and performing acid hydrolysis for the hemicellulose; (2) detoxifying and decoloring the hemicellulose hydrolysate; (3) culturing filamentous fungi by using detoxified hemicellulose hydrolysate as a carbon source; (4) removing binding proteins of the filamentous fungi by an alkali liquor; and (5) extracting chitosan from filamentous fungi by a weak acid. According to the method provided by the invention, the hemicellulose hydrolysate is detoxified and decolored by Ca(OH)2 and active carbon, and poisonous components (such as formic acid, formic acid and furfural) in the treated hemicellulose hydrolysate are remarkably reduced, so that a good growing environment is provided for mycelium. Therefore, hemicellulose in lignocellulose is utilized and converted, and the yield of chitosan is improved. The method is simple and convenient to operate.
Description
Technical field
The present invention relates to biomass and utilize technical field, especially a kind of method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan.
Background technology
Chitosan is the natural alkaline polysaccharide of unique a large amount of existence of finding up to now, has unique character and physiological function, as permeability, fiberizability, water absorbability, tackifying, film-forming properties, adsorptivity, germ resistance, biocompatibility, moisture retention etc.Be widely used in the fields such as environmental protection, textile printing and dyeing, food, chemical industry, makeup, water treatment and biological medicine in recent years.The production method of chitosan is main at present still extracts with soda acid processing from shrimp and crab shells, and this process need expend a large amount of soda acids, and corrodibility is strong, and waste liquid amount is large, easily causes environmental pollution; When this external application concentrated base reacted, chitinous molecule was easily degraded, and molecular weight and viscosity is reduced and affect the quality of product.Chitosan is also the important composition composition of some fungal cell wall, and preparing on a large scale chitosan with biotechnology cultivation filamentous fungus may become coming cleaner production mode.
Lignocellulose is renewable resources the abundantest on the earth, mainly by three kinds of forms polymkeric substance---Mierocrystalline cellulose, hemicellulose and xylogen form.Nowadays, along with the minimizing day by day of petroleum resources, the raising of oil production and tooling cost, and the public and social more and more higher to the requirement of environmental quality utilize lignocellulosic materials for fuel ethanol to become study hotspot.At present, confirm to utilize Mierocrystalline cellulose can produce various types of biofuels (as ethanol, 2,3-butanediol etc.), yet utilized biomass material scale operation alcohol fuel to remain a huge challenge.Hemicellulose is one of important component of biomass, can be hydrolyzed into relatively easily wood sugar, yet the difficult point of wood sugar zymamsis is that microorganism is difficult to utilize wood-sugar fermentation ethanol.Although some yeast (as pichia yeast etc.) and genetic engineering bacterium (as through the intestinal bacteria of transformation etc.) can utilize xylose production ethanol, also have larger distance from practical application.Therefore, in cellulosic ethanol production, a large amount of hemicelluloses are wasted.Many microorganisms can utilize wood sugar to change into other products, and this has great importance to taking full advantage of of lignocellulose.
By retrieval, not yet find the patent publication us relevant to patent application of the present invention.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, the wood sugar in hemicellulose hydrolysate of utilizing that provides that a kind of method is simple, easy to operate, productive rate is high prepares the method for chitosan.
The present invention solves its technical problem and is achieved through the following technical solutions:
A kind of method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan, step is as follows:
⑴ hemicellulose raw material hydrolysis: lignocellulosic material is pulverized, pressed lignocellulosic material: H
2SO
4The solid-to-liquid ratio g:mL of solution is that to add volume fraction be 0.5～2.0% H to the ratio of 1:8 ~ 11
2SO
4Solution at 100～120 ℃ of hydrolysis 1～3h, gets hemicellulose hydrolysate;
⑵ detoxification decolouring is processed: add Ca (OH) in hemicellulose hydrolysate
2Transfer pH to 9～11, the supernatant liquor after solid-liquid separation is transferred pH to 6～8, is then that 1:20～50 add gac by solid-to-liquid ratio g:mL, is incubated 0.5～2.0h under 50 ℃ and carries out the detoxification decolouring and process;
⑶ the cultivation of filamentous fungus: add corn steep liquor, KH in the hemicellulose hydrolysate after processing to the detoxification decolouring
2PO
4And MgSO
47H
2O makes fermention medium, after access filamentous fungus spore suspension, gets fermented liquid through fermentation culture in the fermention medium;
Wherein, hemicellulose hydrolysate: corn steep liquor: KH
2PO
4: MgSO
47H
2The ratio mL:g:g:g of O is 100:4.0～7.0:0.1～0.6:0.1～0.6;
⑷ mycelium deproteinated: with filtering fermentation liquor, obtain mycelium, to grind after the mycelium oven dry, according to mycelium: the ratio g:mL of alkali lye is that to add massfraction be 2～6% alkali lye to the ratio of 1:20～60, process 2～4h for 90 ~ 110 ℃, solid-liquid separation obtains the alkali insolubles, is washed till filtrate neutrality;
⑸ chitosan extracts: in the alkali insolubles that is washed till filtrate neutrality according to mycelium: the ratio g:mL of acid solution is that to add volume fraction be 2～6% weakly acid soln in 1:20～60, process 2～6h in 50～70 ℃, solid-liquid separation, get supernatant liquor, adjust pH is 9～12, standing 5～15min, the collecting precipitation thing also washs to filtrate as neutral, solid-liquid separation, 40～60 ℃ of oven dry namely get chitosan.
And in described step ⑴, lignocellulosic material sieves after crushed and makes granular size be no more than 1mm.
And in described step ⑴, lignocellulosic material is one or more the mixture in maize straw, corn cob, straw, wheat straw.
And transferring the solution that o'clock uses pH to 6～8 in described step ⑵ is 10% H as volume fraction
2SO
4Or H
3PO
4Solution.
And in described step ⑶, access filamentous fungus spore suspension is to be 10 with spore suspension concentration
6～10
8mL
– 1The filamentous fungus spore suspension access by inoculum size 4～8%.
And in described step ⑶, fermentation culture conditions is: initial pH 6～7,26～34 ℃ of culture temperature, mixing speed 50～200r/min, air flow 0.5～2vvm, inoculum size 4～8%, incubation time 48～64h.
And in described step ⑶, filamentous fungus is graceful actinomucor TCCC450001(Actinomucor elegans).
And in described step ⑷, alkali lye is NaOH solution.
And, when transferring pH, uses described step ⑸ NaOH solution; Described weak acid is acetic acid.
And described chitosan also passes through following processing: with 95% washing with alcohol 2 ~ 4 times, then use washing with acetone 1 ~ 3 time, 40～60 ℃ of oven dry.
The advantage that the present invention obtains and positively effect are:
1, the inventive method is utilized Ca (OH)
2With activated carbon, hemicellulose hydrolysate being carried out the detoxification decolouring processes, hemicellulose hydrolysate toxic composition (as formic acid, acetic acid, furfural etc.) after processing obviously reduces, for mycelium provides good growing environment, realized utilization and the conversion of hemicellulose in the lignocellulose, improved the productive rate of chitosan, method is simple, easy to operate.
2, the inventive method is take lignocellulose as raw material, hemicellulose is through after acid hydrolysis, hemicellulose hydrolysate after the detoxification is cultivated filamentous fungus as carbon source, hemicellulose is utilized by filamentous fungus, finally change into the chitosan of high added value, for taking full advantage of of lignocellulose component provides sufficient theoretical foundation, significant to taking full advantage of of lignocellulose simultaneously, have broad application prospects in fields such as medicine, food, chemical industry.
3, the stalk of the inventive method in the agricultural wastes is as raw material, hemicellulose in raw material is converted into the chitosan product with high added value, both reduce the environmental pollution that causes due to a large amount of burn processing of stalk, and provided a kind of new method for microbial method prepares chitosan.
Description of drawings
Fig. 1 is the fermenting process Dynamic Graph of graceful actinomucor when fermentation culture of the embodiment of the present invention 1.
Embodiment
The invention will be further described below by specific embodiment.Following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
The method of using in the present invention is ordinary method if no special instructions; The reagent that uses in the present invention is conventional reagent if no special instructions.
The deposit number of the graceful actinomucor of the preservation of bacteria strain that uses in the present invention is TCCC450001(Actinomucor elegans), at present, this bacterial strain is by as open in Publication about Document: Liu Zhenli, Zhao Hua, Chu Ning. substratum forms the impact [J] on graceful actinomucor fermentative production chitosan. University Of Science and Technology Of Tianjin's journal, 2010,25 (2): 13-17.
The lignocellulosic material that uses in the present invention can be one or more the mixture in maize straw, corn cob, straw, wheat straw.
Embodiment 1
A kind of method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan, step is as follows:
⑴ the hydrolysis of hemicellulose raw material: corn cob sieves after crushed and makes granular size be no more than 1mm; Be 1.0% H with the corn cob volume fraction after pulverizing
2SO
4Solution is pressed corn cob: H
2SO
4The solid-to-liquid ratio g:mL of solution is 1:10 120 ℃ of hydrolysis 2h in hydrolyzer, gets hemicellulose hydrolysate;
⑵ detoxification decolouring is processed: add Ca (OH) in the hemicellulose hydrolysate that obtains in step ⑴
2Transfer pH to 10, get supernatant liquor after solid-liquid separation, the supernatant liquor volume fraction is 10% H
2SO
4Solution is transferred pH to 7, and solid-liquid separation gets supernatant liquor; Again to through Ca (OH)
2In supernatant liquor after detoxification treatment by solid-to-liquid ratio 1:20(g:mL) add gac, be incubated 1h under 50 ℃, obtain the hemicellulose hydrolysate after the detoxification decolouring is processed after filtration;
After testing, obtain after filtration that in hemicellulose hydrolysate after detoxification decolouring is processed, Xylose Content is 29.33g/L;
⑶ filamentous fungus is cultivated:
1. strain inclined plane is cultivated: adopting the PDA substratum is slant medium, and graceful actinomucor thalline is seeded on slant medium, cultivates 4d for 28 ℃ in constant incubator, takes out after growing a large amount of spores on the inclined-plane;
2. spore suspension preparation: with the physiological saline 10mL wash-out spore of the bacterium of going out, two-layer sterile gauze removes by filter mycelium, filtrate is fully vibrated obtains spore suspension, and with the blood counting chamber counting, making ultimate density with the spore in distilled water adjustment spore suspension is 10
6mL
– 1
3. the fermentation culture of graceful actinomucor TCCC450001: add corn steep liquor 7.0%(corn steep liquor in the hemicellulose hydrolysate after the detoxification decolouring is processed: hemicellulose hydrolysate, g:mL), KH
2PO
40.2%(KH
2PO
4: hemicellulose hydrolysate, g:mL) and MgSO
47H
2O 0.2%(MgSO
47H
2O: hemicellulose hydrolysate, g:mL), make fermention medium;
Add the 3L fermention medium in the 5L fermentor tank, with massfraction be 30% NaOH solution to regulate initial pH be 6.5, (spore concentration is 10 to access 180mL spore suspension in the fermention medium
6mL
– 1), control 30 ℃ of culture temperature, mixing speed 100r/min, air flow 1.5vvm cultivates 52h, obtains fermented liquid;
⑷ mycelium deproteinated: with filtering fermentation liquor, obtain mycelium.Mycelium is ground after 24h in 65 ℃ of bakings, with massfraction be 2% NaOH solution (mycelium: alkali lye=1:20, g:mL) 100 ℃ of processing 2h, solid-liquid separation obtains the alkali insolubles, is washed till filtrate neutrality with distilled water;
⑸ chitosan extracts: the alkali insolubles volume fraction that will be washed till filtrate neutrality is 2% acetum (mycelium: acid solution=1:40, g:mL) process 3h in 60 ℃, solid-liquid separation gets supernatant liquor, slowly adding massfraction in the supernatant liquor is 30% NaOH solution, and the limit edged stirs, adjust pH to 9, a large amount of white flockss appear, collecting precipitation thing and be washed with distilled water to filtrate for neutral after standing 5min, 50 ℃ of oven dry are the chitosan crude product;
⑹ use 95% ethanol with the chitosan crude product again, and (chitosan: 95% ethanol=1:20, g:mL) washing is 3 times, and (chitosan: acetone=1:20, g:mL) washing is 2 times, and 50 ℃ of oven dry can obtain the chitosan sterling to use at last acetone.
After testing, the productive rate of chitosan is 2.87g/L.
Accompanying drawing 1 is the fermenting process Dynamic Graph of embodiment 1.As shown in Figure 1, in the whole fermenting process of graceful actinomucor, the pH rear reduction that first raises in fermented liquid, but entire change is little, xylose and glucose during beginning in fermented liquid takes the lead in being utilized by mycelium, and glucose almost is utilized fully at 20h, and mycelium just begins to utilize pectinose at 32h.Initial stage, mycelium is slowly grown, and enters logarithmic phase when 24h, and 52h tends towards stability.As seen from the above, the inventive method is take lignocellulose as raw material, and hemicellulose is through after acid hydrolysis, and the hemicellulose hydrolysate after detoxification is cultivated filamentous fungus as carbon source, and hemicellulose is utilized by filamentous fungus, finally changes into the chitosan of high added value.
A kind of method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan, step is as follows:
⑴ the hydrolysis of hemicellulose raw material: corn cob sieves after crushed and makes granular size be no more than 1mm; Be 2.0% H with the corn cob volume fraction after pulverizing
2SO
4Solution is pressed corn cob: H
2SO
4The solid-to-liquid ratio g:mL of solution is 1:10 100 ℃ of hydrolysis 2h in hydrolyzer, gets hemicellulose hydrolysate;
⑵ detoxification decolouring is processed: add Ca (OH) in the hemicellulose hydrolysate that obtains in step ⑴
2Transfer pH to 9, get supernatant liquor after solid-liquid separation, the supernatant liquor volume fraction is 10% H
3PO
4Solution is transferred pH to 6, and solid-liquid separation gets supernatant liquor; Again to through Ca (OH)
2In supernatant liquor after detoxification treatment by solid-to-liquid ratio 1:50(g:mL) add gac, be incubated 0.5h under 50 ℃, obtain the hemicellulose hydrolysate after the detoxification decolouring is processed after filtration;
After testing, obtain after filtration that in hemicellulose hydrolysate after detoxification decolouring is processed, Xylose Content is 25.21g/L;
⑶ filamentous fungus is cultivated:
1. strain inclined plane is cultivated: adopting the PDA substratum is slant medium, and graceful actinomucor thalline is seeded on slant medium, cultivates 3d for 28 ℃ in constant incubator, takes out after growing a large amount of spores on the inclined-plane;
2. spore suspension preparation: with the physiological saline 10mL wash-out spore of the bacterium of going out, two-layer sterile gauze removes by filter mycelium, filtrate is fully vibrated obtains spore suspension, and with the blood counting chamber counting, making ultimate density with the spore in distilled water adjustment spore suspension is 10
7mL
– 1
3. the fermentation culture of graceful actinomucor TCCC450001: add corn steep liquor 4.0%(corn steep liquor in the hemicellulose hydrolysate after the detoxification decolouring is processed: hydrolyzed solution, g:mL), KH
2PO
40.1%(KH
2PO
4: hydrolyzed solution, g:mL) and MgSO
47H
2O 0.6%(MgSO
47H
2O: hydrolyzed solution, g:mL), make fermention medium;
Add the 3L fermention medium in the 5L fermentor tank, with massfraction be 30% NaOH solution to regulate initial pH be 6, (spore concentration is 10 to access 240mL spore suspension in the substratum
6mL
– 1), control 26 ℃ of culture temperature, mixing speed 50r/min, air flow 1vvm cultivates 48h, obtains fermented liquid;
⑷ mycelium deproteinated: with filtering fermentation liquor, obtain mycelium.Mycelium is ground after 36h in 60 ℃ of bakings, with massfraction be 4% NaOH solution (mycelium: alkali lye=1:40, g:mL) 110 ℃ of processing 1h, solid-liquid separation obtains the alkali insolubles, is washed till filtrate neutrality with distilled water;
⑸ chitosan extracts: the alkali insolubles volume fraction that will be washed till filtrate neutrality is 4% acetum (mycelium: acid solution=1:20, g:mL) process 4h in 50 ℃, solid-liquid separation gets supernatant liquor, slowly adding massfraction in the supernatant liquor is 30% NaOH solution, and the limit edged stirs, adjust pH to 10, a large amount of white flockss appear, collecting precipitation thing and be washed with distilled water to filtrate for neutral after standing 10min, 40 ℃ of oven dry are the chitosan crude product;
⑹ use 95% ethanol with the chitosan crude product again, and (chitosan: 95% ethanol=1:20, g:mL) washing is 3 times, and (chitosan: acetone=1:20, g:mL) washing is 2 times, and 40 ℃ of oven dry can obtain the chitosan sterling to use at last acetone.
After testing, the productive rate of chitosan is 2.19g/L.
A kind of method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan, step is as follows:
⑴ the hydrolysis of hemicellulose raw material: corn cob sieves after crushed and makes granular size be no more than 1mm; Be 2.0% H with the corn cob volume fraction after pulverizing
2SO
4Solution is pressed corn cob: H
2SO
4The solid-to-liquid ratio g:mL of solution is 1:10 120 ℃ of hydrolysis 3h in hydrolyzer, gets hemicellulose hydrolysate;
⑵ detoxification decolouring is processed: add Ca (OH) in the hemicellulose hydrolysate that obtains in step ⑴
2Transfer pH to 11, get supernatant liquor after solid-liquid separation, the supernatant liquor volume fraction is 10% H
2SO
4Solution is transferred pH to 8, and solid-liquid separation gets supernatant liquor; Again to through Ca (OH)
2In supernatant liquor after detoxification treatment by solid-to-liquid ratio 1:25(g:mL) add gac, be incubated 2h under 50 ℃, obtain the hemicellulose hydrolysate after the detoxification decolouring is processed after filtration;
After testing, obtain after filtration that in hemicellulose hydrolysate after detoxification decolouring is processed, Xylose Content is 30.91g/L;
⑶ filamentous fungus is cultivated:
1. strain inclined plane is cultivated: adopting the PDA substratum is slant medium, and graceful actinomucor thalline is seeded on slant medium, cultivates 7d for 28 ℃ in constant incubator, takes out after growing a large amount of spores on the inclined-plane;
2. spore suspension preparation: with the physiological saline 10mL wash-out spore of the bacterium of going out, two-layer sterile gauze removes by filter mycelium, filtrate is fully vibrated obtains spore suspension, and with the blood counting chamber counting, making ultimate density with the spore in distilled water adjustment spore suspension is 10
8mL
– 1
3. the fermentation culture of graceful actinomucor TCCC450001: add corn steep liquor 5.0%(corn steep liquor in the hemicellulose hydrolysate after the detoxification decolouring is processed: hydrolyzed solution, g:mL), KH
2PO
40.6%(KH
2PO
4: hydrolyzed solution, g:mL) and MgSO
47H
2O 0.1%(MgSO
47H
2O: hydrolyzed solution, g:mL), make fermention medium;
Add the 3L fermention medium in the 5L fermentor tank, with massfraction be 30% NaOH solution to regulate initial pH be 7, (spore concentration is 10 to access 120mL spore suspension in the substratum
6mL
– 1), control 32 ℃ of culture temperature, mixing speed 150r/min, air flow 2vvm cultivates 64h, obtains fermented liquid;
⑷ mycelium deproteinated: with filtering fermentation liquor, obtain mycelium, mycelium is ground after 80 ℃ of baking 48h, it is 6% NaOH solution (mycelium: alkali lye=1:60 with massfraction, g:mL) process 4h for 120 ℃, solid-liquid separation obtains the alkali insolubles, is washed till filtrate neutrality with distilled water;
⑸ chitosan extracts: the alkali insolubles volume fraction that will be washed till filtrate neutrality is 6% acetum (mycelium: acid solution=1:60, g:mL) process 6h in 70 ℃, solid-liquid separation gets supernatant liquor, slowly adding massfraction in the supernatant liquor is 30% NaOH solution, and the limit edged stirs, adjust pH to 12, a large amount of white flockss appear, collecting precipitation thing and be washed with distilled water to filtrate for neutral after standing 15min, 60 ℃ of oven dry are the chitosan crude product;
⑹ use 95% ethanol with the chitosan crude product again, and (chitosan: 95% ethanol=1:20, g:mL) washing is 3 times, and (chitosan: acetone=1:20, g:mL) washing is 2 times, and 60 ℃ of oven dry can obtain the chitosan sterling to use at last acetone.
After testing, the productive rate of chitosan is 2.68g/L.
Claims (10)
1. method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan, it is characterized in that: step is as follows:
⑴ hemicellulose raw material hydrolysis: lignocellulosic material is pulverized, pressed lignocellulosic material: H
2SO
4The solid-to-liquid ratio g:mL of solution is that to add volume fraction be 0.5～2.0% H to the ratio of 1:8 ~ 11
2SO
4Solution at 100～120 ℃ of hydrolysis 1～3h, gets hemicellulose hydrolysate;
⑵ detoxification decolouring is processed: add Ca (OH) in hemicellulose hydrolysate
2Transfer pH to 9～11, the supernatant liquor after solid-liquid separation is transferred pH to 6～8, is then that 1:20～50 add gac by solid-to-liquid ratio g:mL, is incubated 0.5～2.0h under 50 ℃ and carries out the detoxification decolouring and process;
⑶ the cultivation of filamentous fungus: add corn steep liquor, KH in the hemicellulose hydrolysate after processing to the detoxification decolouring
2PO
4And MgSO
47H
2O makes fermention medium, after access filamentous fungus spore suspension, gets fermented liquid through fermentation culture in the fermention medium;
Wherein, hemicellulose hydrolysate: corn steep liquor: KH
2PO
4: MgSO
47H
2The ratio mL:g:g:g of O is 100:4.0～7.0:0.1～0.6:0.1～0.6;
⑷ mycelium deproteinated: with filtering fermentation liquor, obtain mycelium, to grind after the mycelium oven dry, according to mycelium: the ratio g:mL of alkali lye is that to add massfraction be 2～6% alkali lye to the ratio of 1:20～60, process 2～4h for 90 ~ 110 ℃, solid-liquid separation obtains the alkali insolubles, is washed till filtrate neutrality;
⑸ chitosan extracts: in the alkali insolubles that is washed till filtrate neutrality according to mycelium: the ratio g:mL of acid solution is that to add volume fraction be 2～6% weakly acid soln in 1:20～60, process 2～6h in 50～70 ℃, solid-liquid separation, get supernatant liquor, adjust pH is 9～12, standing 5～15min, the collecting precipitation thing also washs to filtrate as neutral, solid-liquid separation, 40～60 ℃ of oven dry namely get chitosan.
2. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1, it is characterized in that: in described step ⑴, lignocellulosic material sieves after crushed and makes granular size be no more than 1mm.
3. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1 is characterized in that: in described step ⑴, lignocellulosic material is one or more the mixture in maize straw, corn cob, straw, wheat straw.
4. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1 is characterized in that: transferring the solution that o'clock uses pH to 6～8 in described step ⑵ is 10% H as volume fraction
2SO
4Or H
3PO
4Solution.
5. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1 is characterized in that: in described step ⑶, access filamentous fungus spore suspension is for being 10 with spore suspension concentration
6～10
8mL
– 1The filamentous fungus spore suspension access by inoculum size 4～8%.
6. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1, it is characterized in that: in described step ⑶, fermentation culture conditions is: initial pH 6～7,26～34 ℃ of culture temperature, mixing speed 50～200r/min, air flow 0.5～2vvm, inoculum size 4～8%, incubation time 48～64h.
7. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1, it is characterized in that: in described step ⑶, filamentous fungus is graceful actinomucor TCCC450001(Actinomucor elegans).
8. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1, it is characterized in that: in described step ⑷, alkali lye is NaOH solution.
9. the method for utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan according to claim 1, is characterized in that: use NaOH solution when described step ⑸ transfers pH; Described weak acid is acetic acid.
10. the described method of utilizing wood sugar in hemicellulose hydrolysate to prepare chitosan of according to claim 1 to 9 any one, it is characterized in that: described chitosan also passes through following processing: with 95% washing with alcohol 2 ~ 4 times, use again washing with acetone 1 ~ 3 time, 40～60 ℃ of oven dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027849.3A CN103103233B (en) | 2013-01-25 | 2013-01-25 | Method for preparing chitosan by xylose in hemicellulose hydrolysate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027849.3A CN103103233B (en) | 2013-01-25 | 2013-01-25 | Method for preparing chitosan by xylose in hemicellulose hydrolysate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103103233A true CN103103233A (en) | 2013-05-15 |
| CN103103233B CN103103233B (en) | 2015-02-18 |
Family
ID=48311426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310027849.3A Expired - Fee Related CN103103233B (en) | 2013-01-25 | 2013-01-25 | Method for preparing chitosan by xylose in hemicellulose hydrolysate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103103233B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0542249A2 (en) * | 1991-11-13 | 1993-05-19 | Shin-Etsu Chemical Co., Ltd. | Method for preparing chitosan |
| CN101838346A (en) * | 2010-05-20 | 2010-09-22 | 广西工学院 | Method for preparing chitosan |
| CN102321192A (en) * | 2011-07-20 | 2012-01-18 | 深圳大学 | New preparation method of housefly larva chitosan |
-
2013
- 2013-01-25 CN CN201310027849.3A patent/CN103103233B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0542249A2 (en) * | 1991-11-13 | 1993-05-19 | Shin-Etsu Chemical Co., Ltd. | Method for preparing chitosan |
| CN101838346A (en) * | 2010-05-20 | 2010-09-22 | 广西工学院 | Method for preparing chitosan |
| CN102321192A (en) * | 2011-07-20 | 2012-01-18 | 深圳大学 | New preparation method of housefly larva chitosan |
Non-Patent Citations (3)
| Title |
|---|
| 任素霞等: "玉米芯中木聚糖水解制备D－木糖的试验研究", 《河南科学》 * |
| 刘珍利等: "培养基组成对雅致放射毛霉发酵生产壳聚糖的影响", 《天津科技大学学报》 * |
| 吕晓霞等: "玉米芯酶解液真菌发酵制备壳聚糖的研究", 《辽宁化工》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103103233B (en) | 2015-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101870739B (en) | Paenibacillus polymyxa extracellular polysaccharide and application thereof | |
| CN101589151A (en) | Method for production of ethanol or lactic acid | |
| CN101423855B (en) | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover | |
| CN103993042A (en) | Method for combined production of bioethanol and pullulan from lignocellulose substances | |
| CN103627644B (en) | A kind of yeast saccharomyces cerevisiae dissociant and application thereof | |
| CN103773824A (en) | Bacterial cellulose fermentation medium and method for producing bacterial cellulose by utilizing medium | |
| CN106434770B (en) | Method for producing ethanol by fermenting penicillium chrysogenum residues | |
| CN108486017A (en) | A kind of garden waste degradation bacterial agent | |
| CN105420127A (en) | High-yielding strain of high molecular weight pullulan and method for producing high molecular weight pullulan by utilizing high-yielding strain | |
| CN104109636B (en) | Aspergillus versicolor SD-3 and in the application prepared in chitin deacetylase | |
| CN103789349A (en) | Method for increasing microbe conversion rate of bagasse by utilizing non-ionic surface active agent | |
| CN103421851B (en) | A kind of method preparing sugar and ethanol with sweet potato waste | |
| CN103060418A (en) | Method of constructing mixed bacteria system for fermenting straw stalks to produce ethanol | |
| CN104561127A (en) | Comprehensive utilization method of agricultural straw | |
| CN102234670B (en) | Method for producing bacterial cellulose through solid state fermentation by using inert adsorption carrier | |
| CN102382771B (en) | Strain produced by beta-glucosidase and method for preparing Genipin therefrom | |
| CN115896179A (en) | Method for producing high-added-value product by utilizing brewing wastewater from monascus makinoi extremely | |
| CN102816751B (en) | High-activity chitosanase and preparation method thereof | |
| CN102492634B (en) | High-temperature resistant yeast and application thereof | |
| CN102127532B (en) | Method for using transgenic Coprinus cinereus to efficiently express recombinant enzyme | |
| CN102643754B (en) | Aspergillus oryzae and application thereof in aspect of improving yield of alcohol | |
| CN104726515A (en) | Method for extracting bacterial exopolysaccharide rich in fucose | |
| CN109486693A (en) | A kind of S. cervisiae and its purposes in alcohol fermentation | |
| CN103865803B (en) | Beta-glucosidase Producing Strain and the application prepared in genipin and trans-resveratrol in conversion thereof | |
| CN103103233B (en) | Method for preparing chitosan by xylose in hemicellulose hydrolysate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150218 Termination date: 20180125 |