CN103103142B - Staphylococcus cohnii and applications thereof - Google Patents
Staphylococcus cohnii and applications thereof Download PDFInfo
- Publication number
- CN103103142B CN103103142B CN201110353742.9A CN201110353742A CN103103142B CN 103103142 B CN103103142 B CN 103103142B CN 201110353742 A CN201110353742 A CN 201110353742A CN 103103142 B CN103103142 B CN 103103142B
- Authority
- CN
- China
- Prior art keywords
- staphylococcus
- fsdn
- bacterial strain
- strain
- denitrogenation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a strain of Staphylococcus cohnii, and applications thereof. The strain is Staphylococcus cohnii FSDN-C, and is preserved in the China General Microbiological Culture Collection Center on July 14, 2011, wherein a preservation number is CGMCCNO.5062. According to the present invention, the strain can adopt nitrite nitrogen as a substrate to complete short time denitrification denitrogenation, wherein organic pollutants can be removed during denitrogenation; when the Staphylococcus cohnii FSDN-C is adopted to treat ammonia-containing wastewater, characteristics of simple process, and high concentration organic carbon source tolerance are provided; after the strain is poured, the system is rapidly started; and the strain has broad application prospects in various wastewater denitrogenation treatment processes.
Description
Technical field
The invention belongs to environmental microorganism field, be specifically related to a kind of efficient nitrite type (short-cut denitrification) denitrification bacterial strain.Denitrogenation bacterial strain of the present invention can be take nitrite nitrogen as nitrogenous source carries out denitrification, is specially adapted to the denitrogenation in ammonia-containing water short-cut nitrification and denitrification process.
Background technology
Traditional biological denitrification process mainly comprises two processes of nitrification and denitrification, and nitrated is the prerequisite of denitrification process, and denitrification process could really remove the nitrogenous compound in waste water.Because nitrifier has strong aerobic, nitrifying process is aerobic; And denitrifying bacteria is generally facultative bacteria, under aerobic conditions, using oxygen as electron acceptor(EA), carry out aerobic repiration, while only having anaerobic state, just using nitrate or nitrite as electron acceptor(EA), obtain the energy of synthetic cell body.It is generally acknowledged under aerobic condition, nitric acid or nitrite reductase can not be expressed or oxygen molecule can produce and suppress these enzymes.When being greater than respectively 4.05mg/L, 2.15 mg/L, 0.25mg/L when dissolved oxygen
pseudomonas nauticanitrate reductase, nitrite reductase, reduction of nitrogen oxide enzyme complete deactivation.
Traditional denitrification process is that nitrification and denitrification is arranged in two structures and carries out classification denitrogenation mostly, exists sewage treatment process longer, and initial cost is high, need to supplement to nitrification tank the deficiencies such as basicity.So some denitrification microorganisms novel, better effects if, as allotrophic nitrobacteria, aerobic denitrifying bacteria, anaerobic ammonium oxidizing bacteria etc. are found in succession.
Isolated so far more than 60 kind of denitrifying bacteria, be mainly distributed in Pseudomonas (
pseudomonas), Alkaligenes (
alcaligeneas), general foster sulphur Coccus (
thiosphera pantotroph), Zoogloea (
zoogloeas)and bacillus (
bacillus)deng, great majority are facultative anaerobe.Lukow isolates and belongs to from the nitrated workshop section of percolate
zoogloeasone strain bacterium TL1(Aerobic denitrification by a newly isolated heterotrophic bacterium strain TL1[J]. Biotechnology Letters, 1997
11 (19): 1 157～1 159), this bacterium can utilize oxygen and degrade nitrate simultaneously.Naokia sieves to obtain two strain denitrifying bacterias, is respectively
pseudomonas stutzeritR2 and
pseudomonas sp.K50(Aerobic Denitrifying Bacteria that Produce low Levels of Nitrous Oxide [J] .Applied and Environmental Microbiology, 2003,69 (6): 3152-3157).Zhang Guangya (Huaqiao University's journal, 2004,25 (1)) isolates a strain from soil take the aerobic denitrifying bacteria HN that SODIUMNITRATE is nitrogenous source, through being accredited as
rhodococcus sp., Wang Lin etc. (environmental science, 2009, (30) 1) take nitrate nitrogen as electron acceptor(EA), the Trisodium Citrate of take as carbon source, from fluvial deposit, be separated to a strain Bacillus foecalis alkaligenes (
acaligenes faecalis).Wang Xiaoyu etc. (ACTA Scientiae Circumstantiae, 2009, (29) 1) from active sludge, be separated to false unit cell Alcaligenes (
pseudomonas pseudoalcaligenes) and Rhodopseudomonas Mendoza bacterium (
pseudomonas mendocina).Above the research of denitrifying microorganism is mainly concentrated on and take the denitrifying bacteria that nitrate nitrogen is electron acceptor(EA).Zhang little Ling etc. (microbiology circular, 2008,35 (10)) are separated to a strain aerobic denitrifying bacteria H2 from sediment of pond, through preliminary evaluation, belong to bacillus, and this bacterial strain is 0.885mg/ (L to the highest degradation rate of nitrite
.d); Li Bing etc. (aquatic ecological magazine, 2009,2 (3)) screen genus bacillus D5 from soil, and the most degradation speed of this bacterial strain is 2.22mg/ (L
.h).Healthy etc. (Research of Environmental Sciences, 2010,23 (1)) from prawn culturing pond, screen a bacillus coagulans (
bacillus coagulans) YX-6, this bacterial strain can be down to 0 by nitrite nitrogen by 10mg/L in 14h.The degrading nitrite nitrogen bacterial strain being more than separated to all belong to bacillus (
bacillus), these bacterial strains can be take nitrite nitrogen and be carried out denitrification denitrogenation as electron acceptor(EA), and degradation rate can reach 10mg/ (Lh), and the nitrite nitrogen concentration of the waste water of processing has also reached 100mg/L.
Staphylococcus is as a kind of environmental microorganism, and separation screening is to the strain bacterium that has degrading methamidophos, ethylbenzene, 2-picoline and azo dyes and have decoloring ability.Deng Xianyu etc. (ocean and marsh, 2009,40 (5)) from the acephatemet waste water in Xiang Tan Nan Tian chemical plant and mud, be separated to a strain be Staphylococcus pasteuri (
staphylococcus pasteuri), bacterial strain HN003 is 40000-45000mg/L to acephatemet maximum tolerated concentration.CN200610046643.5 discloses a staphylococcus xylosus
staphylococcus xylousy1, this bacterial strain has efficient degradation performance to ethylbenzene; CN200610046645.4 discloses and has opened a staphylococcus xylosus
staphylococcus xylousb1, this bacterial strain has higher degradation property to 2-picoline; CN201010103745.2 disclose a staphylococcus pasteuri (
staphylococcus pasteuri), this bacterial strain has the Azo dye decol ability of wide spectrum, and can adapt to extreme environment, under strong alkaline condition, still can decolour by azo dyes.Dalian University of Technology's environment and Life Sciences environmental engineering institute screen and obtain strains A ZR and belong to the Staphylococcus cohnis (Staphylococcus cohnii) in Staphylococcus (Staphylococcus) from dye wastewater treatment factory mud, and this bacterial strain has the ability that azo dyes decolours.There is not yet staphylococcus for the treatment of the report of trade effluent Nitrite Nitrogen.
Summary of the invention
The object of the present invention is to provide a kind of have spontaneous growth advantage, directly using nitrite as the Staphylococcus cohnis of electron acceptor(EA), Staphylococcus cohnis activity of nitrous acid reductase provided by the invention is high, under aerobic and oxygen free condition, all can express.Finding that there is of new bacterial strain is beneficial to promotion short-cut nitrification and denitrification technique and the application of synchronous nitration and denitrification technique in actual sewage treating processes.
The invention provides a strain staphylococcus, Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C, be preserved in that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number is CGMCC NO.5062 on July 14th, 2011.
Staphylococcus cohnis provided by the invention (
staphylococcus cohnii) FSDN-C Main Morphology is characterized as: colony colour for white, bacterial strain is individual is shaft-like, without gemma.
Staphylococcus cohnis of the present invention (
staphylococcus cohnii) physiological and biochemical property of FSDN-C shows as: gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.
Staphylococcus cohnis FSDN-C of the present invention can be take nitrite nitrogen and be carried out denitrification denitrogenation as nitrogenous source, and the expression of nitrite reductase is not limited by dissolved oxygen conditions, under aerobic and anaerobic environment, all can carry out denitrification.
The 16SrRNA gene order of Staphylococcus cohnis FSDN-C provided by the invention is shown in sequence table.
Staphylococcus cohnis FSDN-C provided by the invention, is mainly used in short-cut denitrification denitrogenation.This strain growth speed is fast, cell yield is high, requires C/N than low, no matter is that fresh medium or the nutrient solution of very low temperature preservation all have higher denitrification activity.Under the condition that this bacterial strain is 9.0 at pH, still can grow.The condition of this bacterial strain short-cut denitrification denitrogenation is: 15 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is 0.2 ~ 1.5mg/L; Carbon-nitrogen mass ratio 3:1 ~ 10:1.
Staphylococcus cohnis FSDN-C of the present invention, can enduring high-concentration nitrite nitrogen, reach as high as 400mg/L when processing nitrogenous effluent, and degradation rate can be greater than 10mg/ (L
.h).
Staphylococcus cohnis FSDN-C of the present invention can process brine waste, and tolerance salts contg can reach 25g/L, in denitrogenation, can remove COD.
Staphylococcus cohnis of the present invention is when processing nitrogenous effluent, and technique is simple, can enduring high-concentration organic carbon source.After coming into operation, system starts fast, in all kinds of denitrogenation of waste water treating processess, there is boundless application prospect, be particularly suitable for, in short-cut nitrification and denitrification or synchronous nitration and denitrification process, in the situation that denitrification effect is undesirable, can be used as bioremediation agents and adding.Can also prepare denitrogenation microbial inoculum.
biomaterial preservation explanation
Staphylococcus cohnis provided by the invention (
staphylococcus cohnii) FSDN-C bacterial strain, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC); Address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Deposit number: CGMCC NO.5062; Preservation date: on July 14th, 2011.
Embodiment
Staphylococcus cohnis of the present invention (
staphylococcus cohnii) screen in Fushun No.3 Petroleum Factory of FSDN-C Shi Cong CNPC sludge sewage and obtain, the nitrite nitrogen of can take carries out denitrification denitrogenation as nitrogenous source, and its physiological and biochemical test the results are shown in Table 1.
The physiological and biochemical test result of table 1 Staphylococcus cohnis FSDN-C.
| Test subject | Result | Test subject | Result | Test subject | Result |
| Cellular form | Spherical | Gramstaining | Positive | Catalase | ＋ |
| Nitrate reduction | － | VP reaction | ＋ | 15%NaCl | ＋ |
| Contrast | － | A-D-lactose | － | Tagatose | － |
| A-cyclodextrin | － | Lactulose | － | D-trehalose | ＋ |
| B-dextrin | － | Maltose | ＋ | Turanose | － |
| Dextrin | ＋ | Trisaccharide maltose | ＋ | Xylitol | － |
| Glycogen | － | PEARLITOL 25C | ＋ | D-wood sugar | － |
| Inulin | － | D-MANNOSE | ＋ | Acetic acid | － |
| Mannosans | － | Melizitose | － | A-hydroxybutyric acid | － |
| Tween40 | ＋ | D-melibiose | － | B-hydroxybutyric acid | － |
| Tween80 | － | A-methylgalactoside | － | G-hydroxybutyric acid | － |
| N-Acetyl-D-glucosamine | － | B-methylgalactoside | － | Amygdalic acid | － |
| ManNAc | － | 3-methyl D-glucose | － | A-keto-glutaric acid | － |
| Amygdaloside | － | A-methyl glucoside | － | A-ketone valeric acid | ＋ |
| L-arabinose | － | B-methyl glucoside | － | Lactic amide | － |
| D-R alcohol | ＋ | A-methyl mannoside | － | Methyl lactate | － |
| Arbutin | － | Palatinose | － | Pfansteihl | ＋ |
| D-cellobiose | － | D-Psicose | ＋ | D-malic acid | － |
| D-Fructose | ＋ | D-raffinose | － | L MALIC ACID | － |
| Fucose | － | L-rhamnosyl | － | Pyruvic Acid Methyl ester | ＋ |
| D-semi-lactosi | ＋ | D-ribose | ＋ | Succinic acid one methyl esters | － |
| Galacturonic acid | － | Saligenin | － | Propionic acid | － |
| Gentiobiose | － | Sedoheptulosan | － | Pyruvic acid | － |
| D-glyconic acid | ＋ | D-glucitol | － | Succinamic acid | b |
| A-D-glucose | ＋ | Stachyose | － | Succinic acid | － |
| M-inositol | － | Sucrose | ＋ | N-acetylglutamat | － |
| Oxydase | － | Pyrrolidonecarboxylic acid | － | Uridine | ＋ |
| PH4.5 growth | ＋ | Serine | ＋ | Acyl thuja acid | － |
| L alanimamides | － | Putrescine | － | Thymidylic acid | － |
| D-alanine | ＋ | 2,3-butanediol | － | Uridylic acid | － |
| ALANINE | ＋ | Glycerine | ＋ | D-Fructose sugar-6-phosphoric acid | － |
| L-alanyl-glycine | ＋ | Adenosine | － | D-glucose-1-phosphate | － |
| Altheine | － | 2-Desoxyadenosine | － | D-Robison ester | － |
| Pidolidone | ＋ | Inosine | ＋ | D, L-glycerophosphate | － |
| Glycyl L-glutamic acid | － | Thymidine | － |
Through " China Committee for Culture Collection of Microorganisms common micro-organisms " center " identifies, this bacterial strain belong to Staphylococcus cohnis (
staphylococcus cohnii) called after FSDN-C.Fig. 1 is the growth conditions of this Staphylococcus cohnis on agar plate, and this bacterium colony is white, emulsus, colour purity.
enrichment culture and the separation and purification of embodiment 1 bacterium
The enrichment culture of denitrifying bacterium:
Get the active sludge in the nitrification tank of sewage farm, utilize the substratum that contains nitrite under low DO condition, to carry out enrichment culture certain hour, obtain denitrifying activated sludge.In enrichment process, supplement fresh medium in good time.
Enrichment culture liquid formula: NaNO
2, methyl alcohol, also contains and comprises Fe
2+, Mg
2+, K
+, Ca
2+four kinds of metallic cations.Culture condition is: 30 ℃ of temperature; PH is 8.2, DO(dissolved oxygen) be 0.2-1.0 mgL
– 1.
Mole allocation ratio of four kinds of metallic cations is 1:(4-8): (5-15): (1-5).In culturing process, add the growth stimulant that metallic cation total concn is 0.15mol/L every day, and additional amount is 0.1% of the interior volume of material of reactor.
The screening of denitrifying bacterium:
Draw the above-mentioned enrichment culture liquid of 1ml, resuspended and as far as possible particle is broken up with distilled water, get after bacteria suspension dilutes different multiples and be coated with the beef extract-peptone flat board that has permeated denitrification nutrient solution, the screening of denitrifying bacterium is carried out in 30 ℃ of cultivations, the upgrowth situation of routine observation thalline in culturing process, repeats aforesaid operations until obtain single bacterium colony.Individual plant purifying is carried out in single bacterium colony line on picking flat board, transfers twice, obtains the bacterial strain FSDN-C of purifying.
Separation and Culture based formulas: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, flat board adds 2% agar.
embodiment 2 bacterial strain denitrification ability of the present invention is investigated
First from flat board picking list bacterium colony FSDN-C be inoculated in beef-protein medium 30 ℃, 120r/min shaking table and cultivate 48h after cell concentration maximum, finish to cultivate.After bacteria suspension is centrifugal, proceed to and in Sodium Nitrite-methyl alcohol nutrient solution, under similarity condition, take batch formula to change water and two kinds of modes of batch feed supplement are carried out denitrification ability investigation.Table 1 is that batch formula is changed under regimen condition removal effect when bacterial strain of the present invention is to nitrite nitrogen and COD.Data from table, water inlet total nitrogen concentration is brought up in 469mg/L process by 200mg/L, and nitrogen removal rate is greater than 88.63% all the time, and inlet COD concentration is brought up in 4149mg/L process by 2728mg/L, and COD clearance is greater than 80.14%.Visible this bacterial strain can also be removed high concentration COD when processing total nitrogen.
Table 1 FSDN-C bacterial strain denitrification ability domestication result (mg/L).
| Water inlet NO 2 - | Total N of intaking | Influent COD | Reaction times, h | Total N clearance, % | COD clearance, % |
| 178.3 | 211 | 2728 | 48 | 88.63 | 93.22 |
| 257.5 | 293 | 2850 | 24 | 93.52 | 86.77 |
| 294.9 | 372 | 3017 | 20 | 93.82 | 81.17 |
| 312.7 | 469 | 4149 | 18 | 91.68 | 80.14 |
embodiment 3 bacterial strain of the present invention is processed certain oil refinery effluent
Get bacteria suspension that a certain amount of embodiment 2 obtains according to 15%(volume) inoculum size inoculate, at 28 ℃ of temperature, pH, be 8.3, DO(dissolved oxygen) be 1.2mgL
– 1under condition, the water outlet of certain refinery water that processing contains alkaline residue after Air Exposure, wherein inorganic salt content is 25g/L, NO
2 --N average out to 120mg/L, COD average out to 800 mg/L.Water outlet NO after treatment
2 --N and COD mean concns are respectively 6.4mg/L and 61mg/L, NO
2 --N and COD average removal rate are respectively 94.7% and 92.4%.
the application of embodiment 4 bacterial strain of the present invention in containing wastewater from catalyst denitrification process
Get bacteria suspension that a certain amount of embodiment 2 obtains according to 15%(volume) inoculum size inoculate, at 30 ℃ of temperature, pH, be 7.8, DO(dissolved oxygen) be 0.3mgL
– 1under condition, the ammonia-containing water short distance nitration water outlet producing in Catalyst Production process is carried out to denitrification processing, in this water, COD is 800 mg/L ~ 1200mg/L, NO
2 --N is 500 mg/L ~ 780mg/L, NO
3 --N is 10mg/L.Determination of Total Nitrogen in Waste Water clearance reaches more than 92% after treatment.
SEQUENCE LISTING
<110> Sinopec Group
China Petroleum and Chemical Corporation Fushun Petrochemical Research Institute
<120> mono-strain staphylococcus and application thereof
<130> newly applies for
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1421
<212> DNA
<213> Staphylococcus cohnii
<400> 1
tctgcagtcg agcgaacaga taaggagctt gctcctttga cgttagcggc ggacgggtga 60
gtaacacgtg ggtaacctac ctataagact ggaataactc cgggaaaccg gggctaatgc 120
cggataacat ttagaaccgc atggttctaa agtgaaagat ggttttgcta tcacttatag 180
atggacccgc gccgtattag ctagttggta aggtaacggc ttaccaaggc aacgatacgt 240
agccgacctg agagggtgat cggccacact ggaactgaga cacggtccag actcctacgg 300
gaggcagcag tagggaatct tccgcaatgg gcgaaagcct gacggagcaa cgccgcgtga 360
gtgatgaagg tcttcggatc gtaaaactct gttattaggg aagaacaaat gtgtaagtaa 420
ctatgcacgt cttgacggta cctaatcaga aagccacggc taactacgtg ccagcagccg 480
cggtaatacg taggtggcaa gcgttatccg gaattattgg gcgtaaagcg cgcgtaggcg 540
gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt ggaaactggg 600
aaacttgagt gcagaagagg aaagtggaat tccatgtgta gcggtgaaat gcgcagagat 660
atggaggaac accagtggcg aaggcgactt tctggtctgt aactgacgct gatgtgcgaa 720
agcgtgggga tcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780
aagtgttagg gggtttccgc cccttagtgc tgcagctaac gcattaagca ctccgcctgg 840
ggagtacgac cgcaaggttg aaactcaaag gaattgacgg ggacccgcac aagcggtgga 900
gcatgtggtt taattcgaag caacgcgaag aaccttacca aatcttgaca tcctttgaca 960
actctagaga tagagccttc cccttcgggg gacaaagtga caggtggtgc atggttgtcg 1020
tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttaaacttag 1080
ttgccagcat ttagttgggc actctaagtt gactgccggt gacaaaccgg aggaaggtgg 1140
ggatgacgtc aaatcatcat gccccttatg atttgggcta cacacgtgct acaatggaca 1200
atacaaaggg cagctaaacc gcgaggtcat gcaaatccca taaagttgtt ctcagttcgg 1260
attgtagtct gcaactcgac tacatgaagc tggaatcgct agtaatcgta gatcagcatg 1320
ctacggtgaa tacgttcccg ggtcttgtac acaccgcccg tcacaccacg agagtttgta 1380
acacccgaag ccggtggagt aaccatttat ggagctagcc t 1421
Claims (8)
1. a strain Staphylococcus cohnis (Staphylococcus cohnii) FSDN-C, the deposit number at Qi China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC NO.5062, it is characterized in that: Staphylococcus cohnis FSDN-C is when processing nitrogenous effluent, can enduring high-concentration nitrite nitrogen, maximum concentration can reach 400mg/L, and degradation rate is greater than 10mg/ (Lh); And Staphylococcus cohnis FSDN-C can process brine waste, tolerance salts contg can reach 25g/L, in denitrogenation, can remove COD.
2. bacterial strain according to claim 1, is characterized in that the morphological specificity of Staphylococcus cohnis FSDN-C is: colony colour is white, and bacterial strain individuality is spherical, without gemma.
3. bacterial strain according to claim 1, is characterized in that the physiological and biochemical property of Staphylococcus cohnis FSDN-C is: gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.
4. according to the bacterial strain described in claim 1 or 3, it is characterized in that the expression of Staphylococcus cohnis FSDN-C nitrite reductase is not limited by dissolved oxygen conditions, under aerobic and anaerobic environment, all can carry out denitrification.
5. according to the bacterial strain described in claim 1 or 3, it is characterized in that can growing under condition that this bacterial strain is 9.0 at pH.
6. described in the arbitrary claim of claim 1～5, Staphylococcus cohnis FSDN-C is applied to short-cut denitrification denitrogenation.
7. described in the arbitrary claim of claim 1～5, Staphylococcus cohnis FSDN-C is applied to brine waste processing.
8. according to the application described in claim 6 or 7, it is characterized in that staphylococcus FSDN-C is 15 ℃～35 ℃ of temperature for the operational condition of wastewater treatment; PH6.5～10.0; Dissolved oxygen is 0.2～1.5mg/L; Carbon-nitrogen mass ratio 3:1～10:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110353742.9A CN103103142B (en) | 2011-11-10 | 2011-11-10 | Staphylococcus cohnii and applications thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110353742.9A CN103103142B (en) | 2011-11-10 | 2011-11-10 | Staphylococcus cohnii and applications thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103103142A CN103103142A (en) | 2013-05-15 |
| CN103103142B true CN103103142B (en) | 2014-09-17 |
Family
ID=48311344
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110353742.9A Active CN103103142B (en) | 2011-11-10 | 2011-11-10 | Staphylococcus cohnii and applications thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103103142B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388336B (en) * | 2014-10-27 | 2017-05-10 | 浙江工业大学 | Staphylococcus genera LWX with capability of acetic acid degradation and application thereof |
| CN104388334B (en) * | 2014-10-27 | 2017-06-27 | 浙江工业大学 | A kind of preparation method and applications of liquid composite bacteria agent |
| CN105112335B (en) * | 2015-09-06 | 2018-09-25 | 中国农业科学院烟草研究所 | The staphylococcus CGMCC No.10671 of enduring high-concentration glucose and its application |
| CN108118022B (en) | 2016-11-29 | 2020-09-11 | 中国石油化工股份有限公司 | Microbial culture promoter for completing denitrification process and application thereof |
| CN108048361B (en) * | 2017-12-28 | 2021-01-15 | 中国农业大学 | Staphylococcus cohnii S154 and application thereof in biosynthesis of nano-selenium |
| KR102138862B1 (en) * | 2019-03-08 | 2020-07-30 | 씨제이제일제당 주식회사 | Allulose-producing Staphylococcus microorganism and method for producing allulose using the same |
| CN110511894B (en) * | 2019-09-04 | 2020-06-16 | 北京星皓互动科技有限公司 | Composite biological agent and preparation and application thereof |
| CN110551655A (en) * | 2019-09-05 | 2019-12-10 | 北京星皓互动科技有限公司 | composite biological agent, preparation method thereof and application thereof in printing and dyeing textile sewage treatment |
| CN111909860B (en) * | 2020-07-30 | 2022-06-21 | 克拉玛依市新奥达石油技术服务有限公司 | Bacterial strain for producing biological emulsifier and biological emulsifier produced by using bacterial strain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009013443A1 (en) * | 2007-07-23 | 2009-01-29 | Vaccine Research International Plc | Inactivated staphylococcal whole-cell vaccine |
| CN101892179A (en) * | 2010-04-23 | 2010-11-24 | 中国地质大学（武汉） | Facultative anaerobic benzene compounds-degrading bacterium |
-
2011
- 2011-11-10 CN CN201110353742.9A patent/CN103103142B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009013443A1 (en) * | 2007-07-23 | 2009-01-29 | Vaccine Research International Plc | Inactivated staphylococcal whole-cell vaccine |
| CN101892179A (en) * | 2010-04-23 | 2010-11-24 | 中国地质大学（武汉） | Facultative anaerobic benzene compounds-degrading bacterium |
Non-Patent Citations (2)
| Title |
|---|
| 河口水体中的菌群分析及其脱氮能力的研究;葛美丽等;《河北渔业》;20091231(第2期);1-4 * |
| 葛美丽等.河口水体中的菌群分析及其脱氮能力的研究.《河北渔业》.2009,(第2期),1-4. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103103142A (en) | 2013-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103103142B (en) | Staphylococcus cohnii and applications thereof | |
| CN101570738B (en) | Agrobacterium with heterotrophic nitrification-aerobic denitrification capability and application thereof in nitrogenous effluent treatment | |
| CN102465105B (en) | Nitrous acid-type denitrification bacteria strain and application thereof | |
| CN102465103B (en) | Aerobic denitrification methylobacterium phyllosphaerae and application thereof | |
| CN110217895B (en) | Compound microbial agent for water environment treatment and application thereof | |
| CN108117221B (en) | Treatment method of reverse osmosis concentrated water | |
| CN109456926B (en) | Microbial agent containing halophilic denitrifying bacteria YL5-2 and application thereof | |
| CN104911130A (en) | Halomonas sp. with denitrogenation capability and application thereof | |
| CN113444661B (en) | Sphingobacterium neoformans and application thereof in wastewater dephosphorization | |
| CN103103141B (en) | Kocuria palustris strain and applications thereof | |
| CN106987547A (en) | One plant of Acinetobacter bauamnnii and its application | |
| CN109401997A (en) | One plant of Stenotrophomonas and its application and microbial bacterial agent | |
| CN104862260A (en) | Arthrobacter with aerobic denitrification capability and application thereof | |
| CN110791444B (en) | Pseudomonas stutzeri, composite microbial inoculum prepared from pseudomonas stutzeri and application of composite microbial inoculum | |
| CN113403234B (en) | Marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group driven to develop by marine self-flocculating bacterium as well as construction method and application of marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group | |
| CN113249273B (en) | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment | |
| CN102465106B (en) | Bacterial strain used for short-cut denitrification for nitrogen removal and its application | |
| CN114395505A (en) | Low-temperature denitrifying bacterium and application thereof | |
| CN111925960B (en) | Halomonas with nitrification and denitrification functions and application thereof | |
| CN112266885A (en) | Heterotrophic nitrification aerobic denitrifying bacterium Y16 and application thereof | |
| CN111979138A (en) | Heterotrophic nitrification aerobic denitrifying bacterium Y15 and application thereof | |
| CN109055259B (en) | Pseudomonas XD-3, application thereof and microbial flocculant | |
| CN111286478A (en) | Grease degradation composite bacterial agent and preparation method and application thereof | |
| CN116083281A (en) | Composite microbial inoculum based on zoology and application of composite microbial inoculum in decoloring printing and dyeing wastewater | |
| CN101412974B (en) | Brevibacterium aureum AN3 and use thereof in degrading malachite green and other dyes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |