CN103088112B - F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes - Google Patents
F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes Download PDFInfo
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- CN103088112B CN103088112B CN201110336049.0A CN201110336049A CN103088112B CN 103088112 B CN103088112 B CN 103088112B CN 201110336049 A CN201110336049 A CN 201110336049A CN 103088112 B CN103088112 B CN 103088112B
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Abstract
Description
Numbering | Ingredient names | Quantity | Preservation condition |
1 | chro prfA | 20μl | -20℃ |
2 | Synthesis buffer | 10ml | Room temperature |
3 | ADP(1.6mol/l) | 1ml | -20℃ |
4 | 1×PBS | 25ml | Room temperature |
5 | Luciferase/luciferin | 100 units | -20℃ |
6 | Luciferase/luciferin reassembly buffer liquid | 12ml | -20℃ |
7 | Sterilized water | 5ml | Room temperature |
Sample (10ng/mL) | Fluorescent value |
Water | 284901 |
Singly increase listeria spp | 270174 |
Vibrio parahemolyticus | 300679 |
Salmonellas | 294943 |
Vibrio cholerae | 294649 |
Intestinal bacteria | 285223 |
Sample number | Fluorescent value | Sample number | Fluorescent value |
Water | 562689 | 16 | 305267 |
1 | 269526 | 17 | 303836 |
2 | 288540 | 18 | 335545 |
3 | 297241 | 19 | 322881 |
4 | 328428 | 20 | 312907 |
5 | 308957 | 21 | 313883 |
6 | 266580 | 22 | 311252 |
7 | 280415 | 23 | 309237 |
8 | 278235 | 24 | 296812 |
9 | 286678 | 25 | 307311 |
10 | 305228 | 26 | 317081 |
11 | 317380 | 27 | 317412 |
12 | 307364 | 28 | 300856 |
13 | 304734 | 29 | 300767 |
14 | 290645 | 30 | 286644 |
15 | 289172 | —— | —— |
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201110336049.0A CN103088112B (en) | 2011-10-31 | 2011-10-31 | F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201110336049.0A CN103088112B (en) | 2011-10-31 | 2011-10-31 | F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes |
Publications (2)
Publication Number | Publication Date |
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CN103088112A CN103088112A (en) | 2013-05-08 |
CN103088112B true CN103088112B (en) | 2015-06-03 |
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CN201110336049.0A Expired - Fee Related CN103088112B (en) | 2011-10-31 | 2011-10-31 | F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes |
Country Status (1)
Country | Link |
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CN (1) | CN103088112B (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101397586B (en) * | 2008-10-10 | 2011-02-02 | 广东省疾病预防控制中心 | Composite gene chip for food-borne pathogenic bacteria detection |
CN102135543A (en) * | 2010-01-27 | 2011-07-27 | 中国科学院生物物理研究所 | Real-time and sensitive novel method for detecting biomacromolecule and preparation method for kit |
CN102154473A (en) * | 2011-01-24 | 2011-08-17 | 杭州市农业科学研究院 | Gene chip and applications thereof in detection of aquatic pathogenic microorganism |
-
2011
- 2011-10-31 CN CN201110336049.0A patent/CN103088112B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101397586B (en) * | 2008-10-10 | 2011-02-02 | 广东省疾病预防控制中心 | Composite gene chip for food-borne pathogenic bacteria detection |
CN102135543A (en) * | 2010-01-27 | 2011-07-27 | 中国科学院生物物理研究所 | Real-time and sensitive novel method for detecting biomacromolecule and preparation method for kit |
CN102154473A (en) * | 2011-01-24 | 2011-08-17 | 杭州市农业科学研究院 | Gene chip and applications thereof in detection of aquatic pathogenic microorganism |
Non-Patent Citations (3)
Title |
---|
Evaluation of Paramagnetic Beads Coated with Recombinant Listeria Phage Endolysin-Derived Cell-Wall-Binding Domain Proteins for Separation of Listeria monocytogenes from Raw Milk in Combination with Culture-Based and Real-Time Polymerase Chain Reaction;Walcher Georg et al.;《FOODBORNE PATHOGENS AND DISEASE》;20100525;第7卷(第9期);第1020页右栏倒数第3段 * |
Taq Man-MGB探针Real-timePCR快速检测单增李斯特菌的研究;梅玲玲等;《中国卫生检验杂志》;20070210(第02期);全文 * |
实时荧光PCR定量检测食品中单增李斯特菌;熊国华等;《中国食品卫生杂志》;20070531(第03期);全文 * |
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CN103088112A (en) | 2013-05-08 |
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Inventor after: Zhang Jie Inventor after: Lu Xiaoyu Inventor after: Han Jing Inventor after: Liu Yan Inventor after: Gu Dezhou Inventor after: Chen Guangquan Inventor after: Yang Xiangying Inventor after: Zhang Yan Inventor after: Wan Xiaonan Inventor after: Zhang Lei Inventor after: Zhang Huiyuan Inventor after: Wang Qi Inventor after: Lu Lin Inventor after: Zhang Cuan Inventor before: Zhang Jie Inventor before: Chen Guangquan Inventor before: Zhang Huiyuan Inventor before: Wang Qi Inventor before: Lu Lin Inventor before: Zhang Cuan Inventor before: Lu Xiaoyu Inventor before: Han Jing Inventor before: Liu Yan Inventor before: Gu Dezhou |
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Free format text: CORRECT: INVENTOR; FROM: ZHANG JIE ZHANG HUIYUAN WANG QI LU LIN ZHANG XIN LU XIAOYU HAN JING LIU YAN GU DEZHOU CHEN GUANGQUAN TO: ZHANG JIE YANG XIANGYING ZHANG YAN WAN XIAONAN ZHANG LEI ZHANG HUIYUAN WANG QI LU LIN ZHANG XIN LU XIAOYU HAN JING LIU YAN GU DEZHOU CHEN GUANGQUAN |
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