CN103088155A - Molecular motor biosensor kit for detecting rotavirus - Google Patents
Molecular motor biosensor kit for detecting rotavirus Download PDFInfo
- Publication number
- CN103088155A CN103088155A CN2012104060731A CN201210406073A CN103088155A CN 103088155 A CN103088155 A CN 103088155A CN 2012104060731 A CN2012104060731 A CN 2012104060731A CN 201210406073 A CN201210406073 A CN 201210406073A CN 103088155 A CN103088155 A CN 103088155A
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- Prior art keywords
- rotavirus
- sample
- buffer
- detection
- molecular motor
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Numbering | The component title | | Preservation condition | |
1 | Chro VP7 | 20μl | -20 |
|
2 | Synthetic buffer | | Room temperature | |
3 | ADP(1.6mol/l) | 1ml | -20 |
|
4 | 1×PBS | | Room temperature | |
5 | Luciferase/luciferin | 100 units | -20℃ | |
6 | Luciferase/luciferin reassembly buffer liquid | 12ml | -20℃ | |
7 | Sterilized water | 5ml | Room temperature |
Sample (0.6ng/mL) | Fluorescent value |
Water | 351774 |
Rotavirus | 419008 |
Hepatitis A virus (HAV) | 356623 |
Norovirus | 350977 |
Sample number | Fluorescent value | Sample number | Fluorescent value |
Water | 389386 | 8 | 455971 |
1 | 419739 | 9 | 443609 |
2 | 445509 | 10 | 447321 |
3 | 457229 | 11 | 435403 |
4 | 400312 | 12 | 447404 |
5 | 391020 | 13 | 446144 |
6 | 434933 | 14 | 442853 |
7 | 451273 | 15 | 439719 |
Claims (3)
- The present invention claimed in have:1. specific dna probe and F 0F 1The mode that-atp synthase connects: at F 0F 1Connect successively epsilon subunit antibody, vitamin H, Streptavidin, biotin labeled rotavirus nucleic acid probe on the epsilon subunit of-atp synthase.
- 2. the reaction conditions of test kit detection system: room temperature incubation 10min.
- 3. the formula that synthesizes buffer, startup buffer, PBS.Synthetic buffer: glycerine 20%, magnesium chloride 5mM, Tricine50mM, dipotassium hydrogen phosphate 5mM; Start buffer:ADP (1.6mM): above-mentioned synthetic buffer=1: 3 (v/v); PBS:137mM sodium-chlor, 2.7mM Repone K, 10mM Sodium phosphate dibasic, 2mM potassium primary phosphate.
Priority Applications (1)
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CN2012104060731A CN103088155A (en) | 2012-10-23 | 2012-10-23 | Molecular motor biosensor kit for detecting rotavirus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012104060731A CN103088155A (en) | 2012-10-23 | 2012-10-23 | Molecular motor biosensor kit for detecting rotavirus |
Publications (1)
Publication Number | Publication Date |
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CN103088155A true CN103088155A (en) | 2013-05-08 |
Family
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Family Applications (1)
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CN2012104060731A Pending CN103088155A (en) | 2012-10-23 | 2012-10-23 | Molecular motor biosensor kit for detecting rotavirus |
Country Status (1)
Country | Link |
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CN (1) | CN103088155A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101429545A (en) * | 2008-10-17 | 2009-05-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Method for detecting Shigella by using suspension chip technology |
CN102135543A (en) * | 2010-01-27 | 2011-07-27 | 中国科学院生物物理研究所 | Real-time and sensitive novel method for detecting biomacromolecule and preparation method for kit |
-
2012
- 2012-10-23 CN CN2012104060731A patent/CN103088155A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101429545A (en) * | 2008-10-17 | 2009-05-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Method for detecting Shigella by using suspension chip technology |
CN102135543A (en) * | 2010-01-27 | 2011-07-27 | 中国科学院生物物理研究所 | Real-time and sensitive novel method for detecting biomacromolecule and preparation method for kit |
Non-Patent Citations (3)
Title |
---|
张捷等: "利用分子马达技术检测霍乱弧菌", 《食品与发酵工业》, vol. 38, no. 6, 30 June 2012 (2012-06-30) * |
王志宇等: "荧光定量RT-PCR在轮状病毒检测中的应用", 《山东大学学报(医学版)》, no. 03, 31 March 2006 (2006-03-31) * |
陈继军等: "TaqMan实时定量RT-PCR检测轮状病毒G4型", 《中国生物制品学杂志》, vol. 23, no. 11, 30 November 2010 (2010-11-30) * |
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Legal Events
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Jie Inventor after: Wang Qi Inventor after: Zhang Cuan Inventor after: Wang Peirong Inventor after: Chen Guangquan Inventor after: Le Jiachang Inventor after: Yang Lijun Inventor after: Yang Xiangying Inventor after: Wan Xiaonan Inventor after: Zhang Huiyuan Inventor after: Lu Xiaoyu Inventor after: Xu Meiling Inventor after: Gu Dezhou Inventor after: Liu Yan Inventor before: Zhang Jie Inventor before: Wang Peirong Inventor before: Chen Guangquan Inventor before: Le Jiachang Inventor before: Yang Lijun Inventor before: Zhang Huiyuan Inventor before: Lu Xiaoyu Inventor before: Xu Meiling Inventor before: Gu Dezhou Inventor before: Liu Yan Inventor before: Wang Qi Inventor before: Zhang Cuan |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG JIE YANG LIJUN ZHANG HUIYUAN LU XIAOYU XU MEILING GU DEZHOU LIU YAN WANG QI ZHANG XIN WANG PEIRONG CHEN GUANGQUAN LE JIACHANG TO: ZHANG JIE YANG LIJUN YANG XIANGYING WAN XIAONAN ZHANG HUIYUAN LU XIAOYU XU MEILING GU DEZHOU LIU YAN WANG QI ZHANG XIN WANG PEIRONG CHEN GUANGQUAN LE JIACHANG |
|
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130508 |