CN103088095A - Preparation method of sheep horn protein peptide - Google Patents
Preparation method of sheep horn protein peptide Download PDFInfo
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- CN103088095A CN103088095A CN 201110342968 CN201110342968A CN103088095A CN 103088095 A CN103088095 A CN 103088095A CN 201110342968 CN201110342968 CN 201110342968 CN 201110342968 A CN201110342968 A CN 201110342968A CN 103088095 A CN103088095 A CN 103088095A
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Abstract
The invention relates to a preparation method of sheep horn protein peptide. The method consists of: brushing a sheep horn surface, draining the sheep horn, and grinding it into fine powder, passing the powder through a 200-mesh sieve, soaking the sheep horn powder in twice as much n-hexane at room temperature for 24h, repeating the operation once, and removing the n-hexane so as to obtain defatted sheep horn powder; using a triple amount of 1.5% sodium hydroxide to extract sheep horn protein at 30DEG C for 2-5h, and conducting centrifugation to remove slag; adjusting the pH of the supernatant to 5.6 with hydrochloric acid to precipitate protein; carrying out centrifugation to remove the liquid, dissolving the residue in water, adjusting the pH to 6.5, adding 1398 protease according to a proportion of 5000U per gram of substrate, preserving the temperature at 35DEG C and conducting stirring, performing enzymolysis for 4-6h, implementing centrifugation, discarding the precipitate, and retaining the supernatant; heating the supernatant to 80-85DEG C, carrying out enzyme deactivation for 8-12min, conducting concentration, and performing spray drying, thus obtaining a sheep horn protein peptide product, in which the content of peptide with a molecular weight of 1000-3000 Daltons is up to over 65%.
Description
Technical field
A kind of preparation method of goat's horn protein peptide belongs to herding goods processing technique field.
Background technology
Livestock and poultry processing has been transitioned into present large-scale production by the workshop-based production in past; Xinjiang of China, Inner Mongol, Tibet, the every Year's consumption of Gansu beef and mutton are huge; and the cattle and sheep that a great deal of is arranged are slaughtered tooling pin toward each big city, the whole nation in the locality; its processing waste material goat's horn never obtains fine utilization; mostly topple over serious environment pollution as waste.Goat's horn contains more animal proteinum, contains 18 seed amino acids, is a kind of important animal proteinum source.The goat's horn protein peptide can be widely used in cosmetic industry and fodder industry, but does not also find more there is no relevant research for application and development with the bibliographical information of goat's horn processing protein peptide at present.Therefore with the goat's horn protein peptide, the environment protection that promotes the sheep manufacture field is arranged not only, and can turn waste into wealth, have important society and economic implications.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of goat's horn protein peptide, after the raw material pulverizing degreasing, alkali is carried the heavy preparation of acid albumen take the processing waste material goat's horn, through 1398 protease hydrolyzeds, and the enzyme that goes out, spraying drying obtains the goat's horn protein peptide.
Technical scheme of the present invention: a kind of preparation method of goat's horn protein peptide, get goat's horn outwash surface, drain, wear into fine powder, cross 200 mesh sieves, with above-mentioned goat's horn powder, measured the normal hexane soaking at room temperature 24 hours with 2 times, repeat once, slough normal hexane and obtain degreasing goat's horn powder.Extracted goat's horn albumen 2-5 hour, centrifugal removing slag with 3 times of amount 1.5% sodium hydroxide in 30 ℃.Supernatant liquor is regulated pH5-6 with hydrochloric acid and is made protein precipitation.The centrifugal liquid that goes, residue is water-soluble, transfers pH to 6.5, adds 1398 proteolytic enzyme in the ratio of 5000U/g substrate, 35 ℃ of insulated and stirred, enzymolysis 4～6 hours, centrifugal, discard precipitation, keep supernatant liquor.Above-mentioned supernatant liquor is warming up to 80～85 ℃, the enzyme 8～12 minutes of going out, concentrated, spraying drying gets goat's horn protein peptide product, and in its product, the daltonian peptide content of molecular weight 1000-3000 reaches more than 65%.
Beneficial effect of the present invention: the present invention adopts alkali extraction and acid precipitation take goat's horn as raw material, and the method for enzymolysis has prepared the goat's horn protein peptide easily.Technological basis goat's horn protein characteristic has been selected 1398 proteolytic enzyme cleverly, make the heavy pH of acid and enzymolysis pH comparatively approaching, reduced the content of salt in the product, thereby the steps such as desalination that need not conventional albumen preparation obtain can be used for the goat's horn protein peptide of fodder industry, facilitate approach for goat's horn develops to provide.
Table 1: the molecular weight distribution of product (%)
| 1000-2000 dalton | 2000-3000 dalton | 1000-3000 dalton |
| 25 | 43 | 68 |
Embodiment
Embodiment 1
Get 100 grams and cross the degreasing goat's horn powder of 200 mesh sieves, add 300 milliliter of 1.5% sodium hydroxide to extract goat's horn albumen 2 hours, centrifugal removing slag in 30 ℃.Supernatant liquor is regulated pH5.6 with hydrochloric acid and is made protein precipitation.The centrifugal liquid that goes, residue are dissolved in 300 ml waters, regulate pH to 6.5, add 500000U 1398 proteolytic enzyme, 35 ℃ of insulated and stirred, and enzymolysis 4 hours, centrifugal, discard precipitation, keep supernatant liquor.Above-mentioned supernatant liquor is warming up to 80～85 ℃, the enzyme 8 minutes of going out, concentrated, spraying drying gets goat's horn protein peptide product, and in its product, the daltonian peptide content of molecular weight 1000-3000 reaches 66.2%.
Embodiment 2
Get 100 grams and cross the degreasing goat's horn powder of 200 mesh sieves, add 300 milliliter of 1.5% sodium hydroxide to extract goat's horn albumen 3 hours, centrifugal removing slag in 30 ℃.Supernatant liquor is regulated pH5.6 with hydrochloric acid and is made protein precipitation.The centrifugal liquid that goes, residue are dissolved in 300 ml waters, regulate pH to 6.5, add 500000U 1398 proteolytic enzyme, 35 ℃ of insulated and stirred, and enzymolysis 5 hours, centrifugal, discard precipitation, keep supernatant liquor.Above-mentioned supernatant liquor is warming up to 80～85 ℃, the enzyme 8 minutes of going out, concentrated, spraying drying gets goat's horn protein peptide product, and in its product, the daltonian peptide content of molecular weight 1000-3000 reaches 67.4%.
Embodiment 3
Get 100 grams and cross the degreasing goat's horn powder of 200 mesh sieves, add 300 milliliter of 1.5% sodium hydroxide to extract goat's horn albumen 5 hours, centrifugal removing slag in 30 ℃.Supernatant liquor is regulated pH5.6 with hydrochloric acid and is made protein precipitation.The centrifugal liquid that goes, residue are dissolved in 300 ml waters, regulate pH to 6.5, add 500000U 1398 proteolytic enzyme, 35 ℃ of insulated and stirred, and enzymolysis 6 hours, centrifugal, discard precipitation, keep supernatant liquor.Above-mentioned supernatant liquor is warming up to 80～85 ℃, the enzyme 8 minutes of going out, concentrated, spraying drying gets goat's horn protein peptide product, and in its product, the daltonian peptide content of molecular weight 1000-3000 reaches 68.0%.
Claims (4)
1. the preparation method of a goat's horn protein peptide gets goat's horn outwash surface, drains, and wears into fine powder, crosses 200 mesh sieves, with above-mentioned goat's horn powder, measures the normal hexane soaking at room temperature 24 hours with 2 times, repeats once, sloughs normal hexane and obtains degreasing goat's horn powder.Extracted goat's horn albumen 2-5 hour, centrifugal removing slag with 3 times of amount 1.5% sodium hydroxide in 30 ℃.Supernatant liquor is regulated pH5.6 with hydrochloric acid and is made protein precipitation.The centrifugal liquid that goes, residue is water-soluble, transfers pH to 6.5, adds 1398 proteolytic enzyme in the ratio of 5000U/g substrate, 35 ℃ of insulated and stirred, enzymolysis 4～6 hours, centrifugal, discard precipitation, keep supernatant liquor.Above-mentioned supernatant liquor is warming up to 80～85 ℃, the enzyme 8～12 minutes of going out, concentrated, spraying drying gets goat's horn protein peptide product, and in its product, the daltonian peptide content of molecular weight 1000-3000 reaches more than 65%.
2. according to method claimed in claim 1, it is characterized in that raw material used for the goat's horn through degreasing, obtains the goat's horn protein peptide product of 1000-3000 dalton peptide content more than 65%.
3. according to method claimed in claim 1, when it is characterized in that enzymolysis, enzyme used is 1398 proteolytic enzyme.
4. according to method claimed in claim 1, the pH when it is characterized in that utilizing Acid precipitation goat's horn albumen is 5-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110342968 CN103088095A (en) | 2011-11-02 | 2011-11-02 | Preparation method of sheep horn protein peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110342968 CN103088095A (en) | 2011-11-02 | 2011-11-02 | Preparation method of sheep horn protein peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103088095A true CN103088095A (en) | 2013-05-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110342968 Pending CN103088095A (en) | 2011-11-02 | 2011-11-02 | Preparation method of sheep horn protein peptide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981247A (en) * | 2014-06-03 | 2014-08-13 | 中国农业科学院农产品加工研究所 | Preparation method of keratin |
| CN111888322A (en) * | 2020-09-18 | 2020-11-06 | 成都清科生物科技有限公司 | Sheep placenta extracting solution with whitening function and extraction method and application thereof |
| CN113004387A (en) * | 2019-12-19 | 2021-06-22 | 中国医学科学院药物研究所 | Sheep keratin, preparation method thereof, pharmaceutical composition thereof and application |
| CN115228131A (en) * | 2022-07-15 | 2022-10-25 | 浙江松寿堂医药有限公司 | Method for extracting and producing animal polypeptide by using sheep horn |
-
2011
- 2011-11-02 CN CN 201110342968 patent/CN103088095A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981247A (en) * | 2014-06-03 | 2014-08-13 | 中国农业科学院农产品加工研究所 | Preparation method of keratin |
| CN103981247B (en) * | 2014-06-03 | 2017-01-04 | 中国农业科学院农产品加工研究所 | A kind of keratic preparation method |
| CN113004387A (en) * | 2019-12-19 | 2021-06-22 | 中国医学科学院药物研究所 | Sheep keratin, preparation method thereof, pharmaceutical composition thereof and application |
| CN111888322A (en) * | 2020-09-18 | 2020-11-06 | 成都清科生物科技有限公司 | Sheep placenta extracting solution with whitening function and extraction method and application thereof |
| CN111888322B (en) * | 2020-09-18 | 2022-09-13 | 成都清科生物科技有限公司 | Sheep placenta extract with whitening function and extraction method and application thereof |
| CN115228131A (en) * | 2022-07-15 | 2022-10-25 | 浙江松寿堂医药有限公司 | Method for extracting and producing animal polypeptide by using sheep horn |
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Application publication date: 20130508 |