CN103088091A - High-efficiency and energy-saving validamycin fermenting method - Google Patents

High-efficiency and energy-saving validamycin fermenting method Download PDF

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Publication number
CN103088091A
CN103088091A CN201310010562XA CN201310010562A CN103088091A CN 103088091 A CN103088091 A CN 103088091A CN 201310010562X A CN201310010562X A CN 201310010562XA CN 201310010562 A CN201310010562 A CN 201310010562A CN 103088091 A CN103088091 A CN 103088091A
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fermentation
energy
dissolved oxygen
jingganmycin
efficient
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李忠
乐峰松
殷红福
陈明兆
叶全标
胡颖亮
吴闯
赵晓春
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ZHEJIANG TONGLU HUIFENG BIOCHEMISTRY CO Ltd
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ZHEJIANG TONGLU HUIFENG BIOCHEMISTRY CO Ltd
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Abstract

The invention discloses a high-efficiency and energy-saving validamycin fermenting method, belonging to the field of microbial fermentation technology. The method comprises the following technological steps: 1) inoculating streptomyces hygroscopicus strains into liquid fermentation culture medium and controlling the inoculum size to be 8-15%, wherein the fermentation temperature is at 35-45 DEG C; and 2) controlling the ventilator capacity to be 2500-3500m<3>/h at early fermentation stage, fermenting for 25-35h, and then controlling the dissolved oxygen to be 20-50% until the fermentation is finished. According to the method, the dissolved oxygen rule can be discovered by measuring and analyzing the dissolved oxygen, and the concentration of the dissolved oxygen is controlled to be 20-50% after the fermentation culture is conducted for 25-35h, so that the energy consumption of an air compressor can be effectively reduced, the total air usage amount can be saved by about 25%, the electricity consumption can be saved by 10% of total electricity consumption each year, and the production volume of the validamycin can be improved by more than 4%.

Description

A kind of energy-efficient jingganmycin fermentation process
Technical field
The invention belongs to the microbial fermentation technology field, be specifically related to a kind of energy-efficient jingganmycin fermentation process.
Background technology
Jingganmycin is the microbiotic that the Jinggangmycin bacterium produces, and is mainly used in preventing and treating rice sheath blight disease.Liquid submerged fermentation is adopted in jingganmycin production, dissolved oxygen is control index important in liquid culturing process, oxygen solubleness in fermented liquid is very little, need ceaselessly to lead to oxygen and guarantee oxygen concentration in fermented liquid, the method for controlling dissolved oxygen is improved mixing speed, adjusts air input, free air delivery etc.
Jingganmycin fermenting process energy consumption is larger.In liquid culturing process, fermentation different steps bacterial metabolism intensity is different, therefore aerobic difference.Earlier fermentation, thalline is in growth phase, and oxygen depletion is larger, and the fermentation middle and later periods, thalline is in the meta-bolites stage that produces, and the demand of oxygen is reduced relatively.If air flow is not adjusted, may cause the waste of dissolved oxygen, too high dissolved oxygen can produce singlet (O), super-oxide base (O 2-) or hydroxyl radical free radical (OH -), destroy many cellular components, cause mycelia early ageing, may affect the output of meta-bolites.
Summary of the invention
For the problem that prior art exists, the object of the invention is to design the technical scheme that a kind of energy-efficient jingganmycin fermentation process is provided.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that comprising following processing step:
1) with the Jinggangmycin inoculation to liquid fermentation medium, controlling inoculum size is 8～15%, leavening temperature is 35～45 ℃;
2) fermentation initial stage control air flow is 2500～3500m 3/ h, after fermentation 25～35h, controlling dissolved oxygen amount is 20～50%, until fermentation ends.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that containing in liquid fermentation medium in described step 1) rice meal 70～150g, groundnut meal 10～30g, NaCl1～3g, CaCO 31～3g ., KH 2PO 41～3g, the pH value is 6～9.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that controlling inoculum size in described step 1) is 10～12%.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that in described step 1), the controlled fermentation temperature is 37～42 ℃.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that described step 2) in control mixing speed 50～150r/min in fermenting process.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that described step 2) in to control air flow be 2700～3200m the fermentation initial stage 3/ h.
Described a kind of energy-efficient jingganmycin fermentation process is characterized in that described step 2) in after fermentation 27～32h, controlling dissolved oxygen amount is 25～45%.
In the present invention, Jinggangmycin bacterial strain used is Jinggangmycin 5008 bacterial strains (Streptomyces hygroscopicus var.jinggangensis 5008), and it is existing bacterial strain.
The present invention is by measurement, analysis to dissolved oxygen, find out the dissolved oxygen rule, after fermentation culture 25～35h, control oxyty 20～50%, effectively reduce the energy consumption of air compressor, can save 25% left and right of total air consumption, but annual also save electricity accounts for 10% of total electric weight, and jingganmycin output improves more than 4%.
Description of drawings
Fig. 1 is 50m 3Dissolved oxygen in fermentor tank (DO) curve;
Fig. 2 is for regulating air flow and the process parameter of not regulating air flow;
Fig. 3 is energy efficient comparison diagram of the present invention.
Embodiment
Further illustrate the present invention below in conjunction with embodiment.
Embodiment 1
1) with the Jinggangmycin inoculation to liquid fermentation medium, controlling inoculum size is 12%, leavening temperature is 40 ℃; Liquid fermentation medium contains rice meal 120g, groundnut meal 20g, NaCl2g, CaCO 31g ., KH 2PO 41g, the pH value is 7;
2) fermentation initial stage control air flow is 3000m 3/ h, 30h is to fermentation ends in fermentation, and controlling dissolved oxygen amount is 30%, controls mixing speed 120r/min in fermenting process.
Embodiment 2
1) with the Jinggangmycin inoculation to liquid fermentation medium, controlling inoculum size is 8%, leavening temperature is 35 ℃; Liquid fermentation medium contains rice meal 70g, groundnut meal 10g, NaCl1g, CaCO 31g ., KH 2PO 41g, the pH value is 6;
2) fermentation initial stage control air flow is 2500m 3/ h, 25h is to fermentation ends in fermentation, and controlling dissolved oxygen amount is 20%, controls mixing speed 50r/min in fermenting process.
Embodiment 3
1) with the Jinggangmycin inoculation to liquid fermentation medium, controlling inoculum size is 15%, leavening temperature is 45 ℃; Liquid fermentation medium contains rice meal 150g, groundnut meal 30g, NaCl2g, CaCO 32g ., KH 2PO 43g, the pH value is 8;
2) fermentation initial stage control air flow is 3500m 3/ h, 35h is to fermentation ends in fermentation, and controlling dissolved oxygen amount is 50%, controls mixing speed 150r/min in fermenting process.
According to embodiment 1, get fermentor tank A, fermentor tank B and carry out following test according to the step of embodiment 1 record.
Test example 1
Measure fermenting process dissolved oxygen curve in fermentor tank A and B.
As can be seen from Figure 1, the fermentation dissolved oxygen has following features:
0～2 hour, mycelia was less, and respiratory intensity is lower, and dissolved oxygen is higher.
2～30 hours, it is many that mycelia becomes, and respiratory intensity is higher, and dissolved oxygen is lower.
30～fermentation ends, the respiratory intensity step-down, dissolved oxygen is higher.
The 1 explanation fermentation middle and later periods of test example, the pressurized air waste is more, is necessary to carry out the air flow regulation and control.
Test example 2
Measure single tank output of each tank in fermentor tank A, B, the collimation of checking tank, test triplicate, result such as following table.
Table 1: tank A and tank B jingganmycin output (Kg)
Figure 292309DEST_PATH_IMAGE001
Test example 2 shows, fermentor tank A, B poor show that apart from not obvious in the situation that culture condition is consistent, the collimation of two tanks is better.
Test example 3
The fermentation initial stage, maximum (3000m is reached in fermentor tank A, fermentor tank B ventilation 3/ h).Fermentor tank A does not adjust, until fermentation ends, fermentor tank B reduced air flow after 30 hours, made dissolved oxygen be adjusted into 30%, until fermentation ends, measure each tank amino nitrogen, total reducing sugar, reducing sugar, tire, and mapping obtains Fig. 2.
As can be seen from Figure 2, compare with not regulating air flow, the value of adjusting amino nitrogen, total reducing sugar, the reducing sugar after air flow, tire (ug/ml) is similar, illustrate reduce ventilate little to the fermentating metabolism process influence.
Test example 4
The fermentation initial stage, maximum (3000m is reached in fermentor tank A, fermentor tank B ventilation 3/ h).Fermentor tank A does not adjust, until fermentation ends, fermentor tank B 30 and after 35 hours reduces air flow, makes dissolved oxygen be adjusted into 30%, until fermentation ends, the record two total air flows of tank fermenting process calculate air flow saving amount.
The total air flow of air flow saving amount=fermentor tank A-total air flow of fermentor tank B
Table 2: after adjusting air flow, air flow is saved situation (m 3)
Figure 209449DEST_PATH_IMAGE002
Test example 4 shows, after adjusting air flow, can save a large amount of pressurized air, thereby reduces the air compressor machine opening time, effectively reduces energy consumption.
Test example 5
The fermentation initial stage, maximum (3000m is reached in fermentor tank A, fermentor tank B ventilation 3/ h).Fermentor tank A does not adjust, until fermentation ends, fermentor tank B reduced air flow after 30 hours, made dissolved oxygen be adjusted into 30%, until fermentation ends is measured single tank output, test repeats 3 times, result such as following table.
Show to begin to adjust in 3:30 hour dissolved oxygen list tank output (kg)
Figure 926870DEST_PATH_IMAGE003
In table, the jingganmycin output of tank B is higher by 5.2% than tank A, illustrates that 30h begins to regulate and control dissolved oxygen jingganmycin output is increased, and air flow reduces greatly simultaneously, and energy consumption reduces greatly.
Test example 6
The fermentation initial stage, maximum (3000m is reached in fermentor tank A, fermentor tank B ventilation 3/ h).Fermentor tank A does not adjust, until fermentation ends, fermentor tank B reduced air flow after 35 hours, made dissolved oxygen be adjusted into 30%, until fermentation ends is measured single tank output, test repeats 3 times, result such as following table.
Show to begin to adjust in 4:35 hour air flow list tank output
In table, the jingganmycin output of tank B is higher by 4.8% than tank A, illustrates that 35h begins to regulate and control dissolved oxygen jingganmycin output is increased, and air flow reduces greatly simultaneously, and energy consumption reduces greatly.
Test example 7
The jingganmycin fermentation process that carries out according to embodiment 1 with do not have the control by stages air flow to carry out jingganmycin fermentation process energy saving comparison, as shown in Figure 3.
Can obtain from Fig. 3, carry out the jingganmycin fermentation by method of the present invention, can effectively reduce current consumption, accomplish energy-saving and emission-reduction.
Carry out the identical test of test example 1-7 according to embodiment 2 with 3 condition, it also can reach the technique effect identical with embodiment 1 at last.

Claims (7)

1. energy-efficient jingganmycin fermentation process is characterized in that comprising following processing step:
1) with the Jinggangmycin inoculation to liquid fermentation medium, controlling inoculum size is 8～15%, leavening temperature is 35～45 ℃;
2) fermentation initial stage control air flow is 2500～3500m 3/ h, after fermentation 25～35h, controlling dissolved oxygen amount is 20～50%, until fermentation ends.
2. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that containing in liquid fermentation medium in described step 1) rice meal 70～150g, groundnut meal 10～30g, NaCl1～3g, CaCO 31～3g ., KH 2PO 41～3g, the pH value is 6～8.
3. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that controlling inoculum size in described step 1) is 10～12%.
4. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that in described step 1), the controlled fermentation temperature is 37～42 ℃.
5. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that described step 2) in control mixing speed 50～150r/min in fermenting process.
6. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that described step 2) in to control air flow be 2700～3200m the fermentation initial stage 3/ h.
7. a kind of energy-efficient jingganmycin fermentation process as claimed in claim 1, is characterized in that described step 2) in after fermentation 27～32h, controlling dissolved oxygen amount is 25～45%.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937856A (en) * 2014-04-21 2014-07-23 浙江大学 Fermentation method capable of enhancing validamycin yield
CN105754890A (en) * 2015-12-21 2016-07-13 武汉科诺生物科技股份有限公司 Streptomyces hygroscopicus for producing validamycin and application of streptomyces hygroscopicus
CN106434795A (en) * 2016-09-19 2017-02-22 浙江大学 Method for increasing validamycin yield by pH shock
CN110358798A (en) * 2019-08-21 2019-10-22 浙江省桐庐汇丰生物科技有限公司 A kind of method of fermenting and producing jinggangmeisu
CN111647535A (en) * 2020-06-18 2020-09-11 浙江省桐庐汇丰生物科技有限公司 Streptomyces hygroscopicus mutant strain for producing validamycin and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101130562A (en) * 2007-09-14 2008-02-27 浙江工业大学 Method for preparing high purity validamycin powder agent
CN101280283A (en) * 2008-05-29 2008-10-08 鲁南制药集团股份有限公司 Production method of tacrolimus
CN102191302A (en) * 2011-04-10 2011-09-21 浙江钱江生物化学股份有限公司 Production method for improving validamycin fermentation level
CN102732460A (en) * 2012-06-13 2012-10-17 浙江师范大学 Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101130562A (en) * 2007-09-14 2008-02-27 浙江工业大学 Method for preparing high purity validamycin powder agent
CN101280283A (en) * 2008-05-29 2008-10-08 鲁南制药集团股份有限公司 Production method of tacrolimus
CN102191302A (en) * 2011-04-10 2011-09-21 浙江钱江生物化学股份有限公司 Production method for improving validamycin fermentation level
CN102732460A (en) * 2012-06-13 2012-10-17 浙江师范大学 Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937856A (en) * 2014-04-21 2014-07-23 浙江大学 Fermentation method capable of enhancing validamycin yield
CN103937856B (en) * 2014-04-21 2016-06-08 浙江大学 A kind of fermentation process improving jingganmycin yield
CN105754890A (en) * 2015-12-21 2016-07-13 武汉科诺生物科技股份有限公司 Streptomyces hygroscopicus for producing validamycin and application of streptomyces hygroscopicus
CN105754890B (en) * 2015-12-21 2019-08-02 武汉科诺生物科技股份有限公司 One plant of streptomyces hygroscopicus for producing jinggangmeisu and its application
CN106434795A (en) * 2016-09-19 2017-02-22 浙江大学 Method for increasing validamycin yield by pH shock
CN106434795B (en) * 2016-09-19 2020-02-18 浙江大学 Method for improving validamycin yield through pH impact
CN110358798A (en) * 2019-08-21 2019-10-22 浙江省桐庐汇丰生物科技有限公司 A kind of method of fermenting and producing jinggangmeisu
CN111647535A (en) * 2020-06-18 2020-09-11 浙江省桐庐汇丰生物科技有限公司 Streptomyces hygroscopicus mutant strain for producing validamycin and application thereof

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Application publication date: 20130508