CN103088085B - Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts - Google Patents
Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts Download PDFInfo
- Publication number
- CN103088085B CN103088085B CN201210594838.9A CN201210594838A CN103088085B CN 103088085 B CN103088085 B CN 103088085B CN 201210594838 A CN201210594838 A CN 201210594838A CN 103088085 B CN103088085 B CN 103088085B
- Authority
- CN
- China
- Prior art keywords
- pulullan polysaccharide
- culture medium
- starch
- root
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a method for fermenting pulullan polysaccharide by preparing a culture medium from starch wastewater and malt sprouts. According to the method for fermenting pulullan polysaccharide by preparing a culture medium from starch wastewater and malt sprouts, the preparation for the culture medium comprises the following steps of: preparing a culture medium, activating bacterial strains, fermenting and cultivating, treating by alcohol, and drying to a obtain white or light yellow polysaccharide crude product. The preparation method for the culture medium comprises the following steps of: (1), crushing and sieving the malt sprouts to prepare malt sprout juice; and (2), mixing the malt sprout juice with industrial starch wastewater, and dipping; controlling the temperature to carry out protein (Pr) resting; controlling temperature to carry out saccharifying; heating up, filtering, washing the slot, adjusting the pH, adding inorganic salt and sterilizing. According to the method disclosed by the invention, the pulullan polysaccharide is prepared by utilizing industrial waste-starch industrial wastewater, so that the waste material is changed into things of value, the production cost of the pulullan polysaccharide is greatly reduced, and the pollution, which is caused by the starch industrial wastewater, to the environment is reduced, thereby achieving the win-win effects of the resources and the environment.
Description
Technical field
The present invention relates to a kind of method of the pulullan polysaccharide that ferments, be specifically related to a kind ofly with starch wastewater and root of Cornu Cervi Pantotrichum, prepare the ferment method of pulullan polysaccharide of substratum.
Background technology
Pulullan polysaccharide is the Microbial exopolysaccharides that a class has significant application value, and film-forming properties, one-tenth fiber, gas barrier property, Biosafety and nontoxic feature, make it in food, medicine, chemical industry and oil field, be used widely.
Starch wastewater is inevitable a kind of by product in starch producing process, nutritious; Root of Cornu Cervi Pantotrichum is the by product in Fructus Hordei Germinatus manufacturing processed, contains abundant amylase, maltase, tartaric acid enzyme and proteolytic enzyme, is rich in vitamin B group, and contains a large amount of UGFs.
What the carbon source of the pulullan polysaccharide fermention medium of reporting in document was at present more is starch or the sucrose using after fire resistant alpha-diastase enzymolysis, and expensive, complex process, has increased pulullan polysaccharide fermentation costs.In addition lack at present pigment formation and lack and the high bacterial strain of transposon mutagenesis ability, these all impel the rising of pulullan polysaccharide holistic cost.
Summary of the invention
In order to solve the above-mentioned problem due to technical deficiency generation, further reduce the cost of pulullan polysaccharide, the object of the present invention is to provide and a kind ofly with starch wastewater and root of Cornu Cervi Pantotrichum, prepare the ferment method of pulullan polysaccharide of substratum, raw material sources are extensive, utilize root of Cornu Cervi Pantotrichum contained enrich enzyme system and somatomedin is carried out enzymolysis wastewater from starch industry, substratum is simple, has both improved polysaccharide yield, reduce polysaccharide production cost, also solved the environmental problem of being brought by starch wastewater and root of Cornu Cervi Pantotrichum.In addition, the bacterial strain pigment formation ability of cultivating by the method obviously declines, and has reduced this step of decolouring, greatly reduces the cost of pulullan polysaccharide.
To achieve these goals, the technical solution used in the present invention is: a kind ofly with starch wastewater and root of Cornu Cervi Pantotrichum, prepare the ferment method of pulullan polysaccharide of substratum, comprise the following steps:
Described substratum preparation method is as follows:
(1) root of Cornu Cervi Pantotrichum is pulverized, crossed 20 mesh sieves, preparation root of Cornu Cervi Pantotrichum juice, mass concentration 20%-50%;
(2) root of Cornu Cervi Pantotrichum juice is added and mixed according to mass ratio 20～40:100 with industrial starch waste water, at 38～40 ℃ of dipping 25-40min; Control temperature and be 45 ℃ and carry out protein (Pr) and stop, keep 20-40min, control temperature and be 55 ℃ and carry out for the second time protein and stop and keep 30 minutes, control temperature and at 63 ℃, carry out first paragraph saccharification, hold-time 40min; Second segment saccharification temperature is 68 ℃, and it is colourless to iodine liquid, reacting; Be warmed up to 76～78 ℃, filter, wash grain, adjust pH to 5.6-7.4, add the NaCl of 2～3g/L, the MgSO of 0.2～0.4g/L
4, 3～5g/L K
2hPO
4, sterilizing becomes substratum.
Described fermentation culture method is as follows:
(1) Aureobasidium pullulans (Aureobsidium pullulans) CGMCCNO.7055 bacterial strain is activated, being about to it is transferred in the baffle plate bottle of 500ml from sucrose nutrient agar inclined-plane, the bottled liquid measure of baffle plate is 80ml, at 28 ℃ of rotating speed 180r/min of constant temperature, cultivates 30h.
(2) with 4% volume ratio, (1) gained kind daughter bacteria liquid is transferred in the baffle plate bottle of 500ml substratum, the bottled liquid measure of baffle plate is 100ml, at 28 ℃ of rotating speed 200r/min of constant temperature, cultivates after 84～104h, gets the centrifugal removal mycelium of fermented liquid.
(3) in supernatant liquid, add the long-pending dehydrated alcohol of triploid, stir, 4 ℃ of placements are spent the night, pulullan polysaccharide is fully precipitated, precipitated liquid obtains pulullan polysaccharide precipitation with the centrifugal 30min of 5000r/min, precipitation absolute ethanol washing 2 times, dry white or the light yellow polysaccharide crude of obtaining.
Advantage of the present invention is as follows:
The present invention utilizes trade waste-wastewater from starch industry to carry out the preparation of pulullan polysaccharide, can not only turn waste into wealth, greatly reduce the production cost of pulullan polysaccharide, also can reduce the pollution that wastewater from starch industry causes environment, played the effect of resources and environment doulbe-sides' victory.
The present invention has utilized the enzyme system that enriches in the by product-root of Cornu Cervi Pantotrichum of malthouse to replace lytic enzyme to carry out the Starch Hydrolysis in waste water, also utilize the growth metabolism of abundant somatomedin promotion aureobasidium pullulans in root of Cornu Cervi Pantotrichum, improved the output of pulullan polysaccharide.
The bacterial strain pigment formation of cultivating through the present invention obviously reduces, thereby reduces product decolouring step, greatly reduces cost.
Concrete embodiment
Below in conjunction with specific examples, the present invention is further illustrated.
Embodiment 1
With starch wastewater and root of Cornu Cervi Pantotrichum, prepare the ferment method of pulullan polysaccharide of material, comprise the following steps:
One, root of Cornu Cervi Pantotrichum is pulverized, crossed 20 mesh sieves, preparation root of Cornu Cervi Pantotrichum juice concentration 50%.
Two, root of Cornu Cervi Pantotrichum juice is mixed → floods (38～40 ℃ with industrial starch waste water according to the interpolation of mass ratio 20:100, stop (45 ℃ of I of 30min) → Pr, stop (55 ℃ of II of 30min) → Pr, 30min) → (63 ℃ of first paragraph saccharification, 40min) → (68 ℃ of second segment saccharification, to the reaction of iodine liquid, for colourless → (76～78 ℃) → filtration → washing trough → adjusting pH to 5.8 that heats up, add the NaCl of 2g/L, the MgSO of 0.2g/L
4, 3g/L K
2hPO
4through sterilizing, become substratum.
Three, by CGMCCNO.7055 activation, be about to it and be transferred in the baffle plate bottle of 500ml from sucrose nutrient agar inclined-plane, the bottled liquid measure of baffle plate is 80ml, at 28 ℃ of rotating speed 180r/min of constant temperature, cultivates 30h.
Three, with 4% volume ratio, kind of daughter bacteria liquid is transferred in the baffle plate bottle of 500ml, the bottled liquid measure of baffle plate is 100ml, at 28 ℃ of rotating speed 200r/min of constant temperature, cultivates after 84h, gets the centrifugal removal mycelium of fermented liquid.
Four, in supernatant liquid, add the long-pending dehydrated alcohol of triploid, stir, 4 ℃ of placements are spent the night, pulullan polysaccharide is fully precipitated, precipitated liquid obtains pulullan polysaccharide precipitation with the centrifugal 30min of 5000r/min, precipitation absolute ethanol washing 2 times, dry white or light yellow polysaccharide crude, the output 98g/L of obtaining.
Embodiment 2
One, root of Cornu Cervi Pantotrichum is pulverized, crossed 20 mesh sieves, preparation root of Cornu Cervi Pantotrichum juice concentration 30%.
Two, root of Cornu Cervi Pantotrichum juice is added and mixes → flood (38～40 ℃ according to mass ratio 30:100 with industrial starch waste water, stop (45 ℃ of I of 30min) → Pr, stop (55 ℃ of II of 30min) → Pr, 30min) → (63 ℃ of first paragraph saccharification, 40min) → (68 ℃ of second segment saccharification, it is colourless to iodine liquid, reacting) → (76～78 ℃) → filtration → washing trough → adjusting pH to 6.6 that heats up, adds the NaCl of 3g/L, the MgSO of 0.3g/L
4, 4g/L K
2hPO
4through sterilizing, become substratum.
Three, by CGMCCNO.7055 activation, be about to it and be transferred in the baffle plate bottle that 500ml is equipped with above-mentioned substratum from sucrose nutrient agar inclined-plane, the bottled liquid measure of baffle plate is 80ml, at 28 ℃ of rotating speed 180r/min of constant temperature, cultivates 30h.
Three, with 4% volume ratio, kind of daughter bacteria liquid is transferred in the baffle plate bottle that 500ml is equipped with above-mentioned substratum, the bottled liquid measure of baffle plate is 100ml, at 28 ℃ of rotating speed 200r/min of constant temperature, cultivates after 96h, gets the centrifugal removal mycelium of fermented liquid.
Four, in supernatant liquid, add the long-pending dehydrated alcohol of triploid, stir, 4 ℃ of placements are spent the night, pulullan polysaccharide is fully precipitated, precipitated liquid obtains pulullan polysaccharide precipitation with the centrifugal 30min of 5000r/min, precipitation absolute ethanol washing 2 times, dry white or light yellow polysaccharide crude, the output 105g/L of obtaining.
Embodiment 3
One, root of Cornu Cervi Pantotrichum is pulverized, crossed 20 mesh sieves, preparation root of Cornu Cervi Pantotrichum juice concentration 20%.
Two, root of Cornu Cervi Pantotrichum juice is added and mixes → flood (38～40 ℃ according to mass ratio 40:100 with industrial starch waste water, stop (45 ℃ of I of 30min) → Pr, stop (55 ℃ of II of 30min) → Pr, 30min) → (63 ℃ of first paragraph saccharification, 40min) → (68 ℃ of second segment saccharification, it is colourless to iodine liquid, reacting) → (76～78 ℃) → filtration → washing trough → adjusting pH to 7.1 that heats up, adds the NaCl of 2g/L, the MgSO of 0.4g/L
4, 5g/L K
2hPO
4through sterilizing, become nutrient solution.
Three, by CGMCCNO.7055 activation, be about to it and be transferred in the baffle plate bottle of 500ml from sucrose nutrient agar inclined-plane, the bottled liquid measure of baffle plate is 80ml, at 28 ℃ of rotating speed 180r/min of constant temperature, cultivates 30h.
Three, with 4% volume ratio, kind of daughter bacteria liquid is transferred in the baffle plate bottle of 500ml, the bottled liquid measure of baffle plate is 100ml, at 28 ℃ of rotating speed 200r/min of constant temperature, cultivates after 104h, gets the centrifugal removal mycelium of fermented liquid.
Four, in supernatant liquid, add the long-pending dehydrated alcohol of triploid, stir, 4 ℃ of placements are spent the night, pulullan polysaccharide is fully precipitated, precipitated liquid obtains pulullan polysaccharide precipitation with the centrifugal 30min of 5000r/min, precipitation absolute ethanol washing 2 times, dry white or light yellow polysaccharide crude, the output 102g/L of obtaining.
One strain Aureobasidium pullulans (Aureobsidium pullulans), this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 25th, 2012, address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number is CGMCCNO.7055
1. the acquisition of mutagenic strain
(1) select well-grown Aureobasidium pullulans bacterial strain TKPM10017 slant strains, by stroke-physiological saline solution, wash lower spore, manufacture the spore suspension of 106/mL, under 30W ultraviolet lamp, 30cm place, irradiate respectively 0.5min, 1min, 1.5min, 2min, 2.5min3min, 3.5min, 4min by spore suspension gradient dilution painting flat board, 28 ℃ of cultivations of lucifuge, calculate lethality rate;
(2) select the irradiation time of 0.5min, 2min, tri-various dose of 4min respectively the spore suspension of bacterial strain TKPM10017 to be carried out to uv irradiating processing;
(3) then the spore suspension of three different irradiation times is mixed, carry out 10-1～10-6 gradient dilution, get 10
-4, 10
-5, 10
-6three dilution spore suspension spread plates, 28 ℃ of lucifuges are cultivated 3d;
(4) mutagenic strain in (3) is inoculated in the seed culture medium that contains 10mg/L oligomycin and is cultivated; Seed liquor is placed in to 28 ℃ of constant-temperature tables, 200r/min, shaking culture 3d;
(5) seed liquor in (4) is carried out to gradient dilution, get 10
-4, 10
-5, 10
-6three dilution spore suspensions coating slant mediums are dull and stereotyped, cultivate 3d for 28 ℃, and color oyster white in screening culture medium, bacterium colony is large and thickness, moistening bacterial strain.
(6) again the bacterial strain of screening in (5) is inoculated into respectively in seed culture medium, at 28 ℃, under 200r/min, shaking culture 36h, in 5% inoculum size access fermention medium, 28 ℃, 200r/min bottom fermentation 3d are centrifugal, the content that anthrone sulfuric acid process is measured pulullan polysaccharide in supernatant liquor, sifts out pulullan polysaccharide superior strain BCSWGHPL101 again.
2. the characteristic of mutagenic strain BCSWGHPL101
(1) colonial morphology: in fermented liquid, mainly present yeast sample form, oval and big or small homogeneous, the modes of reproduction of bacterial strain is similar to the gemmation mode of yeast.
(2) colonial morphology: bacterium colony is rounded, central elevation, smooth surface, moistening, be difficult for picking, the growth later stage has mycelium to produce.
(3) cultural characteristic: 28 ℃, under 200rpm, 3d is cultivated in concussion, and fermented liquid color is milky white to milk yellow.
(4) utilizable carbon source: glucose, sucrose, maltose, fructose, Zulkovsky starch, dextrin one of them.
(5) utilizable nitrogenous source: extractum carnis, peptone, corn steep liquor, SODIUMNITRATE, yeast extract paste, ammonium sulfate, ammonium nitrate etc., above-mentioned nitrogenous source can mix use, also can singlely use.
(6) can be in the situation that 10mg/L oligomycin exists well-grown.
1. the configuration of substratum:
(1) strain activation and culture base: potato glucose agar medium (PDA): peeling potato 200g/L, add glucose 20g/L, agar 20g/L,, pH nature.121 ℃ of 20min sterilizings.
(2) seed culture medium: peptone 3g/L, sucrose 50g/L, K
2hPO
47H
2o2.0g/L, MgSO
47H
2o0.4g/L, NaCl2.5g/L, regulates pH to 7.0, and 121 ℃ of autoclaving 20min are standby.
(3) fermention medium: peptone 3g/L, sucrose 100g/L, K
2hPO
4.7H
2o3.0g/L, MgSO
47H
2o 0.4g/L, NaCl2.5g/L, FeSO
40.01g/L regulates pH to 7.0, and 121 ℃ of autoclaving 20min are standby.
(4) select substratum: oligomycin 5mg/L (sterilizing separately), peptone 3g/L, sucrose 50g/L, K
2hPO
47H
2o2.0g/L, MgSO
47H
2o0.4g/L, NaCl2.5g/L, regulates pH to 7.0, and 121 ℃ of autoclaving 20min are standby.
(5) primary dcreening operation substratum is PDA substratum: peeling potato 200g/L, adds glucose 20g/L, agar 20g/L, pH nature.121 ℃ of 20min sterilizings.
Claims (1)
1. the method that the substratum that utilizes starch wastewater and root of Cornu Cervi Pantotrichum to prepare ferments, comprises the steps:
(1) Aureobasidium pullulans (Aureobsidium pullulans) CGMCCNO.7055 bacterial strain is activated;
(2) with 4% volume ratio, kind of daughter bacteria liquid is transferred in the baffle plate bottle of 500ml substratum, the bottled liquid measure of baffle plate is 100ml, at 28 ℃ of rotating speed 200r/min of constant temperature, cultivates after 84～104h, gets the centrifugal removal mycelium of fermented liquid;
(3) in supernatant liquid, add the long-pending dehydrated alcohol of triploid, stir, 4 ℃ of placements are spent the night, pulullan polysaccharide is fully precipitated, precipitated liquid obtains pulullan polysaccharide precipitation with the centrifugal 30min of 5000r/min, precipitation absolute ethanol washing 2 times, dry white or the light yellow polysaccharide crude of obtaining;
Described substratum preparation method is as follows:
(1) root of Cornu Cervi Pantotrichum is pulverized, crossed 20 mesh sieves, preparation root of Cornu Cervi Pantotrichum juice, mass concentration 20%-50%;
(2) root of Cornu Cervi Pantotrichum juice is added and mixed according to mass ratio 20～40:100 with industrial starch waste water, at 38～40 ℃ of dipping 25-40min; Control temperature and be 45 ℃ and carry out protein (Pr) and stop, keep 20-40min, control temperature and be 55 ℃ and carry out for the second time protein and stop and keep 30 minutes, control temperature and at 63 ℃, carry out first paragraph saccharification, hold-time 40min; Second segment saccharification temperature is 68 ℃, and it is colourless to iodine liquid, reacting; Be warmed up to 76～78 ℃, filter, wash grain, adjust pH to 5.6-7.4, add the NaCl of 2～3g/L, the MgSO of 0.2～0.4g/L
4, 3～5g/L K
2hPO
4, sterilizing becomes substratum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210594838.9A CN103088085B (en) | 2012-12-31 | 2012-12-31 | Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210594838.9A CN103088085B (en) | 2012-12-31 | 2012-12-31 | Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103088085A CN103088085A (en) | 2013-05-08 |
| CN103088085B true CN103088085B (en) | 2014-08-27 |
Family
ID=48201212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210594838.9A Active CN103088085B (en) | 2012-12-31 | 2012-12-31 | Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103088085B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695476B (en) * | 2013-12-25 | 2016-03-02 | 天津北洋百川生物技术有限公司 | Ethanol-pulullan polysaccharide coupling fermentation method |
| JP7222911B2 (en) | 2017-04-14 | 2023-02-15 | カプスゲル・ベルギウム・ナムローゼ・フェンノートシャップ | How to make pullulan |
| AU2018251256B2 (en) | 2017-04-14 | 2023-10-05 | Capsugel Belgium Nv | Pullulan capsules |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1654482A (en) * | 2005-02-21 | 2005-08-17 | 汕头市富味制果厂有限公司 | Process for producing pullulan |
| CN101942492A (en) * | 2009-07-03 | 2011-01-12 | 山东省生物药物研究院 | Method for producing pullulan with different molecular weights |
| CN102492752A (en) * | 2011-12-16 | 2012-06-13 | 天津北洋百川生物技术有限公司 | Method for co-producing pullulan polysaccharide and melanin by Aureobasidium pullulan |
| CN102732048A (en) * | 2012-05-29 | 2012-10-17 | 天津北洋百川生物技术有限公司 | Extraction method for melanin of Aureobasidium pullulans |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5623895A (en) * | 1979-08-07 | 1981-03-06 | Agency Of Ind Science & Technol | Increase of yield of glucose |
| JPS6034182A (en) * | 1983-08-03 | 1985-02-21 | Sapporo Breweries Ltd | Novel amylase, its preparation, and preparation of maltopentaose using it |
| JPS6434296A (en) * | 1987-07-28 | 1989-02-03 | Japan Res Dev Corp | Production of starch saccharide |
-
2012
- 2012-12-31 CN CN201210594838.9A patent/CN103088085B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1654482A (en) * | 2005-02-21 | 2005-08-17 | 汕头市富味制果厂有限公司 | Process for producing pullulan |
| CN101942492A (en) * | 2009-07-03 | 2011-01-12 | 山东省生物药物研究院 | Method for producing pullulan with different molecular weights |
| CN102492752A (en) * | 2011-12-16 | 2012-06-13 | 天津北洋百川生物技术有限公司 | Method for co-producing pullulan polysaccharide and melanin by Aureobasidium pullulan |
| CN102732048A (en) * | 2012-05-29 | 2012-10-17 | 天津北洋百川生物技术有限公司 | Extraction method for melanin of Aureobasidium pullulans |
Non-Patent Citations (7)
| Title |
|---|
| JP昭56-23895A 1981.03.06 |
| JP昭60-34182A 1985.02.21 |
| JP昭64-34296A 1989.02.03 |
| 以淀粉废水、麦芽根制备生物材料-短梗霉多糖;邵荣等;《食品科学》;20071231;第28卷(第8期);第315页右栏第1.2.3节、第316页右栏第2.2.1节、图2 * |
| 利用麦芽根、淀粉废水高产普鲁兰多糖短梗霉菌株选育的研究;邵荣等;《食品科学》;20081231;第29卷(第9期);第356页第1.3.1-1.3.5节 * |
| 邵荣等.以淀粉废水、麦芽根制备生物材料-短梗霉多糖.《食品科学》.2007,第28卷(第8期),第315页右栏第1.2.3节、第316页右栏第2.2.1节、图2. |
| 邵荣等.利用麦芽根、淀粉废水高产普鲁兰多糖短梗霉菌株选育的研究.《食品科学》.2008,第29卷(第9期),全文. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103088085A (en) | 2013-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103060204B (en) | Mutant strain for mass production of pulullan and cultural method thereof | |
| CN108865951B (en) | A kind of general bacterium of camel thorn and its microbial inoculum and application | |
| CN103421718B (en) | A kind of sphingomonas paucimobilis bacterial strain and application thereof | |
| CN111362751B (en) | Special compound microbial disease-resistant growth-promoting bacterial fertilizer for ginseng and preparation method thereof | |
| CN102533718A (en) | Composite bacterial agent for efficiently degrading kitchen garbage, and preparation method and application thereof | |
| CN106222098A (en) | One strain monascus sp bacteria strain and application thereof | |
| CN102653724B (en) | Lactobacillus casei and application thereof to produce L-lactic acid by fermentation | |
| CN102703342B (en) | Bacillus velezensis ZJ20 strain and liquid preparations thereof | |
| CN103333845B (en) | Pseudomonas chlororaphis and fermenting cultivation method thereof | |
| CN108401794A (en) | A kind of armillaria mellea accreting with Rhizoma Gastrodiae liquid spawn production method and cultigen special culture media | |
| CN104911169B (en) | A kind of method for training cordyceps mycelium and fibrinolysin processed | |
| CN106518389A (en) | Biofertilizer with edible fungus residue as raw material and preparation thereof | |
| CN103243135A (en) | Novel culture medium for producing pulullan and method for fermenting and producing pulullan | |
| CN105420169B (en) | Gamma-polyglutamic acid generating bacterium and the method for producing gamma-polyglutamic acid organic fertilizer | |
| CN103088085B (en) | Method for fermenting pulullan polysaccharide by preparing culture medium from starch wastewater and malt sprouts | |
| CN104673675B (en) | A kind of Green microecological compound fertilizer and preparation method thereof | |
| CN104109636B (en) | Aspergillus versicolor SD-3 and in the application prepared in chitin deacetylase | |
| CN103739319B (en) | A kind of microbial fertilizer | |
| CN105481486A (en) | Method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and obtained product | |
| CN107646523A (en) | A kind of cultural method of mushroom with abundant selenium | |
| CN104805029B (en) | A kind of preparation method of fertilizer | |
| CN103005437A (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN100390295C (en) | Microorganism polysaccharide and its preparation method and application | |
| CN102181483A (en) | Preparation method of microbial flocculant | |
| CN102533604B (en) | Brevibacterium flavum and application thereof as well as method for preparing lysine through fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170830 Address after: 300457, building 4, wing Sheng Plaza, Tianjin Binhai Zhongguancun science and Technology Park, 501 Patentee after: Tianjin Huizhi Baichuan Biological Engineering Co. Ltd. Address before: 300457 Tianjin prime Binhai Economic and Technological Development Zone, Road No. 29 Patentee before: Tianjin Peiyang Biotrans Biotech Co., Ltd. |