CN103087910A - Device and method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria - Google Patents
Device and method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria Download PDFInfo
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- CN103087910A CN103087910A CN201310021957XA CN201310021957A CN103087910A CN 103087910 A CN103087910 A CN 103087910A CN 201310021957X A CN201310021957X A CN 201310021957XA CN 201310021957 A CN201310021957 A CN 201310021957A CN 103087910 A CN103087910 A CN 103087910A
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Abstract
The invention discloses a device and a method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria. The method comprises the following steps of: arranging a device for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria; placing a certain amount of washed and filtered aerobic active sludge into a trench type biological reaction tank, and mixing the aerobic active sludge with a nutrient solution; gradually introducing the nutrient solution heated by a preheating system into the reaction tank; intercepting anaerobic ammonium oxidation bacteria through cloth-type suspension fiber filler; and after 60-90 days culture, taking out the cloth-type suspension fiber filler full of brown anaerobic ammonium oxidation bacteria, eluting and naturally precipitating to obtain a great amount of anaerobic ammonia oxidation denitrified sludge with high purity. The device and method disclosed by the invention can achieve the aims of fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria so as to provide sufficient inoculated sludge for starting an anaerobic ammonia oxidation denitrifying reactor and achieve the aim of quickly starting the oxidation reactor to remove ammonia nitrogen and nitrite nitrogen out of wastewater.
Description
Technical field
The present invention relates to Environmental Biotechnology, the apparatus and method that specifically a kind of fast enriching propagation and purifying are cultivated anaerobic ammonia oxidizing bacteria.
Background technology
The nineteen ninety-five people have just found the existence of anaerobic ammonium oxidizing bacteria first.Nitrated from people are familiar with before-the denitrification biology is fully different, this newfound ancient type bacterium is a kind of chemosynthetic autotroph bacterium, complete denitrogenation with a kind of novel pathways metabolism, under anaerobic, with ammonia nitrogen, take ammonia nitrogen as electron donor, nitrite nitrogen is as electron acceptor(EA), the final nitrogen that generates, the chemical energy discharged in this process, is used for carrying out the fixing of inorganic carbon, and completes other vital movement processes as the energy by this bacterium.Under the condition of using the Anammox denitrification process, only ammonia nitrogen only about half of in waste water need to be oxidized to nitrite nitrogen, then react and generate nitrogen and escape into atmosphere with half ammonia nitrogen of residue, thereby compared to traditional nitrated-denitrification biological denitrogenation technique, the supply that the Anammox denitrification process can reduce oxygen in a large number can complete biological denitrification process.In addition, because anaerobic ammonium oxidizing bacteria belongs to chemoautotrophy type bacterium, and ammonia nitrogen is by the nitrite nitrogen oxidation, the energy density of the reaction of common formation nitrogen is very low, the bacterial growth division is extremely slow, thereby make the Anammox denitrogenation not only not need additionally to add organic carbon source, and surplus sludge volume significantly reduces compared to traditional technology, maintaining even under many circumstances sufficient sludge quantity is a normal work to do that maintains the Anammox reaction, sometimes for obtaining excess sludge, have to adopt special measure and technology to accelerate the fast breeding of anaerobic ammonium oxidizing bacteria.
The technique that denitrification of anaerobic ammonium oxidation technique is low as a kind of working cost, efficiency is high more and more is subject to the attention of countries in the world, and has carried out large quantity research.Although first Practical Project based on anaerobic ammonia oxidation process has spent about 3 years and has finally moved successfully, on the ground such as German, Japanese, Australian, in succession also there is Practical Project to put into operation afterwards, but initial startup mud mostly derive from Holland first move the Rotterdam sewage work of this technique success, but still perplexing the research and development personnel of countries in the world around the many practical problemss in this process application.Enrichment anaerobic ammonium oxidizing bacteria carry out fast breeding and purifying the provenance of wherein very disperseing from nature, for production practice provide, quantity is large, the high reactor seed sludge of purity is first difficult problem that at first people run into.
The microorganism of the aerobic and anaerobic ferment process before anaerobic ammonia oxidation microbiological is different from, itself will spend the plenty of time enrichment of microorganism, and this bacterial multiplication speed is very slow, and the startup of reactor often needs to expend the plenty of time.Particularly running into when this bacterium is produced to the material of toxic action, biological reactions rates reduces rapidly, even stop, in this case, if can not find in time, will cause a large amount of accumulation of nitrite nitrogen, thereby bacterium is produced to toxic action, finally cause the failure of reactor operation, the fresh mud of renewed vaccination of even having to is restarted.So fast enriching, and can breed in a large number and purifying anaerobic ammoxidation activity mud, be the problem that at first this process application and research need solve.
People reach the purpose that research and production application anaerobic ammonium oxidizing bacteria remove in water too much nitrogen; at first be will be from the anaerobic ammoxidation activity mud of occurring in nature enrichment, purifying, propagation sufficient amount; with can rapid, high volume remove out the unnecessary nitrogen of high nitrogenous water, avoid with the protection receiving water body threat that body eutrophication pollutes.But the method that much research all adopts the aerobic sludge sewage of direct use, anaerobism sewage disposal granule sludge or sediment of pond to start reactor starts experimental Anammox denitrification reactor, although but now in reactor, also can carry out the Anammox denitrification process, but in reactor mud, the content of anaerobic ammonium oxidizing bacteria is all lower, and total amount of sludge is also fewer, thereby can not meet the needs of large-scale reactor to the anaerobic oxidation denitrifying activated sludge, can't be applied to produce.
Chinese patent application book CN102145940A discloses " a kind of device and method of fast breeding anaerobic ammonia oxidizing bacteria " (1), CN102061281A discloses " utilizing the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria " (2), CN101735947A discloses " a kind of soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment " (3), these methods or be conceived to the fast breeding of anaerobic ammonia oxidizing bacteria, i.e. its inoculation be a small amount of anaerobic ammonium oxidizing bacteria (1) that enriching and purifying is good; With membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria, the anaerobic ammonium oxidizing bacteria disperseed is enriched on film, improved significantly bioaccumulation efficiency, structure for membrane bioreactor there is no particular requirement, and the quantity of the anaerobic ammonium oxidizing bacteria obtained and purity have much do not guarantee (2); Or with earth pillar enrichment anaerobic ammonium oxidizing bacteria, anaerobic ammonium oxidizing bacteria is enriched in earth pillar, but exist and be difficult to the problem that the bacterium of enrichment is separated after enrichment completes, all can not obtain directly, easily the anaerobic ammoxidation activity mud that quantity is large, purity is higher, thereby have in actual applications certain restriction.
Summary of the invention
The apparatus and method that will provide a kind of fast enriching propagation and purifying to cultivate anaerobic ammonia oxidizing bacteria are provided.These apparatus and method can realize holding back adhering to of anaerobic ammonium oxidizing bacteria, enrichment and propagation.
The technical scheme that realizes the object of the invention is:
A kind of fast enriching propagation and purifying are cultivated the device of anaerobic ammonia oxidizing bacteria, by water inlet preheating system, slot type biological reaction tank and filtering basin three parts, are linked in sequence and are formed.
Described water inlet preheating system is comprised of preheating pond body, water inlet pipe, heating arrangements, temperature measuring mechanism, temperature control device and peristaltic pump, heating arrangements and temperature measuring mechanism are arranged in the body of preheating pond, peristaltic pump respectively with the helical heating pipe of heating arrangements and slot type biological reaction tank with pipeline communication.
Described slot type biological reaction tank is arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tihe and the foam type wrapper plate be located on the dignity of pond forms, and between cloth shape hanging type fibrous type filler, is provided with baffle plate.
Described filtering basin are arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tiji and the foam type wrapper plate be located on the dignity of pond forms, and between cloth shape hanging type fibrous type filler, are provided with baffle plate, and filtering basin are provided with water outlet.
Described slot type biological reaction tank is the rectangular parallelepiped great Chi that the degree of depth is 2-2.5m, and volume is 10-20m
3.
A kind of method that fast enriching propagation and purifying are cultivated anaerobic ammonia oxidizing bacteria, comprise the steps:
(1) arrange one by water inlet preheating system, slot type biological reaction tank and filtering basin three parts be linked in sequence the fast enriching propagation that forms and the device of purifying cultivation anaerobic ammonia oxidizing bacteria;
(2) place in advance a certain amount of aerobic activated sludge filtered through elutriation in the slot type biological reaction tank, and it is mixed with nutritive medium, make anaerobic ammonia oxidizing bacteria propagation;
(3) progressively pass into the nutritive medium through the heating of water inlet preheating system in the slot type biological reaction tank;
(4) by being arranged on the cloth shape hanging type fibrous type filler in slot type biological reaction tank and filtering basin, hold back anaerobic ammonia oxidizing bacteria, make its enrichment;
(5) after 60-90 days cultivate, on cloth shape hanging type fibrous type filler, cover with henna anaerobic ammonia oxidizing bacteria;
(6) cloth shape hanging type fibrous type filler is taken out, with useless nutrient solution wash-out, natural sedimentation, can obtain the Anammox denitrifying sludge of larger amt and higher degree.
The described nutritive medium trace element that to be ammonia and nitrite need by weight ratio and the microorganism growth of 1:1-1.3 is formulated, and total nitrogen content is 100-600mg/L.
After testing, the SS of Anammox denitrifying sludge is about 1000-6000mg/L, 50-500L, purity >=80%.
Advantage of the present invention is:
(1) technical measures that the present invention takes comprise volume and the degree of depth that increases breeder reactor, can hold the enrichment of anaerobic ammoxidation activity mud and the proliferate of larger amt;
(2) adopt the auxiliary traverse baffle formed of the strip multiporous fiber cloth filler dangled, for the apposition growth of anaerobic ammonium oxidizing bacteria provides carrier, thereby to generation time longer Anammox play the enriching and purifying effect of holding back, and be easy to adhere to the anaerobic ammonium oxidizing bacteria wash-out separate and collect after cultivate finishing;
(3), by baffling repeatedly, the nutrient solution water inlet entered can fully be contacted, for the growth of anaerobic ammonium oxidizing bacteria provides sufficient nutriment to guarantee to reach the purpose of fast breeding and purifying with thalline;
(4) darker pond body adopt internal recycle to stir and mode that the water surface covers plastic foamboard has reduced dissolving in of oxygen in the air, guaranteed the anaerobic state in the pond, but the also abundant mixing of assurance muddy water;
(5) effect of screening and enrichment propagation, the effect that can play wash-out, screen out other miscellaneous bacterias are played in the water inlet of the nutritive medium of artificial preparation to anaerobic ammonium oxidizing bacteria; Adopt the mode of water inlet pipe coiling and water-bath indirect heating both to maintain the temperature in reaction tank and can prevent the impact that direct heating carries out bacterial growth, nutritive medium, the mode that adopts water outlet to hold back has again further increased holding back of anaerobic ammonium oxidizing bacteria and taking full advantage of nutritive medium, thereby these apparatus and method can realize the target of fast enriching propagation and purifying anaerobic oxidation bacterium, thereby provide sufficient seed sludge for starting the Anammox denitrification reactor, realize starting fast the purpose that the oxidation oxidation reactor removes ammonia nitrogen and nitrite nitrogen in waste water.
The accompanying drawing explanation
Fig. 1 is the structural representation that fast enriching propagation of the present invention and purifying are cultivated the device of anaerobic ammonia oxidizing bacteria.
In figure: 1. spillway 11. water outlet 12. pipelines of water inlet pipe 2. heating arrangements 3. temperature control device 4. temperature measuring mechanism 5. peristaltic pump 6. foam type wrapper plate 7. baffle plate 8. cloth shape hanging type fibrous type filler 9. water outlet overflow port 10. filtering basin.
Embodiment
Embodiment 1:
With reference to Fig. 1, a kind of fast enriching propagation and purifying are cultivated the device of anaerobic ammonia oxidizing bacteria, by water inlet preheating system, slot type biological reaction tank and filtering basin three parts, are linked in sequence and are formed.
The water inlet preheating system is comprised of preheating pond body, water inlet pipe 1, heating arrangements 2, temperature measuring mechanism 4, temperature control device 3 and peristaltic pump 5, heating arrangements 2 and temperature measuring mechanism 4 are arranged in the body of preheating pond, and peristaltic pump 5 is communicated with pipeline 12 with helical heating pipe and the slot type biological reaction tank of heating arrangements 2 respectively.
The slot type biological reaction tank is arranged on the cloth shape hanging type fibrous type filler 8 more than two groups in the body of pond by Chi Tihe and the foam type wrapper plate 6 be located on the dignity of pond forms, be provided with baffle plate 7 between cloth shape hanging type fibrous type filler 8, be provided with water outlet overflow port 9 on the slot type biological reaction tank.
Filtering basin are arranged on the cloth shape hanging type fibrous type filler 8 more than two groups in the body of pond by Chi Tiji and the foam type wrapper plate 6 be located on the dignity of pond forms, be provided with baffle plate 7 between cloth shape hanging type fibrous type filler 8, filtering basin are provided with spillway 10 and water outlet 11.
The slot type biological reaction tank is the rectangular parallelepiped great Chi that the degree of depth is 2-2.5m, and volume is 10-20m
3.
It is 10 m that the nutrient solution of heating is pumped into to volume
3reaction tank in, place the cloth shape porous fiber packing frame of easy-offtaking, the excess sludge of aerobic sewage work that adds 50L is as the initial source of anaerobic ammonium oxidizing bacteria and start the internal recycle agitator, connect the nutrient solution water inlet system, the nutrient solution prepared is squeezed into to reaction tank by the flow velocity of 2L/ minute with pump, every day 2 times, within one time 30 minutes, start the internal recycle agitator, cultivate 60-90 days, while on fibrous packing, covering with dirt settling russet, fibrous packing is taken out, the wash-out dirt settling, quiescent setting, the skim supernatant liquor, stay the sorrel slurry of bottom, be the Anammox denitrifying activated sludge of high purity (content of anaerobic ammonia oxidizing bacteria>=80%).The impact of the cloth shape porous fiber packing quantity that once obtainable Anammox amount of sludge is laid is generally the about 100L of bacterium liquid that SS is 2000mg/L.
Embodiment 2:
To squeeze into volume through pre-warmed nutrient solution is 10m
3reaction tank in, place the prefabricated traverse baffle formed by fibrous packing in pond, add initial source the startup internal recycle agitator of the sediment of pond of 100L as anaerobic ammonium oxidizing bacteria, connect the nutrient solution water inlet system, the nutrient solution prepared is squeezed into to reaction tank by the flow velocity of 3L/ minute with pump, started 1 time every day every 6 hours, within one time 20 minutes, starting internal recycle agitator stirring water inlet fully mixes it with the mud of bottom deposition, cultivate 60-70 days, while on fibrous packing, covering with dirt settling russet, fibrous packing is taken out, the dirt settling of wash-out on it, quiescent setting, the skim supernatant liquor, stay the sorrel slurry of bottom, be the Anammox denitrifying activated sludge of high purity (content of anaerobic ammonia oxidizing bacteria>=80%).The cloth shape porous fiber packing number affects that once obtainable Anammox amount of sludge is laid is generally the about 150L of bacterium liquid that SS is 2000mg/L.All the other are with embodiment 1.
Embodiment 3:
To squeeze into volume through pre-warmed nutrient solution is 10m
3reaction tank in, settle the prefabricated traverse baffle formed by fibrous packing in pond, add taking from the anaerobic treatment granule sludge as the initial source of anaerobic ammonium oxidizing bacteria and starting the internal recycle agitator of 200L, connect the nutrient solution water inlet system, the nutrient solution prepared is squeezed into to the bottom of reaction tank one end by the flow velocity of 5L/ minute with peristaltic pump, started 1 time every day every 4 hours, within one time 10 minutes, starting the internal recycle agitator fully mixes water inlet and bottom settled sludge, cultivate 60-80 days, while on fibrous packing, covering with dirt settling russet, fibrous packing is taken out, the dirt settling of wash-out on it, quiescent setting, the skim supernatant liquor, stay the sorrel slurry of bottom, be the Anammox denitrifying activated sludge of high purity (content of anaerobic ammonia oxidizing bacteria>=80%).The impact of the cloth shape porous fiber packing quantity that once obtainable Anammox amount of sludge is slightly laid is generally the about 300L of bacterium liquid that SS is 2000mg/L.All the other are with embodiment 1.
Claims (3)
1. fast enriching propagation and purifying are cultivated the device of anaerobic ammonia oxidizing bacteria, it is characterized in that: this device is linked in sequence and is formed by intake preheating system, slot type biological reaction tank and filtering basin three parts;
Described water inlet preheating system is comprised of preheating pond body, water inlet pipe, heating arrangements, temperature measuring mechanism, temperature control device and peristaltic pump, heating arrangements and temperature measuring mechanism are arranged in the body of preheating pond, and peristaltic pump is connected with pipeline with the slot type biological reaction tank with the helical heating pipe of heating arrangements respectively;
Described slot type biological reaction tank is arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tihe and the foam type wrapper plate be located on the dignity of pond forms, and between cloth shape hanging type fibrous type filler, is provided with baffle plate;
Described filtering basin are arranged on the cloth shape hanging type fibrous type filler more than two groups in the body of pond by Chi Tiji and the foam type wrapper plate be located on the dignity of pond forms, and between cloth shape hanging type fibrous type filler, are provided with baffle plate, and filtering basin are provided with water outlet.
2. the method for fast enriching propagation and purifying cultivation anaerobic ammonia oxidizing bacteria, is characterized in that: comprise the steps:
(1) arrange one by water inlet preheating system, slot type biological reaction tank and filtering basin three parts be linked in sequence the fast enriching propagation that forms and the device of purifying cultivation anaerobic ammonia oxidizing bacteria;
(2) place in advance a certain amount of aerobic activated sludge filtered through elutriation in the slot type biological reaction tank, and it is mixed with nutritive medium, make anaerobic ammonia oxidizing bacteria propagation;
(3) progressively pass into the nutritive medium through the heating of water inlet preheating system in the slot type biological reaction tank;
(4) by being arranged on the cloth shape hanging type fibrous type filler in slot type biological reaction tank and filtering basin, hold back anaerobic ammonia oxidizing bacteria, make its enrichment;
(5) after 60-90 days cultivate, on cloth shape hanging type fibrous type filler, cover with henna anaerobic ammonia oxidizing bacteria;
(6) cloth shape hanging type fibrous type filler is taken out, with useless nutrient solution wash-out, natural sedimentation, can obtain the Anammox denitrifying sludge of larger amt and higher degree.
3. method according to claim 2 is characterized in that: it is formulated by the trace element of the weight ratio of 1:1-1.3 and microorganism growth needs that described nutritive medium is ammonia and nitrite, and total nitrogen content is 100-600mg/L.
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Cited By (4)
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| CN103288223A (en) * | 2013-06-09 | 2013-09-11 | 北京工业大学 | Ammonia oxidizing bacterium enriching device and method suitable for purifying micro-polluted water and application of ammonia oxidizing bacterium enriching device and method |
| CN103663685A (en) * | 2013-11-12 | 2014-03-26 | 桂林电子科技大学 | Multi-bed biomembrane reactor for culture of anaerobic ammoxidation bacteria |
| CN104512962A (en) * | 2013-09-30 | 2015-04-15 | 北京师范大学 | Method for enhancing anaerobic ammoxidation effect of deposit by using water treatment plant waste mud |
| CN111547866A (en) * | 2020-06-11 | 2020-08-18 | 北京工业大学 | Device and method for culturing and enriching anaerobic ammonium oxidation sludge |
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