CN103083571A - Method for joint preparation of reed rhizome polysaccharide and ferulic acid - Google Patents
Method for joint preparation of reed rhizome polysaccharide and ferulic acid Download PDFInfo
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- CN103083571A CN103083571A CN2012105494812A CN201210549481A CN103083571A CN 103083571 A CN103083571 A CN 103083571A CN 2012105494812 A CN2012105494812 A CN 2012105494812A CN 201210549481 A CN201210549481 A CN 201210549481A CN 103083571 A CN103083571 A CN 103083571A
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- rhizoma phragmitis
- ferulic acid
- reed rhizome
- polysaccharide
- microwave
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Abstract
The invention discloses a method for a joint preparation of reed rhizome polysaccharide and ferulic acid, and belongs to the technical field of extracting a natural product. The method comprises the following steps of: extracting pre-processed reed rhizome through an ultrasonic-microwave synergic effect by taking water as an extracting agent, condensing the extract liquid, depositing by alcohol, filtering, and drying and grinding a filter cake to obtain reed rhizome polysaccharide; recovering the ethanol from the extract liquid until no alcohol smell, and separating through a macroporous absorption resin to obtain reed rhizome and ferulic acid products. The method disclosed by the invention is simple in process route; and the method, which prepares two valuable bioactive substances: the reed rhizome polysaccharide and the ferulic acid, through a same batch of raw materials, achieve full utilization of reed rhizome resource, so that production cost is reduced, and economic benefit is improved.
Description
Technical field
The present invention relates to a kind of method for preparing active polysaccharide and ferulic acid of uniting take Rhizoma Phragmitis as raw material, belong to the natural product extracting technique field.
Background technology
Rhizoma Phragmitis is the rhizome of grass phragmites communis.Modern medicine thinks that Rhizoma Phragmitis has clearing away heat and promoting production of body fluid, relieving restlessness, preventing or arresting vomiting, the effect of diuresis, in medical Rhizoma Phragmitis treatment calentura excessive thirst, gastric heat the vomit sound of vomiting, cough due to lung-heat, lung abscess vomiting pus, the puckery pain of pyretic stranguria.
Contain various bioactivators in Rhizoma Phragmitis.Active polysaccharide in Rhizoma Phragmitis is made of glucose, xylose, arabinose residue; and contain the side chain of β (1 → 3) glycosidic bond; arabinose in molecule is levulose; make the Rhizoma Phragmitis polysaccharide have stronger non-oxidizability, can remove biological interior free yl, protect hepatocyte, improve liver function, reduce liver fatization degree and suppress hepatic fibrosis.Contain multiple phenolic acid in Rhizoma Phragmitis; as: ferulic acid, gentisic acid, caffeic acid, syringic aldehyde, coniferyl aldehyde, vanillic acid, 2; 5-dimethoxy-p-benzoquinone, p-hydroxybenzaldehyde, p-coumaric acid etc.; wherein ferulic acid is one of main effective ingredient, has the effects such as the not oxidated and anticoagulant of free radical resisting, protecting film, thrombosis.
Polysaccharide is the polarity macromolecular compound, can utilize polysaccharide to be dissolved in hot water or acid, alkali, saline solution and is insoluble to the characteristic of alcohol, ether, acetone and other organic solvent, adopts water, diluted alkaline or saline solution etc. to extract for extracting medium.Ferulic acid is dissolved in hot water, ethanol and ethyl acetate, and water commonly used, methanol and ethanol are made the extraction solvent.
At present, some researcheres have been carried out the research of extracting functional components in Rhizoma Phragmitis, as: decoction and alcohol sedimentation technique extracts Rhizoma Phragmitis polysaccharide (Anhui Chinese Medicine College journal, 2002,1:51～52), Enzymatic Extraction Rhizoma Phragmitis polysaccharide (food industry science and technology, 2009,4:156～159), ultrasound wave assisted extraction Rhizoma Phragmitis polysaccharide (Agriculture of Anhui science, 2011,33:20407～20410), ethanol refluxing process extracts in Rhizoma Phragmitis ferulic acid (Chizhou City's Journal of Teachers College, 2007,3:51～52) etc.These methods are only extracted single functional components from Rhizoma Phragmitis, raw material availability is low.
Summary of the invention
The object of the present invention is to provide a kind of method for preparing Rhizoma Phragmitis polysaccharide and ferulic acid of uniting, utilize with a collection of raw material production Rhizoma Phragmitis polysaccharide and ferulic acid product, reduce production costs, alleviate the wasting of resources that causes when producing single product.Adopt ultrasonic-microwave synergistic extraction technology in method of the present invention, utilize the cavitation of ultrasonic vibration and the high energy effect of microwave, destroy the Rhizoma Phragmitis cell wall, be conducive to the leaching of functional components in Rhizoma Phragmitis.
A kind of method for preparing Rhizoma Phragmitis polysaccharide and ferulic acid of uniting of the present invention comprises the following steps:
(1) pretreatment of raw material: after new fresh Rhizoma Phragmitis is cleaned, shreds, dry by the fire 12h in 70 ℃ of air dry ovens, pulverize to get the Rhizoma Phragmitis powder;
(2) ultrasonic-microwave synergistic extraction: the Rhizoma Phragmitis powder of getting step (1) gained, distilled water immersion 20min with 10～40 times of its quality, then move in ultrasonic-microwave chemical combined reactor, extract 8～40min under supersonic frequency 25kHz, ultrasonic power 50～300w, microwave frequency 2450MHz, microwave power 100～600w condition;
(3) preparation Rhizoma Phragmitis polysaccharide and ferulic acid: after ultrasonic-microwave synergistic extraction is completed, filter, get filtrate and through vacuum concentration to 1/6～1/10 of former filtrate volume, then with 95% ethanol precipitation of 4 times of volumes, filtration gets filter cake A and liquor B; Filter cake A gets the Rhizoma Phragmitis crude polysaccharides through vacuum drying, pulverizing; Liquor B is separated with macroporous adsorbent resin after without the alcohol flavor through decompression recycling ethanol, gets Rhizoma Phragmitis ferulic acid product.
Great advantage of the present invention is to utilize to prepare Rhizoma Phragmitis polysaccharide and two kinds of value products of ferulic acid with a collection of raw material, takes full advantage of resource, the wasting of resources that has caused when having alleviated the preparation single product.
Secondly, adopt ultrasonic-microwave synergistic extraction technology, utilize the cavitation of ultrasonic vibration and the high energy effect of microwave, destroy the Rhizoma Phragmitis cell wall, be conducive to the leaching of functional components in Rhizoma Phragmitis, improved the extraction ratio of Rhizoma Phragmitis polysaccharide and ferulic acid, shortened extraction time.
The specific embodiment
Embodiment 1
After new fresh Rhizoma Phragmitis is cleaned, shreds, dry by the fire 12h in 70 ℃ of air dry ovens, pulverize to get the Rhizoma Phragmitis powder; Take the Rhizoma Phragmitis powder, with the distilled water immersion 20min of 25 times of its quality, then move in ultrasonic-microwave chemical combined reactor, extract 17min under supersonic frequency 25kHz, ultrasonic power 100w, microwave frequency 2450MHz, microwave power 300w condition; After extraction is completed, filter, get filtrate and be concentrated into 1/8 of former filtrate volume under vacuum 0.08Mpa, temperature 60 C condition, then precipitate with 95% ethanol of 4 times of volumes, filter, get filter cake A and liquor B; Filter cake A gets the Rhizoma Phragmitis crude polysaccharides through vacuum drying, pulverizing; Liquor B is after decompression recycling ethanol to nothing alcohol is distinguished the flavor of, add on the D101 type macroporous resin column of anticipating, first wash away water-solubility impurity in liquor B with the distilled water macroporous resin column of flowing through, be that 45% ethanol water stream is washed macroporous resin column and got eluent with volume fraction again, eluent to doing, gets Rhizoma Phragmitis ferulic acid product through reduction vaporization.
Claims (1)
1. unite the method for preparing Rhizoma Phragmitis polysaccharide and ferulic acid for one kind, comprise following processing step:
(1) pretreatment of raw material: after new fresh Rhizoma Phragmitis is cleaned, shreds, dry by the fire 12h in 70 ℃ of air dry ovens, pulverize to get the Rhizoma Phragmitis powder;
(2) ultrasonic-microwave synergistic extraction: the Rhizoma Phragmitis powder of getting step (1) gained, distilled water immersion 20min with 10～40 times of its quality, then move in ultrasonic-microwave chemical combined reactor, extract 8～40min under supersonic frequency 25kHz, ultrasonic power 50～300w, microwave frequency 2450MHz, microwave power 100～600w condition;
(3) preparation Rhizoma Phragmitis polysaccharide and ferulic acid: after ultrasonic-microwave synergistic extraction is completed, filter, get filtrate and through vacuum concentration to 1/6～1/10 of former filtrate volume, then with 95% ethanol precipitation of 4 times of volumes, filtration gets filter cake A and liquor B; Filter cake A gets the Rhizoma Phragmitis crude polysaccharides through vacuum drying, pulverizing; Liquor B is separated with macroporous adsorbent resin after without the alcohol flavor through decompression recycling ethanol, gets Rhizoma Phragmitis ferulic acid product.
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| CN2012105494812A CN103083571A (en) | 2012-12-18 | 2012-12-18 | Method for joint preparation of reed rhizome polysaccharide and ferulic acid |
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| CN2012105494812A CN103083571A (en) | 2012-12-18 | 2012-12-18 | Method for joint preparation of reed rhizome polysaccharide and ferulic acid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400744A (en) * | 2018-11-21 | 2019-03-01 | 湖南中医药大学 | A kind of oxidation resistant southern reed bamboo shoot polyoses producing method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102432691A (en) * | 2011-12-12 | 2012-05-02 | 辽宁仙鹤矿泉水有限公司 | Method for extracting polysaccharides from reed rhizome |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432691A (en) * | 2011-12-12 | 2012-05-02 | 辽宁仙鹤矿泉水有限公司 | Method for extracting polysaccharides from reed rhizome |
Non-Patent Citations (5)
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| 姚以才等: "芦根多糖的超声辅助提取及其抗菌活性", 《食品科学》 * |
| 晁若瑜等: "芦根多糖的分离纯化和体外抗肿瘤研究", 《食品工业科技》 * |
| 汪美芳: "芦根中阿魏酸的提取、分离和检测", 《池州师专学报》 * |
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| 赵超等: "物理场强化植物多糖提取研究进展", 《现代化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400744A (en) * | 2018-11-21 | 2019-03-01 | 湖南中医药大学 | A kind of oxidation resistant southern reed bamboo shoot polyoses producing method |
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Application publication date: 20130508 |