CN103083136A - Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material - Google Patents
Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material Download PDFInfo
- Publication number
- CN103083136A CN103083136A CN2012105758420A CN201210575842A CN103083136A CN 103083136 A CN103083136 A CN 103083136A CN 2012105758420 A CN2012105758420 A CN 2012105758420A CN 201210575842 A CN201210575842 A CN 201210575842A CN 103083136 A CN103083136 A CN 103083136A
- Authority
- CN
- China
- Prior art keywords
- bacterial cellulose
- liquid
- sandwich structure
- air pressure
- fermentation culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relate to bacterial cellulose liquid absorbing material of a sandwich structure and a preparation method of the bacterial cellulose liquid absorbing material. The bacterial cellulose liquid absorbing material of the sandwich structure is prepared by the steps such as culture expansion cultivation, stationary culture and postprocessing, and composed of three bacterial cellulose membranes which are tightly combined, the three bacterial cellulose membranes comprise an upper ultra absorbing layer, a lower ultra absorbing layer and a liquid storing layer in the middle. Tight combination refers to that cellulose microfiber of the upper ultra absorbing layer and the lower ultra absorbing layer are combined with cellulose microfiber of the liquid storing layer by intermolecular and intermolecular hydrogen bonding in bi-1, 4-glucan chain to form a molecular layer, layers are combined by intermolecular and intermolecular hydrogen bonding, obvious physical layering does not exist, and structural continuity is good. Prepared liquid absorbing material has good liquid absorbing performance, water binding capacity, biocompatibility and mechanical performance. The bacterial cellulose liquid absorbing material of the sandwich structure is simple and quick in preparation process, green and environmental-friendly, short in cultivation period, low in cost, and applied to the fields such as medicine, personal care and daily-used mechanical industry.
Description
Technical field
The present invention relates to Bacterial cellulose liquid-absorbent material of a kind of liquid-absorbent material and preparation method thereof, particularly a kind of sandwich structure and preparation method thereof.
Background technology
At present, the liquid-absorbent material has a wide range of applications in a lot of fields.As: the hemostatic material in bio-medical field, can control blood oozing from the wound surface, guarantee that surgical field of view is clear, improve operation efficient; The dressing of absorbing wound exudate can extend the service time of dressing on exudative wound, reduces treatment cost, effectively shortens the healing time of wound; The liquid-absorbent material that is used for personal nursing can be applied to diaper, tempers trousers, sanitary towel, incontinence be with underwear, binder etc., and cleaning, comfortable Personal hygiene environment are provided.
Liquid-absorbent material requirements material has the ability of good absorption liquid, usually uses hydrophilic macromolecular material preferably.And along with the progress of society, the raising of people's quality of the life is had higher requirement to the liquid-absorbent material.Require the liquid-absorbent material to have better liquid-absorbent, biocompatibility, comfortableness and mechanical property.
Bacterial cellulose (Bacterial Cellulose also claims micro organism cellulose) is a kind of nano-fiber material.Its with bacterial cell inside as the biosynthesis reactor, glucose micromolecule allosteric process through series of complex under enzyme catalysis is finally passed through β-1, the 4-glycosidic bond is extruded in conjunction with forming the catalytic site that β-Isosorbide-5-Nitrae-the Fructus Vitis viniferae sugar chain is pne cell by antibacterial.β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain is and intermolecular hydrogen bonding effect interior by molecule each other, progressively, hierarchically forms lipopolysaccharide layer, tactoid, cellulose fento and the final cellulose that forms.This a series of extracellular (Extracellular) forming process is called as " cellulosic self assembly ".The process of this unique microorganism participation has given Bacterial cellulose good physicochemical property just
Bacteria cellulose material has ultra-fine tridimensional network; Good moisture absorption, moisturizing and permeability; The retentiveness of superelevation and wet strength; High-tensile and elastic modelling quantity etc.Studies show that in a large number bacteria cellulose material has in good body, biocompatibility in vitro, add shape Modulatory character that it is excellent and shape maintains make its in construct, the vitro tissue engineering scaffold material has advantageous advantage.The industries such as food, medicine, weaving, papermaking, chemical industry, oil recovery, ore dressing have been applied at present.Along with updating of fermentation technology, output and the production capacity of Bacterial cellulose are improved gradually, and cost reduces gradually.Liquid-absorbent material with the Bacterial cellulose preparation can be widely used in each fields such as medicine, personal nursing, daily-use chemical industry.
Summary of the invention
The Bacterial cellulose liquid-absorbent material of a kind of sandwich structure of the present invention, be to close closely by three-layered node that bacteria cellulose film consists of, described three-layered node closes bacteria cellulose film closely and comprises super layer and the lower super reserve liquid layer of inhaling layer and mediating inhaled; Described combination refers to that closely the cellulose microfibril of described super suction layer and the cellulose microfibril of described reserve liquid layer pass through β-1, be combined with intermolecular hydrogen bonding in molecule in 4-Fructus Vitis viniferae sugar chain, form molecular layer, also be combined with intermolecular hydrogen bonding by in molecule between layers, without obvious physical layering; The elementary cell that forms Bacterial cellulose is not single β-1,4-Fructus Vitis viniferae sugar chain, but pre-microfibril (premicrofibril), it is comprised of β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain, every 9 β-1,4-Fructus Vitis viniferae sugar chain is parallel to each other, and by being combined with intermolecular hydrogen bonding in molecule, is left hand triple helical shape, be the ultimate unit that forms microfibril (microfibril), diameter is 1.5nm.Microfibril (microfibril) diameter is 3.5nm, fento be combined with intermolecular hydrogen bonding in by molecule between fento, β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain is parallel arrangement, forms cellulose I type crystalline texture.
The wherein said super content of cellulose of inhaling in layer and lower super suction layer is 0.7 * 10
-2～1.0 * 10
-2G/cm
3, the content of cellulose in described reserve liquid layer is 0.2 * 10
-2～0.5 * 10
-2G/cm
3
As preferred technical scheme:
The Bacterial cellulose liquid-absorbent material of a kind of sandwich structure as above, described bacteria cellulose film be by strain under standing condition of culture, the consumption sugar source is with synthetic β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain in cell and extrude cells in vitro.Article 3～4, β-1,4-Fructus Vitis viniferae sugar chain is by forming the lipopolysaccharide layer with the intermolecular hydrogen bonding effect in molecule, 4～5 lipopolysaccharide layers are by forming diameter at the tactoid of 1.5nm left and right with intermolecular hydrogen bonding in molecule, 3～5 tactoids are by forming diameter at the cellulose microfibril of 3.5nm left and right with intermolecular hydrogen bonding in molecule, many microfibrils are by forming cellulose tow with the intermolecular hydrogen bonding effect in molecule, and many tow are by forming the cellulose silk ribbon with the intermolecular hydrogen bonding effect in molecule.Bacterium cell can not affect cellulose silk ribbon fracture at the surperficial disordered motion of culture fluid even division occurs cell yet.Be interweaved by the cellulose microfibril that forms with intermolecular hydrogen bonding in molecule by β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain, pass through each other to interact with intermolecular hydrogen bonding in molecule, finally form similar and bacteria cellulose film nonwoven fabric construct at liquid level.
The thickness of the Bacterial cellulose liquid-absorbent material of sandwich structure as above is 9～16mm, and the wherein said super thickness of inhaling layer and lower super suction layer is 3～4mm, and the thickness of described reserve liquid layer is 3～8mm.
The present invention also provides a kind of Bacterial cellulose liquid-absorbent material preparation method of sandwich structure, comprises the following steps:
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 2～5, peptone 0.05 ~ 0.5, yeast extract 0.05 ~ 0.5, citric acid 0.05 ~ 0.5, sodium hydrogen phosphate 0.05 ~ 0.5, potassium dihydrogen phosphate 0.05 ~ 0.5, surplus is water;
The pH of fermentation culture is 4.0～6.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
5～2 * 10
7Individual/ml.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 28～32 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent in the upper and lower part of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1～2 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2～3 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, keeps simultaneously the carrier of oxygen volume concentrations in 10～1% scopes; Reach 0.5～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 3～6 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 1～2 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improves simultaneously oxygen concentration to 50%; Until the Bacterial cellulose film thickness rises to 3～4mm;
Reserve liquid layer formation stages 1～3 day reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10～15% scope; Until the Bacterial cellulose film thickness rises to 6～8mm;
Upper super suction layer formation stages 1～2 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improve simultaneously oxygen concentration to 50%, until the Bacterial cellulose film thickness is when rising to 9～12mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1～10wt%, boil and kept 2～10 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again or part setting-out are processed, and are the Bacterial cellulose liquid-absorbent material of sandwich structure.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, after described high pressure steam sterilization, ultraviolet irradiation refers to that above-mentioned fermentation culture is placed in high-pressure sterilizing pot 121 ℃ of sterilization treatment to be taken out after 30 minutes and be placed in that under uviol lamp, irradiation is cooled to room temperature.
Strain as above refers to can the cellulosic microorganism of biosynthesis, comprising: acetobacter xylinum, produce one or more in acetobacter, acetify bacillus, Acetobacter pasteurianus, glucose bacillus, Agrobacterium, root nodule bacteria, sarcina, Pseudomonas cepacia, Pseudomonas cocovenenans or campylobacter jejuni.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, described logical pure oxygen refer to the speed of medical oxygen with 1L/min is passed in above-mentioned culture fluid, and keep 30 minutes; Described inoculation refers to hook up with the inoculating loop after sterilization and is stored in right amount 4 ℃ of strains in lower test tube, and is transferred in above-mentioned fermentation culture; Described spreading cultivation refers to the fermentation culture after the access strain was cultivated 8～24 hours in 28～32 ℃ of lower shaking tables.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, described moment pressurization, continuous pressure and three kinds of forms of interim pressurization of being pressurised into.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, described moment pressurization refers to the internal tank air pressure was promoted in 1.1～1.5 barometric pressure range in 30 minutes, and maximum is no more than 1.5 atmospheric pressure; Described continuous pressure per hour refers to that in container charged pressure percent is 0.1389～2.083% air, and radix is 1 normal atmosphere, until the container inner air pressure is no longer to increase pressure in 1.1～1.5 normal atmosphere scopes the time; Described interim pressurization refer to every 12 hours in the container charged pressure percent be 1.67～25% air, radix is 1 normal atmosphere equally, until the container inner air pressure is no longer to increase pressure in 1.1～1.5 normal atmosphere scopes the time.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, described raising oxygen concentration are moment oxygen supplement, increase continuously and three kinds of modes of interim increase;
Described moment oxygen supplement refers to: in 30 minutes, oxygen partial pressure is promoted to 50%;
Described increasing continuously refers to: oxygen concentration per hour increases by 48.61～166.67%, and radix is 10～15%, until oxygen concentration no longer increases when being 50%;
Described interim increasing refers to: oxygen concentration increased by 583.3～2000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%.
The Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as above, the Bacterial cellulose liquid-absorbent material preparation method of described a kind of sandwich structure, it is characterized in that, described reduction air pressure refers to: the air pressure that contacted with the bacteria cellulose film upper surface in container in 30 minutes is reduced to 1 normal atmosphere; Described reduction oxygen concentration refers to: in 30 minutes with container in oxygen concentration be reduced in 10～15% scopes.
Bacterial cellulose possesses its unique physics, chemistry and engineering properties as a kind of novel natural water gel: ultra-fine network structure; High-tensile and elastic modelling quantity; High-hydrophilic, good ventilative, suction, water permeability, and outstanding retentiveness and high wet strength are arranged.The microfibril bundle diameter of Bacterial cellulose is 3～4nm, and is 70～80nm by the cellosilk bandwidth that microfiber bundle connects into, and length is 1～9 μ m, is the thinnest present natural fiber.
Bacterial cellulose liquid-absorbent material of a kind of sandwich structure of this patent invention and preparation method thereof, in incubation, by controlling and the contacted air pressure of Bacterial cellulose thin film and oxygen concentration, obtain a kind of by upper super layer, the lower super reserve liquid layer of inhaling layer and mediating inhaled, in conjunction with the Bacterial cellulose liquid-absorbent material of the sandwich structure that closely consists of.This liquid-absorbent material has good liquid-absorbent, retentiveness, comfortableness, biocompatibility and mechanical property, the preparation process Simple fast, environmental protection, cultivation cycle is short, with low cost, can be applied to each fields such as medicine, personal nursing, daily-use chemical industry.
Beneficial effect:
With prior art first than, the invention has the beneficial effects as follows:
(1) Bacterial cellulose liquid-absorbent material of a kind of sandwich structure and preparation method thereof, preparation process is continuous, super inhale layer and is combined with intermolecular hydrogen bonding in by molecule by the nanometer microfibril between reserve liquid layer.Make cellulose microfibril that β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain consists of by be combined with intermolecular hydrogen bonding in molecule and the process of crystalline forming in, spontaneous, form fine and close structure to the gradual change of loosening in an orderly manner.Combination degree is darker, not only is confined to material surface.
(2) a kind of Bacterial cellulose liquid-absorbent material of sandwich structure, without obvious physical layering, structural continuity is good; Surpassing the suction layer makes material possess good mechanical performance with the interior structure gradient variation that exists of reserve liquid layer.
(3) Bacterial cellulose liquid-absorbent material of a kind of sandwich structure and preparation method thereof, on the prior art basis, by increasing in incubation and the contacted air pressure of Bacterial cellulose thin film, make the inner aerobic of Bacterial cellulose thin film district area change; Simultaneously accurate controlled pressure does not make the Bacterial cellulose thin film sink.Improved only to rely in the prior art and improved the single technique that oxygen partial pressure is constructed the density structure, also solved the too high defective that causes the cellulose growth rate to slow down of oxygen partial pressure simultaneously.
(4) Bacterial cellulose liquid-absorbent material of a kind of sandwich structure and preparation method thereof, have good fluid absorbent and water retention property, by controlling reserve liquid layer and upper and lower super thickness and thickness proportion of inhaling layer in the liquid-absorbent material, can effectively regulate the maximum absorption, liquid absorption of liquid absorbent material, effective soak time etc.
(5) Bacterial cellulose liquid-absorbent material of a kind of sandwich structure and preparation method thereof, the preparation process Simple fast, environmental protection, cultivation cycle is short, and is with low cost, can be applied to each fields such as medicine, personal nursing, daily-use chemical industry.
The specific embodiment
Below in conjunction with the specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
The Bacterial cellulose liquid-absorbent material of a kind of sandwich structure of the present invention, be to close closely by three-layered node that bacteria cellulose film consists of, described three-layered node closes bacteria cellulose film closely and comprises super layer and the lower super reserve liquid layer of inhaling layer and mediating inhaled; Described combination refers to that closely the cellulose microfibril of described super suction layer and the cellulose microfibril of described reserve liquid layer pass through β-1, be combined with intermolecular hydrogen bonding in molecule in 4-Fructus Vitis viniferae sugar chain, form molecular layer, also be combined with intermolecular hydrogen bonding by in molecule between layers, without obvious physical layering; The elementary cell that forms Bacterial cellulose is not single β-1,4-Fructus Vitis viniferae sugar chain, but pre-microfibril (premicrofibril), it is comprised of β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain, every 9 β-1,4-Fructus Vitis viniferae sugar chain is parallel to each other, and by being combined with intermolecular hydrogen bonding in molecule, is left hand triple helical shape, be the ultimate unit that forms microfibril (microfibril), diameter is 1.5nm.Microfibril (microfibril) diameter is 3.5nm, fento be combined with intermolecular hydrogen bonding in by molecule between fento, β-Isosorbide-5-Nitrae-Fructus Vitis viniferae sugar chain is parallel arrangement, forms cellulose I type crystalline texture.
The wherein said super layer of inhaling is 0.7 * 10 with the lower super content of cellulose of inhaling in layer
-2～1.0 * 10
-2G/cm
3, the content of cellulose in described reserve liquid layer is 0.2 * 10
-2～0.5 * 10
-2G/cm
3
Described bacteria cellulose film is by strain consumption sugar source, and eccrine fiber element microfibril is by being combined formation with intermolecular hydrogen bonding in molecule.
The thickness of the Bacterial cellulose liquid-absorbent material of described sandwich structure is 9～16mm, and the wherein said super thickness of inhaling layer and lower super suction layer is 3～4mm, and the thickness of described reserve liquid layer is 3～8mm.
Embodiment 1
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 2, and peptone 0.05, yeast extract 0.05, citric acid 0.01, sodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, surplus is water;
The pH of fermentation culture is 4.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
5
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 28 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 3 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 1 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improves simultaneously oxygen concentration to 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 1 day reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10～15% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 1 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improve simultaneously in oxygen concentration to 50% scope, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 10 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again is the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 2
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 6.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 32 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 2 days Bacterial cellulose growth inducing phases: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 3 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 15% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.5 normal atmosphere scopes, improves simultaneously oxygen concentration to 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 15% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.5 normal atmosphere scopes, improve simultaneously in oxygen concentration to 50% scope, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 10wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again is the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 3
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 3, and peptone 0.3, yeast extract 0.3, citric acid 0.05, sodium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.05, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 12.5% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 6 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 2 days, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.3 normal atmosphere scopes, improves simultaneously oxygen concentration to 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 12.5% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 2 days, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.3 normal atmosphere scopes, improve simultaneously in oxygen concentration to 50% scope, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 2wt%, boil and kept 4 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again is the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 4
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, the moment pressurization, make in 30 minutes with the contacted air pressure of Bacterial cellulose upper surface and be promoted to 1.1 normal atmospheres, moment oxygen supplement simultaneously makes in 30 minutes with the contacted oxygen concentration of Bacterial cellulose upper surface and is increased to 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10～15% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, the moment pressurization, make in 30 minutes with the contacted air pressure of Bacterial cellulose upper surface and be promoted to 1.1 normal atmospheres, moment oxygen supplement simultaneously makes in 30 minutes with the contacted oxygen concentration of Bacterial cellulose upper surface and is increased to 50%; Until the Bacterial cellulose film thickness when rising to 9～16mm, takes out it, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again is the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 5
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, the moment pressurization, make in 30 minutes with the contacted air pressure of Bacterial cellulose upper surface and be promoted to 1.5 normal atmospheres, moment oxygen supplement simultaneously makes in 30 minutes with the contacted oxygen concentration of Bacterial cellulose upper surface and is increased to 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10～15% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, the moment pressurization, make in 30 minutes with the contacted air pressure of Bacterial cellulose upper surface and be promoted to 1.5 normal atmospheres, moment oxygen supplement simultaneously makes in 30 minutes with the contacted oxygen concentration of Bacterial cellulose upper surface and is increased to 50%; Until the Bacterial cellulose film thickness when rising to 9～16mm, takes out it, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again is the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 6
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is at 2 * 107/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 0.1389% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.1 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 48.61%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 0.1389% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.1 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 48.61%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness when rising to 9～16mm, takes out it, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 80%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 7
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is at 2 * 107/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 2.083% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.5 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 166.67%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 2.083% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.5 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 166.67%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness when rising to 9～16mm, takes out it, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 70%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 8
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7Individual/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 1% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.3 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 100%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, continuous pressure, per hour charged pressure percent is 1% air in the container, radix is 1 normal atmosphere, until with the contacted air pressure of Bacterial cellulose upper surface be 1.3 normal atmospheres, increase continuously simultaneously oxygen concentration, per hour increase by 100%, radix is 10%, until oxygen concentration no longer increases when being 50%; Until the Bacterial cellulose film thickness when rising to 9～16mm, takes out it, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 60%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 9
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is at 2 * 107/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 1.67% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.1 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, simultaneously the interim oxygen concentration that increases, increased by 583.3% in every 12 hours, until oxygen concentration no longer increases when reaching 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 1.67% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.1 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, stage increases oxygen concentration simultaneously, increased by 583.3% in every 12 hours, until oxygen concentration no longer increases when reaching 50%, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 50%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 10
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7Individual/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 25% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.5 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, simultaneously the interim oxygen concentration that increases, increased by 2000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 25% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.5 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, stage increases oxygen concentration simultaneously, increased by 2000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 30%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Embodiment 11
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 5, and peptone 0.5, yeast extract 0.5, citric acid 0.1, sodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, surplus is water;
The pH of fermentation culture is 5.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
7Individual/mL.
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 30 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent of the levels of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10% scope; Reach 0.5mm～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 15% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.3 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, simultaneously the interim oxygen concentration that increases, increased by 1000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%; Until the Bacterial cellulose film thickness rises to 3mm～4mm;
Reserve liquid layer formation stages 2 days reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 3 days, interim pressurization, every 12 hours in the container charged pressure percent be 15% air, radix is 1 normal atmosphere, until no longer pressurize when being 1.3 normal atmospheres with the contacted air pressure of Bacterial cellulose upper surface, stage increases oxygen concentration simultaneously, increased by 1000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%, until the Bacterial cellulose film thickness is when rising to 9～16mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1wt%, boil and kept 2 hours, with pure water clean to pH be 7.0, Bacterial cellulose film portion setting-out after processing again process to solid content be 10%, be the Bacterial cellulose liquid-absorbent material of sandwich structure.
Claims (10)
1. the Bacterial cellulose liquid-absorbent material of a sandwich structure, it is characterized in that: the Bacterial cellulose liquid-absorbent material of described sandwich structure is to close closely by three-layered node that bacteria cellulose film consists of, and it is upper super layer and the lower super reserve liquid layer of inhaling layer and mediating inhaled that described three-layered node closes bacteria cellulose film closely; Described combination refers to that closely the described super layer of inhaling passes through β-1 with the cellulose microfibril of lower super suction layer and the cellulose microfibril of described reserve liquid layer, be combined with intermolecular hydrogen bonding in molecule in 4-Fructus Vitis viniferae sugar chain, form molecular layer, also be combined with intermolecular hydrogen bonding by in molecule between layers, without obvious physical layering;
The wherein said super content of cellulose of inhaling in layer and lower super suction layer is 0.7 * 10
-2～1.0 * 10
-2G/cm
3, the content of cellulose in described reserve liquid layer is 0.2 * 10
-2～0.5 * 10
-2G/cm
3
2. the Bacterial cellulose liquid-absorbent material of a kind of sandwich structure according to claim 1, is characterized in that, described bacteria cellulose film is by strain consumption sugar source, and eccrine fiber element microfibril is by being combined formation with intermolecular hydrogen bonding in molecule.
3. the Bacterial cellulose liquid-absorbent material of a kind of sandwich structure according to claim 1, it is characterized in that, described strain refers to can the cellulosic microorganism of biosynthesis, comprising: acetobacter xylinum, produce one or more in acetobacter, acetify bacillus, Acetobacter pasteurianus, glucose bacillus, Agrobacterium, root nodule bacteria, sarcina, Pseudomonas cepacia, Pseudomonas cocovenenans or campylobacter jejuni.
4. the Bacterial cellulose liquid-absorbent material of a kind of sandwich structure according to claim 1, it is characterized in that, the thickness of the Bacterial cellulose liquid-absorbent material of described sandwich structure is 9～16mm, the wherein said super thickness of inhaling layer and lower super suction layer is 3～4mm, and the thickness of described reserve liquid layer is 3～8mm.
5. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure as claimed in claim 1 is characterized in that comprising the following steps:
1) allotment of fermentation culture;
The fermentation culture fluid component is calculated in mass percent, and unit is wt%: glucose, fructose, sucrose or mannitol 2～5, peptone 0.05 ~ 0.5, yeast extract 0.05 ~ 0.5, citric acid 0.05 ~ 0.5, sodium hydrogen phosphate 0.05 ~ 0.5, potassium dihydrogen phosphate 0.05 ~ 0.5, surplus is water;
The pH of fermentation culture is 4.0～6.0;
Said components is mixed by ultraviolet irradiation after high pressure steam sterilization and is cooled to room temperature, and logical pure oxygen namely gets fermentation culture;
2) strain spreads cultivation;
Described fermentation culture is inoculated and spread cultivation; Degree spreads cultivation: the bacterium cell number is 2 * 10
5～2 * 10
7Individual/ml;
3) standing cultivation;
Bacterium liquid after spreading cultivation is transferred in the culture vessel that fermentation culture is housed, is positioned in constant incubator, 28～32 ℃ of standing cultivations;
By the whole air pressure of adjusted stepwise culture fluid top air and oxygen partial pressure realize the fine and close and high liquid-absorbent in the upper and lower part of Bacterial cellulose thin film, mid portion is loose and the sandwich structure of high retentiveness;
A. 1～2 day Bacterial cellulose growth inducing phase: controlling with the contacted air pressure of fermentation culture liquid level is 1 normal atmosphere, until antibacterial totally floats upper liquid level afterwards with the oxygen expenditure that dissolves in culture fluid, the translucent Bacterial cellulose thin film of one deck appears in liquid level;
B. the Bacterial cellulose fast growing period is 2～3 days: controlling with bacteria cellulose film upper surface air pressure is 1 normal atmosphere, regulates simultaneously the carrier of oxygen volume concentrations in 10～15% scopes; Reach 0.5～1.5mm to the Bacterial cellulose film thickness;
C. the steady trophophase of Bacterial cellulose is 3～8 days:
Steadily trophophase divides three phases successively:
Lower super suction layer formation stages 1～3 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improves simultaneously oxygen concentration to 50%; Until the Bacterial cellulose film thickness rises to 3～4mm;
Reserve liquid layer formation stages 1～2 day reduces air pressure and makes contacted air pressure to 1 normal atmosphere of bacteria cellulose film upper surface, reduces simultaneously in oxygen concentration to 10～15% scope; Until the Bacterial cellulose film thickness rises to 6～12mm;
Upper super suction layer formation stages 1～3 day, pressurization makes with the contacted air pressure of Bacterial cellulose upper surface in 1.1～1.5 normal atmosphere scopes, improve simultaneously oxygen concentration to 50%, until the Bacterial cellulose film thickness is when rising to 9～12mm, it is taken out, namely get the Bacterial cellulose thin film that possesses sandwich structure;
4) post processing;
After standing cultivation finishes, the above-mentioned Bacterial cellulose thin film that possesses the density structure is dipped in the sodium hydroxide solution that concentration is 1～10wt%, boil and kept 2～10 hours, with pure water clean to pH be 7.0, Bacterial cellulose thin film lyophilization after processing again or part setting-out are processed, and are the Bacterial cellulose liquid-absorbent material of sandwich structure.
6. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure according to claim 5, it is characterized in that, after described high pressure steam sterilization, ultraviolet irradiation refers to that described fermentation culture is placed in high-pressure sterilizing pot 121 ℃ of sterilization treatment to be taken out after 30 minutes and be placed in that under uviol lamp, irradiation is cooled to room temperature.
7. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure according to claim 5, is characterized in that, described logical pure oxygen refers to the speed of medical oxygen with 1L/min is passed in above-mentioned culture fluid, and keeps 30 minutes; Described inoculation refers to hook up with the inoculating loop after sterilization and is stored in right amount 4 ℃ of strains in lower test tube, and is transferred in above-mentioned fermentation culture; Described spreading cultivation refers to the fermentation culture after the access strain was cultivated 8～24 hours in 28～32 ℃ of lower shaking tables.
8. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure according to claim 5, is characterized in that, described moment pressurization, continuous pressure and three kinds of forms of interim pressurization of being pressurised into; Described raising oxygen concentration is moment oxygen supplement, increases continuously and three kinds of modes of interim increase.
9. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure according to claim 8, it is characterized in that, described moment pressurization refers to: the internal tank air pressure was promoted in 1.1～1.5 barometric pressure range in 30 minutes, maximum is no more than 1.5 atmospheric pressure; Described continuous pressure refers to: per hour charged pressure percent is 0.1389～2.083% air in the container, and radix is 1 normal atmosphere, until the container inner air pressure is no longer to increase pressure in 1.1～1.5 normal atmosphere scopes the time; Described interim pressurization refers to: every 12 hours in the container charged pressure percent be 1.67～25% air, radix is 1 normal atmosphere equally, until the container inner air pressure is no longer to increase pressure in 1.1～1.5 normal atmosphere scopes the time;
Described moment oxygen supplement refers to: in 30 minutes, oxygen partial pressure is promoted to 50%;
Described increasing continuously refers to: oxygen concentration per hour increases by 48.61～166.67%, and radix is 10～15%, until oxygen concentration no longer increases when being 50%;
Described interim increasing refers to: oxygen concentration increased by 583.3～2000% in every 12 hours, until oxygen concentration no longer increases when reaching 50%.
10. the Bacterial cellulose liquid-absorbent material preparation method of a kind of sandwich structure according to claim 5, it is characterized in that, described reduction air pressure refers to: the air pressure that contacted with the bacteria cellulose film upper surface in container in 30 minutes is reduced to 1 normal atmosphere; Described reduction oxygen concentration refers to: in 30 minutes with container in oxygen concentration be reduced in 10～15% scopes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210575842.0A CN103083136B (en) | 2012-12-26 | 2012-12-26 | Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210575842.0A CN103083136B (en) | 2012-12-26 | 2012-12-26 | Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103083136A true CN103083136A (en) | 2013-05-08 |
| CN103083136B CN103083136B (en) | 2014-06-04 |
Family
ID=48196625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210575842.0A Expired - Fee Related CN103083136B (en) | 2012-12-26 | 2012-12-26 | Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103083136B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340722A (en) * | 2013-07-24 | 2013-10-09 | 海南光宇生物科技有限公司 | Sanitary towel good in water-absorbing performance |
| CN103349592A (en) * | 2013-07-25 | 2013-10-16 | 钟春燕 | Paper diaper with good water-absorbing quality |
| CN103392613A (en) * | 2013-07-24 | 2013-11-20 | 钟春燕 | Cat litter with high water absorption |
| CN104800089A (en) * | 2014-01-29 | 2015-07-29 | 娇芃生技股份有限公司 | Biological fiber composite dressing |
| US10682257B2 (en) | 2015-07-29 | 2020-06-16 | Evophancie Biotech Ltd | Biological fiber composite dressing |
| CN112513356A (en) * | 2018-09-27 | 2021-03-16 | 株式会社Lg化学 | Biological cellulose tablet and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008079034A2 (en) * | 2006-12-24 | 2008-07-03 | Politechnika Lodzka | A biomaterial composed of microbiological cellulose for internal use, a method of producing the biomaterial and the use of the biomaterial composed of microbiological cellulose in soft tissue surgery and bone surgery |
| JP2009024274A (en) * | 2007-07-19 | 2009-02-05 | Kenji Nakamura | Sheet for impregnation, and wet sheet product using the same |
| CN101509025A (en) * | 2009-03-20 | 2009-08-19 | 武汉科技学院 | Method of preparing bacteria cellulose composite material |
| US20110286948A1 (en) * | 2010-05-24 | 2011-11-24 | Nympheas International Biomaterial Corp. | Bacterial cellulose film and uses thereof |
-
2012
- 2012-12-26 CN CN201210575842.0A patent/CN103083136B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008079034A2 (en) * | 2006-12-24 | 2008-07-03 | Politechnika Lodzka | A biomaterial composed of microbiological cellulose for internal use, a method of producing the biomaterial and the use of the biomaterial composed of microbiological cellulose in soft tissue surgery and bone surgery |
| JP2009024274A (en) * | 2007-07-19 | 2009-02-05 | Kenji Nakamura | Sheet for impregnation, and wet sheet product using the same |
| CN101509025A (en) * | 2009-03-20 | 2009-08-19 | 武汉科技学院 | Method of preparing bacteria cellulose composite material |
| US20110286948A1 (en) * | 2010-05-24 | 2011-11-24 | Nympheas International Biomaterial Corp. | Bacterial cellulose film and uses thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340722A (en) * | 2013-07-24 | 2013-10-09 | 海南光宇生物科技有限公司 | Sanitary towel good in water-absorbing performance |
| CN103392613A (en) * | 2013-07-24 | 2013-11-20 | 钟春燕 | Cat litter with high water absorption |
| CN103340722B (en) * | 2013-07-24 | 2015-07-15 | 海南光宇生物科技有限公司 | Sanitary towel good in water-absorbing performance |
| CN103349592A (en) * | 2013-07-25 | 2013-10-16 | 钟春燕 | Paper diaper with good water-absorbing quality |
| CN103349592B (en) * | 2013-07-25 | 2015-08-05 | 钟春燕 | A kind of diaper of good water absorption |
| CN104800089A (en) * | 2014-01-29 | 2015-07-29 | 娇芃生技股份有限公司 | Biological fiber composite dressing |
| US10682257B2 (en) | 2015-07-29 | 2020-06-16 | Evophancie Biotech Ltd | Biological fiber composite dressing |
| CN112513356A (en) * | 2018-09-27 | 2021-03-16 | 株式会社Lg化学 | Biological cellulose tablet and preparation method thereof |
| EP3812494A4 (en) * | 2018-09-27 | 2021-08-18 | Lg Chem, Ltd. | Bio-cellulose sheet and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103083136B (en) | 2014-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103083136B (en) | Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material | |
| CN101921700B (en) | Device and method for preparing hollow heteromorphic bacteria cellulose material | |
| CN105311668B (en) | A kind of cuprous antiseptic dressing of bacteria cellulose combined oxidation and preparation method thereof | |
| CN105012158B (en) | Composite biological material stent and manufacturing method containing bacteria cellulose and fibroin albumen | |
| CN102973985A (en) | Porous bacterial cellulose skin repair material with density structure and preparation method thereof | |
| CN103233050B (en) | Bacterial cellulose membrane with gradient structure and preparation method thereof | |
| CN101905031B (en) | Method for preparing flamazine/bacterial cellulose composite wound dressing | |
| CN103083112B (en) | Bacterial cellulose skin regeneration material with density structure and preparation method thereof | |
| CN107737375A (en) | A kind of biology cellulose skin regeneration material and preparation method thereof | |
| CN101914434A (en) | Device and method for dynamically preparing heterocavity bacterium cellulose materials | |
| CN103007344B (en) | Hollow heterotype bacteria cellulose artificial blood vessel stent material with gradient structure and preparation method thereof | |
| CN110623792B (en) | Medical dressing and preparation method thereof | |
| CN103418018A (en) | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof | |
| CN104436291B (en) | A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer | |
| CN103007335A (en) | Method for preparing chitosan oligosaccharide/bacterial cellulose sponge | |
| CN100384485C (en) | Microbiological cellulose injury dressing for treating chronic injury | |
| CN201809342U (en) | Device for dynamically preparing profiled cavity bacterial cellulose material | |
| Salihu et al. | Overview of inexpensive production routes of bacterial cellulose and its applications in biomedical engineering | |
| WO2018106099A1 (en) | Method for producing bacterial cellulose membranes useful in biomedical applications | |
| CN107177048A (en) | A kind of bacteria cellulose polymeric lactic acid compound film and preparation method thereof and load medicine gauze and preparation method based on the composite membrane | |
| CN102991037B (en) | Bacteria cellulose controlled-release carrier material of sandwich structure and preparation method thereof | |
| CN103394113B (en) | Adhesive bandage | |
| CN110464876A (en) | A kind of growth factor-loaded bacteria cellulose/bioceramic composite membrane | |
| CN102994588B (en) | Cultural method for continuous thickening of bacterial cellulose | |
| CN101869516B (en) | Bacterial cellulose ice pack and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Shiyan Inventor after: Zhang Yun Inventor after: Yang Jingxuan Inventor after: Li Zhe Inventor after: Wang Liqun Inventor after: Zheng Yi Inventor after: Wang Huaping Inventor after: Zhou Hanyu Inventor before: Chen Shiyan Inventor before: Zhang Yun Inventor before: Yang Jingxuan Inventor before: Li Zhe Inventor before: Wang Liqun Inventor before: Zheng Yi Inventor before: Wang Huaping |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: CHEN SHIYAN ZHANG YUN YANG JINGXUAN LI ZHE WANG LIQUN ZHENG YI WANG HUAPING TO: CHEN SHIYAN ZHANG YUN YANG JINGXUAN LI ZHE WANG LIQUN ZHENG YI WANG HUAPING ZHOU HANYU |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140604 Termination date: 20211226 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |