CN103074261A - Composite flora and pulping method by using biological bacterial liquid of composite flora - Google Patents

Composite flora and pulping method by using biological bacterial liquid of composite flora Download PDF

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Publication number
CN103074261A
CN103074261A CN2012103431359A CN201210343135A CN103074261A CN 103074261 A CN103074261 A CN 103074261A CN 2012103431359 A CN2012103431359 A CN 2012103431359A CN 201210343135 A CN201210343135 A CN 201210343135A CN 103074261 A CN103074261 A CN 103074261A
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raw material
biological bacteria
pulping method
pulping
composite flora
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CN103074261B (en
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贾平
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BEIJING TIAN'AN BIOTECHNOLOGY CO., LTD.
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贾平
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The present invention relates to a composite flora and a pulping method by using a biological bacterial liquid of the composite flora. The composite flora comprises bacillus sp. with a preservation number of CGMCC No.5971, pseudomonas fluorescens with a preservation number of CGMCC No.5974, and Wickerhamomyces anomalus with a preservation number of CGMCC No.5975. The method mainly comprises: preparing a bacterial liquid; treating raw materials; and pulping, wherein pulping comprises: fiber kneading or squeezing rolling, biodegradation, steam sterilization, crude pulp grinding, fine pulp grinding, pulp material screening, soaking, and pulp washing. The paper board prepared by using the pulping method can achieve a level of more than an AA grade. In addition, waste liquid generated during a pulping process can be recycled or converted into biogas or an organic fertilizer adopted as an energy source so as to provide advantages of energy source saving and environment protection.

Description

A kind of composite flora and biological bacteria liquid pulping method thereof
Technical field
The present invention relates to a kind of biological bacteria liquid pulping method, relate in particular to a kind of composite flora and biological bacteria liquid pulping method thereof.
Background technology
Pulping technique is exactly with raw materials such as the stalk of plant, branch, trunks, removes wherein xylogen and the material such as pectin by degraded (or dissociating), obtains the process of fibrous matter.Fiber is the main component of papermaking.
In pulping process, isolate fiber, at first will be these non-fibrous material strippings, obtaining fiber is exactly the slurrying purpose.
According to the present situation of the present slurrying industry of China, mainly occupy an leading position the waste liquor contamination sublimity that produces during chemical pulp is produced, damage to land, polluted air in being applied to produce with chemical pulp; Add a large amount of sodium hydroxide (caustic soda) in slurrying, also use chemical harmful element in the bleaching process, producing waste liquid can't all recycle.And power consumption is high, and power consumption and water consumption are large; All using a large amount of electricity in whole production process, causing becomes the power consumption rich and influential family.The waste liquid blowdown flow rate is large; The waste liquid that can't recycle can only outwards discharge.Do not meet national energy-saving and reduce discharging policy.Material can not reach the Efficient Cycle recycling.Chemicals can't separate from waste liquid, and organism and chemicals mix, and organism also can't be reused, and causes a large amount of losses.
Therefore, be necessary to develop the bio-pulping technology, fundamentally solve the pollution difficult problem of pulping and papermaking industry.Although the bio-pulping of employing technology is also arranged in the prior art, but the biological bacteria that usually adopts is whiterot fungi or actinomycetes, although it makes moderate progress with respect to traditional chemical pulping technology, the defective that but the fiber fineness that still have long reaction time, makes is low, yield is low is difficult to be applied to actual production.Therefore, still need the further new biological bacteria of exploitation, improve bio-pulping process, overcoming defects, and realize simultaneously energy-saving and emission-reduction, water-saving reduces production costs and improves the rate of utilization of material.
Summary of the invention
The objective of the invention is to overcome the chemical pulping method of prior art and the defects of bio-pulping method, a kind of new slurrying biological bacteria is provided.The contriver has obtained to be suitable for the biological bacteria that can realize the foregoing invention purpose of bio-pulping by long screening operation.
Particularly, the invention provides a kind of composite flora, its comprise Pseudomonas fluorescens (Pseudomonas fluorescens) that the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number are CGMCC No.5974, this Wickerhamomyces anomalus bacterium of Wickerhamomyces anomalus(that preserving number is CGMCC No.5975 in malt juice liquid medium 25 ℃ cultivated three days, cell is spherical, avette, sausage shape, size is (4.8-14.4) * (3.6-7.2) μ m, has precipitation to form.25 ℃ of cultivations in wort agar inclined-plane one month, bacterium colony cheese shape, oyster white, surface smoothing, not reflective, edge root shape.Corn Meal Agar Dalmau is dull and stereotyped to be cultivated, and produces pseudohypha).
Described composite flora can be used in the slurrying of biological bacteria liquid.
The present invention also provides a kind of biological bacteria liquid pulping method, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-3:1-2:2-3;
2) raw material is processed: woody raw material is removed the peel rear segment or section, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) slurrying, it comprises step:
Discongest: with raw material thread rolling and/or crowded stone roller of swollen;
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
The corase grind slurry: the raw material after will sterilizing carries out one section corase grind slurry, becomes fibrous bundle;
Fine grinding slurry: above-mentioned one section corase grind slurry is carried out two sections fine grinding slurries, make fibrous bundle disperse into single fiber;
Slurry screening: screening, filter through the fibrous bundle in the slurry behind one section corase grind slurry and the two sections fine grindings slurries, defibrination makes it make single fiber again;
Latent, pulp washing disappears: the above-mentioned paper pulp that makes is soaked in warm water, be used for cardboard processed.
The density of the bacterium liquid that wherein, forms in the step 1) be 6,000 ten thousand/more than the ml bacterium.
Wherein, step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.The described swollen time is 10-12h.
Wherein, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 32-36 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, the soak solution step 2) behind the immersion raw material is through flocculating, precipitate, supernatant liquor recycling, throw out input methane-generating pit fermentation generation biogas.
The advantage of biological bacteria liquid pulping method provided by the invention is: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, and zero pollutes.2) bio-pulping can play a protective role to fiber, compares with traditional chemical process, and present method can all reclaim full fiber and half fiber, has therefore improved slurry yield.Reduce energy consumption 30% than traditional slurrying; Paper pulp yield improves 20-25%.3) bio-pulping is to carry out lignin degradation under normal pressure, energy-conservation, reduction of discharging, low-carbon (LC).4) production cost is low, and economic benefit is high.Adopt the cardboard of this biological bacteria liquid pulping method preparation to reach the above level of AA level.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns second stage employ, remakes presoak and uses.Contain gas chromatography and the nutrient for plants such as N, P, K such as abundant xylogen in the throw out, throw out mixes with old bacterium liquid (the raw material thickness bacterium liquid of repeatedly degrading also contains N, P, K, Fe and trace element) again, acidifying, then together enter in the marsh gas fermentation pool, produce biogas.With the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, the granulation fertilizer dispatches from the factory at last, realizes zero release.
The biological flora of the present invention by providing above-mentioned contriver to obtain through long creation work, further perfect existing bio-pulping technology, this technology reduced the reaction times, improved the purity and the yield that make fiber, so that can be applied in actual production on a large scale.Applying biological bacterium of the present invention at short notice in the degrading plant body xylogen produce paper pulp, papermaking, the byproduct twice transformation becomes biogas, biogas coal supply, gas boiler for dual purpose combustion heating are saved coal consumption.At last, the natural pond slag is made organic fertilizer, has formed the economic circulation new model of " material organic transformation ", reaches the no waste discharging, namely zero release.Fundamentally solved the pollution difficult problem of pulping and papermaking industry.Energy-saving and emission-reduction, water-saving has reduced production cost, has improved the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and cooperation accompanying drawing are described in detail below.
Description of drawings
Fig. 1 is the schema according to the biological bacteria liquid pulping method of one embodiment of the invention;
Fig. 2 is the schema of biological bacteria liquid pulping method according to another embodiment of the present invention.
Embodiment
The configuration of embodiment 1 bacterium liquid
The biological flora that adopts among the present invention on April 6th, 2012 at the common micro-organisms center (CGMCC of China Committee for Culture Collection of Microorganisms, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is that Pseudomonas fluorescens (Pseudomonas fluorescens), the preserving number of CGMCC No.5974 is the Wickerhamomyces anomalus of CGMCC No.5975.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-3:1-2:2-3; The density of the bacterium liquid that forms be 6,000 ten thousand/more than the ml bacterium, for subsequent use.
The biological bacteria liquid slurrying of embodiment 2 woody raw materials
Take caragana microphylla as raw material, specify the biological bacteria liquid pulping method when adopting woody raw material.All the other woody raw materials, for example the bio-pulping method of willow, eucalyptus can be carried out with reference to this technique.When wherein adopting caragana microphylla, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:2:2; When adopting willow, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:2:2; When adopting eucalyptus, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:2:3.
Specifically please refer to Fig. 1, the flow process of biological bacteria liquid pulping method is divided into three phases: preparatory stage, slurrying workshop section and byproduct workshop section.
(1) preparatory stage: 1-4
1)-2): the caragana microphylla that harvesting is returned carries out the separation of skin bar.Adopt winnowing machine or other mechanical separation all can.After the skin bar separates, skin is inputted organic feed workshop processing feed, the bar after the decortication is inputted next program.
3) with bar segment on cutting machine, length is 3-4 ㎝, and angle is for well, to increase infiltrating area.
4) the branch section input after will peeling soak in storehouse or the fermentation vat wash, cold soaking, at first with foreign material flush awaies such as raw material appearance earth, soak simultaneously, water temperature is natural temperature, the time with soak into, swollen is as the criterion 10-12h.After the liquid muddiness after repeatedly soaking, supernatant liquor can also reuse after flocculating, precipitating.Biogas is produced in the fermentation of throw out input methane-generating pit.
More than four the step can be that intermittent type also can be linkage type.
(2) slurrying workshop section: 5-12
5) discongest: with the branch section input thread-rolling machine of swollen or squeeze grind, kneading machine comes the innovation wood segment structure, makes it loose one-tenth wood wool shape, is conducive to the biological bacteria infiltration, brings into play its Degradation.
6) biological degradation: in the raw material input biological bacteria degraded storehouse or tank after will discongesting, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6, and temperature remains on 35-40 ℃, time 32-36 hour.The xylogen degradation reaction occurs under the condition of biological bacteria, brings into play its single-minded effect.
7) wet sterilization: after raw materials pretreatment is finished, with raw material from bacterium liquid, pull out, draining, be input in the steam storehouse, pass into water at atmospheric pressure steam 10-30 minute sterilization.Paste roller mill is inputted with raw material in the lower end.
8) corase grind slurry: carry out one section corase grind slurry in the raw material input high-density paste mill after will sterilizing, become coarse fiber bundle.
9) fine grinding slurry: enter again high-density paste mill from the coarse particles of above-mentioned output and carry out the fine grinding slurry, make fibrous bundle disperse into single fiber.
10) slurry screening: in the slurry behind two sections defibrinations, contain the minority fibrous bundle, need through screening, filter tow, defibrination has reached single fiber again.
11) latent, the pulp washing that disappears: the paper pulp behind corase grind slurry and fine grinding slurry, be subjected to mechanical friction, major part is bended, torsional deformation, soaks the fiber deflection that the elimination defibrination causes in warm water, makes it to unfold.
12) cardboard processed: with the pulping liquor of above-mentioned preparation input paper machine, cardboard processed after copying the technical process such as paper.
(3) byproduct stage: A-C
Isolated caragana microphylla skin contains abundant nutritive ingredient among the A, 3, and fermentation changes into ox, sheep feed.
After the liquid muddiness through repeatedly soaking, washing among the B, 4, through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into the coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: natural pond slag, natural pond liquid after the methane-generating pit fermentation are the biological organic fertilizers that enriches, and liquid topdresses as farm crop and flower nutrient solution uses, and the solid granulation is made base manure and used, and all is green fertilizers.
Paper physical property index determining result please see following table 1 for details behind the defibrination, and every data have all reached excellent fluting board AA level level (table 3):
Table 1
Figure BDA00002147970600071
The biological bacteria liquid slurrying of embodiment 3 draft raw materials
Take wheat straw as raw material, specify the biological bacteria liquid pulping method when adopting the draft raw material.All the other draft raw materials, for example the bio-pulping method of wheat straw, straw, cornstalk can be carried out with reference to this technique.
When wherein adopting wheat straw, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:2:1:2:3; When adopting straw, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1:2:3; When adopting cornstalk, composite flora is configured according to following mass ratio: genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:1:3.
Specifically please refer to Fig. 2, the flow process of biological bacteria liquid pulping method is divided into three phases: preparatory stage, slurrying workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
The segment that wheat straw is cut into 4-5cm, input soak in storehouse or the fermentation vat wash, cold soaking, at first with foreign material flush awaies such as raw material appearance earth, soak simultaneously, water temperature is natural temperature, the time with soak into, swollen is as the criterion 10-12h.After the liquid muddiness after repeatedly soaking, supernatant liquor can also reuse after flocculating, precipitating.Biogas is produced in the fermentation of throw out input methane-generating pit.
(2) slurrying workshop section: 5-12 and (three) byproduct stage: A-C are with embodiment 2, and wherein employed biological bacteria such as embodiment 1 do not repeat them here, and wherein during biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.
Paper physical property index determining result please see following table 2 for details behind the defibrination.
Table 2
Figure BDA00002147970600081
The quality index of table 3 Corrugating Base Paper
Figure BDA00002147970600091
Although the present invention discloses as above with preferred embodiment; so it is not to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when can doing a little change and improvement, so protection scope of the present invention is as the criterion when looking the claim person of defining.

Claims (10)

1. composite flora, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is that Pseudomonas fluorescens (Pseudomonas fluorescens), the preserving number of CGMCC No.5974 is the Wickerhamomyces anomalus of CGMCC No.5975.
2. the application of composite flora claimed in claim 1 in the slurrying of biological bacteria liquid.
3. a biological bacteria liquid pulping method is characterized in that, comprises step:
1) configuration of bacterium liquid: composite flora claimed in claim 1 is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: Pseudomonas fluorescens: Wickerhamomyces anomalus is 1-3:1-2:2-3;
2) raw material is processed: will cut into slices after the woody raw material peeling, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) slurrying, it comprises step:
Discongest: with raw material thread rolling and/or crowded stone roller of swollen;
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
The corase grind slurry: the raw material after will sterilizing carries out one section corase grind slurry, becomes fibrous bundle;
Fine grinding slurry: above-mentioned one section corase grind slurry is carried out two sections fine grinding slurries, make fibrous bundle disperse into single fiber;
Slurry screening: screening, filter through the fibrous bundle in the slurry behind one section corase grind slurry and the two sections fine grindings slurries, defibrination makes it make single fiber again;
Latent, pulp washing disappears: the above-mentioned paper pulp that makes is soaked in warm water, be used for cardboard processed.
4. biological bacteria liquid pulping method according to claim 3 is characterized in that, the density of the bacterium liquid that forms in the step 1) be 6,000 ten thousand/more than the ml bacterium.
5. biological bacteria liquid pulping method according to claim 3 is characterized in that step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.
6. according to claim 3 or 5 described biological bacteria liquid pulping methods, it is characterized in that step 2) the described swollen time is 10-12h.
7. biological bacteria liquid pulping method according to claim 3 is characterized in that, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 32-36 hour.
8. biological bacteria liquid pulping method according to claim 3 is characterized in that, in the described biological degradation of step 3), the mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.
9. biological bacteria liquid pulping method according to claim 3 is characterized in that, the described steam sterilizing of step 3) is water at atmospheric pressure steam sterilizing 10-30 minute.
10. biological bacteria liquid pulping method according to claim 3 is characterized in that step 2) in soak soak solution behind the raw material through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
CN201210343135.9A 2012-09-17 2012-09-17 Composite flora and pulping method by using biological bacterial liquid of composite flora Expired - Fee Related CN103074261B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014040393A1 (en) * 2012-09-17 2014-03-20 北京天安生物科技有限公司 Complex microbial flora and application thereof

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Publication number Priority date Publication date Assignee Title
CN101838613A (en) * 2009-09-30 2010-09-22 河南省恒隆态生物工程股份有限公司 Agricultural straw fast-degradation decomposition agent strain composition
CN102002468A (en) * 2010-08-13 2011-04-06 东华大学 Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof
CN102174399A (en) * 2010-12-31 2011-09-07 广西赛尔纸业有限公司 Method for preparing herbal biological fiber and biological paper pulp

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
CN101838613A (en) * 2009-09-30 2010-09-22 河南省恒隆态生物工程股份有限公司 Agricultural straw fast-degradation decomposition agent strain composition
CN102002468A (en) * 2010-08-13 2011-04-06 东华大学 Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof
CN102174399A (en) * 2010-12-31 2011-09-07 广西赛尔纸业有限公司 Method for preparing herbal biological fiber and biological paper pulp

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014040393A1 (en) * 2012-09-17 2014-03-20 北京天安生物科技有限公司 Complex microbial flora and application thereof

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