CN103070052A - Method for breeding regeneration plants by rice dormant buds - Google Patents
Method for breeding regeneration plants by rice dormant buds Download PDFInfo
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Abstract
The invention discloses a method for breeding regeneration plants by rice dormant buds, and belongs to the technical field of crop seedling. According to the method, rice straws of rice with reciprocal 1 to reciprocal 3 sections of dormant buds are taken to be scattered and planted onto a seedling culture bed, soil is covered, or the rice straws are soaked by plant hormone, then, the rice straws are scattered and planted onto the seedling culture bed, the soil is covered, the seedling culture and the management are carried out according to the conventional seedling culture method, and the seedlings are transplanted to the field after the regeneration seedlings reach 3-leaf to 4-leaf period. The method has the advantages that the effective storage and breeding can be realized for obtaining a large number of special rice materials including female sterile, male sterile and the like, the difficult problem of breeding difficulty of special materials is solved, hybrid F1 can be directly utilized for obtaining a large number of F1 regeneration plants, the rice hybrid seed production cost can be effectively reduced, the breeding period is shortened, and the hybrid advantages can be perfectly maintained. According to the method, the rice overground parts with rice straws with reciprocal 1 to reciprocal 3 sections of dormant buds are adopted, both the regeneration efficiency of the rice straws treated by hormone and the regeneration efficiency of the rice straws which are not treated by hormone are obviously superior to that of rice pile direct regeneration, the method is simple, the implementation is easy, and the popularization and the application are convenient.
Description
Technical field
The present invention relates to a kind of method of utilizing the paddy rice sleeping bud to obtain regeneration plant, belong to agricultural biological technical field, more particularly belong to the plant breeding technique field.
Background technology
Paddy rice (
Oryza sativa.L) be the third place in the world generalized grain crop that is only second to corn and wheat, it is as staple food take rice that population over half is arranged in the world.China is world paddy rice production and consumption big country, and the rice cropping area accounts for 1/3 of plant of grain crops area, and output accounts for the nearly 1/2 of total output of grain, and per capita year more than the consumption rice 150kg, so Rice Production is concerning the safe overall situation of national food.Constantly perfect along with modern Rice Production technology all obtained achievement highly visible in the raising of yield of brown rice and quality, alleviated greatly the pressure of crisis in food.But since 20 end of the centurys, population in the world is in the situation of rapid growth always, causes to begin now to occur consumption figure greater than output, and stockpile number atrophy, price are unprecedented soaring.Therefore in the face of so severe population problem, grain-production shoulders heavy responsibilities.
Ensure the grain yield steady-state growth, just need constantly to excavate by all means new germ plasm resource, open up new production technology, seek economy and efficient production model.Therefore realize this goal, obtain new breakthrough with regard to needing at the production chain of paddy rice.Discovery and the further investigation of the paddy female sterile mutant material of the development and use of Exploration of Wild Rice Germplasm Resources, the spread of ratoon rice, Dominant gene, and take these a series of researchs such as new crossbreeding pattern of seeking all as we provide good thinking of development.
The breeding of paddy rice also depends on sexual propagation and vegetative propagation as other plant.Sexual propagation is as its topmost modes of reproduction, and production and the breeding of paddy rice all played decisive role.Utilization and the development of male sterility of rice (male sterility, MS) and paddy female sterile (femalesterility, FS), hybrid rice is the Important Action of Ensuring Food Safety.At present, in breeding of hybridized rice, development and the technical system of utilizing mainly contain three line method and two line method, although these systems are all comparatively ripe, but remain some shortcomings, be mainly reflected in following some: 1. Parent branch or subregion plantation, must manually catch up with the powder supplementary pollination when blooming, Parent people work point is received during results, time-consuming taking a lot of work; 2. hybrid seed yield is low, can not fine solution impurity of seeds problem; 3. be not suitable for scale, mechanized operation etc.Based on this, visible crossbreeding process also needs to expend huge material resources, manpower and very long breeding cycle, could seek so a kind of with F
1Be material, means by vegetative propagation technique, obtain fast the production of hybrid seeds pattern of large batch of hybrid strain? along with the application of paddy female sterile in breeding, utilize male sterile (MS) and female sterile (FS), make up the production of hybrid seeds pattern of " MS+FS ", pollen donor when being the production of hybrid seeds is Female sterile clone, and also being expected to soon follow-up three is that another Breeding Model with double-line hybrid carries out large-scale promotion and application.But it is Female sterile clone that problem is its male parent, can not be normally solid to obtain large batch of female sterile strain.Therefore, can the application of succeeding of this pattern be depended on obtain a large amount of female sterile strains.Breeding also has some special rare your materials (such as monomer and trisome and polyploid, the material such as selfing is not affine), owing to be bred or be difficult to the characteristics of self can not by amphigenetic mode.Vegetative propagation technique and gene engineering etc. are how to obtain the most frequently used method and approach of these special materials at present.
The vegetative propagation of plant mainly contains cuttage, grafting, press strip and tissue cultivation etc., cultivates being most widely used in paddy rice with tissue.Also have larger limitation but rice tissue is cultivated: it draws materials rataria, young fringe, pollen and is subjected to season and environmental limit, and the time of drawing materials is strict, too early or spend evening and all can cause the callus of induce failure; Easily occur the situations such as death, albefaction in the tissue culture procedures, be difficult to obtain a large amount of group training seedlings, and group training seedling relatively a little less than, be transplanted to the land for growing field crops survival rate and can not get ensureing, the while is high for large-scale production cost.
Wild rice is made the important materials of excavating resource as recent rice, more and more be subject to researcher's attention.Common wild-rice is the nearly edge ancestors of cultivated rice, and it becomes modern cultivated rice through all the year round evolution.Cultivated rice is generally annual, and common wild-rice is herbaceos perennial, has stronger reviviscence.The application that the ratoon rice that utilizes this characteristic to grow up has now had certain scale has obtained appreciable achievement.At present, approximately 1,100 ten thousand mu of China's ratoon rice cultivated areas are the countries that develops in the world ratoon rice area maximum.But the ratoon rice plant height, tiller, the proterties such as spike length, Number of kernels compared first rice crop and all relatively fallen sharply, and causes average unit yield not high, output is unstable.At present rice regeneration strain does not only take full advantage of its sleeping bud regeneration capacity only for directly utilize rice stub regeneration in the field, and output and productivity effect are also generally on the low side.Utilize the reproducing characteristic of paddy rice sleeping bud to breed aftergrowth, also do not have a cover can obtain fast the production technology system of regeneration strain.Reasonably excavate paddy rice sleeping bud regeneration capacity, obtain regeneration plant and make up perfect culture technique system in conjunction with plant hormone and the regeneration of growth regulator induction paddy rice sleeping bud, the research of this respect is still waiting further deeply.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing rice regeneration strain only for directly utilize rice stub regeneration in the field, output is unstable, output and productivity effect are generally on the low side, and the production cost of hybrid rice is high and production of hybrid seeds process is loaded down with trivial details, the breeding difficulty of special material, paddy rice group training seedling death, albefaction and group training seedling easily occur and is transplanted to the land for growing field crops survival rate a little less than relatively and can not get ensureing, there are the high defective of large-scale production cost and deficiency, the purpose of this invention is to provide efficient, quick, the low-cost paddy rice mating system that obtains the rice regeneration strain of a kind of energy.
Paddy rice sleeping bud provided by the present invention is bred the method for regeneration plant, may further comprise the steps:
(1) gathered in rear 7 days at first rice crop, intercepting first rice crop acrial part is with falling 1～rice straw of 3 node dormancy buds;
(2) be spread out on the seedling bed and earthing after the described rice straw of step (1) is cleaned, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling, or the described rice straw of step (1) is cleaned rear with plant hormone composition immersion 40～50 hours, be spread out over again on the seedling bed and earthing, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling;
(3) treat that regrowth 3 leaves～4 leaf phases transplant the land for growing field crops, carry out the field production management by the conventional field production management method of paddy rice.
The prescription of described plant hormone composition is 0.25mg/L 6-BA+30 mg/L～60mg/L NAA, 0.1mg/L NAA+10.0 mg/L～12.0mg/L 6-BA or 10mg/L Kinetin+2.0 mg/L～5.0mg/L NAA.
The prescription of described plant hormone composition is 0.25mg/L 6-BA+30mg/L NAA.
One of feature of the present invention is the sleeping bud that utilizes on the paddy rice rice straw node, makes up the technical system that obtains fast a large amount of rice regeneration plant.Sleeping bud also claims axillalry bud, resting bud or regeneration bud, is the basis of rice regeneration.
Second feature of the present invention is to induce in conjunction with plant hormone, improves Dormant Buds efficient and promotes growing of regrowth.Plant hormone (Plant hormone) is that some are synthetic in plant corpus, can be delivered to site of action from producing the position, and micro-concentrations can produce to plant corpus the acitve organic matter of certain physiological action.It can also be the similar chemical substance (plant growth regulator) of artificial composite structure effect.Many plant hormones are to the regeneration of paddy rice and grow influentially, and the Cell Differentiation of promotion, division, growth are arranged, and the transportation of nutriment is regulated in the dormancy of breaking dormancy bud, also is beneficial to effects such as inducing root and adventive root formation simultaneously.
Compared with prior art, the invention has the beneficial effects as follows:
⑴ can effectively preserve and breed and obtain a large amount of special rice material (, monomer not affine such as female sterile, male sterile, selfing and polyploid etc.), solved the difficult problem that special material is difficult for breeding.
⑵ directly utilize hybrid F
1Just can obtain a large amount of F
1Regeneration plant does not need to carry out hybrid seeding repeatedly, not only effectively reduces the cost of the paddy rice cross breeding production of hybrid seeds and shortens breeding cycle, can also well keep its hybrid vigour.
⑶ use the germination efficiency of the sleeping bud that can improve the relatively relatively poor kind of regeneration power and promote the newborn development of root of node in conjunction with plant hormone because the regeneration capacity of different materials is different, well strengthened the scope of application of the method.
(4) asexual reproduction method set up of the present invention, for special rice material (as femalely educate, female sterile, male sterile etc.) and conventional Rice, hybrid rice (hybrid F
1) adopt with falling 1～fall the rice straw of 3 node dormancy buds with HORMONE TREATMENT and need not hormone-treated regeneration efficiency all extremely significantly be better than using the rice stub Direct Regeneration, its emergence rate, leaf area index, plant height, Number of kernels geometric ratio contrast and increase substantially, difference is extremely remarkable.Need not hormone-treated method, it is emerged efficient and improves 68.7%～91.2% than the efficient of emerging of directly transplanting rice stub, and Number of kernels is than the Number of kernels improve 105.5%～158.2% of directly transplanting rice stub; Use hormone-treated method, its emergence rate has improved 112.3%～132.9% than the emergence rate of the contrast of direct transplanting rice pile material, and grain husk spends number to improve 107.1%～138.3% than the contrast of direct transplanting rice pile material, has all produced unforeseeable technique effect.
(5) the inventive method is simple and easy implements, easy to utilize.
Description of drawings
Fig. 1 is the emergence rate statistical chart that first round hormon, different hormone combinations thing and variable concentrations are processed rice material, and abscissa represents the concentration of hormone and hormonal composition among the figure, and ordinate represents the emergence rate of rice material, curve among Figure 1A
Emergence rate, curve that the expression rice material is processed with variable concentrations NAA
Emergence rate, curve that the expression rice material is processed with variable concentrations KIN
Emergence rate, curve that the expression rice material is processed with variable concentrations 6-BA
The emergence rate that the expression rice material is processed with KN hormonal composition listed in the table 2; Curve among Figure 1B
The emergence rate that the expression rice material is processed with DB hormonal composition listed in the table 2, curve
The emergence rate that the expression rice material is processed with NB hormonal composition listed in the table 2, curve
The emergence rate that the expression rice material is processed with DK hormonal composition listed in the table 2, curve
The emergence rate that the expression rice material is processed with NK hormonal composition listed in the table 2.
Fig. 2 second takes turns the emergence rate statistical chart of processing rice material with DK hormonal composition variable concentrations, and abscissa represents the concentration of DK hormonal composition among the figure, and ordinate represents to process the emergence rate of rice material, curve
The emergence rate that expression fs processes with hormon concentration listed in the table 3, curve
The emergence rate that expression FS processes with hormon concentration listed in the table 3, curve
The emergence rate that expression CMS processes with hormon concentration listed in the table 3, curve
The emergence rate that expression multitude elm B processes with hormon concentration listed in the table 3, curve
The emergence rate that listed hormon concentration is processed in the assorted 37 usefulness tables 3 in expression Yunnan.
Fig. 3 second takes turns the emergence rate statistical chart of processing rice material with NB hormonal composition variable concentrations, and abscissa represents the concentration of NB hormonal composition among the figure, and ordinate represents to process the emergence rate of rice material, curve
The emergence rate that expression fs processes with hormon concentration listed in the table 4, curve
The emergence rate that expression FS processes with hormon concentration listed in the table 4, curve
The emergence rate that expression CMS processes with hormon concentration listed in the table 4, curve
The emergence rate that expression multitude elm B processes with hormon concentration listed in the table 4, curve
The emergence rate that listed hormon concentration is processed in the assorted 37 usefulness tables 4 in expression Yunnan.
Fig. 4 second takes turns the emergence rate statistical chart of processing rice material with BN hormonal composition variable concentrations, and abscissa represents the concentration of BN hormonal composition among the figure, and ordinate represents the emergence rate of rice material, curve
The emergence rate that expression fs processes with hormon concentration listed in the table 5, curve
The emergence rate that expression FS processes with hormon concentration listed in the table 5, curve
The emergence rate that expression CMS processes with hormon concentration listed in the table 5, curve
The emergence rate that expression multitude elm B processes with hormon concentration listed in the table 5, curve
The emergence rate that listed hormon concentration is processed in the assorted 37 usefulness tables 5 in expression Yunnan.
Fig. 5 second takes turns the emergence rate statistical chart of processing rice material with KN hormonal composition variable concentrations, and abscissa represents the concentration of KN hormonal composition among the figure, and ordinate represents the emergence rate of rice material, curve
The emergence rate that expression fs processes with hormon concentration listed in the table 6, curve
The emergence rate that expression FS processes with hormon concentration listed in the table 6, curve
The emergence rate that expression CMS processes with hormon concentration listed in the table 6, curve
The emergence rate that expression multitude elm B processes with hormon concentration listed in the table 6, curve
The emergence rate that listed hormon concentration is processed in the assorted 37 usefulness tables 6 in expression Yunnan.
Embodiment is to disseminate with vegetative approach with the sleeping bud of first rice crop rice straw Nodes to cultivate regeneration or regenerate in conjunction with the plant hormone induction culturing, the breeding of regenerating of the final method that obtains the recycled water rice plants, take seed sprouting, direct rice stub of transplanting as contrast, compare their difference on each economical character.
Below each case study on implementation three above repeated experiments all are set; Used experiment material and reagent all can commercially be bought and obtain.
Embodiment 1
Select FS (female educating), fs(female sterile), CMS(cytoplasmic male sterility cytoplasmic male sterility), multitude elm B(conventional material), the assorted 37(hybrid F in Yunnan
1) be test material, to disseminate earthing with the rice straw of node dormancy bud cultivates, and direct transplanting rice stub is set for contrasting, to the emergence rate in seedling stage, maturing stage is carried out the investigation such as plant height, spike length, Number of kernels, 100-grain weight, investigates the inventive method to advantages such as the efficient of emerging such as some paddy rice special materials, conventional material, hybrid rices.
Concrete grammar is as follows:
(1) gathered in rear 7 days at FS, fs, CMS, multitude elm B, assorted 37 first rice crops in Yunnan, intercepting FS, fs, CMS, multitude elm B, the assorted 37 first rice crop acrial parts in Yunnan are cleaned with falling 1～rice straw of 3 node dormancy buds; (described first rice crop acrial part with fall 1～specifically the drawing materials of rice straw of 3 node dormancy buds of falling is to get more than the earth's surface the approximately rice straw of 10～15cm, it is with falling 1 and fall the rice straw of two node dormancy buds of 2 nodes, or with falling 1, fall 2 and fall the rice straw of three node dormancy buds of 3 nodes, consider practical condition, this test with above-mentioned with falling 1 and fall two node dormancy buds of 2 nodes and with falling 1, fall 2 and fall the same material of three node dormancy buds of 3 nodes and be mixed and grow seedlings, below each embodiment with fall 1～fall 3 node dormancy buds rice straw specifically draw materials all identical with the present embodiment);
(2) above-mentioned rice straw with the node dormancy bud evenly is spread out over earthing on the seedling bed by residential quarter of same material, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling, transplant simultaneously FS rice stub, fs rice stub, CMS rice stub, multitude elm B rice stub, assorted 37 rice stubs in Yunnan to identical seedling bed, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling, in contrast;
(3) regrowth that will process and contrast is in the 3 leaves-4 leaf phase statistical comparison efficient of emerging respectively, and the Transplanting Test field, carries out the field production management by the conventional field production management method of paddy rice.
Result of the test is found (table 1): utilize the inventive method will with fall 1～that the rice straw of 3 node dormancy buds of falling is disseminated the efficient of emerging in seedling stage that earthing cultivates is more much higher than the efficient of emerging of directly transplanting rice stub, its emergence rate: FS improves 68.7% than contrast, fs improves 73.8% than contrast, CMS improves 80.4% than contrast, multitude elm B improves 80.3% than contrast, and the assorted 37 ratio contrasts in Yunnan improve 91.2%; Its Number of kernels (grain/fringe): FS improves the contrast of 158.2%, fs ratio than contrast and improves 146.6%, CMS than contrast raising 146.9%, and multitude elm B improves 108.6% than contrast, and the assorted 37 ratio contrasts in Yunnan improve 105.5%.Be about to fall 1～rice straw of 3 node dormancy buds of falling disseminates earthing and cultivates, it emerges efficient than the efficient raising 68.7%～91.2% of emerging of directly transplanting rice stub, its Number of kernels is than the Number of kernels improve 105.5%～158.2% of directly transplanting rice stub, show the inventive method for special rice material (as femalely educate, female sterile, male sterile etc.) and conventional Rice, hybrid F
1Employing with fall 1～rice straw of 3 node dormancy buds of falling breeds the acquisition regeneration plant, its emergence rate, Number of kernels and respective material are directly transplanted rice stub and are compared and all produced unforeseeable technique effect.
Select FS, fs, CMS, multitude elm B, Yunnan assorted 37 to be test material, utilize the KIN(kinetin of different gradients), the 6-BA(6-benzyladenine), NAA (methyl α-naphthyl acetate)+KIN, 2,4-D(2, the 4-dichlorphenoxyacetic acid)+KIN, NAA+6-BA, 2, the 4-D+6-BA solution concentration is processed, the projects such as emergence rate are investigated, compare the situations such as emergence rate under hormon, different hormone combinations and the variable concentrations, through the screening of number wheel, obtain suitable hormone, hormone combinations and concentration ratio.
Concrete grammar is as described below:
(1) gathered in rear 7 days at FS, fs, CMS, multitude elm B, assorted 37 first rice crops in Yunnan, intercepting FS, fs, CMS, multitude elm B, the assorted 37 first rice crop acrial parts in Yunnan are cleaned with falling 1～rice straw of 3 node dormancy buds;
(2) rice straw in the described FS of mixed in equal amounts step (1), fs, CMS, multitude elm B, Yunnan assorted 37, hormone, the hormonal composition with the variable concentrations shown in the table 2 soaks the 48h processing respectively;
(3) evenly be spread out over earthing on the seedling bed with the rice straw of node dormancy bud after step (2) is processed, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling;
(4) treat that the regrowth 3 leaves-4 leaf phase adds up respectively emergence rate, just sift out preferably hormone, hormonal composition and concentration (see figure 1).
Table 2 note:
1, the used plant hormone of table 2 and plant hormone composition abbreviation:
Kinetin（KIN）、6-BA（BA）、0.1mg/LNAA+Kinetin（NK）、0.1mg/L?2,4-D+Kinetin（DK）、0.1mg/L?NAA+6-BA（NB）、0.1mg/L?2,4-D+6-BA（DB）、10mg/L?Kinetin+?NAA（KN）；
2, other symbolic representation of table 2:
KIN1～KIN6, BA1～BA6, NAA1～NAA6 represent that respectively hormone Kinetin, 6-BA, NAA variable concentrations gradient are followed successively by: 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L;
NK1～NK6 represents that respectively among hormonal composition 0.1mg/L NAA+Kinetin, the concentration quantitative of NAA is 0.1mg/L, and the change in concentration gradient of KIN is followed successively by 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L in this hormonal composition;
DK1～DK6 represents respectively hormonal composition 0.1mg/L2, and among the 4-D+Kinetin, the concentration quantitative of 2,4-D is 0.1mg/L, and the change in concentration gradient of KIN is followed successively by 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L in this hormonal composition;
NB1～NB6 represents that respectively among hormonal composition 0.1mg/L NAA+6-BA, the concentration quantitative of NAA is 0.1mg/L, and the change in concentration gradient of 6-BA is followed successively by 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L in this hormonal composition;
DB1～DB6 represents respectively hormonal composition 0.1mg/L 2, and among 4-D+6-BA, the concentration quantitative of 2,4-D is 0.1mg/L, and the change in concentration gradient of 6-BA is followed successively by 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L in this hormonal composition;
KN1～KN6 represents that respectively the concentration quantitative of KIN is 10mg/L among the hormonal composition 10mg/L Kinetin+ NAA, and the change in concentration gradient of NAA is followed successively by 1mg/L, 3mg/L, 6mg/L, 9mg/L, 12mg/L, 15mg/L in this hormonal composition.
Result is found (Fig. 1):
When using single hormone, along with the raising of hormone concentration, emergence rate all is parabola trend.Use the Kinetin emergence rate to rise along with the rising of concentration, be about 36% the 10mg/L emergence rate is the highest, descend afterwards; Use the 6-BA emergence rate to raise along with the rising of concentration, when concentration was 9mg/L, emergence rate was up to 37%, and the rising emergence rate along with concentration sharply reduces afterwards; Use the NAA emergence rate to raise along with the rising of concentration, emergence rate reaches 38% when concentration is 15mg/L, and the trend that increases in addition along with the rising emergence rate of concentration.
DB(is hormonal composition: 0.1mg/L 2, and 4-D+6-BA), NK(0.1mg/L NAA+Kinetin) emergence rate all is lower than 40%, induces emergence rate all undesirable.
KN(10mg/L Kinetin+ NAA) in the hormone combinations take the KIN of 10.0mg/L as quantitatively, the concentration of NAA is higher at 1.0mg/L～6.0mg/L emergence rate, all is higher than 40%, has the good efficient of inducing, concentration along with NAA raises afterwards, and emergence rate obviously reduces.
DK(0.1mg/L2,4-D+Kinetin) in the hormone combinations with 2 of 0.1mg/L, 4-D is that quantitatively the concentration of Kinetin is higher at 2.0mg/L～4.0mg/L emergence rate, all is higher than 40%, along with the concentration of Kinetin raises, emergence rate obviously reduces.
Take the NAA of 0.1mg/L as quantitatively, the concentration of 6-BA is higher at 7.0mg/L～12.0mg/L emergence rate, all is higher than 40% in the hormone combinations of NB(0.1mg/L NAA+6-BA), and the concentration along with 6-BA raises afterwards, and emergence rate obviously reduces.
Therefore, the result of primary dcreening operation:
When 1. being used alone hormone, emergence rate was up to 36% when Kinetin concentration was 10mg/L, and other plant hormone capable of being combined further screens; Emergence rate reached 38% when NAA concentration was 15mg/L, and the trend that increases in addition along with the rising emergence rate of concentration, can continue the screening of higher concentration; Emergence rate all was higher than 30% when 6-BA concentration was 1mg/L～10mg/L, and emergence rate is up to 37% when 9mg/L, and other plant hormone capable of being combined further screens (seeing Figure 1A).
When 2. making up Corticosteroids, DK hormonal composition: 0.1mg/L 2,4-D+2.0mg/L～4.0mg/LKinetin,
Its emergence rate is higher, all is higher than 40%, can further screen (seeing Figure 1B); The NB hormonal composition: 0.1mg/L NAA+7.0mg/L～12.0mg/L 6-BA, emergence rate is higher, all is higher than 40%, can further screen (seeing Figure 1B); The KN hormonal composition: 10.0mg/L Kinetin+1.0mg/L～6.0mg/L NAA, emergence rate all is higher than 40%, can further screen (seeing Figure 1A).
Second takes turns the hormone combination screening, and concrete steps are as follows:
(1) gathered in rear 7 days at FS, fs, CMS, multitude elm B, assorted 37 first rice crops in Yunnan, intercepted respectively FS, fs, CMS, multitude elm B, the assorted 37 first rice crop acrial parts in Yunnan with falling 1～rice straw of 3 node dormancy buds, cleaned;
(2) respectively with the rice straw in the described FS of step (1), fs, CMS, multitude elm B, Yunnan assorted 37, use the hormonal composition of the variable concentrations shown in table 3～table 6 to soak the 48h processing;
(3) evenly be spread out over earthing on the seedling bed with the rice straw of node dormancy bud after step (2) is processed, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling;
(4) treat that the regrowth 3 leaves-4 leaf phase adds up respectively emergence rate, further screening obtains suitable combination and concentration thereof and (sees Table 3～table 6 and Fig. 2～Fig. 5).
Result is found (see figure 2): (0.1mg/L 2 for the DK hormonal composition, 4-D+Kinetin), with 2 of 0.1mg/L, 4-D is quantitative, only have the emergence rate of Kinetin concentration CMS when 2.5mg/L～3.5mg/L to be higher than 40%, remaining emergence rate all is lower than 40%, and the result is all undesirable, casts out the DK hormonal composition so consider.
Result is found (see figure 3): and the NB hormonal composition (0.1mg/L NAA+6-BA), for quantitative, when 6-BA concentration during at 10.0mg/L～12.0mg/L, the emergence rate of 5 kinds of materials is all between 40%～56% take the NAA of 0.1mg/L; When 6-BA concentration is lower than 10.0mg/L, only have the emergence rate of CMS to be higher than 40%.Consider 0.1mg/L NAA+10.0mg/L～12.0mg/L 6-BA and induce emergence rate better, all be higher than 40%, be the suitable for plant hormone combinations proportioning of screening acquisition.
Result is found (see figure 4): and the BN hormonal composition (0.25mg/L 6-BA+NAA), for quantitative, when NAA concentration during at 30.0mg/L～60.0mg/L, the emergence rate of 5 kinds of materials is all between 40%～67% take the 6-BA of 0.25mg/L; When NAA concentration was higher than 60.0mg/L, only having CMS to be 60.0mg/L～120 mg/L and Yunnan assorted 37 in NAA concentration in NAA concentration was that the emergence rate of 60.0mg/L～90mg/L is higher than 40%, and all the other concentration and material emergence rate all are lower than 40%.Consider 0.25mg/L 6-BA+30.0mg/L～60.0mg/L NAA and induce emergence rate better, all be higher than 40%, be the suitable for plant hormone combinations proportioning of screening acquisition.
The BN hormonal composition is the combination according to new proportioning behind the relative preferably single plant hormone NAA of first round screening acquisition and the 6-BA, why not continuing the single hormone of screening is to consider to use single hormone to induce simultaneously the growth efficient of Xin Gen and sleeping bud limited, and use therefore combines them.
Result is found (see figure 5): KN hormonal composition (10mg/L Kinetin+ NAA), and for quantitative, when NAA concentration during at 2.0 mg/L～5.0mg/L, the emergence rate of 5 kinds of materials is between 40%～65% take the Kinetin of 10mg/L; When NAA concentration was lower than 2.0mg/L, the emergence rate in fs, CMS and Yunnan assorted 37 was higher than 40%, and the emergence rate of FS and multitude elm B is lower than 40%; When NAA concentration during greater than 5.0mg/L, the emergence rate in fs, FS, CMS and Yunnan assorted 37 is higher than 40%, and the emergence rate of multitude elm B is lower than 40%.Consider 10mg/L Kinetin+ 2.0 mg/L～5.0mg/L NAA and induce emergence rate better, all be higher than 40%, be the suitable for plant hormone combinations proportioning of screening acquisition.
The assorted 37 5 kinds of materials in comprehensive fs, FS, CMS, multitude elm B and Yunnan are considered:
0.1mg/L NAA+10.0 mg/L～12.0mg/L 6-BA, 0.25mg/L 6-BA+30 mg/L～60mg/L NAA or 10mg/L Kinetin+ 2.0 mg/L～5.0mg/L NAA emergence rate are higher, be suitable plant hormone combination matching, all can induce with the rice straw of sleeping bud and emerge, obtain the good effect of emergence rate between 40%～67%.
Select syzygy 42B(conventional Rice maintainer), multitude elm B(conventional Rice maintainer), the assorted 38(hybrid F in Yunnan
1) the first rice crop acrial part with fall 1～rice straw of 3 node dormancy buds of falling is test material, with hormone solution 0.25mg/L 6-BA+30mg/L NAA immersion treatment 48 hours, and take the seed sprouting seedling in syzygy 42B, multitude elm B, Yunnan assorted 38 as contrast.3 leaves-4 leaf the phase is transplanted to the experimental field, carries out the field production management by the conventional field production management method of paddy rice.Emergence rate, the dry matter of the Sheng of tillering phase, leaf area index and chlorophyll content are also compared in investigation, the spike length when ripe, and Number of kernels etc. are investigated the superiority that hormone induction is regenerated.
Concrete grammar is as described below:
1) gathered in rear 7 days at syzygy 42B, multitude elm B, assorted 37 first rice crops in Yunnan, intercepting syzygy 42B, multitude elm B, the assorted 38 first rice crop acrial parts in Yunnan are cleaned with falling 1～rice straw of 3 node dormancy buds;
2) the described rice straw of step 1) was used respectively solution 0.25mg/L 6-BA+30mg/L NAA immersion treatment 48 hours;
3) will process rear rice straw with the node dormancy bud and evenly be spread out over earthing on the seedling bed, sow under the same conditions syzygy 42B, multitude elm B, assorted 38 seeds in Yunnan simultaneously for contrasting, and all cultivate and manage by paddy rice routine seedbed method of raising seedling;
The rice shoot 3 leaves-4 leaf phase of 4) treating regrowth and seed sprouting is transplanted to the experimental field, and the statistics emergence rate;
5) carry out the field production management by the conventional field production management method of paddy rice after turning green, investigate dry matter, leaf area index and the chlorophyll content of the Sheng phase of tillering, investigation spike length, Number of kernels when ripe.
Result of the test shows (table 7): adopt conventional Rice maintainer and hybrid rice with falling 1～falling the rice straw of 3 node dormancy buds in conjunction with hormone solution immersion treatment, although the regrowth emergence rate is lower than direct emergence rate with seed germination, but every Related Agronomic Characters in each material later stage: dry matter, plant height, spike length, Number of kernels, and leaf area index, the materials variances of chlorophyll content and seed sprouting is not remarkable, show the present invention adopt band fall 1～rice straw of the conventional Rice maintainer of 3 node dormancy buds and hybrid rice of falling all can keep suitable Main Agronomic Characters with conventional seed growing in conjunction with the method for hormone solution-treated, can be normally solid, but the inventive method does not need to carry out hybrid seeding repeatedly, not only avoided the expensive of the paddy rice cross breeding production of hybrid seeds, shorten breeding cycle, can also well keep its hybrid vigour.
Embodiment 4
With fs, CMS, multitude elm B, the assorted 37(hybrid F in Yunnan
1) the first rice crop acrial part with fall 1～rice straw of 3 node dormancy buds of falling is test material, use respectively the hormonal composition immersion treatment, and be contrast with the rice stub in fs, CMS, multitude elm B, Yunnan assorted 37, investigate relatively emergence rate, the plant height of the Sheng of tillering phase, dry matter, leaf area index and chlorophyll content in the process of the test, spike length when ripe, grain husk is spent number etc., investigates the superiority of hormone induction regeneration.
Concrete grammar is as described below:
1) at fs, CMS, multitude elm B, the assorted 37(hybrid F in Yunnan
1) first rice crop gathered in rear 7 days, intercepts respectively fs, CMS, multitude elm B, the assorted 37(hybrid F in Yunnan
1) the first rice crop acrial part is with falling 1～falling the rice straw of 3 node dormancy buds, clean;
2) the described rice straw of step 1) was used respectively solution 0.25mg/L 6-BA+30mg/L NAA immersion treatment 48 hours;
3) will process after with fall 1～rice straw of 3 node dormancy buds of falling evenly is spread out over earthing on the seedling bed, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling;
4) treat that regrowth 3 leaves～4 leaf phases transplant the land for growing field crops, transplanting fs, CMS, multitude elm B, Yunnan assorted 37 under identical field condition leaves down 1～falls the rice stubs of 3 node dormancy buds for contrasting, process and contrast all and carry out the field production management by the conventional field production management method of paddy rice, and the statistics emergence rate.
The investigation test is processed and to impinging upon plant height, dry matter, the leaf area index of the Sheng phase of tillering, investigation spike length, grain husk are spent number when ripe.
Result of the test shows (table 8): utilize emergence rate, leaf area index, the plant height of the regeneration of hormone induction rice straw Nodes sleeping bud all extremely significantly to be better than the rice stub that ratoon rice is directly transplanted; Spike length significantly be longer than the contrast and show significantly sparse, so grain husk spend the number extremely significantly be better than the contrast.
Embodiment 4 shows: for special rice material (such as female sterile, male sterile) and conventional Rice, hybrid rice (hybrid F
1) rice material get with fall 1～fall the rice straw of 3 node dormancy buds in conjunction with HORMONE TREATMENT after emergence rate improved 112.3%～132.9% than the emergence rate of the contrast of direct transplanting rice pile material, dry matter, leaf area index, plant height, spike length, grain husk spend number to increase substantially than contrast, difference is extremely remarkable, has produced unforeseeable technique effect.
Comprehensive embodiment 1～4 result draws, for special rice material (as femalely educate, female sterile, male sterile etc.) and conventional Rice, hybrid rice (hybrid F
1) adopt with fall 1～fall the rice straw of 3 node dormancy buds with HORMONE TREATMENT or need not hormone-treated regeneration efficiency all extremely significantly be better than simulating ratoon rice directly to transplant rice stub regeneration, its emergence rate, leaf area index, plant height, grain husk are spent and are counted, the contrast of Number of kernels geometric ratio increases substantially, difference is extremely remarkable, solved the difficult problem of the difficult breeding of special material, do not need to carry out hybrid seeding repeatedly, expensive, the shortening breeding cycle of the paddy rice cross breeding production of hybrid seeds have not only been avoided, can also well keep its hybrid vigour, produce unforeseeable technique effect.
Claims (3)
1. the paddy rice sleeping bud is bred the method for regeneration plant, may further comprise the steps:
⑴ gathered in rear 7 days at first rice crop, and intercepting paddy rice acrial part is with falling 1～rice straw of 3 node dormancy buds;
⑵ be spread out on the seedling bed and earthing after the described rice straw of step (1) is cleaned, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling, or the described rice straw of step (1) is cleaned rear with plant hormone composition immersion 40～50 hours, be spread out over again on the seedling bed and earthing, grow seedlings and manage by the conventional seedbed of paddy rice method of raising seedling;
⑶ treat that regrowth 3 leaves～4 leaf phases transplant the land for growing field crops, carries out the field production management by the conventional field production management method of paddy rice.
2. paddy rice sleeping bud according to claim 1 is bred the method for regeneration plant, it is characterized in that: the prescription of described plant hormone composition is 0.25mg/L6-BA+30～60mg/L NAA, 0.1mg/L NAA+10.0～12.0mg/L6-BA or 10.0mg/L Kinetin+2.0～5.0mg/L NAA.
3. paddy rice sleeping bud according to claim 1 and 2 is bred the method for regeneration plant, it is characterized in that: the prescription of described plant hormone composition is 0.25mg/L6-BA+30mg/L NAA.
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