CN103060390A - Fuel butanol fermentation method for corn straw hydrolysate - Google Patents
Fuel butanol fermentation method for corn straw hydrolysate Download PDFInfo
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- CN103060390A CN103060390A CN2012103119225A CN201210311922A CN103060390A CN 103060390 A CN103060390 A CN 103060390A CN 2012103119225 A CN2012103119225 A CN 2012103119225A CN 201210311922 A CN201210311922 A CN 201210311922A CN 103060390 A CN103060390 A CN 103060390A
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- butanols
- fuel
- fermentation
- corn stalk
- hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides a fuel butanol fermentation method for corn straw hydrolysate. According to the invention, the corn straw hydrolysate is added into a fermentation medium, and is subjected to fuel butanol fermentation by clostridium acetobutylicum.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to a kind of method of utilizing corn stalk hydrolysis fuel butanols.
Background technology
The energy is the basis of industrial development, and along with the increase of world population and the raising of industrialization degree, the consumption of the energy is also increasing year by year.Because the finiteness of petroleum resources, the novel energy of seeking petroleum replacing has caused the broad interest of scientists.Acetone-butanol is a kind of renewable energy source that can obtain by sugar-fermenting.At present the acetone-butanol produced of biological process is mainly from carbohydrate and amylofermentation, in the face of rapid expansion and the food shortage of world population, will be restricted with the development of grain-production butanols.
Acetone butanol fermentation once was the second largest fermenting process that is only second to zymamsis, and still, since the 1950's, because the development of petroleum industry, acetone butanol fermentation industry is impacted, and moves towards gradually decline.The main component of acetone butanol fermentation product is that (be called for short total solvent, ABE) ratio of its massfraction meets 3: 6: 1 ratio substantially for acetone, butanols and ethanol.Compare with ethanol, butanols (accounting for the acetone butanol fermentation gross product more than 60%) has that energy density and fuel value are high, steam forces down, easily with the advantages such as gasoline mixes, so that the biological butanol fermentation has caused people's very big attention again, become one of focus of biomass energy research and development.Traditional acetone butanol fermentation is take starch, molasses as main raw material, and raw materials cost accounts for more than 60% of acetone butanol fermentation total cost, becomes one of principal element that affects the biological butanol price.Under the double threat of food shortage and energy dilemma, explore cellulose raw material and produce the important component part that the fuel butanols becomes the biomass energy development strategy.
Summary of the invention
The invention provides a kind of method of utilizing corn stalk hydrolysis fuel butanols, the present invention uses clostridium acetobutylicum that it is carried out the fuel butylic fermentation by add corn stalk hydrolysis in fermention medium.
1. a method of utilizing corn stalk hydrolysis fuel butanols is characterized in that using clostridium acetobutylicum that it is carried out the fuel butylic fermentation by add corn stalk hydrolysis in fermention medium.
2. the described corn stalk hydrolysis of step 1 adds 2% dilute sulphuric acid, and solid-liquid ratio is 1:5,121 ℃ of hydrolysis 2 h, Ca (OH)
2Be neutralized to pH value 4.5, stand-by behind the suction filtration.
3. the described fermention medium of step 1 is: 10 mL/L are through the stock solution (KH of 0.22 μ m filtering with microporous membrane
2PO
450 g/L, K
2HPO
450 g/L, ammonium acetate 220 g/L; Para-amino benzoic acid 0.1 g/L, VB 10.1 g/L, VH 0.001 g/L; MgSO47H
2O 20 g/L, MnSO
4H
2O 1g/L, FeSO
47H
2O 1 g/L, NaCl 1 g/L).
4. the described optimum inoculation amount of step 1 is 9%.
5. the initial pH 6.0 of the described the best of step 1.
6. the sugared concentration of the described the best of step 1 is 8%.
7. the described best wheat bran addition of step 1 is 1.5 g/L.
The present invention uses clostridium acetobutylicum that it is carried out the fuel butylic fermentation by add corn stalk hydrolysis in fermention medium, provides important evidence in the hope of the suitability for industrialized production for the fiber butanols.
Description of drawings
Fig. 1 different vaccination amount is on the impact of butylic fermentation.
The different pH values of Fig. 2 are on the impact of butylic fermentation.
Fig. 3 different sugar concentration is on the impact of butylic fermentation.
Fig. 4 different nitrogen sources concentration is on the impact of butylic fermentation.
Embodiment
The following examples elaborate to the present invention, but to the present invention without limits.
The used bacterial strain of the present invention is clostridium acetobutylicum CICC 8016, is preserved in Chinese industrial microbial strains preservation administrative center.
Embodiment 1
The implementation case explanation different vaccination amount for inquiring into the different vaccination amount to the impact of butanols output, uses the different vaccination amount to carry out butylic fermentation to the impact of butanols output, and the result as shown in Figure 1.
As shown in Figure 1, corn mash test tube strains with activation 24 h, be inoculated in the 100 mL fermention mediums by the different vaccination amount, measure the total solvent content in the fermentation liquid behind standing for fermentation 72 h, when inoculum size is 9%, butanols output is the highest, and the output of acetone, ethanol, butanols is respectively 1.32,0.28 and 3.6 g/L, and total solvent is 5.2 g/L.
Embodiment 2
The implementation case illustrates different pH values to the impact of butanols output, for inquiring into different pH values to the impact of butanols output, uses different pH values to carry out butylic fermentation, and the result as shown in Figure 2.
Adjust the initial pH value of fermention medium, fill 100 mL substratum in cubic capacity is the triangular flask of 150mL, by 10% inoculum size, 72 h that ferment, the result as shown in Figure 2.The concentration of product reaches the highest when initial pH value is 6, and butanols output is 4.07 g/L, and total solvent is 6.24 g/L.Therefore choosing pH value 6 is the suitableeest fermentation initial pH value.
The implementation case explanation different sugar concentration for inquiring into different sugar concentration to the impact of butanols output, uses different sugar concentration to butylic fermentation on the impact of butanols output, and the result as shown in Figure 3.
In order to study carbon source concentration to the impact of butylic fermentation, the concentration of corn stalk hydrolysis is adjusted into 20.0 g/L (wherein wood sugar 9.4 g/L), and the glucose that in hydrolyzed solution, adds different concns to make total carbon source concentration be 4%, 6%, 8%, 10% and 12%, the result is as shown in Figure 3.As shown in Figure 3, gradually increase along with carbon source concentration, the output of total solvent and butanols also raises gradually, when carbon source concentration was 8%, total solvent and butanols output were the highest, along with the continuation rising of carbon source concentration, solvent production reduces again thereupon, when above to 12%, the solvent production contrast is low, illustrates that thalline can not the normal growth metabolism under the higher sugar concentration conditions.Compared with the control, increase along with the carbon source addition, the especially residual Xylose Content of residual sugar content increases thereupon, sugar total solvent and sugared butanols transformation efficiency also decrease, when carbon source concentration was 4%, residual sugar content was 3.49 g/L, and when carbon source concentration is 6%, residual sugar content can reach 15.43 g/L (Fig. 3), does not therefore additionally add during the fermentation carbon source.
The implementation case explanation different nitrogen sources concentration for inquiring into different nitrogen sources concentration to the impact of butanols output, uses different nitrogen sources concentration to butylic fermentation on the impact of butanols output, and the result as shown in Figure 4.
Adding wheat bran as organic nitrogen source, is because these organic nitrogen sources may contain the somatomedin of thalline, can promote the metabolism growth of thalline, thereby produce more solvent that in addition, wheat bran is cheap, can make the fermentative production cost.Wheat bran joined in the 100 mL fermention mediums according to different ratios ferment as nitrogenous source, as seen from Figure 4, the output of total solvent and butanols all reached maximum when addition was 1.5 g/L, was respectively 5.79 g/L and 3.64 g/L.
Claims (7)
1. a method of utilizing corn stalk hydrolysis fuel butanols is characterized in that using clostridium acetobutylicum that it is carried out the fuel butylic fermentation by add corn stalk hydrolysis in fermention medium.
2. method according to claim 1 is characterized in that corn stalk hydrolysis, adds 2% dilute sulphuric acid, and solid-liquid ratio is 1:5,121 ℃ of hydrolysis 2 h, Ca (OH)
2Be neutralized to pH value 4.5, stand-by behind the suction filtration.
3. method according to claim 1 is characterized in that, fermention medium is that 10 mL/L are through the stock solution of 0.22 μ m filtering with microporous membrane: KH
2PO
450 g/L, K
2HPO
450 g/L, ammonium acetate 220 g/L; Para-amino benzoic acid 0.1 g/L, VB 10.1 g/L, VH 0.001 g/L; MgSO47H
2O 20 g/L, MnSO
4H
2O 1g/L, FeSO
47H
2O 1 g/L, NaCl 1 g/L.
4. method according to claim 1 is characterized in that, inoculum size is 9%.
5. method according to claim 1 is characterized in that, initial pH 6.0.
6. method according to claim 1 is characterized in that, sugared concentration is 8%.
7. method according to claim 1 is characterized in that, the wheat bran addition is 1.5 g/L.
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| CN2012103119225A CN103060390A (en) | 2012-08-29 | 2012-08-29 | Fuel butanol fermentation method for corn straw hydrolysate |
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| CN2012103119225A CN103060390A (en) | 2012-08-29 | 2012-08-29 | Fuel butanol fermentation method for corn straw hydrolysate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107043793A (en) * | 2017-04-18 | 2017-08-15 | 哈尔滨工业大学 | A kind of method that utilization exogenous electron carrier improves agricultural crop straw hydrolyzate fermentation butyl alcohol yield |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941888A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for fermentation and differential pressure distillation of butanol by stalk dilute acid hydrolyzate |
| CN101942482A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing butanol fermentation culture medium |
| WO2011011796A9 (en) * | 2009-07-24 | 2011-06-23 | The Regents Of The University Of California | Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels |
-
2012
- 2012-08-29 CN CN2012103119225A patent/CN103060390A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941888A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for fermentation and differential pressure distillation of butanol by stalk dilute acid hydrolyzate |
| CN101942482A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing butanol fermentation culture medium |
| WO2011011796A9 (en) * | 2009-07-24 | 2011-06-23 | The Regents Of The University Of California | Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107043793A (en) * | 2017-04-18 | 2017-08-15 | 哈尔滨工业大学 | A kind of method that utilization exogenous electron carrier improves agricultural crop straw hydrolyzate fermentation butyl alcohol yield |
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Application publication date: 20130424 |