CN103060383A - Making method of ceramide and/or glucosylceramide generation accelerator - Google Patents
Making method of ceramide and/or glucosylceramide generation accelerator Download PDFInfo
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Abstract
he invention provides a making method of a ceramide and/or glucosylceramide generation accelerator. Specifically, a Sporobolomyces microorganism is cultivated in a medium containing bean dregs and saccharides, and the ceramide and/or glucosylceramide generation accelerator can be acquired from the supernatant in the medium. According to the making method provided in the invention, specific microorganisms can be utilized, and the by-product bean dregs from the food processing industry and the like can be taken as the main raw material of the medium, so that the ceramide and/or glucosylceramide generation accelerator can be efficiently obtained at a low cost.
Description
Technical field
The present invention relates to utilize specified microorganisms, with the main raw material as substratum such as the by product bean dregs of food-processing industry etc., manufacturing can promote skin to produce ceramide and/or the ceramide of glucose ceramide and/or the method for glucose ceramide production accelerant.
Background technology
The ceramide of one of sphingolipid (Ceramide) is present in the keratoderma, accounts for about 50% of intercellular lipid in the stratum corneum.Sphingolipid (Sphingolipid) is the general name with compound lipid of sphingosine skeleton.Glucose ceramide (Glucosylceramide) is to combine a kind of sphingolipid that glucose forms at ceramide.Temporarily be stored with the form of glucose ceramide or sphingomyelin (Sphingomyelin) by the synthetic ceramide that obtains of epidermic cell; Be discharged to afterwards the extracellular, under the effect of glucose cerebrosidase (Glucocerebrosidase) and sphingomyelinase (Sphingomyelinase), again become ceramide, thereby performance is as the function of intercellular lipid.
All the time, the sphingolipids such as known ceramide, glucose ceramide, galactosyl ceramide have the cuticular maintenance moisture ability of promotion, improve pachylosis texts (patent documentation 1～2).Although skin has the ability that produces ceramide and glucose ceramide, this ability is also insufficient, thereby people have also attempted from the method for external complement ceramide.But the long-term effect of the method can not get approval, and the problem such as existence and stability is low.For example, reported the method (patent documentation 3) of utilizing candida tropicalis (Candida tropicalis) JPCCY0004 strain (NITE P-570) to make the glucose ceramide.But the method is not the method about ceramide and/or glucose ceramide production accelerant, and the method that a large amount of glucose ceramides that exist directly extract in the thalline after only will cultivating; And the ceramide skeleton of the ceramide type that exists in the ceramide skeleton by the synthetic glucose ceramide that obtains of the method and the human body is different, and it has carbon-carbon double bond and have side chain at Liang Chu.Thereby, this glucose ceramide is coated on the human body skin, can not adapt with human body skin and produce the problem of safety in utilization aspect.
And on the other hand, if can strengthen or promote the generation ability of ceramide or the glucose ceramide of skin itself, then need not from external complement ceramide or glucose ceramide, and can improve barrier function and the moisture-keeping functions of skin by promoting skin self generation ceramide or glucose ceramide.Like this, owing to be to produce ceramide or glucose ceramide by skin self, thereby there is not problem with security aspects such as the adaptability of skin are bad.
Therefore, in recent years, quite popular about in keratoderma, studying with the production that promotes the epidermis ceramide to be produced as the material of purpose.As the material that promotes that ceramide produces, that has reported has a ceramide production accelerant (patent documentation 4～6) take the extract of glossy ganoderma, bee pollen (Bee Pollen), Ligusticum wallichii etc. as effective constituent.Yet, because these ceramide production accelerants all are to extract from plant material to obtain, this extractions operation length that expends time in, and utilize the reasons such as possibility is limited, its manufacturing cost height owing to natural resource.
In addition, also reported ceramide synthesis accelerators (patent documentation 7) take the lactic acid bacteria culture that contains nicotinic acid and/or niacinamide or Yeast culture as effective constituent.Yet, can find out from the embodiment of patent documentation 7, do not contain the lactic acid bacteria culture of nicotinic acid and/or niacinamide or the ceramide generation of Yeast culture (comparative example 1～4) and there is no considerable change than control group, thereby this ceramide synthesis accelerators contains in fact nicotinic acid and/or niacinamide as effective constituent, the synthetic facilitation effect of its ceramide is abundant not enough, the leeway that still is improved.And, owing to use nicotinic acid or niacinamide, thereby its manufacturing cost is higher.
In addition, the microorganism of known Sporobolomyces (Sporobolomyces) is accumulated on the industries such as Coenzyme Q10 99.0 useful in thalline.In addition, the microorganism of Sporobolomyces (Sporobolomyces) also receives much concern as the production bacterium of the useful matteies such as carotenoids, VBT.
But, also do not utilize at present the microorganism of Sporobolomyces (Sporobolomyces) to make the report of ceramide and/or glucose ceramide production accelerant.
Patent documentation 1: Japanese kokai publication sho 61-260008 communique
Patent documentation 2: Japanese kokai publication sho 61-271205 communique
Patent documentation 3: TOHKEMY 2010-22217 communique
Patent documentation 4: TOHKEMY 2005-194240 communique
Patent documentation 5: TOHKEMY 2010-70499 communique
Patent documentation 6: TOHKEMY 2010-150237 communique
Patent documentation 7: Japanese kokai publication hei 9-194383 communique
Summary of the invention
For the technical problem that exists in the above-mentioned prior art, the inventor has carried out concentrated research, found that: the microorganism of in the substratum that contains bean dregs and carbohydrate, cultivating Sporobolomyces (Sporobolomyces), particularly cultivate pink shadow yeast (Sporobolomyces roseus), can be low-cost and obtain efficiently ceramide and/or glucose ceramide production accelerant, thus the present invention finished.
The present invention relates to provide the manufacture method of a kind of ceramide and/or glucose ceramide production accelerant, wherein, in the substratum that contains bean dregs and carbohydrate, cultivate the microorganism of Sporobolomyces (Sporobolomyces), obtain from it ceramide and/or glucose ceramide production accelerant in the clear liquid.
The invention still further relates to, provide the fermented product extract of the Sporobolomyces (Sporobolomyces) of bean dregs be used for to promote the purposes of the generation of ceramide and/or glucose ceramide.
The invention still further relates to, provide the fermented product extract of Sporobolomyces (Sporobolomyces) of bean dregs as the purposes of ceramide and/or glucose ceramide production accelerant.
The invention still further relates to, the purposes of fermented product extract in making ceramide and/or glucose ceramide production accelerant of the Sporobolomyces (Sporobolomyces) of bean dregs is provided.
The invention effect
Manufacturing method according to the invention, can utilize specified microorganisms, with the main raw material as substratum such as the by product bean dregs of food-processing industry etc., low-cost and obtain efficiently ceramide and/or glucose ceramide production accelerant, the ability of the generation ceramide that this promotor can Effective Raise skin has/glucose ceramide, thereby the moisture-retaining capacity of Effective Raise skin.
Embodiment
(can be used for the microorganism that ceramide and/or glucose ceramide production accelerant are made)
As the microorganism of the Sporobolomyces (Sporobolomyces) that uses in the present invention, can list at least a kind of microorganism from pink shadow yeast (Sporobolomyces roseus), reddish brown shadow yeast (Sporobolomyces salmonicolor) or dark red shadow yeast (Sporobolomyces ruber) etc. that field of food uses, selecting; The variant that also comprises these microorganisms that obtain according to usual method.These microorganisms are as described below to play a role, and by cultivating in the substratum that contains bean dregs and carbohydrate, thereby can be improved from it the ceramide of epidermic cell and/or the material that the glucose ceramide produces ability in the clear liquid.Wherein, has the viewpoint that higher ceramide produces facilitation effect and glucose ceramide generation facilitation effect, preferred pink shadow yeast (Sporobolomyces roseus) from the product that obtains.
The microorganism of Sporobolomyces involved in the present invention (Sporobolomyces) can be bought from commercial channel such as Chinese common micro-organisms preservation administrative center (CGMCC), Chinese Typical Representative culture collection center (CCTCC), Southern Yangtze University's industrial microorganism resource and information centers (CICIM) and obtain.
(manufacture method of ceramide and/or glucose ceramide production accelerant)
The manufacture method of ceramide of the present invention and/or glucose ceramide production accelerant is cultivated mentioned microorganism by in the substratum that contains bean dregs and carbohydrate, and obtains ceramide and/or glucose ceramide production accelerant in the clear liquid from it.The concrete steps of the method are as follows.
＜substratum 〉
In the present invention, cultivate one kind or two or more in the mentioned microorganism in the preferred liquid medium within.
In the present invention, the main component of liquid nutrient medium is bean dregs and carbohydrate.Also can suitably add peptone, yeast powder.
Because bean dregs and carbohydrate all are the materials that obtains easily, thereby substratum of the present invention has the low advantage of cost.
Wherein, bean dregs refer to, in water, make it fully absorb moisture soybeans soaking after, again through pulverizing, removing by filter water and the material that obtains; The bean product manufacturing that also can use in the food industries squeezing soya-bean milk etc. is remaining bean dregs afterwards.
As carbohydrate, can use separately in glucose, maltose, the sucrose etc. any one, perhaps mix and use.From improving the viewpoint of fermentative production and usability, preferred glucose wherein.
In the present invention, ceramide produces facilitation effect and/or the glucose ceramide produces facilitation effect in order to improve, medium optimization of the present invention is made of bean dregs, glucose and water, further the content of the bean dregs in the preferred culture medium is 5～20w/v%, 8～12w/v% more preferably, and the content of glucose is 0.2～2w/v%, 0.8～1.2w/v% more preferably.
Below, above-mentioned this substratum for the manufacture of ceramide and/or glucose ceramide production accelerant is called " ceramide/glucose ceramide production accelerant is made substratum ".
＜culture condition 〉
The thalline direct inoculation of mentioned microorganism is made in the substratum to ceramide/glucose ceramide production accelerant.As the culture condition for the manufacture of ceramide and/or glucose ceramide production accelerant, from the necessary temperature of growth, the time of optimization Sporobolomyces (Sporobolomyces) microorganism, and the viewpoint that improves ceramide generation facilitation effect and glucose ceramide generation facilitation effect is set out, preferably 20～40 ℃, more preferably under 27～35 ℃ culture temperature, cultivated 1～7 day, the preferred cultivation 48～96 hours more preferably cultivated 68～76 hours.
The recovery of＜ceramide and/or glucose ceramide production accelerant, refining 〉
From the nutrient solution that above-mentioned cultivation obtains, reclaim, make with extra care ceramide and/or glucose ceramide production accelerant, can as required, suitably make up the common operations such as filtration, centrifugation, ion-exchange or adsorption chromatography, solvent extraction, crystallization.Particularly, for example, can remove thalline from nutrient solution by centrifugation, ultrasonication again reclaims the supernatant liquor after centrifugal.Then, the supernatant liquid filtering after will reclaiming is again removed impurity etc., obtains the fermenting bean dregs extract under the vacuum after the drying treatment as ceramide of the present invention and/or glucose ceramide production accelerant.
Shown in following embodiment, by the fermenting bean dregs extract of cultivating Sporobolomyces of the present invention (Sporobolomyces) microorganism and from culture supernatant, obtaining, in normal human's keratinocyte, has the effect of the amount increase that makes ceramide and/or glucose ceramide.
Therefore, by the fermenting bean dregs extract of cultivating Sporobolomyces of the present invention (Sporobolomyces) microorganism and from culture supernatant, obtaining, can be used as ceramide and/or glucose ceramide production accelerant and use, perhaps for the manufacture of ceramide and/or glucose ceramide production accelerant.This ceramide and/or glucose ceramide production accelerant can be used as the ceramide that makes in the stratum corneum and/or the glucose ceramide increases, and recovers or keep the uses such as the barrier function of skin and the pharmaceuticals of moisture-keeping functions, accurate pharmaceuticals, makeup.In addition, this ceramide production accelerant or glucose ceramide production accelerant also can be used as take ceramide produce to promote or the glucose ceramide produce promote as concept, and indicate that as required the accurate pharmaceuticals of this concept, makeup use.
Use form as ceramide of the present invention and/or glucose ceramide production accelerant, can be used as the additive that adds in the culturing cell, perhaps make the external composition for skin such as detergent, makeup, can provide with forms such as astringent, emulsion, gel, frost, ointment, powder, particles according to using method.When the various external composition for skin of preparation, can use individually ceramide of the present invention and/or glucose ceramide production accelerant, or appropriate combination usually uses such as the oiliness composition of cooperation, wetting Agent for Printing Inks, powder, pigment, emulsifying agent, solubilizing agent, UV light absorber, thickening material, effective component, spices, fungi-proofing mould inhibitor, plant milk extract, alcohols in the external composition for skin such as detergent and makeup.
In the epidermic cell that ceramide of the present invention and/or glucose ceramide production accelerant is added to cultivation, promote in the situation of ceramide and/or glucose ceramide, as fermenting bean dregs extract of the present invention (being scaled the drying solid composition), its addition is preferably 0.0001～10w/v%, more preferably 0.001～5w/v%.When addition during greater than 0.0001w/v%, can obtain sufficient facilitation effect; And when addition during less than 10w/v%, less to the pungency of epidermic cell.
When ceramide of the present invention and/or glucose ceramide production accelerant being engaged in external composition for skin and using, thereby from the moisture-retaining capacity of the generation Effective Raise skin of effective promotion ceramide and/or glucose ceramide and be difficult for demonstrating the color of culture and the viewpoint of smell, drying solid composition as the fermenting bean dregs extract, its use level is preferably 0.01～20w/v% of external composition for skin total amount, more preferably 0.1～10w/v%.
Embodiment
Below, the present invention will be described in more detail based on embodiment, but the present invention is not limited thereto.
Embodiment 1
(manufacturing of ceramide and/or glucose ceramide production accelerant)
As the microorganism of making ceramide and/or glucose ceramide production accelerant from bean dregs, use pink shadow yeast (Sporobolomyces roseus) (to buy from Southern Yangtze University, and be preserved in Chinese Universities ' industrial microorganism resource and the CICIM of information center, deposit number CICIM Y0247).
The above-mentioned bacterial strains that will obtain after will cultivating under 30 ℃ in YPD (Yeast Extract Peptone Dextrose Medium) substratum is inoculated in the Erlenmeyer flask (capacity 300ml) that contains ceramide/glucose ceramide production accelerant manufacturing substratum 100ml, 30 ℃ of lower cultivations 72 hours.This substratum contains 10w/v% bean dregs and 1w/v% glucose.Wherein, the preparation of bean dregs was soaked 12 hours by soybean being put into water, made soybean fully absorb moisture, and the soybean that then will absorb moisture is ground broken, after filtering and removing liquid component, residual residue was drained to obtain.
Then, in order from the nutrient solution that obtains, to reclaim ceramide and/or glucose ceramide production accelerant, removed thalline in 10 minutes with the 3000rpm centrifugation, supernatant liquor after the recovery centrifugation, the dehydrated alcohol that adds 2.5 times in the culture supernatant after recovery, obtain the crude product (the present invention's product A) of ceramide of the present invention and/or glucose ceramide production accelerant, be kept under 4 ℃, the ceramide after being used for/glucose ceramide produces and promotes test.
Embodiment 2
As the microorganism of making ceramide/glucose ceramide production accelerant from bean dregs, use pink shadow yeast (Sporobolomyces roseus) (to buy from Southern Yangtze University, and be preserved in Chinese Universities ' industrial microorganism resource and the CICIM of information center, deposit number CICIM Y0193).Adopt the method identical with embodiment 1 to obtain the crude product of ceramide of the present invention and/or glucose ceramide production accelerant, then, use again ultrasonic treatment unit (manufacturing of Branson company) to process this crude product 10 minutes, with 3000rpm centrifugation 30 minutes, reclaim supernatant liquor.With the about 70ml of this supernatant liquor of filter paper filtering, dry this filtered liquid obtains the fermenting bean dregs extract as ceramide of the present invention/glucose ceramide production accelerant to constant weight in Vacuumdrier below 40 ℃ again.
And then, with this fermenting bean dregs extract of 50v/v% dissolve with ethanol, after adjusting its concentration and being 0.1w/v% (the present invention's product B), being kept under 4 ℃, the ceramide after being used for/glucose ceramide produces and promotes test.
Embodiment 3
As the microorganism of making ceramide/glucose ceramide production accelerant from bean dregs, use pink shadow yeast (Sporobolomyces roseus) (to buy from Southern Yangtze University, and be preserved in Chinese Universities ' industrial microorganism resource and the CICIM of information center, deposit number CICIM Y0132).Adopt the method identical with embodiment 1 to obtain the crude product (the present invention's product C) of ceramide of the present invention and/or glucose ceramide production accelerant, then, use again ultrasonic treatment unit (manufacturing of Branson company) to process this crude product 10 minutes, with 3000rpm centrifugation 30 minutes, reclaim supernatant liquor.With the about 70ml of this supernatant liquor of filter paper filtering, dry this filtered liquid obtains the fermenting bean dregs extract as ceramide of the present invention/glucose ceramide production accelerant to constant weight in Vacuumdrier below 40 ℃ again.
And then, with this fermenting bean dregs extract of 50v/v% dissolve with ethanol, after adjusting its concentration and being 1w/v% (the present invention's product D), being kept under 4 ℃, the ceramide after being used for/glucose ceramide produces and promotes test.
(ceramide/glucose ceramide produces and promotes test)
＜keratinocyte (Keratinocytes) is cultivated 〉
At first, get normal people's activation keratinocyte (Cascade Co., Ltd) as primary cell, in 75cm
2In the culturing bottle (the substratum 2ml that contains somatomedin), at 37 ℃, CO
2Concentration is to carry out cell cultures under 5% the condition, is 80～90% until cultivate to cell aggregation density (confluence).Passage cell is to 175cm again
2Continue culturing cell to 80～90% concentration in the square vase.Then with 1 * 10
5Individual cell/ml is passaged in 12 orifice plates (every pore volume is 2ml), continues culturing cell to 80～90% concentration.
Then, change the substratum that does not contain somatomedin, (n=2) adds respectively the aqueous ethanolic solution of the fermenting bean dregs extract that obtains in above-described embodiment 1～3 with the amount in 20 μ l/ holes in above-mentioned 12 orifice plates.In addition, only add in contrast product of 50v/v% ethanol (not adding ceramide/glucose ceramide production accelerant), cultivated 72 hours under the same culture conditions.Cultivate after 72 hours, the supernatant discarded nutrient solution,
Clean cell twice with 2ml PBS (phosphate buffered saline buffer), add again 1ml PBS to plate hole, to test tube, prepare against follow-up ceramide/glucose ceramide and protein analysis with cell harvestor (cell shovel) collecting cell.
＜lipids extraction 〉
Lipids extraction adopts Bligh ﹠amp; The Dyer method is carried out.Particularly, at first, add the solvent of methyl alcohol: chloroform=2.5ml: 1.25ml in the celliferous PBS liquid that above-mentioned recovery obtains, vibration mixed 20 minutes, carried out centrifugation (3000rpm, 5 minutes), thereby obtained supernatant liquor and precipitation.Supernatant A is transferred in the other test tube for ceramide/glucose ceramide quantitative analysis, with the analysis of deposit B for protein content.
＜protein content analysis 〉
For the ceramide in the characterize cells/glucose ceramide amount, carry out the protein content analysis.In the deposit B of above-mentioned recovery, add SDS (sodium lauryl sulphate) solution of 900 μ l and mix, heating made the protein denaturation degraded in 2 hours under 60 ℃ of water-baths, add therein the 2N HCl solution of 100 μ l after the cooling, measure the wherein amount of protein (be designated as Pro, unit is μ g/ml) according to BCAKit (BCA determination of protein concentration test kit) method.
＜ceramide/glucose ceramide component analysis 〉
The solvent that in residual supernatant A, adds PBS: chloroform=1.25ml: 1.25ml; vibration mixed 20 minutes; 3000rpm carried out centrifugation 5 minutes; separate, reclaim the chloroform layer as lower phase; and dry chloroform layer obtained dry product in 40 minutes under 30 ℃, nitrogen protection; the solvent of the chloroform that adds 100 μ l: methyl alcohol=2.5ml: 1.25ml dissolves; and with following thin plate chromatography (TLC) method the amount (be designated as respectively Cer and GlyCer, unit is μ g/ml) of ceramide and glucose ceramide is carried out quantitatively.
Adopt dry TLC plate, take chloroform: methyl alcohol: acetic acid (190: 9: 1 v/v/v) treats that as developing agent carries out the thin plate chromatography developing agent walks to the thin plate top, dries up developing agent with blower; Repeat above step once.Then take chloroform: methyl alcohol: acetone (76: 20: 4 v/v/v) treats that as developing agent carries out the thin plate chromatography developing agent walks to apart from 2.5cm place, thin plate bottom, stops the exhibition layer, dries up with blower.Spray dries up with the copper sulfate phosphoric acid solution, and chromatoplate is positioned over baking colour developing in 7 minutes on 180 ℃ of bake plate.Scanning colour developing TLC plate also carries out ceramide (or glucose ceramide) analysis.
Then, according to following formula (1), income value respectively divided by separately protein content, is obtained Cer/Pro and GlyCer/Pro.Take the Cer/Pro of reference substance and GlyCer/Pro as 1, obtain the relative value of Cer/Pro of the present invention and GlyCer/Pro.And the amount of the protein in the every hole of reference substance is as 100, obtains ceramide in every hole and the amount (being designated as respectively Cer/Well and GlyCer/Well, with respect to the relative quantity of reference substance) of glucose ceramide.In the lump expression in table 1.
Formula (1)
Cer/Pro=ceramide amount (μ g/ml)/protein content (μ g/ml)
GlyCer/Pro=glucose ceramide amount (μ g/ml)/protein content (μ g/ml)
Table 1
As shown in table 1, it is remarkable that the glucose ceramide of the present invention's product that obtain among embodiment 1, the embodiment 3 produces facilitation effect, and the ceramide of the present invention's product that obtain among the embodiment 2 generation facilitation effect is remarkable.
Therefore, confirmed that ceramide of the present invention/glucose ceramide production accelerant has ceramide and produces facilitation effect and/or glucose ceramide generation facilitation effect.
(not having the test of ceramide and glucose ceramide in ceramide of the present invention/glucose ceramide production accelerant)
Without cell cultures, directly adopt the TLC method that the ceramide that obtains in above-described embodiment 1～3/glucose ceramide production accelerant is analyzed, the result shows does not have ceramide and glucose ceramide to exist in the present invention's product.
Show thus, although do not have ceramide or glucose ceramide in the product of the present invention itself, ceramide produces facilitation effect and/or the glucose ceramide produces facilitation effect but have, and can be used as ceramide and/or glucose ceramide production accelerant and uses.
Claims (14)
1. the manufacture method of a ceramide and/or glucose ceramide production accelerant, wherein,
In the substratum that contains bean dregs and carbohydrate, cultivate the microorganism of Sporobolomyces (Sporobolomyces), obtain from it ceramide and/or glucose ceramide production accelerant in the clear liquid.
2. the manufacture method of ceramide as claimed in claim 1 and/or glucose ceramide production accelerant, wherein,
The microorganism of described Sporobolomyces (Sporobolomyces) is pink shadow yeast (Sporobolomyces roseus).
3. the manufacture method of ceramide as claimed in claim 1 and/or glucose ceramide production accelerant, wherein,
Described carbohydrate is glucose.
4. the manufacture method of ceramide as claimed in claim 3 and/or glucose ceramide production accelerant,
Wherein, described substratum is made of bean dregs, glucose and water.
5. the manufacture method of ceramide production accelerant as claimed in claim 4 and/or glucose ceramide production accelerant ceramide and/or glucose ceramide production accelerant, wherein,
The content of bean dregs described in the described substratum is 8～12w/v%, and the content of described carbohydrate is 0.8～1.2w/v%.
6. the manufacture method of ceramide as claimed in claim 1 and/or glucose ceramide production accelerant, wherein,
Described culture temperature is 27～35 ℃.
7. the manufacture method of ceramide as claimed in claim 1 and/or glucose ceramide production accelerant, wherein,
Described incubation time is 1～7 day.
8. the manufacture method of ceramide as claimed in claim 1 and/or glucose ceramide production accelerant, wherein,
To be undertaken by the nutrient solution that described cultivation obtains centrifugal, thereby obtain described supernatant liquor.
9. the manufacture method of ceramide as claimed in claim 8 and/or glucose ceramide production accelerant, wherein,
In the described supernatant liquor of centrifugal rear acquisition, add behind the ethanol its crude product as ceramide and/or glucose ceramide production accelerant.
10. the manufacture method of ceramide as claimed in claim 9 and/or glucose ceramide production accelerant,
Wherein, further with described crude product after ultrasonication, centrifugation, filtration, vacuum drying treatment are made with extra care, obtain ceramide and/or glucose ceramide production accelerant.
11. the fermenting bean dregs extract is used for the purposes of the generation of promotion ceramide and/or glucose ceramide,
Described fermenting bean dregs extract is that the microorganism of cultivating Sporobolomyces (Sporobolomyces) in containing the substratum of bean dregs obtains.
12. the fermenting bean dregs extract is as the purposes of ceramide and/or glucose ceramide production accelerant,
Described fermenting bean dregs extract is that the microorganism of cultivating Sporobolomyces (Sporobolomyces) in containing the substratum of bean dregs obtains.
13. the purposes of fermenting bean dregs extract in making ceramide and/or glucose ceramide production accelerant,
Described fermenting bean dregs extract is that the microorganism of cultivating Sporobolomyces (Sporobolomyces) in containing the substratum of bean dregs obtains.
14. such as the described purposes of any one in the claim 11～13,
Wherein, the microorganism of described Sporobolomyces (Sporobolomyces) is pink shadow yeast (Sporobolomyces roseus).
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110316486.6A CN103060383B (en) | 2011-10-18 | 2011-10-18 | Ceramide and/or glucose ceramide produce the manufacture method of promotor |
| JP2013522070A JP5639713B2 (en) | 2010-11-09 | 2011-11-08 | Method for producing ceramide and / or glucosylceramide production promoter |
| PCT/CN2011/001877 WO2012062043A1 (en) | 2010-11-09 | 2011-11-08 | Method for producing promoter from ceramide and/or glucosylceramide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110316486.6A CN103060383B (en) | 2011-10-18 | 2011-10-18 | Ceramide and/or glucose ceramide produce the manufacture method of promotor |
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| CN103060383A true CN103060383A (en) | 2013-04-24 |
| CN103060383B CN103060383B (en) | 2016-01-20 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11322534A (en) * | 1998-05-14 | 1999-11-24 | Kanebo Ltd | Ceramide synthesis accelerator |
| CN1309718A (en) * | 1998-07-03 | 2001-08-22 | 科斯莫弗姆有限公司 | Improved microbial strains producing sphingoid bases |
| CN101653537A (en) * | 2008-08-18 | 2010-02-24 | 复旦大学附属中山医院 | Ceramide production accelerant |
-
2011
- 2011-10-18 CN CN201110316486.6A patent/CN103060383B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11322534A (en) * | 1998-05-14 | 1999-11-24 | Kanebo Ltd | Ceramide synthesis accelerator |
| CN1309718A (en) * | 1998-07-03 | 2001-08-22 | 科斯莫弗姆有限公司 | Improved microbial strains producing sphingoid bases |
| CN101653537A (en) * | 2008-08-18 | 2010-02-24 | 复旦大学附属中山医院 | Ceramide production accelerant |
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