CN103060296A - Method for extracting trypsin from animal pancreas - Google Patents
Method for extracting trypsin from animal pancreas Download PDFInfo
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- CN103060296A CN103060296A CN2012105980045A CN201210598004A CN103060296A CN 103060296 A CN103060296 A CN 103060296A CN 2012105980045 A CN2012105980045 A CN 2012105980045A CN 201210598004 A CN201210598004 A CN 201210598004A CN 103060296 A CN103060296 A CN 103060296A
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Abstract
The invention discloses a method for extracting trypsin from animal pancreas. According to the technical scheme of the invention, the zymogen in pancreas cells is extracted by using a sulfuric acid solution; subsequently according to the principle of isoelectric precipitation, the pH is adjusted, and the trypsinogen is deposited and precipitated in a classified salting-in and salting out mode by using ammonium sulfate. By utilizing the method, 6g trypsin can be extracted from 1kg of pancreas.
Description
Technical field
The present invention relates to biological technical field, relate in particular to and from animal pancreas, separate tryptic method.
Background technology
Trypsinase Trypsin (Parenzyme) is a kind of of proteolytic enzyme, EC3.4.21.4.In vertebrates, work as digestive ferment.Be synthesized at the precursor trypsinogen of pancreas as enzyme.As the composition of pancreatic juice and secrete, be subjected to enteropeptidase, or tryptic restriction is decomposed into activation trypsinase, be endopeptidase, it can cut off the carboxyl side in Methionin in the polypeptide chain and the arginine residues.It not only plays digestive ferment, and can also limit the precursor of other enzymes such as decomposing chymotrypsinogen, proearboxypeptidase, phosphatide proenzyme, plays activation.Be the strongest proteolytic enzyme of specificity, in the amino acid that determines protein was arranged, it became indispensable instrument.
Trypsin-chymotrypsin and elastoser also have substantial connection aspect structure and the catalyst mechanism, but its specificity is then fully different.A kind of proteolytic ferment that trypsinase system extracts in ox, sheep or pig pancreas.China's drug standard regulation is pressed dry product and is calculated, and must not tire of every 1mg is less than 2500 units.By ox, sheep, Pancreas Sus domestica extract and a kind of endopeptidase, only rupture Methionin or arginic carboxyl participate in the peptide bond that forms.White or beige crystalline powder.Water-soluble, be insoluble to ethanol, glycerine, chloroform and ether.Molecular weight 24000, pI10.5, optimum pH about 7.8～8.5.The irreversible inactivation in pH＞9.0.Ca
2+Enzymic activity there is stabilization; Heavy metal ion, organo phosphorous compounds, DFP, natural trypsin inhibitor have strongly inhibited to its activity.Clinical in anti-inflammation detumescence, industrial for leather manufacture, raw silk processing, food-processing etc.
Trypsinase is proteolytic enzyme, the peptide chain that is selectively consisted of by Methionin or arginic carboxyl in the protein hydrolysate, can digestion dissolve sex change egg matter, without effect, therefore, can make the decomposition, thinning such as purulence, sputum, blood clot to unmodified protein, being easy to drainage gets rid of, the acceleration surface of a wound purifies, and promotes granulation tissue newborn, also has in addition anti-inflammatory effect.Be used for clinically local edema, hemotoncus and the abscess etc. that pyothorax, hemothorax, surgery inflammation, ulcer, traumatic damage, fistula etc. produce.Spraying sucks, and is used for respiratory tract disease.Also can be used for treating venomous snake bite.Also be usually used in the front processing to tissue of animal cell culture.
Thereby tryptic effect is to make intercellular proteolysis make cell dissociation.Different tissues or cell are different to the action-reaction of pancreatin.The activity of pancreatin cell dispersion is also relevant with its concentration, temperature and action time, is 8.0 at pH, when temperature is 37 ℃, and the ability to function of pancreatin solution is the strongest.When using pancreatin, should get hold of concentration, temperature and time, in order to avoid digestion excessively causes cell injury.Because of Ca
2+, Mg
2+Can reduce the activity of pancreatin with serum, protein, not contain Ca so should select during preparation pancreatin solution
2+, Mg
2+BSS, such as D-Hanks liquid.When stopping digestion, availablely contain serum free culture system liquid or pancreatin inhibitor stops pancreatin to the effect of cell.
Extracting trypsinase with existing method is difficult.In July, 2006, A Min pharmaceutical Co. Ltd in Shanghai disclosed a kind of preparation method (200610023582.0) of Trypsin-chymotrypsin, it adopts low temperature acid to carry, gradient is saltoutd, the Product Process of frozen water dialysis, affinity chromatography, alcohol chromatography, obtains Trypsin-chymotrypsin.The loyal benevolence of in January, 2011 horse etc. has been applied for a kind of method (200910170682.X) of extracting Trypsin-chymotrypsin, and it has adopted CaCl
2Activate (the NH that adds saturation ratio when Trypsin-chymotrypsin is former to become Trypsin-chymotrypsin
4)
2SO
4, make it generate calcium sulfate precipitation absorption Trypsin-chymotrypsin, through wash-out, saltout, lyophilized powder that ultrafiltration and freeze-drying obtain the Trypsin-chymotrypsin of white.Product is the mixture of Trypsin-chymotrypsin, does not have simple extraction trypsinase.The present invention has reduced cost, and trypsinase can be extracted.
Summary of the invention
The objective of the invention is from the pancreas of animal, to extract trypsinase, realize the separation of Trypsin-chymotrypsin.
Technical scheme of the present invention is: utilize sulphuric acid soln that the proenzyme that contains in the pancreatic cell is extracted, then according to the principle of isoelectric precipitation, regulate pH, ammonium sulfate classification salt is molten, saltout Precipitations such as trypsinogens, comprises the steps:
(1) abstraction process: fresh pancreas is rubbed into the pancreas slurry, weigh.0.1～0.2mol/L sulphuric acid soln by every kilogram of pancreas slurry adding 2～3L flooded 20～28 hours, macerate is put filter bag filter, and discarded filter residue after being filtered dry, and left and took filtrate;
(2) the molten operation of salt: add ammonium sulfate in the steeping fluid, reach 30～50% saturation ratios, left standstill 10～16 hours, filter, leave and take filtrate;
(3) salting-out procedures: add ammonium sulfate in the filtrate, reach 50～80% saturation ratios, left standstill 10～16 hours, filter, leave and take filter cake;
(4) every gram filter cake adds the water dissolution of 2～5ml, repeats above-mentioned (2), (3) operation;
(5) then every gram filter cake adds the saturated ammonium sulphate solution of 0.1～1ml with the water dissolution of 1～3ml in every gram filter cake, regulates pH to 4.0～6.0;
(6) Crystallization Procedure: solution left standstill 24～72 hours in 20～30 ℃ of temperature, and needle-like Chymotrypsin crystallization is arranged; Centrifugal, regulate pH to 2.0～5.0, add 300～400g solid ammonium sulfate in every 1000ml solution, reach 50～80% saturation ratios, left standstill suction filtration 12～16 hours;
(7) drying process: taking precipitate, drying namely gets the trypsinase crude product.
Embodiment
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1
The described dilute acid soln that utilizes extracts the proenzyme that contains in the pancreatic cell, then according to the principle of isoelectric precipitation, regulate pH and remove a large amount of acid foreign proteins and non-albumen impurity with precipitation, again with grade ammonium sulfate salting-out with Precipitations such as trypsinogens, comprise the steps:
1. abstraction process:
1.1 fresh pancreas is removed adipose connective tissue etc. with tweezers and scissors, puts and rubs in the pulverizer, 15Kg weighs;
Put in the plastic tank 1.2 rub thing, 30L flooded 24 hours with the 0.125mol/L sulphuric acid soln, interim per hour the stirring 1 time of dipping;
Filter 1.3 macerate is put filter bag, filter residue flooded 1 hour with 0.125mol/L sulphuric acid soln 15L again, discarded filter residue after being filtered dry;
2. molten, the salting-out procedures of salt:
2.1 twice steeping fluid merged, and volume is 44.9L, puts in the plastic tank, adds solid ammonium sulfate 242g in every 1000ml solution, reaches 40% saturation ratio, adds altogether the 10.866Kg solid ammonium sulfate, leaves standstill 12 hours;
2.2 filter, leave and take filtered liquid, filtrate volume is 44L, solid discards;
2.3 in every 1000ml clear liquid, add solid ammonium sulfate 205g, reach 70% saturation ratio, share ammonium sulfate 9.020kg, left standstill 12 hours;
2.4 filter, leave and take filter cake, the 269.2g that weighs abandons filtrate;
2.5 use the 807.6ml water dissolution, again by 2.1,2.2,2.3,2.4 step gradation reinforcing body ammonium sulfate nearly 40% and 70% saturation ratio precipitation;
2.670% saturation ratio throw out, the 178.5g that weighs uses the 267.8ml water dissolution, adds 89.3ml saturated ammonium sulphate solution, and regulates pH to 5.00 with the 5mol/L sodium hydroxide solution;
2.7 with in 25 ℃ of thermostat containers of solution left standstill 48 hours, the Chymotrypsin crude product crystallization of needle-like is arranged;
2.8 filter, mother liquor is that 328.4ml adds ammonium sulfate 100.8g with 0.25mol/L sulphur acid for adjusting pH to 3.0, its volume, leaves standstill 12 hours;
3. drying process:
Taking precipitate is put the vacuum drying oven inner drying, namely gets the trypsinase crude product, the 91.8g that weighs, and namely every kilogram of pancreas can get 6.12 gram trypsinase.
Embodiment 2
1. abstraction process:
1.1 fresh pancreas is removed adipose connective tissue etc. with tweezers and scissors, puts and rubs in the pulverizer, 10Kg weighs;
Put in the plastic tank 1.2 rub thing, 25L flooded 24 hours with the 0.150mol/L sulphuric acid soln, interim per hour the stirring 1 time of dipping;
Filter 1.3 macerate is put filter bag, filter residue flooded 1 hour with 0.150mol/L sulphuric acid soln 12.5L again, discarded filter residue after being filtered dry;
2. molten, the salting-out procedures of salt:
2.1 twice steeping fluid merged, and volume is 37.4L, puts in the plastic tank, adds solid ammonium sulfate 225g in every 1000ml solution, reaches 35% saturation ratio, power 8.415Kg solid ammonium sulfate left standstill 12 hours altogether;
2.2 filter, leave and take filtered liquid, filtrate volume is 36.8L, solid discards;
2.3 in every 1000ml clear liquid, add solid ammonium sulfate 186g, reach 65% saturation ratio, share ammonium sulfate 6.845kg, left standstill 12 hours;
2.4 filter, leave and take filter cake, the 180.2g that weighs abandons filtrate;
2.5 use the 720.8ml water dissolution, again by 2.1,2.2,2.3,2.4 step gradation reinforcing body ammonium sulfate nearly 35% and 65% saturation ratio precipitation;
2.6 get 65% saturation ratio throw out, the 90.5g that weighs uses the 181ml water dissolution, adds 54.3ml saturated ammonium sulphate solution, and regulates pH to 5.10 with the 5mol/L sodium hydroxide solution;
2.7 with in 25 ℃ of thermostat containers of solution left standstill 48 hours, the Chymotrypsin crude product crystallization of needle-like is arranged;
2.8 filter, mother liquor is that 234.7ml adds ammonium sulfate 82.1g with 0.25mol/L sulphur acid for adjusting pH to 2.9, its volume, leaves standstill 15 hours;
3. drying process:
Taking precipitate is put the vacuum drying oven inner drying, namely gets the trypsinase crude product, the 60.9g that weighs, and namely every kilogram of pancreas can get 6.09 gram trypsinase
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (6)
1. one kind is extracted tryptic method from animal pancreas, it is characterized in that the method comprises the steps:
(1) abstraction process: the pancreas of animal is rubbed into the pancreas slurry, with the sulphuric acid soln dipping, filter, leave and take filtrate;
(2) the molten operation of salt: add ammonium sulfate in the filtrate, make the saturation ratio of ammonium sulfate reach 30～50%, leave standstill, filter, leave and take filtrate;
(3) salting-out procedures: add ammonium sulfate in the filtrate, make the saturation ratio of ammonium sulfate reach 50～80%, leave standstill, filter, leave and take filter cake;
(4) the gained filter cake is dissolved in water, and adds saturated ammonium sulphate solution, regulates pH value to 4.0～5.5;
(5) Crystallization Procedure: solution left standstill is to needle-like Chymotrypsin crystallization is arranged; Centrifugal, regulate pH value to 2.0～5.0, in solution, add solid ammonium sulfate, suction filtration;
(6) drying process: taking precipitate, drying namely gets trypsinase.
2. method according to claim 1, it is characterized in that: in the step (1), the concentration of sulphuric acid soln is 0.1～0.2mol/L; The pancreas slurry is 1: 2～1: 3 with the weight ratio of sulphuric acid soln; Dipping time is 20～28 hours.
3. method according to claim 1, it is characterized in that: in the step (4), the weight ratio of water and filter cake is 1: 1～3: 1; Regulate pH value to 4.0～6.0; The saturated ammonium sulphate solution that adds and the weight ratio of filter cake are 1: 10～1: 1.
4. method according to claim 1 is characterized in that: in the step (5), it is 20～30 ℃ that envrionment temperature is left standstill in dissolving; Regulate pH value to 2.0～5.0; The weight that adds solid ammonium sulfate in every 1000ml solution is 300～400g.
5. method according to claim 1, it is characterized in that: described animal pancreas is fresh Pancreas Bovis seu Bubali, fresh Pancreas Sus domestica.
6. method according to claim 1 is characterized in that: in step (4) before, also comprises gained filter cake water dissolution, and the operation steps of repeating step (2) and step (3), the weight ratio of water and filter cake is 2: 1～5: 1.
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| CN201210598004.5A CN103060296B (en) | 2012-12-30 | 2012-12-30 | Method for extracting trypsin from animal pancreas |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103725666A (en) * | 2013-11-28 | 2014-04-16 | 青岛康原药业有限公司 | Production technology for trypsin competitive product |
| CN103805585A (en) * | 2013-11-28 | 2014-05-21 | 青岛康原药业有限公司 | Method for extracting chymotrypsin from animal pancreas |
| CN104593345A (en) * | 2014-12-25 | 2015-05-06 | 青岛康原药业有限公司 | Method for separation and purification of trypsin and pharmaceutical composition containing trypsin |
| CN104762284A (en) * | 2015-03-09 | 2015-07-08 | 上海上药第一生化药业有限公司 | A preparing method of high-purity trypsin |
| JP2017500885A (en) * | 2013-11-05 | 2017-01-12 | アラガン ファーマシューティカルズ インターナショナル リミテッド | High potency pancreatin pharmaceutical composition |
| US9976171B2 (en) | 2011-08-08 | 2018-05-22 | Allergan Pharmaceuticals International Limited | Method for dissolution testing of solid compositions containing digestive enzymes |
| US10184121B2 (en) | 2013-06-28 | 2019-01-22 | Allergan Pharmaceuticals International Limited | Methods for removing viral contaminants from pancreatic extracts |
| US10206882B2 (en) | 2007-02-20 | 2019-02-19 | Allergan Pharmaceuticals International Limited | Stable digestive enzyme compositions |
| US10993996B2 (en) | 2013-08-09 | 2021-05-04 | Allergan Pharmaceuticals International Limited | Digestive enzyme composition suitable for enteral administration |
| US11364205B2 (en) | 2010-10-01 | 2022-06-21 | Societe Des Produits Nestle S.A. | Stable low digestive enzyme content formulation |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10206882B2 (en) | 2007-02-20 | 2019-02-19 | Allergan Pharmaceuticals International Limited | Stable digestive enzyme compositions |
| US11364205B2 (en) | 2010-10-01 | 2022-06-21 | Societe Des Produits Nestle S.A. | Stable low digestive enzyme content formulation |
| US9976171B2 (en) | 2011-08-08 | 2018-05-22 | Allergan Pharmaceuticals International Limited | Method for dissolution testing of solid compositions containing digestive enzymes |
| US10184121B2 (en) | 2013-06-28 | 2019-01-22 | Allergan Pharmaceuticals International Limited | Methods for removing viral contaminants from pancreatic extracts |
| US10993996B2 (en) | 2013-08-09 | 2021-05-04 | Allergan Pharmaceuticals International Limited | Digestive enzyme composition suitable for enteral administration |
| CN106659773A (en) * | 2013-11-05 | 2017-05-10 | 阿勒根制药国际有限公司 | High potency pancreatin pharmaceutical compositions |
| EP3003360A4 (en) * | 2013-11-05 | 2017-04-26 | Aptalis Pharma Limited | High potency pancreatin pharmaceutical compositions |
| JP2017500885A (en) * | 2013-11-05 | 2017-01-12 | アラガン ファーマシューティカルズ インターナショナル リミテッド | High potency pancreatin pharmaceutical composition |
| EP3613429A1 (en) * | 2013-11-05 | 2020-02-26 | Allergan Pharmaceuticals International Limited | High potency pancreatin pharmaceutical compositions |
| IL274583A (en) * | 2013-11-05 | 2020-06-30 | Pharmaceuticals International Limited Allergan | High potency pancreatin pharmaceutical compositions |
| CN103725666A (en) * | 2013-11-28 | 2014-04-16 | 青岛康原药业有限公司 | Production technology for trypsin competitive product |
| CN103805585A (en) * | 2013-11-28 | 2014-05-21 | 青岛康原药业有限公司 | Method for extracting chymotrypsin from animal pancreas |
| CN104593345A (en) * | 2014-12-25 | 2015-05-06 | 青岛康原药业有限公司 | Method for separation and purification of trypsin and pharmaceutical composition containing trypsin |
| CN104762284B (en) * | 2015-03-09 | 2017-11-24 | 上海上药第一生化药业有限公司 | A kind of preparation method of high-purity trypsase |
| CN104762284A (en) * | 2015-03-09 | 2015-07-08 | 上海上药第一生化药业有限公司 | A preparing method of high-purity trypsin |
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| CN103060296B (en) | 2014-05-21 |
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Address after: 266100 Zhuzhou Road, Laoshan District, Shandong, No. 97, No. Patentee after: Qingdao Jiulong biological medicine group Co., Ltd. Address before: 266100 Zhuzhou Road, Laoshan District, Shandong, No. 97, No. Patentee before: Qingdao Jiulong Bio-Pharmaceutical Co., Ltd. |
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Effective date of registration: 20190412 Address after: 266100 No. 108-1 Zhuzhou Road, Laoshan District, Qingdao City, Shandong Province Patentee after: Qingdao China Pharmaceutical Co., Ltd. Address before: 266100 No. 97 Zhuzhou Road, Laoshan District, Qingdao City, Shandong Province Patentee before: Qingdao Jiulong biological medicine group Co., Ltd. |