CN103054554B - Optical imaging device capable of deep scanning along axial direction and method and application thereof - Google Patents
Optical imaging device capable of deep scanning along axial direction and method and application thereof Download PDFInfo
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- CN103054554B CN103054554B CN201210591214.1A CN201210591214A CN103054554B CN 103054554 B CN103054554 B CN 103054554B CN 201210591214 A CN201210591214 A CN 201210591214A CN 103054554 B CN103054554 B CN 103054554B
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- 238000012634 optical imaging Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims description 11
- 239000000523 sample Substances 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 238000003384 imaging method Methods 0.000 claims abstract description 21
- 239000000835 fiber Substances 0.000 claims description 19
- 239000013307 optical fiber Substances 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 6
- 239000005357 flat glass Substances 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 description 5
- 238000001574 biopsy Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000005081 epithelial layer Anatomy 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000879 optical micrograph Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
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Abstract
The invention discloses an optical imaging device capable of deep scanning along axial direction and application thereof. The optical imaging device capable of deep scanning along axial direction comprises following members in sequence: a light source, a collimating lens, a liquid varifocus lens and an imaging lens. The collimating lens changes divergent beams of the light source into parallel beams. The liquid varifocus lens focuses the parallel beams and controls axial position of focusing spots through a driving voltage. The imaging lens images the spots which are focused through the liquid varifocus lens in the sample so as to scan in an axial direction. Compared with the existing miniature probe capable of deep scanning, the optical imaging device capable of deep scanning along axial direction can allow the imaged spots to be scanned axially in the sample through a way of changing the focal length of the liquid varifocus lens under the control of voltage without extra mechanical parts. The optical imaging device capable of deep scanning along axial direction is simple in structure so as to easily miniaturize the probe. Besides, controlling the axial deep scanning becomes easier, thereby allowing the probe to easily combine with the existing rigid endoscope into use so as to be suitable for tissue to perform confocal micro-imaging living analysis.
Description
Technical field
The present invention relates to optical imaging field, particularly a kind of optical imaging device, method and application thereof of carrying out vertically depth scan.
Background technology
In modern optical microphotograph imaging system, particularly confocal microscopic image system, system usually needs to obtain by 3-D scanning the three-dimensional spatial information of sample, thereby can more comprehensively analyze sample.This technology is even more important in the earlier detection of cancer, because it can Real-time Obtaining biological tissue epithelial layer cellularity information, can provide reliable criterion to the diagnosis of cancer early lesion and classification, avoid being undertaken by biopsy the loaded down with trivial details step of pathological analysis, alleviate misery and financial burden that repeatedly biopsy brings to patient.
3-D scanning is made up of horizontal two-dimensional scan and axial one-dimensional scanning conventionally.The technology of many two dimensions (x-y) scanning technique can be referring to United States Patent (USP) WO00/75712, and axial (z) or depth scan mode mainly comprise: utilize moving axially of hydraulic-driven optical imaging system, utilize micromachine to drive moving axially of optical imaging system, thereby the thermal deformation that utilizes shape memory metal drives optical fiber input end to move the axial scan of realizing optical imaging system, utilize microelectron-mechanical (MEMS) device to control distorting lens to realize the axial scan etc. of luminous point.But these axial scanning methods all need the mechanical part of a little particular design, exist the size of scanning optical imaging device larger, or mechanical devices processed complex, high in cost of production defect.
Because conventional medical detecting method often need to obtain by endoscope the epithelial layer information of tissue, and the size that the biopsy channel size of endoscope will restriction optical imaging system.Therefore, development is a kind of simple in structure, and it is convenient to control, and undersized axial scan optical imaging system is very necessary.
Summary of the invention
For addressing the above problem, the invention provides a kind of optical imaging device that carries out vertically depth scan, it does not need extra mechanical part, simple in structure, volume size is less, and the combination of easy and existing endoscope, for scope detects the microstructure information that tissue is provided.
Another object of the present invention is to provide a kind of optical imaging method that carries out vertically depth scan.
The object of the present invention is achieved like this: a kind of optical imaging device that carries out vertically depth scan, it is characterized in that comprising be arranged in order with lower component: lighting source; The divergent beams of lighting source are become to the collimating lens of collimated light beam; By above-mentioned collimated light beam focusing and by the liquid zoom lens of driving voltage control focal spot axial location; The luminous point focusing on through liquid zoom lens is imaged on to sample inside and carries out the imaging len of axial scan.
Described optical imaging device adopts confocal microscopic image pattern, receives backscatter signal or the fluorescence signal in the illuminated region of sample using optical fiber as lighting source simultaneously.
Described optical fiber is single-mode fiber.
The size of the driving voltage of described liquid zoom lens is by the depth scan distance calibration of determining, obtain definite axial scan luminous point by inputting corresponding driving voltage, this axial scan luminous point is positioned at the axial or depth direction of relative not making alive time point initial position.
Described axial scan scope is at 100-500 micron.
Described collimating lens, liquid zoom lens, imaging len are encapsulated in probe, one end of probe is provided with respectively the independently circuit control signal transfer wire passage of optic fibre input end mouth and liquid zoom lens, light path path separates with transfer wire, and the other end is sealed by light window glass.
Described probe diameter is less than or equal to 3 millimeters.
One end that described probe is provided with optic fibre input end mouth is provided with optical-fiber two-dimensional scan module.
An optical imaging method that carries out vertically depth scan, is characterized in that: introduce illumination light by optical fiber, optical fiber receives backscatter signal or the fluorescence signal in the illuminated region of sample simultaneously as lighting source; Illumination is strolled by collimating lens and is become collimated light beam; This light beam can be polymerized to luminous point again by liquid zoom lens, and the voltage being added on liquid zoom lens by change moves the luminous point of assembling vertically; This luminous point is focused at the inner focus that forms of sample by imaging len; Backscatter signal or the fluorescence signal of focus field of illumination return along original optical path, are finally received by optical fiber.
A kind of endoscope that comprises above-mentioned optical imaging device.
The relatively existing miniature probe that carries out depth scan, the present invention changes the focal length of liquid zoom lens by Control of Voltage, can be embodied as the axial scan of picture luminous point in sample, do not need extra mechanical part, simple in structure, be more prone to make the miniaturization of popping one's head in, and axial depth scan control is also simpler, thereby probe is more easily combined with existing hard scope, is suitable for the confocal microscopic image in-vivo analysis of tissue.
Brief description of the drawings:
Fig. 1 is cross-sectional view of the present invention;
Fig. 2 is the structural representation of left side shown in Fig. 1.
Detailed description of the invention
Describe the present invention in detail below in conjunction with drawings and Examples, but the present invention is not limited to this specific examples.
As shown in Figure 1, the present invention is a kind of optical imaging device that carries out vertically depth scan, comprises the lighting source, collimating lens 2, liquid zoom lens 3 and the imaging len 4 that are arranged in order.
Optics comprises collimating lens 2, and liquid zoom lens 3 and imaging len 4, introduce illumination light by optical fiber.Optical imaging device adopts confocal microscopic image pattern, and optical fiber receives backscatter signal or the fluorescence signal in the illuminated region of sample simultaneously as lighting source.Preferably, optical fiber is single-mode fiber 8.The light of exporting from single-mode fiber 8 is exactly the lighting source of axial scan optical imagery, and single-mode fiber 8 is also equivalent to the pin hole of confocal microscopic image system simultaneously, effectively filters near the backward scattered veiling glare of optical imaging system focal point.The approximate some lighting source of end face of single-mode fiber 8, it and interior one of sample are assembled luminous point (example convergent point 6,7 as shown in Figure 1) conjugation.The little luminous point of gaussian shaped profile from single-mode fiber 8 becomes collimated light beam by collimating lens 2, and this light beam can be polymerized to luminous point again by liquid zoom lens 3, and this luminous point is focused at sample inside by imaging len 4, as convergent point 6,7.Backscatter signal or fluorescence signal from these convergence luminous point 6,7 field of illuminations return along original optical path, the detector of input probe outside after finally being received by single-mode fiber 8, this is the confocal microscopic image system that a kind of typical illuminator is identical with the system of accepting.Like this, can make light that the single-mode fiber 8 of sound end face exports through optics post-concentration different depth in sample by Control of Voltage.
Liquid zoom lens 3 is automatically controlled, and the voltage being added on liquid zoom lens 3 by change changes the radius of curvature on two liquid level borders, thereby changes the focal length of liquid zoom lens 3.When collimated light beam sees through liquid zoom lens 3, assembling luminous point just will be moved vertically, as shown in Figure 1, under different voltage, make the collimated light beam of self-focus lens 2 will be along the different path transmissions of light 10 and light 11, thereby the luminous point of assembling through liquid zoom lens 3 is moved vertically, after the effect that moves through imaging len 4 of this luminous point, realizing illumination spot different depth in sample focuses on, form different convergent point 6,7, realize the axial depth scanning of luminous point.
Optical imaging device of the present invention is by the driving voltage size of the depth scan distance calibration liquid zoom lens 3 of determining, can obtain one to one definite axial scan luminous point by inputting corresponding driving voltage, this axial scan luminous point is positioned at the axial or depth direction of relative not making alive time point initial position.The zooming range 12 of zoom lens 3 depends on the voltage swing being carried on liquid zoom lens 3.Zooming range, in conjunction with the axial enlargement ratio of imaging len 4, ensures that luminous point is less than or equal to the depth scan scope of 500 microns in sample interior realization.
Collimating lens 2 and imaging len 4 are actually the battery of lens that formed by more than two or two lens combinations respectively, can ensure like this spatial resolution and the aberration that reduces imaging system of optical imaging device.The combination of liquid zoom lens 3 and collimating lens 2 makes on the focal plane of liquid zoom lens 3, to obtain the very little luminous point of distortion from the two-dimensional scan luminous point of optical-fiber two-dimensional scan module 1.The combination of liquid zoom lens 3 and imaging len 4 make illumination spot vertically moving range be less than or equal to 500 microns, preferred axial scan scope is at 100-500 micron, and the distortion of this illumination spot within the scope of whole axial scan is very little.
Described collimating lens 2, liquid zoom lens 3, imaging len 4 is encapsulated in the miniature probe 15 of rustless steel parcel.As shown in Figure 2, one end of probe 15 is provided with optical-fiber two-dimensional scan module 1, the optic fibre input end mouth of fixing single-mode fiber 8, the circuit control signal transfer wire passage 9 of liquid zoom lens 3, light path path is separated with transfer wire 13, avoid the running voltage of transmission to impact the safety of popping one's head in; The other end is sealed so that clean and sterilize by light window glass 5.The diameter of miniature probe 15 can be less than or equal to 3mm, can be placed in the instrument channel of hard hysteroscope.The scanning technique of optical-fiber two-dimensional scan module 1 can be referring to prior art, be not described in detail at this, its the outgoing luminous point that can realize single-mode fiber 8 is scanning perpendicular to the two dimensional surface of Optical devices optical axis, axial scan optical imaging device in binding probe 15, can carry out three-dimensional imaging to sample.Relation between axial scan position and the control voltage of liquid zoom lens of probe 15 can be demarcated, thereby can determine the depth location that sample message is concrete, avoid often needing feedback signal control circuit accurately to determine the depth location of scanning element in other depth scan methods, greatly simplified structure and the cost of scanning means.The great advantage of this three-dimensional imaging probe is exactly simple in structure, easily miniaturization, and scan control is simple.
By above-mentioned optical imaging device and the combination of existing endoscope, can obtain the three dimensional structure information of sample, for scope detects the microstructure information that tissue is provided.Due to small volume, therefore can pass through the biopsy channel of hard scope, be suitable for the confocal microscopic image in-vivo analysis of tissue.
Claims (8)
1. carry out vertically an optical imaging device for depth scan, it is characterized in that comprising be arranged in order with lower component:
～lighting source;
～divergent beams of lighting source are become to the collimating lens of collimated light beam;
～by above-mentioned collimated light beam focusing and by the liquid zoom lens of driving voltage control focal spot axial location;
～luminous point focusing on through liquid zoom lens is imaged on to sample inside and carries out the imaging len of axial scan;
Described optical imaging device adopts confocal microscopic image pattern, receives backscatter signal or the fluorescence signal in the illuminated region of sample using optical fiber as lighting source simultaneously, and described optical fiber is single-mode fiber.
2. the optical imaging device that carries out vertically depth scan according to claim 1, it is characterized in that: the size of the driving voltage of described liquid zoom lens is by the depth scan distance calibration of determining, obtain definite axial scan luminous point by inputting corresponding driving voltage, this axial scan luminous point is positioned at the axial or depth direction of relative not making alive time point initial position.
3. the optical imaging device that carries out vertically depth scan according to claim 1, is characterized in that: described axial scan scope is at 100-500 micron.
4. the optical imaging device that carries out vertically depth scan according to claim 1, it is characterized in that: described collimating lens, liquid zoom lens, imaging len are encapsulated in probe, one end of probe is provided with respectively the independently circuit control signal transfer wire passage of optic fibre input end mouth and liquid zoom lens, path channels separates with transfer wire, and the other end is sealed by light window glass.
5. the optical imaging device that carries out vertically depth scan according to claim 4, is characterized in that: described probe diameter is less than or equal to 3 millimeters.
6. the optical imaging device that carries out vertically depth scan according to claim 4, is characterized in that: one end that described probe is provided with optic fibre input end mouth is provided with optical-fiber two-dimensional scan module.
7. an optical imaging method that carries out vertically depth scan, is characterized in that: introduce illumination light by single-mode fiber, single-mode fiber receives backscatter signal or the fluorescence signal in the illuminated region of sample simultaneously as lighting source; Illumination light becomes collimated light beam by collimating lens; This light beam can be polymerized to luminous point again by liquid zoom lens, and the voltage being added on liquid zoom lens by change moves the luminous point of assembling vertically; This luminous point is focused at the inner focus that forms of sample by imaging len; Backscatter signal or the fluorescence signal of focus field of illumination return along original optical path, are finally received by single-mode fiber.
8. one kind comprises in claim 1-6 the endoscope of optical imaging device described in arbitrary claim.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108387186A (en) * | 2018-03-22 | 2018-08-10 | 深圳技术大学（筹） | A kind of three-dimensional scanner based on digital micro-mirror device coding |
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| CN110062603B (en) * | 2016-12-02 | 2023-06-16 | 密歇根大学董事会 | Multiphoton endoscopic microscope for vertical cross-section imaging |
| CN109718476A (en) * | 2018-12-28 | 2019-05-07 | 中国科学院苏州生物医学工程技术研究所 | 3-D scanning two-photon stimulating system and its stimulating method |
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| CN112998647B (en) * | 2019-12-20 | 2024-05-28 | 财团法人金属工业研究发展中心 | Electrowetting control optical scanning probe |
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Effective date of registration: 20150930 Address after: 526061 Guangdong Province, Zhaoqing city Duanzhou District Donggang Zhaoqing University Patentee after: ZHAOQING University Address before: 526061 Guangdong Province, Zhaoqing city Duanzhou District Donggang Zhaoqing University Patentee before: Chen Yingjun Patentee before: Liu Yong |
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