CN103044563A - Method for extracting hericium erinaceus polysaccharide - Google Patents
Method for extracting hericium erinaceus polysaccharide Download PDFInfo
- Publication number
- CN103044563A CN103044563A CN 201210571817 CN201210571817A CN103044563A CN 103044563 A CN103044563 A CN 103044563A CN 201210571817 CN201210571817 CN 201210571817 CN 201210571817 A CN201210571817 A CN 201210571817A CN 103044563 A CN103044563 A CN 103044563A
- Authority
- CN
- China
- Prior art keywords
- extraction
- hericium erinaceus
- hot water
- polysaccharide
- weight ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Extraction Or Liquid Replacement (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting hericium erinaceus polysaccharide. The hericium erinaceus polysaccharide is extracted by three methods of hot water extraction, ultrasonic extraction and microwave extraction. The method is quick and efficient and combines three extraction modes. Microwave can penetrate an extraction medium and directly acts on a material, so that the material temperature is increased quickly, the solubility of the target substance in the medium is increased, and the quality is stable. Microwave extraction heating speed is high, the heated time of the material is short, and extraction operation can be finished at low temperature, so the functional components of the polysaccharide can be effectively protected, and the advantages of materials saving, energy saving and environmental friendliness are achieved. Hot water extraction and ultrasonic extraction are the traditional extraction modes, operating parameters are optimized, the speed and the yield of the extraction are guaranteed, and combination of hot water extraction, ultrasonic extraction and microwave extraction is efficient.
Description
Technical field
The present invention relates to the extracting method of polysaccharide, specifically a kind of method of extracting Hericium erinaceus Polysaccharides.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. has another name called Hericium erinaceus (Bull. Ex Fr.) Pers., cauliflower bacterium etc., belongs to Basidiomycetes, hedgehog fungus section, Aphyllophorales.The various nutritive ingredients such as Hericium erinaceus (Bull. Ex Fr.) Pers. rich in proteins, each seed amino acid, VITMAIN B1, vitamins C, nicotinic acid, it has multiple biological activity simultaneously, so it has raising immunizing power, improve body oxytolerant power, improve painstaking effort output and accelerate the effect such as blood circulation, also have the effects such as anticancer, anti-ageing, strengthening immunity, lowering blood glucose, blood fat.Hericium erinaceus (Bull. Ex Fr.) Pers. is the famous dual-purpose bacterium of food, medicine, and edible taste is delicious, and saying of " mountain delicacy hedgehog hydnum, seafood delights bird's nest " just arranged from ancient times.It is always run neck and neck with bear's paw, sea cucumber, shark's fin, is four world-famous large treasures, and unique pharmaceutical use is arranged again.The polysaccharide of Hericium erinaceus (Bull. Ex Fr.) Pers. is demonstrating good curative effect as the important biologically active substance of a class aspect the treatment of some human difficult and complicated illness.Showing that it has no side effect through clinical use, generally can be used for pharmacy and do the production of some health-care tonic product, is good health-nutrition food.
Summary of the invention
The invention provides a kind of method of extracting Hericium erinaceus Polysaccharides, for extraction, the Application and Development of Hericium erinaceus Polysaccharides provides condition, method is simple, and yield is high.
For achieving the above object, technical scheme of the present invention is as follows:
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross the 70-90 mesh sieve, 1:15-25 soaked 1-4 hours by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20-30 soaked 2-4 hour by weight ratio, the power of regulating microwave generator is 600-800w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 6-10min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:10-20 joins in 80-90 ℃ the hot water lixiviate 2-8 hour by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:10-20 joins in 20-40 ℃ the hot water by weight ratio, and supersound extraction 40-60 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 50-60 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt the edible ethanol of 80-90%, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 18-30 hours under 1-4 ℃ the low temperature after, with the centrifugal 20-30min of the rotating speed of 2500-4000r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 50-70 ℃, preserved according to purposes and needs.
The present invention adopts hot water lixiviate, supersound extraction and three kinds of method methods of microwave extraction that Hericium erinaceus polysaccharide is extracted, this kind method rapidly and efficiently, in conjunction with three kinds of extracting modes, the penetrable spe medium of microwave, directly act on material inside, internal temperature is risen rapidly, increase the solubleness of target substance in medium, steady quality; The microwave extracting rate of heating is fast; the heated time of material is short; also can under lower temperature condition, finish extracting operation; so can effectively protect the functional component of polysaccharide; have the superiority of joint thing, energy-saving and environmental protection, hot water lixiviate and supersound extraction are traditional extracting modes, have optimized operating parameters; guaranteed speed and the yield extracted, triplicity is more efficient.
Embodiment
Describe the present invention below by specific embodiment:
Embodiment 1
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 70 mesh sieves, 1:15 soaks 1 hour by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20 soaked 2 hours by weight ratio, the power of regulating microwave generator is 600w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 6min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:10 joined in 80 ℃ the hot water lixiviate 2 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:10 joins in 20 ℃ the hot water by weight ratio, and supersound extraction 40 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 50 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 80% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 18 hours under 1-4 ℃ the low temperature after, with the centrifugal 20min of the rotating speed of 2500r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 50 ℃, preserved according to purposes and needs.
Embodiment 2
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 90 mesh sieves, 1:25 soaks 4 hours by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:30 soaked 4 hours by weight ratio, the power of regulating microwave generator is 800w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 10min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:20 joined in 90 ℃ the hot water lixiviate 8 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:20 joins in 40 ℃ the hot water by weight ratio, and supersound extraction 60 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 60 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 90% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 30 hours under 4 ℃ the low temperature after, with the centrifugal 30min of the rotating speed of 4000r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 70 ℃, preserved according to purposes and needs.
Embodiment 3
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 80 mesh sieves, 1:25 soaks 1 hour by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:25 soaked 2 hours by weight ratio, the power of regulating microwave generator is 700w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 8min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:15 joined in 90 ℃ the hot water lixiviate 6 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:1015 joins in 30 ℃ the hot water by weight ratio, and supersound extraction 50 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 55 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 85% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 20 hours under 3 ℃ the low temperature after, with the centrifugal 25min of the rotating speed of 3000r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 50 ℃, preserved according to purposes and needs.
Embodiment 4
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 70 mesh sieves, 1:25 soaks 1 hour by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20 soaked 4 hours by weight ratio, the power of regulating microwave generator is 800w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 6min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:20 joined in 80 ℃ the hot water lixiviate 4 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:10 joins in 35 ℃ the hot water by weight ratio, and supersound extraction 55 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 50 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 90% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 18-30 hours under 1-4 ℃ the low temperature after, with the centrifugal 25min of the rotating speed of 3500r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 55 ℃, preserved according to purposes and needs.
Embodiment 5
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 70 mesh sieves, 1:15 soaks 3 hours by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20 soaked 2 hours by weight ratio, the power of regulating microwave generator is 600w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 10min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:10 joined in 80 ℃ the hot water lixiviate 2 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:15 joins in 30 ℃ the hot water by weight ratio, and supersound extraction 50 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 60 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 90% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 24 hours under 2 ℃ the low temperature after, with the centrifugal 30min of the rotating speed of 2500r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 70 ℃, preserved according to purposes and needs.
Embodiment 6
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross 90 mesh sieves, 1:25 soaks 1 hour by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20 soaked 3 hours by weight ratio, the power of regulating microwave generator is 650w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 7min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:20 joined in 90 ℃ the hot water lixiviate 6 hours by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:15 joins in 30 ℃ the hot water by weight ratio, and supersound extraction 55 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 50 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt 80% edible ethanol, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 18 hours under 4 ℃ the low temperature after, with the centrifugal 20min of the rotating speed of 4000r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 70 ℃, preserved according to purposes and needs.
Certainly, above-mentioned explanation is not to be limitation of the present invention, and the present invention also is not limited in above-mentioned giving an example, and the variation that those skilled in the art make in essential scope of the present invention, remodeling, interpolation or replacement also should belong to protection scope of the present invention.
Claims (1)
1. a method of extracting Hericium erinaceus Polysaccharides is characterized in that, is realized by the following step:
1) pretreatment: get Hericium erinaceus, be equally divided into four parts, through pulverizing, cross the 70-90 mesh sieve, 1:15-25 soaked 1-4 hours by weight ratio, and is to be extracted;
2) microwave extraction: get Hericium erinaceus a pretreated in the step 1), 1:20-30 soaked 2-4 hour by weight ratio, the power of regulating microwave generator is 600-800w, Hericium erinaceus to above-mentioned immersion carries out carrying out solid-liquid separation behind the microwave treatment 6-10min, stays mixed solution stand-by after abandoning solid substance;
3) hot water lixiviate: get two parts of pretreated Hericium erinaceus in the step 1), 1:10-20 joins in 80-90 ℃ the hot water lixiviate 2-8 hour by weight ratio, and it is stand-by to obtain mixed solution;
4) supersound extraction: get in the step 1) a pretreated Hericium erinaceus, 1:10-20 joins in 20-40 ℃ the hot water by weight ratio, and supersound extraction 40-60 minutes is regulated ph=7.5, and it is stand-by to obtain mixed solution;
5) concentrated: with above-mentioned steps 2), 3), 4) mixed solution that obtains mixes in the rearmounted Rotary Evaporators, carries out concentrating under reduced pressure at 50-60 ℃, be concentrated into 1/4 of original volume and get final product;
6) alcohol precipitation: adopt the edible ethanol of 80-90%, mix in the ratio of concentrated solution and ethanol 1:2, polysaccharide precipitation is in container base;
7) centrifugal: will add concentrated solution behind the ethanol put leave standstill 18-30 hours under 1-4 ℃ the low temperature after, with the centrifugal 20-30min of the rotating speed of 2500-4000r/min, collect Centrifuge Cup bottom polysaccharide;
8) drying: the polysaccharide of collecting is put in the electric vacunm drying case, in vacuum-drying below 50-70 ℃, preserved according to purposes and needs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210571817 CN103044563A (en) | 2012-12-26 | 2012-12-26 | Method for extracting hericium erinaceus polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210571817 CN103044563A (en) | 2012-12-26 | 2012-12-26 | Method for extracting hericium erinaceus polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103044563A true CN103044563A (en) | 2013-04-17 |
Family
ID=48057444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210571817 Pending CN103044563A (en) | 2012-12-26 | 2012-12-26 | Method for extracting hericium erinaceus polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103044563A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103819576A (en) * | 2014-03-19 | 2014-05-28 | 上海市农业科学院 | Hericium erinaceus large-molecular polysaccharide and preparation method thereof |
| CN103923221A (en) * | 2014-04-25 | 2014-07-16 | 河南中医学院 | Preparation method and application of herba ardisiae japonicae polysaccharide |
| CN104026542A (en) * | 2014-06-25 | 2014-09-10 | 江南大学 | Method for preparing lentinus edodes-flavored composite chicken powder from conventional chicken powder and lentinus edodes stems |
| CN104211827A (en) * | 2014-09-16 | 2014-12-17 | 江苏农林职业技术学院 | Method for extracting polysaccharide from hericium erinaceus mushroom dregs |
| CN104480162A (en) * | 2014-11-28 | 2015-04-01 | 哈尔滨墨医生物技术有限公司 | Method for efficiently extracting hericium erinaceus polysaccharides |
| CN106478829A (en) * | 2016-10-24 | 2017-03-08 | 上海市农业科学院 | A kind of Hericium erinaceus active polysaccharide and preparation method thereof |
-
2012
- 2012-12-26 CN CN 201210571817 patent/CN103044563A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103819576A (en) * | 2014-03-19 | 2014-05-28 | 上海市农业科学院 | Hericium erinaceus large-molecular polysaccharide and preparation method thereof |
| CN103819576B (en) * | 2014-03-19 | 2016-11-23 | 上海市农业科学院 | A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. large molecular weight polysaccharides and preparation method thereof |
| CN103923221A (en) * | 2014-04-25 | 2014-07-16 | 河南中医学院 | Preparation method and application of herba ardisiae japonicae polysaccharide |
| CN103923221B (en) * | 2014-04-25 | 2016-04-27 | 河南中医学院 | A kind of preparation method of Japanese Ardisia Herb polysaccharide and application |
| CN104026542A (en) * | 2014-06-25 | 2014-09-10 | 江南大学 | Method for preparing lentinus edodes-flavored composite chicken powder from conventional chicken powder and lentinus edodes stems |
| CN104026542B (en) * | 2014-06-25 | 2016-05-04 | 江南大学 | A kind of method of utilizing existing chicken powder and mushroom stems to prepare the compound chicken powder of mushroom taste |
| CN104211827A (en) * | 2014-09-16 | 2014-12-17 | 江苏农林职业技术学院 | Method for extracting polysaccharide from hericium erinaceus mushroom dregs |
| CN104211827B (en) * | 2014-09-16 | 2016-09-14 | 江苏农林职业技术学院 | A kind of method extracting polysaccharide from Hericium erinaceus (Bull. Ex Fr.) Pers. dreg |
| CN104480162A (en) * | 2014-11-28 | 2015-04-01 | 哈尔滨墨医生物技术有限公司 | Method for efficiently extracting hericium erinaceus polysaccharides |
| CN106478829A (en) * | 2016-10-24 | 2017-03-08 | 上海市农业科学院 | A kind of Hericium erinaceus active polysaccharide and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103044563A (en) | Method for extracting hericium erinaceus polysaccharide | |
| CN105255579B (en) | Method for extracting peony seed oil | |
| CN103131530B (en) | Preparation method for fish eye socket active grease | |
| CN105087729A (en) | Tuna bone collagen peptide preparation method | |
| CN104432126A (en) | Processing method of instant bullfrog | |
| CN105087728A (en) | Preparation method of selenium-chelated collagen peptide with tuna bone collagen serving as source | |
| CN102048020B (en) | Method for extracting yellow mealworm active proteins by using alkali-enzyme method | |
| CN105249305A (en) | Goose fat liver-containing fish flesh ball and preparation method thereof | |
| CN103315126A (en) | Preparation method for low-molecular-weight fish hydrolyzed protein powder | |
| CN105061382B (en) | Method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin | |
| CN103820517A (en) | Preparation method for yak skin micromolecular collagen capable of regulating human body energy metabolism | |
| CN105166621A (en) | Mushroom-flavored vermicelli capable of nourishing yin for moistening dryness and preparation method of mushroom-flavored vermicelli | |
| CN105294868A (en) | Extraction method for mushroom polysaccharide and preparation method for double-mushroom soup-stock essence | |
| CN104719922A (en) | Instant wood frog and production method thereof | |
| CN103494246A (en) | Salting solution and salting method of red-yolk low-salt healthcare salted duck egg | |
| CN109601956A (en) | A kind of process preparing chicken flavor composite yeast extract using Maillard reaction | |
| CN105367682A (en) | Lentinan preparation method | |
| CN104147071A (en) | Method for extracting effective components from saussurea involucrata | |
| JP5069797B2 (en) | Sea bile nutrition food and manufacturing method thereof | |
| CN103815055B (en) | Blend cooking oil of peony seed oil and tea seed oil, and preparation method thereof | |
| CN105420326A (en) | Method for extracting proteins and astaxanthin from shrimp shells through papain hydrolysis | |
| CN104522287A (en) | Preparation method and application of giant salamander albumen powder | |
| CN108094494A (en) | A kind of preparation method of flavor dried fish | |
| CN101475650A (en) | Extraction process for Hericium erinaceus polysaccharide | |
| CN106036901A (en) | Method for improving quality of aqueous extracts of shiitake mushrooms and increasing drying speed of aqueous extracts of shiitake mushrooms on basis of vacuum freezing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130417 |