CN103044149A - Nursery stock treatment medicament for preventing low-temperature stress and treatment method thereof - Google Patents
Nursery stock treatment medicament for preventing low-temperature stress and treatment method thereof Download PDFInfo
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- CN103044149A CN103044149A CN2013100171034A CN201310017103A CN103044149A CN 103044149 A CN103044149 A CN 103044149A CN 2013100171034 A CN2013100171034 A CN 2013100171034A CN 201310017103 A CN201310017103 A CN 201310017103A CN 103044149 A CN103044149 A CN 103044149A
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Abstract
The invention discloses a nursery stock treatment medicament for preventing low-temperature stress and a treatment method thereof, belonging to the technical field of garden plant protection. The nursery stock treatment medicament is characterized by being prepared by uniformly mixing the following substances: saturated lime water, gibberellin, uniconazole, potassium nitrate and riboflavin in a weight ratio of (0.21 to 0.45): (0.08 to 0.19): (0.03 to 0.09): (0.15 to 0.37): (0.13 to 0.42). According to the nursery stock treatment medicament aforementioned for preventing low-temperature stress and the treatment method thereof, the treatment medicament is low in production cost, good in surface tension and permeability, great in a protective effect on nursery stocks in case of low-temperature stress, and capable of promoting the growth of the nursery stocks, providing adequate nutrient substances, improving the own quality, the disease-resistant capacity and other capacities of the nursery stocks; and the treatment method for the nursery stocks is simple and convenient, good in effect, and without adverse effects on soil, environment and the like.
Description
Technical field
The invention belongs to ornamental plant resist technology field, be specially a kind of nursery stock treatment agent and treatment process thereof of preventing low temperature stress.
Background technology
The mechanism of the low-temperature adaptation of xylophyta is the focus of cold-resistant area research, and for xylophyta, especially the research of the domestication by low temperature of gardening plant and cold hardiness thereof has very high economic worth.Plant can show various proterties when being subject to environment stress, will cause death when serious, is embodied in the variation of some enzymes in its body.The physical signs understanding plant materialss such as cell leakage that usually can be by measuring plant materials, peroxidase (POD) activity, the free proline content degree that is afflicted by persecution, thereby learn that plant materials stands the maximum capacity of environment stress, help to inquire into it and under different growing environment conditions, be configured application.
Cytolemma is the principal passage with external environment generation exchange of substance, is that cell is experienced the most responsive position of environment-stress, also is to be subjected to freeze injury and to damage to plants caused by sudden drop in temperature and the originally position damaged.The variation of cell leakage is the key point of low temperature stress.Plasma membrane relatively thoroughly property can represent with relative conductivity, it is the endure cold important indicator of rear cellular plasm membrane permeability size of reflection plant tissue, represent that with relative conductivity it is plant identification winter resistance method commonly used that plant Permeability under injury from low temperature changes, and is widely used.Therefore, tolerance to cold and the specific conductivity of plant are inversely proportional to, and specific conductivity rises faster, and the tolerance to cold of plant is lower.Different hypothermia is processed corresponding specific conductivity carry out linear regression, the slope of the faster then straight line of conductivity variations is larger, and the cold tolerance of this kind of plant is more weak.Peroxidase (POD) is one of moiety of film securing system, can remove the active oxygen in the plant materials in adverse circumstance, reduces OH
-Formation, keep the active oxygen metabolism balance in the body, alleviate toxic substance to the murder by poisoning of viable cell, postpone and stop cyto-architectural destruction, tissue is maintained vigour, thereby makes plant restrain oneself to a certain extent the opposing environment stress.Therefore under low temperature stress, the ability that the content of POD is stood adverse circumstance to plant has certain positive correlation.Proline is important osmotic adjustment in the vegetable cell, also is considered to the product of plant under environment stress.It is a kind of water and the stronger amino acid of ability, and the increase of its content helps the stable of cell water holding and structure of biological macromolecule.Studies show that in a large number, the accumulation of proline(Pro) is relevant with the dehydration of cell, causes the accumulation of proline(Pro) owing to low temperature causes dehydration.Its accumulation be the more important thing is film fat and albumen are played a protective role except playing the osmoregulation, prevents that active oxygen is to the peroxidation of film fat and albumen.There is certain positive correlation between proline content in the Plant Under The Stress body and the plant cold resistance.Under same low temperature changed, plant leaf proline content increment was more, and then the tolerance to cold of this plant is stronger.
Summary of the invention
For problems of the prior art, the object of the invention is to design provides a kind of nursery stock treatment agent of low temperature stress and technical scheme for the treatment of process thereof of preventing, this nursery stock treatment agent production cost is low, and nursery stock is prevented and treated process effective, and little to environmental influence.
Described a kind of nursery stock treatment agent that prevents low temperature stress is characterized in that being mixed by following material and makes: saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the weight ratio of riboflavin is 0.21-0.45:0.08-0.19:0.03-0.09:0.15-0.37:0.13-0.42.
Described a kind of nursery stock treatment agent that prevents low temperature stress, it is characterized in that saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the riboflavin weight ratio is: 0.25-0.40:0.10-0.17:0.04-0.08:0.17-0.30:0.15-0.40.
Described a kind of nursery stock treatment agent that prevents low temperature stress, it is characterized in that saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the riboflavin weight ratio is: be preferably 0.28-0.32:0.12-0.16:0.05-0.07:0.20-0.28:0.17-0.35.
Described a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress is characterized in that may further comprise the steps:
1) medicament preparation: according to saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the weight ratio of riboflavin is that the ratio of 0.21-0.45:0.08-0.19:0.03-0.09:0.15-0.37:0.13-0.42 mixes it, and is for subsequent use;
2) nursery stock is processed: above-mentioned medicament is diluted to 600-1200mg/L, soak or pouring nursery stock root or to the processing of spraying of complete stool nursery stock, pouring and spray dosage are the every cube volume 80-120g of nursery stock, every re-treatment in 5-10 days once, and co-processing 2-3 time.
Described a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress is characterized in that step 2) in: medicament is diluted to 700-1100mg/L, is preferably 800-900mg/L;
Described a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress is characterized in that step 2) in: pouring and spray dosage are the every cube volume 85-110g of nursery stock, are preferably 90-100g.
Above-mentioned a kind of nursery stock treatment agent and treatment process thereof of preventing low temperature stress, contain the required nutritive element of multiple seedling growth and cold-resistant protection factor, be conducive to that nursery stock keeps out the cold, the coercing of low temperature etc., nursery stock after the cryogenic freezing erosion there are certain protection and degeneration-resistant effect, can effectively regulate growing of nursery stock, safeguard the normal operation of plant physiology and biochemistry aspect, and this treatment agent low production cost; Surface tension, good penetrability have good protective effect to nursery stock during low temperature stress, also can promote seedling growth and the sufficient nutrient material is provided, and improve quality and the ability such as disease-resistant of nursery stock self.This nursery stock treatment process is easy, effective, and soil, environment etc. is all had no adverse effects.
The percentage composition that relates in the present specification unless otherwise indicated, other the weight percentage that is pure substance.
Embodiment
Now in conjunction with embodiments of the invention and the test of pesticide effectiveness, further specify beneficial effect of the present invention.
Embodiment 1
Drop into the 28kg saturated limewater in the 100L stirring tank, slowly drop into 12kg Plant hormones regulators,gibberellins, 5kg uniconazole, 20kg saltpetre and 35kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 750mg/L, every cube volume consumption is 105g, soaks the nursery stock root, co-processing 2 times.
Embodiment 2
Drop into the 32kg saturated limewater in the 100L stirring tank, slowly drop into 16kg Plant hormones regulators,gibberellins, 7kg uniconazole, 28kg saltpetre and 17kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 800mg/L, every cube volume consumption is 100g, pouring nursery stock root, co-processing 3 times.
Embodiment 3
Drop into the 29kg saturated limewater in the 100L stirring tank, slowly drop into 13kg Plant hormones regulators,gibberellins, 5.5kg uniconazole, 22kg saltpetre and 30.5kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 850mg/L, every cube volume consumption is 95g, to the processing of spraying of complete stool nursery stock, co-processing 2 times.
Embodiment 4
Drop into the 30kg saturated limewater in the 100L stirring tank, slowly drop into 14kg Plant hormones regulators,gibberellins, 6kg uniconazole, 24kg saltpetre and 26kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 900mg/L, every cube volume consumption is 90g, soaks the nursery stock root, co-processing 3 times.
Embodiment 5
Drop into the 31kg saturated limewater in the 100L stirring tank, slowly drop into 15kg Plant hormones regulators,gibberellins, 6.5kg uniconazole, 23kg saltpetre and 24.5kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 950mg/L, every cube volume consumption is 93g, pouring nursery stock root, co-processing 3 times.
Embodiment 6
Drop into the 28.5kg saturated limewater in the 100L stirring tank, slowly drop into 15.5kg Plant hormones regulators,gibberellins, 6.2kg uniconazole, 27kg saltpetre and 22.8kg riboflavin again, stirring and dissolving mixes, the nursery stock treatment agent.Again above-mentioned medicament is diluted to 1000mg/L, every cube volume consumption is 98g, to the processing of spraying of complete stool nursery stock, co-processing 2 times.
Below further specify beneficial effect of the present invention by test
Test one:
Tested winter in 2012, in nursery, school district, Zhejiang A ﹠ F University East Lake, select respectively 10 strains growth vigorous, the 1a that growth characteristics is identical gives birth to the kadsura longepedunculata seedling, then use embodiment gained medicament, process according to concrete processing requirements, after whole processing finish, put into respectively growth case and temperature-controlled box and carry out artificial freezing treatment, and temperature is adjusted into 0 ℃ respectively,-10 ℃ and-20 ℃, random alignment is to the plant of different treatment, choose and grow the normal functional leaf in branch middle part, regularly mix sampling, get 10 blades at every turn, measure respectively relative physiologic index.Repeat 3 times.Observe simultaneously the morphological specificity of plant during sampling.Cell leakage adopts conductivity method; The POD enzymic activity adopts the methyl catechol determination of color; Free proline content adopts improved ninhydrin colorimetry to measure.Testing data all represents with mean+SD, carries out the data drawing list analysis with Excel.Test-results sees Table 1.
Table 1 shows that a series of physical signs such as peroxidase activity, specific conductivity and free proline content of kadsura longepedunculata seedling under frozen stress after embodiment 1-6 processes are all normally stable, plant is for saving self growth needs from damage under the low temperature stress, produce a certain amount of peroxidase to adapt to the variation of external environment, proline content also slightly increases synchronously, and specific conductivity rises slowly, show that all cold tolerance is good, embodiment 1-6 all shows the effect of protecting preferably and resisting low temperature stress.
Claims (6)
1. nursery stock treatment agent that prevents low temperature stress is characterized in that being mixed by following material and makes: saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the weight ratio of riboflavin is 0.21-0.45:0.08-0.19:0.03-0.09:0.15-0.37:0.13-0.42.
2. a kind of nursery stock treatment agent that prevents low temperature stress as claimed in claim 1, it is characterized in that saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the riboflavin weight ratio is: 0.25-0.40:0.10-0.17:0.04-0.08:0.17-0.30:0.15-0.40.
3. a kind of nursery stock treatment agent that prevents low temperature stress as claimed in claim 1, it is characterized in that saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the riboflavin weight ratio is: be preferably 0.28-0.32:0.12-0.16:0.05-0.07:0.20-0.28:0.17-0.35.
4. adopt a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress claimed in claim 1, it is characterized in that may further comprise the steps:
1) medicament preparation: according to saturated limewater: Plant hormones regulators,gibberellins: uniconazole: saltpetre: the weight ratio of riboflavin is that the ratio of 0.21-0.45:0.08-0.19:0.03-0.09:0.15-0.37:0.13-0.42 mixes it, and is for subsequent use;
2) nursery stock is processed: above-mentioned medicament is diluted to 600-1200mg/L, soak or pouring nursery stock root or to the processing of spraying of complete stool nursery stock, pouring and spray dosage are the every cube volume 80-120g of nursery stock, every re-treatment in 5-10 days once, and co-processing 2-3 time.
5. a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress as claimed in claim 4 is characterized in that step 2) in: medicament is diluted to 700-1100mg/L, is preferably 800-900mg/L.
6. a kind for the treatment of process of preventing the nursery stock treatment agent of low temperature stress as claimed in claim 4 is characterized in that step 2) in: pouring and spray dosage are the every cube volume 85-110g of nursery stock, are preferably 90-100g.
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| CN201310017103.4A CN103044149B (en) | 2013-01-17 | 2013-01-17 | The seedling treatment medicament and its processing method of a kind of prevention low temperature stress |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686178A (en) * | 2015-03-25 | 2015-06-10 | 大连金三元生态园林工程股份有限公司 | Construction method of drought resistant and cold resistant nursery stock planting |
| CN110278798A (en) * | 2019-07-22 | 2019-09-27 | 青岛农业大学 | A kind of medicament method of production reducing corn low temperature stress |
| CN114431240A (en) * | 2022-01-24 | 2022-05-06 | 上海市农业科学院 | Pharmaceutical composition for rapidly protecting cold resistance of tomatoes, preparation method and application |
Citations (2)
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| CN102715058A (en) * | 2012-06-29 | 2012-10-10 | 浙江农林大学 | Method for improving garden plant transplanting survival rate |
| CN102715202A (en) * | 2012-06-13 | 2012-10-10 | 浙江农林大学 | Modified drug for improving cold resistance of horsetail beefwood |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715202A (en) * | 2012-06-13 | 2012-10-10 | 浙江农林大学 | Modified drug for improving cold resistance of horsetail beefwood |
| CN102715058A (en) * | 2012-06-29 | 2012-10-10 | 浙江农林大学 | Method for improving garden plant transplanting survival rate |
Non-Patent Citations (2)
| Title |
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| 杨飞飞: "伸展素与核黄素对植物生长与抗病防卫的调控作用", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686178A (en) * | 2015-03-25 | 2015-06-10 | 大连金三元生态园林工程股份有限公司 | Construction method of drought resistant and cold resistant nursery stock planting |
| CN110278798A (en) * | 2019-07-22 | 2019-09-27 | 青岛农业大学 | A kind of medicament method of production reducing corn low temperature stress |
| CN114431240A (en) * | 2022-01-24 | 2022-05-06 | 上海市农业科学院 | Pharmaceutical composition for rapidly protecting cold resistance of tomatoes, preparation method and application |
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| CN103044149B (en) | 2017-09-19 |
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