CN103033642A - Measuring method of crawling speed of sea urchin - Google Patents
Measuring method of crawling speed of sea urchin Download PDFInfo
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- CN103033642A CN103033642A CN201210576862XA CN201210576862A CN103033642A CN 103033642 A CN103033642 A CN 103033642A CN 201210576862X A CN201210576862X A CN 201210576862XA CN 201210576862 A CN201210576862 A CN 201210576862A CN 103033642 A CN103033642 A CN 103033642A
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- 241000257465 Echinoidea Species 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000009193 crawling Effects 0.000 title abstract 7
- 239000011521 glass Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000013535 sea water Substances 0.000 claims abstract description 5
- 239000004744 fabric Substances 0.000 claims description 4
- 238000000691 measurement method Methods 0.000 abstract 1
- 230000035790 physiological processes and functions Effects 0.000 abstract 1
- 238000005259 measurement Methods 0.000 description 4
- 241000258955 Echinodermata Species 0.000 description 1
- 241001298668 Heliocidaris crassispina Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000013456 study Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
海胆爬行速度测量方法，其特征在于：将待测海胆放入盛有海水的玻璃水缸中，玻璃水缸的底部设置有边长为5cm正方形的网格，在玻璃水缸的正上方设置有摄像头，对海胆的运动过程进行拍摄，在拍摄的视频中选取海胆沿直线爬行的一段视频，对这段视频的第一帧和最后一帧画面进行截图，并将两幅截图叠加在一起，利用图像测量软件以网格作为参照计算出海胆的运动距离L，通过截取的第一帧画面与最后一帧画面的时间间隔计算海胆的运动时间T，计算得出海胆的爬行速度V=L/T，至少选取三段海胆沿直线爬行的视频，并利用上述方法计算每次的爬行速度，取平均值。这是一种简单易行，便于操作，既不会影响海胆正常生长和生理状态，又能准确测量海胆爬行速度的方法。
The sea urchin crawling speed measurement method is characterized in that: the sea urchin to be measured is put into a glass water tank filled with sea water, the bottom of the glass water tank is provided with a grid with a side length of 5 cm square, and a grid is arranged directly above the glass water tank. The camera shoots the movement process of sea urchins, selects a video of sea urchins crawling along a straight line in the captured video, takes screenshots of the first frame and the last frame of this video, and superimposes the two screenshots together, using The image measurement software uses the grid as a reference to calculate the movement distance L of the sea urchin, and calculates the movement time T of the sea urchin through the time interval between the first and last frame of the intercepted picture, and calculates the crawling speed of the sea urchin V=L/T , select at least three videos of sea urchins crawling along a straight line, and use the above method to calculate the crawling speed each time, and take the average value. This is a method that is simple, easy to operate, does not affect the normal growth and physiological state of the sea urchin, and can accurately measure the crawling speed of the sea urchin.
Description
Technical field
The present invention relates to a kind of biological parameter measuring method of sea urchin, particularly a kind of sea urchin creep speed measuring method.
Background technology
Sea urchin is the general designation of Echinodermata echinoid, and existing sea urchin has kind more than 850 approximately in the world, and China coast has kind more than 150 approximately.Common are horsedung sea urchin, Dalian Anthocidaris crassispina and heart urchin etc.Sea urchin has higher edible, medicinal and scientific research and is worth, and the gonad delicious flavour of Partial Species is nutritious.Along with the raising of China's living standards of the people and the rising of international market demand, sea urchin has become the important object of China coast aquaculture and enhancement releasing in recent years.For cultured output and the enhancement effect that improves sea urchin, Chinese scholars has been carried out a large amount of relevant rudimentary Journal of Sex Research about aspects such as sea urchin biology, ecology and behaviouristics.
In the correlative studys such as the ecology of sea urchin and behaviouristics, creep speed is a very important behavioral ecology index.Sea urchin is creeped by the motion of sucker and quil, and creep speed is slow.But the creep speed of sea urchin and its existence are closely related, and what directly affect sea urchin ingests, hides harmful animal and uncomfortable environment and distribution etc.Yet, also do not have up to now a kind of method of Measurement accuracy sea urchin creep speed.
Summary of the invention
The present invention is in order to solve the existing above-mentioned deficiency of prior art, proposes a kind of simplely, and convenient operation neither can affect sea urchin normal growth and physiological status, method that again can Measurement accuracy sea urchin creep speed.
Technical solution of the present invention is: a kind of sea urchin creep speed measuring method, it is characterized in that: sea urchin to be measured is put into the glass water vat that fills seawater, it is the foursquare grid of 5cm that the bottom of glass water vat is provided with the length of side, directly over the glass water vat, be provided with camera, motion process to sea urchin is taken, in the video of taking, choose sea urchin along one section video of rectilinear creeping, the first frame and last frame picture to this section video carry out sectional drawing, and two width of cloth sectional drawings are superimposed, utilize image measurement software to calculate the move distance L of sea urchin as reference with grid, the run duration T of the time interval calculating sea urchin of the first frame picture and last frame picture by intercepting, calculate the creep speed V=L/T of sea urchin, at least choose three sections sea urchins along the video of rectilinear creeping, and utilize said method to calculate each creep speed, average.
The present invention compares with prior art, has following advantage:
Sea urchin creep speed measuring method disclosed in this invention, easy to operate, simple, this method is by means of apparatus for making a video recording and image measurement software, can measure very accurately creep distance and the crawl time of sea urchin, and calculate the creep speed of sea urchin according to speed formula.In whole measuring process, sea urchin can not be subject to any chemical affect or physical injury fully in the raw, and the sea urchin after measuring is put in the nature seawater, can recover at once to move and ingest, and does not affect its self-sow.Therefore this method has possessed safely, measures accurately, and the advantages such as simple to operate, convenient and good reproducibility are for halobiontic creep speed measurement provides brand-new thinking.
Description of drawings
Fig. 1 is the picture sectional drawing of sea urchin when beginning rectilinear motion.
Picture sectional drawing when Fig. 2 is sea urchin end rectilinear motion.
Fig. 3 is the spliced map that sea urchin begins and finish straight-line two width of cloth sectional drawings.
Embodiment
Below in conjunction with description of drawings the specific embodiment of the present invention.
The sea urchin of at first will living is put into the glass water vat that seawater is housed, be provided with the square net that the length of side is 5cm in the bottom of glass water vat, directly over the glass water vat, be provided with camera, utilize camera that the motion process of sea urchin is taken, and utilize video jukebox software to play this section video, choose sea urchin along one section video of rectilinear creeping, the first frame (beginning rectilinear creeping to this section video, as shown in Figure 1) and last frame (finish rectilinear creeping, picture as shown in Figure 2) carries out sectional drawing, then utilize image processing software to be superimposed (as shown in Figure 3) in this two width of cloth picture, in same figure, show simultaneously sea urchin and begin two images of creeping and finishing to creep, then utilize image measurement software with the grid in the picture as object of reference, calculate the move distance L of sea urchin; Then calculate the first frame picture intercept and the time interval between the last frame picture, run duration T for sea urchin, the calculating of the run duration T here is for more accurate, here adopt the interval frame number between the first frame and the last frame divided by the mode of video camera per second record frame number time T to be calculated, then utilize speed formula V=L/T to calculate the straight-line speed of this sea urchin;
Repeat according to the method described above at least three operations, calculate respectively the straight-line velocity amplitude of sea urchin each time, then average.
Concrete measurement result is as shown in table 1.
Table 1
。
Claims (1)
1. sea urchin creep speed measuring method, it is characterized in that: sea urchin to be measured is put into the glass water vat that fills seawater, it is the foursquare grid of 5cm that the bottom of glass water vat is provided with the length of side, directly over the glass water vat, be provided with camera, motion process to sea urchin is taken, in the video of taking, choose sea urchin along one section video of rectilinear creeping, the first frame and last frame picture to this section video carry out sectional drawing, and two width of cloth sectional drawings are superimposed, utilize image measurement software to calculate the move distance L of sea urchin as reference with grid, the run duration T of the time interval calculating sea urchin of the first frame picture and last frame picture by intercepting, calculate the creep speed V=L/T of sea urchin, at least choose three sections sea urchins along the video of rectilinear creeping, and utilize said method to calculate each creep speed, average.
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| CN201210576862XA CN103033642A (en) | 2012-12-27 | 2012-12-27 | Measuring method of crawling speed of sea urchin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
| CN114018589A (en) * | 2021-10-25 | 2022-02-08 | 中汽研汽车检验中心（天津）有限公司 | Method and device for determining air bag ejection speed, electronic equipment and medium |
| CN114500882A (en) * | 2022-01-24 | 2022-05-13 | 中国科学院烟台海岸带研究所 | A method for measuring behavioral parameters of sports animals |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4290693A (en) * | 1978-03-31 | 1981-09-22 | Siemens Aktiengesellschaft | Arrangement for measuring the range or speed of an object |
| CN102297865A (en) * | 2011-05-27 | 2011-12-28 | 宁波大学 | Biological water quality monitoring system by fish behaviors and monitoring method thereof |
-
2012
- 2012-12-27 CN CN201210576862XA patent/CN103033642A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4290693A (en) * | 1978-03-31 | 1981-09-22 | Siemens Aktiengesellschaft | Arrangement for measuring the range or speed of an object |
| CN102297865A (en) * | 2011-05-27 | 2011-12-28 | 宁波大学 | Biological water quality monitoring system by fish behaviors and monitoring method thereof |
Non-Patent Citations (3)
| Title |
|---|
| KAZUTAKA YANASE ET AL.: "nfluence of water temperature and fish length on the maximum swimming speed of sand flathead, Platycephalus bassensis: Implications for trawl selectivity", 《FISHERIES RESEARCH》, no. 84, 31 December 2007 (2007-12-31), pages 180 - 188 * |
| 于晓明等: "鱼类游泳能力测定方法的研究进展", 《南方水产科学》, vol. 7, no. 4, 31 August 2011 (2011-08-31), pages 76 - 84 * |
| 无: "《百度文库》", 31 December 2011, article "实验方法" * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
| CN114018589A (en) * | 2021-10-25 | 2022-02-08 | 中汽研汽车检验中心（天津）有限公司 | Method and device for determining air bag ejection speed, electronic equipment and medium |
| CN114018589B (en) * | 2021-10-25 | 2024-03-15 | 中汽研汽车检验中心（天津）有限公司 | Method and device for determining airbag ejection speed, electronic equipment and medium |
| CN114500882A (en) * | 2022-01-24 | 2022-05-13 | 中国科学院烟台海岸带研究所 | A method for measuring behavioral parameters of sports animals |
| CN114500882B (en) * | 2022-01-24 | 2023-12-15 | 中国科学院烟台海岸带研究所 | A method for measuring behavioral parameters of moving animals |
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Application publication date: 20130410 |